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1. 发明申请

US20120325669A1 NANOHAIR STRUCTURE AND AN APPLICATION THEREFOR 审中-公开
标题翻译： NANOHAIR结构及其应用
公开(公告)号：US20120325669A1
公开(公告)日：2012-12-27
申请号：US13225167
申请日：2011-09-02
申请人： Young Keun Kim , Jee Won Lee , Jin Seung Park , Moon Kyu Cho , Eun Jung Lee
发明人： Young Keun Kim , Jee Won Lee , Jin Seung Park , Moon Kyu Cho , Eun Jung Lee
IPC分类号： C25B9/00 , C25D5/48 , G01N27/30 , C25D5/02 , C25B11/02 , B82Y5/00 , B82Y99/00 , B82Y40/00 , B82Y30/00
CPC分类号： G01N27/327 , B82Y30/00 , C07K14/005 , C07K14/31 , C12N2730/10122 , C12N2740/16122 , C25D5/022
摘要： There is provided a nanohair structure with the nanowires exposed on a nanotemplate; the method thereof; and a three-dimensional nanostructure-based sensor with ultra-sensitivity and greatly increased three-dimensional surface-to-volume ratio which immobilizes bio-nanoparticles to the nanohair structure and arranges antibodies to the nano surface with the controlled orientation by physical interaction.
摘要翻译：提供纳米线结构，纳米线暴露在纳米模板上; 其方法; 以及具有超灵敏度和大大提高的三维表面体积比的三维纳米结构传感器，其将生物纳米颗粒固定到纳米结构结构，并通过物理相互作用以受控的取向将抗体排列到纳米表面。

2. 发明申请

US20100210827A1 Preparation Method Of Recombinant Protein By Use Of A Fusion Expression Partner 审中-公开
标题翻译：利用融合表达合成子制备重组蛋白的方法
公开(公告)号：US20100210827A1
公开(公告)日：2010-08-19
申请号：US12676982
申请日：2008-09-05
申请人： Jeewon Lee , Kyung-Yeon Han , Jong-Am Song , Jin-Seung Park , Hyuk-Seong Seo , Keum-Young Ahn , Eun-Jjung Lee , Jong-Hwan Lee , Soo-Jung Kwon
发明人： Jeewon Lee , Kyung-Yeon Han , Jong-Am Song , Jin-Seung Park , Hyuk-Seong Seo , Keum-Young Ahn , Eun-Jjung Lee , Jong-Hwan Lee , Soo-Jung Kwon
IPC分类号： C07K14/00 , C12P21/00 , C12N9/00 , C12N1/00 , C12N15/63
CPC分类号： C12N15/67 , C12N15/64
摘要： The present invention relates to a preparation method using a fusion expression partner. The method includes preparing a polynucleotide encoding a fusion expression partner selected from the group consisting of SlyD (FKBR type peptidyl prolyl cis-trans isomerase). Crr (glucose-specific phosphotransferase (PTS) enzyme IIA component). RpoS (RNA polymerase sigma factor) PotD (Spermidine/putrescine-binding periplasmic protein), and RpoA (RNA polymerase alpha subunit), and an expression vector linking a polyDNA fragment of a heterologous protein, preparing a transformant by introducing the expression vector into a host cell, inducing the expression of a recombinant protein by culturing a transformant, and obtaining the expression. In the preparation method of the recombinant protein, the heterologous protein may enhance the water-solubility and folding of the recombinant protein, overcome the limitations about the water-solubility and folding which the conventional fusion expression partners have, and be used widely in the production of pharmaceutical and industrial proteins.
摘要翻译：本发明涉及使用融合表达配偶体的制备方法。该方法包括制备编码选自SlyD（FKBR型肽基脯氨酰顺反异构酶）的融合表达伴侣的多核苷酸。 Crr（葡萄糖特异性磷酸转移酶（PTS）酶IIA成分）。 RpoS（RNA聚合酶sigma因子）PotD（亚精胺/腐胺结合周质蛋白）和RpoA（RNA聚合酶α亚基）和连接异源蛋白质的多聚DNA片段的表达载体，通过将表达载体引入到宿主细胞，通过培养转化体诱导重组蛋白的表达，获得表达。在重组蛋白的制备方法中，异源蛋白可增强重组蛋白的水溶性和折叠，克服了常规融合表达伴侣具有的水溶性和折叠的局限性，并广泛用于生产的药物和工业蛋白质。

3. 发明授权

US08586370B2 Chimeric protein, method for manufacturing the same, nano-sensor in which the chimeric protein is fixed, and application thereof 有权
标题翻译：嵌合蛋白，其制造方法，嵌合蛋白质固定的纳米传感器及其应用
公开(公告)号：US08586370B2
公开(公告)日：2013-11-19
申请号：US13225088
申请日：2011-09-02
申请人： Jee Won Lee , Young Keun Kim , Moon Kyu Cho , Jin Seung Park , Eun Jung Lee
发明人： Jee Won Lee , Young Keun Kim , Moon Kyu Cho , Jin Seung Park , Eun Jung Lee
IPC分类号： G01N21/64 , B82Y15/00
CPC分类号： G01N27/327 , B82Y30/00 , C07K14/005 , C07K14/31 , C12N2730/10122 , C12N2740/16122 , C25D5/022
摘要： There is provided a chimeric protein of capsid protein of Hepatitis B virus (HBV) and B domain of Staphylococcal protein A (SPAB); a method for fabricating the same; the substrate and nanosensor immobilized with HBV-derived chimeric protein; and the use of the above nanosensor.
摘要翻译：提供乙型肝炎病毒（HBV）衣壳蛋白和葡萄球菌蛋白A（SPAB）B结构域的嵌合蛋白; 其制造方法; 固定有HBV衍生嵌合蛋白的底物和纳米传感器; 并使用上述纳米传感器。

