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    • 1. 发明申请
    • METHODS AND COMPOSITIONS FOR THE CRYOPRESERVATION OF DUCKWEED
    • DUCKWEED的CRYOPRESERVATION的方法和组合物
    • US20120190004A1
    • 2012-07-26
    • US13379959
    • 2010-06-22
    • John L. ParsonsVincent Wingate
    • John L. ParsonsVincent Wingate
    • A01N1/02
    • A01N3/00
    • The present invention describes methods for the cryopreservation of duckweed plants and duckweed plant tissues. The methods comprise freezing a dehydrated duckweed frond colony to a cryopreservative temperature to obtain a frozen frond colony comprising at least one cryopreserved duckweed plant or a cryopreserved duckweed plant tissue. The method can comprise a dehydration step whereby a duckweed frond colony is dehydrated, and in some embodiments, can further comprise a dormancy-induction step prior to or during the dehydration step. The method further can further comprise a recovery step, wherein the frozen frond colony is thawed and a viable duckweed plant or duckweed plant tissue is recovered. Cryopreserved duckweed plants and duckweed plant tissues, and viable duckweed plants and duckweed tissues recovered therefrom are also provided. In some embodiments, the duckweed frond colony, duckweed plant, and duckweed tissue comprise a heterologous polynucleotide of interest, which can encode a heterologous polypeptide of interest.
    • 本发明描述了用于冷冻保存浮萍植物和浮萍植物组织的方法。 所述方法包括将脱水的浮萍绒毛菌落冻结至冷冻保存温度,以获得包含至少一个冷冻保存的浮萍植物或冷冻保存的浮萍植物组织的冷冻的叶状集落。 该方法可以包括脱水步骤,其中浮萍季殖群被脱水,并且在一些实施方案中,可以在脱水步骤之前或期间进一步包括休眠诱导步骤。 该方法还可以包括回收步骤,其中冷冻的frond菌落被解冻,并且回收可行的浮萍植物或浮萍植物组织。 还提供冷冻保鲜的浮萍植物和浮萍植物组织,以及从其中回收的活的浮萍植物和浮萍组织。 在一些实施方案中,浮萍属植物,浮萍植物和浮萍组织包含可以编码感兴趣的异源多肽的异源多核苷酸。