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1. 发明授权

US08383385B2 Variants of glycoside hydrolases 有权
标题翻译：糖苷水解酶的变体
公开(公告)号：US08383385B2
公开(公告)日：2013-02-26
申请号：US13009524
申请日：2011-01-19
申请人： Sarah Teter , Connie Ward , Joel Cherry , Aubrey Jones , Paul Harris , Jung Yi
发明人： Sarah Teter , Connie Ward , Joel Cherry , Aubrey Jones , Paul Harris , Jung Yi
IPC分类号： C12N9/42 , C12S3/00 , C12S3/02 , C12P19/14 , C11D3/386
CPC分类号： C12N9/2437 , C11D3/38645 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y302/01091 , C12Y302/01176
摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译：本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代，和 486，SEQ ID NO：2的氨基酸1至513，并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

2. 发明申请

US20110159570A1 Method for preparing maltogenic alpha-amylase variants 有权
标题翻译：麦芽糖α-淀粉酶变体的制备方法
公开(公告)号：US20110159570A1
公开(公告)日：2011-06-30
申请号：US13010219
申请日：2011-01-20
申请人： Joel Cherry , Allan Svendsen , Carsten Andersen , Lars Beier , Torben Peter Frandsen
发明人： Joel Cherry , Allan Svendsen , Carsten Andersen , Lars Beier , Torben Peter Frandsen
IPC分类号： C12N9/28
CPC分类号： C12N9/2417 , A21D2/26 , A21D8/042
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.
摘要翻译：基于麦芽糖α-淀粉酶Novamyl的三维结构，本发明人已经修饰了产麦芽糖α-淀粉酶的氨基酸序列以获得具有改进性质的变体。这些变体具有改变的物理化学性质，例如改变的pH最佳，改善的热稳定性，增加的比活性，改变的切割模式或增加降低淀粉退化或面包陈化的能力。

3. 发明申请

US20070015677A1 Variant subtilising enzymes (subtilases) 失效
标题翻译：变异的枯草芽孢杆菌（subtilases）
公开(公告)号：US20070015677A1
公开(公告)日：2007-01-18
申请号：US10555484
申请日：2004-05-06
申请人： Stefan Minning , Jurgen Carsten Franz Knotzel , Niels Sorensen , Jon Ness , Mark Welch , Lorraine Giver , Joel Cherry , Torben Borchert , Jeremy Minshull
发明人： Stefan Minning , Jurgen Carsten Franz Knotzel , Niels Sorensen , Jon Ness , Mark Welch , Lorraine Giver , Joel Cherry , Torben Borchert , Jeremy Minshull
IPC分类号： C11D3/386 , C07H21/04 , C12P21/06 , C12N9/54 , C12N15/74
CPC分类号： C11D3/38636 , C11D3/38681 , C12N9/52 , Y02P20/582
摘要： Novel subtilases having an improved wash performance on egg stains. These subtilases are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.
摘要翻译：具有改进的蛋渍洗涤性能的新型枯草芽孢杆菌。当用于例如蛋白质时，这些脱水酶可用于在蛋渍上表现出优异或改善的洗涤性能。清洁剂或洗涤剂组合物，例如衣物洗涤剂组合物和洗碟组合物，包括自动洗碗组合物。

4. 发明授权

US5965418A Haloperoxidases from Curvularia verruculosa and nucleic acids encoding same 失效
标题翻译：来自曲霉疣孢菌的卤素过氧化物酶和编码相同的核酸
公开(公告)号：US5965418A
公开(公告)日：1999-10-12
申请号：US842799
申请日：1997-04-16
申请人： Claus Fuglsang , Karen Oxenb.o slashed.ll , Torben Halkier , Randy M. Berka , Joel Cherry
发明人： Claus Fuglsang , Karen Oxenb.o slashed.ll , Torben Halkier , Randy M. Berka , Joel Cherry
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N9/08 , C12N15/52 , C12N15/53 , C12N15/80 , C12P21/02 , C12R1/645 , C12N15/63
CPC分类号： C12N9/0065 , Y10S435/911
摘要： The present invention relates to Curvularia verruculosa haloperoxidases and isolated nucleic acid fragments comprising nucleic acid sequences encoding the haloperoxidases as well as nucleic acid constructs, vectors, and recombinant host cells comprising the nucleic acid sequences. The invention also relates to methods for recombinant production of the haloperoxidase. The invention further relates to compositions comprising the haloperoxidases and methods of using the compositions for killing microbial cells or inhibiting growth of microbial cells.
摘要翻译：本发明涉及疣状弧菌弧菌过氧化物酶和包含编码卤代过氧化物酶的核酸序列以及包含核酸序列的核酸构建体，载体和重组宿主细胞的分离的核酸片段。本发明还涉及重组生产卤代过氧化物酶的方法。本发明还涉及包含卤代过氧化物酶的组合物和使用该组合物杀死微生物细胞或抑制微生物细胞生长的方法。

5. 发明授权

US08546099B2 Processes for preparing a dough or a baked product using maltogenic alpha-amylase variants 有权
标题翻译：使用产麦芽糖α-淀粉酶变体制备面团或烘焙产品的方法
公开(公告)号：US08546099B2
公开(公告)日：2013-10-01
申请号：US13533055
申请日：2012-06-26
申请人： Joel Cherry , Allan Svendsen , Carsten Andersen , Lars Beier , Torben Peter Frandsen
发明人： Joel Cherry , Allan Svendsen , Carsten Andersen , Lars Beier , Torben Peter Frandsen
IPC分类号： C12Q1/40 , C12N9/00 , C12N9/28 , C07H21/04
CPC分类号： C12N9/2417 , A21D2/26 , A21D8/042
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.
摘要翻译：基于麦芽糖α-淀粉酶Novamyl的三维结构，本发明人已经修饰了产麦芽糖α-淀粉酶的氨基酸序列以获得具有改进性质的变体。这些变体具有改变的物理化学性质，例如改变的pH最佳，改善的热稳定性，增加的比活性，改变的切割模式或增加降低淀粉退化或面包陈化的能力。

6. 发明授权

US07943350B2 Methods for degrading lignocellulosic materials 有权
标题翻译：降解木质纤维素材料的方法
公开(公告)号：US07943350B2
公开(公告)日：2011-05-17
申请号：US11821438
申请日：2007-06-22
申请人： Elena Vlasenko , Joel Cherry , Feng Xu
发明人： Elena Vlasenko , Joel Cherry , Feng Xu
IPC分类号： C12P19/04
CPC分类号： C12P19/14 , C12P7/06 , C12P13/04 , Y02E50/17 , Y02E50/343
摘要： The present invention relates to methods for degrading a lignocellulosic material, comprising: treating the lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying a lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant; (b) fermenting the saccharified lignocellulosic material of step (a) with one or more fermenting microorganisms; and (c) recovering the organic substance from the fermentation.
摘要翻译：本发明涉及降解木质纤维素材料的方法，包括：在至少一种表面活性剂存在下，用有效量的一种或多种纤维素分解酶处理木质纤维素材料，所述表面活性剂选自仲醇乙氧基化物，脂肪醇乙氧基化物，壬基酚乙氧基化物，十三烷基乙氧基化物和聚氧乙烯醚，其中与不存在表面活性剂相比，表面活性剂的存在增加了木质纤维素材料的降解。本发明还涉及生产有机物质的方法，其包括：（a）在至少一种表面活性剂存在下，用有效量的一种或多种纤维素分解酶对木质纤维素材料进行糖化，所述表面活性剂选自仲醇乙氧基化物，脂肪醇乙氧基化物，壬基酚乙氧基化物，十三烷基乙氧基化物和聚氧乙烯醚，其中与不存在表面活性剂相比，表面活性剂的存在增加了木质纤维素材料的降解; （b）用一种或多种发酵微生物发酵步骤（a）的糖化木质纤维素材料; 和（c）从发酵中回收有机物质。

7. 发明授权

US07908094B2 Method for preparing maltogenic alpha-amylase variants 有权
公开(公告)号：US07908094B2
公开(公告)日：2011-03-15
申请号：US12413758
申请日：2009-03-30
申请人： Joel Cherry , Allan Svendsen , Carsten Andersen , Lars Beier , Torben Peter Frandsen
发明人： Joel Cherry , Allan Svendsen , Carsten Andersen , Lars Beier , Torben Peter Frandsen
IPC分类号： G01N33/48 , G01N31/00 , C12P21/06 , C12N9/00 , C12N9/28 , C07K14/00 , C07H21/04
CPC分类号： C12N9/2417 , A21D2/26 , A21D8/042
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.

8. 发明申请

US20050164355A1 Methods for degrading lignocellulosic materials 有权
标题翻译：降解木质纤维素材料的方法
公开(公告)号：US20050164355A1
公开(公告)日：2005-07-28
申请号：US11036871
申请日：2005-01-14
申请人： Elena Vlasenko , Joel Cherry , Feng Xu
发明人： Elena Vlasenko , Joel Cherry , Feng Xu
IPC分类号： C12P7/06 , C12P7/18 , C12P7/36 , C12P7/40 , C12P13/04 , C12P19/04 , C12P19/14
CPC分类号： C12P19/14 , C12P7/06 , C12P13/04 , Y02E50/17 , Y02E50/343
摘要： The present invention relates to methods for degrading a lignocellulosic material, comprising: treating the lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying a lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant; (b) fermenting the saccharified lignocellulosic material of step (a) with one or more fermentating microoganisms; and (c) recovering the organic substance from the fermentation.
摘要翻译：本发明涉及降解木质纤维素材料的方法，包括：在至少一种表面活性剂存在下，用有效量的一种或多种纤维素分解酶处理木质纤维素材料，所述表面活性剂选自仲醇乙氧基化物，脂肪醇乙氧基化物，壬基酚乙氧基化物，十三烷基乙氧基化物和聚氧乙烯醚，其中与不存在表面活性剂相比，表面活性剂的存在增加了木质纤维素材料的降解。本发明还涉及生产有机物质的方法，其包括：（a）在至少一种表面活性剂存在下，用有效量的一种或多种纤维素分解酶对木质纤维素材料进行糖化，所述表面活性剂选自仲醇乙氧基化物，脂肪醇乙氧基化物，壬基酚乙氧基化物，十三烷基乙氧基化物和聚氧乙烯醚，其中与不存在表面活性剂相比，表面活性剂的存在增加了木质纤维素材料的降解; （b）用一种或多种发酵微生物发酵步骤（a）的糖化木质纤维素材料; 和（c）从发酵中回收有机物质。

9. 发明授权

US6162628A Maltogenic alpha-amylase variants 有权
标题翻译：产麦芽糖α-淀粉酶变体
公开(公告)号：US6162628A
公开(公告)日：2000-12-19
申请号：US386607
申请日：1999-08-31
申请人： Joel Cherry , Allan Svendsen , Carsten Andersen , Lars Beier , Torben Peter Frandsen
发明人： Joel Cherry , Allan Svendsen , Carsten Andersen , Lars Beier , Torben Peter Frandsen
IPC分类号： C12N9/28 , A21D8/04 , C12N1/15 , C12N1/21 , C12N15/09 , C12N15/56 , C12R1/07
CPC分类号： C12N9/2417 , A21D8/042 , C07K2299/00
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.
摘要翻译：基于麦芽糖α-淀粉酶Novamyl的三维结构，本发明人已经修饰了产麦芽糖α-淀粉酶的氨基酸序列以获得具有改进性质的变体。这些变体具有改变的物理化学性质，例如改变的pH最佳，改善的热稳定性，增加的比活性，改变的切割模式或增加降低淀粉退化或面包陈化的能力。

10. 发明授权

US07932073B2 Variants of glycoside hydrolases 有权
标题翻译：糖苷水解酶的变体
公开(公告)号：US07932073B2
公开(公告)日：2011-04-26
申请号：US11891249
申请日：2007-08-08
申请人： Sarah Teter , Connie Ward , Joel Cherry , Aubrey Jones , Paul Harris , Jung Yi
发明人： Sarah Teter , Connie Ward , Joel Cherry , Aubrey Jones , Paul Harris , Jung Yi
IPC分类号： C12N9/42 , C12S3/00 , C11D3/00
CPC分类号： C12N9/2437 , C11D3/38645 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y302/01091 , C12Y302/01176
摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译：本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代，和 486，SEQ ID NO：2的氨基酸1至513，并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

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