4. 发明申请

US20120149120A1 CHIMERIC PROTEIN, METHOD FOR MANUFACTURING THE SAME, NANO-SENSOR IN WHICH THE CHIMERIC PROTEIN IS FIXED, AND APPLICATION THEREOF 有权
标题翻译：重要蛋白质，其制备方法，固定蛋白质的纳米传感器及其应用
公开(公告)号：US20120149120A1
公开(公告)日：2012-06-14
申请号：US13225088
申请日：2011-09-02
申请人： Jee Won Lee , Young Keun Kim , Moon Kyu Cho , Jin Seung Park , Eun Jung Lee
发明人： Jee Won Lee , Young Keun Kim , Moon Kyu Cho , Jin Seung Park , Eun Jung Lee
IPC分类号： G01N21/64 , C12P21/00 , C07K2/00 , C07K17/08 , B82Y15/00
CPC分类号： G01N27/327 , B82Y30/00 , C07K14/005 , C07K14/31 , C12N2730/10122 , C12N2740/16122 , C25D5/022
摘要： There is provided a chimeric protein of capsid protein of Hepatitis B virus (HBV) and B domain of Staphylococcal protein A (SPAB); a method for fabricating the same; the substrate and nanosensor immobilized with HBV-derived chimeric protein; and the use of the above nanosensor.
摘要翻译：提供乙型肝炎病毒（HBV）衣壳蛋白和葡萄球菌蛋白A（SPAB）B结构域的嵌合蛋白; 其制造方法; 固定有HBV衍生嵌合蛋白的底物和纳米传感器; 并使用上述纳米传感器。

5. 发明申请

US20110281300A1 PREPARATION METHOD OF RECOMBINANT PROTEIN BY USE OF A FUSION EXPRESSION PARTNER 审中-公开
标题翻译：通过使用融合表达伴侣的重组蛋白的制备方法
公开(公告)号：US20110281300A1
公开(公告)日：2011-11-17
申请号：US13108518
申请日：2011-05-16
申请人： Jeewon Lee , Kyung-Yeon Han , Jong-Am Song , Jin-Seung Park , Hyuk-Seong Seo , Keum-Young Ahn , Eun-Jung Lee , Jong-Hwan Lee , Soo-Jung Kwon
发明人： Jeewon Lee , Kyung-Yeon Han , Jong-Am Song , Jin-Seung Park , Hyuk-Seong Seo , Keum-Young Ahn , Eun-Jung Lee , Jong-Hwan Lee , Soo-Jung Kwon
IPC分类号： C12P21/00 , C12N15/63 , C12N1/21 , C12N9/00
CPC分类号： C12N15/67 , C12N15/64
摘要： The present invention relates to a preparation method using a fusion expression partner. The method includes preparing a polynucleotide encoding a fusion expression partner selected from the group consisting of SlyD (FKBR-type peptidyl prolyl cis-trans isomerase), Crr (glucose-specific phosphotransferase (PTS) enzyme IIA component), RpoS (RNA polymerase sigma factor), PotD (Spermidine/putrescine-binding periplasmic protein), and RpoA (RNA polymerase alpha subunit), and an expression vector linking a polyDNA fragment of a heterologous protein, preparing a transformant by introducing the expression vector into a host cell, inducing the expression of a recombinant protein by culturing a transformant, and obtaining the expression. In the preparation method of the recombinant protein, the heterologous protein may enhance the water-solubility and folding of the recombinant protein, overcome the limitations about the water-solubility and folding which the conventional fusion expression partners have, and be used widely in the production of pharmaceutical and industrial proteins.
摘要翻译：本发明涉及使用融合表达配偶体的制备方法。该方法包括制备编码融合表达配偶体的多核苷酸，其选自SlyD（FKBR型肽基脯氨酰顺反异构酶），Crr（葡萄糖特异性磷酸转移酶（PTS）酶IIA组分），RpoS（RNA聚合酶sigma因子），PotD（亚精胺/腐胺结合周质蛋白）和RpoA（RNA聚合酶α亚基），以及连接异源蛋白质的多聚DNA片段的表达载体，通过将表达载体导入宿主细胞来制备转化体，诱导通过培养转化体表达重组蛋白，获得表达。在重组蛋白的制备方法中，异源蛋白可增强重组蛋白的水溶性和折叠，克服了常规融合表达伴侣具有的水溶性和折叠的局限性，并广泛用于生产的药物和工业蛋白质。

6. 发明申请

US20130142732A1 RECOMBINANT FLUORESCENT NANOPARTICLES 审中-公开
标题翻译：重组荧光纳米颗粒
公开(公告)号：US20130142732A1
公开(公告)日：2013-06-06
申请号：US13487921
申请日：2012-06-04
申请人： Jee-Won Lee , Keum-Young Ahn , Jin-Seung Park
发明人： Jee-Won Lee , Keum-Young Ahn , Jin-Seung Park
IPC分类号： C07K19/00 , A61K49/00 , G01N21/64 , B82Y15/00
CPC分类号： A61K49/0047 , C07K14/43595 , C07K2319/735 , G01N2021/6439
摘要： A recombinant fluorescent protein nanoparticle having high fluorescence intensity and a method of detecting a target material using the same are provided. The protein nanoparticle has higher fluorescence intensity than a fluorescent protein, and is resistant to denaturation of the fluorescent protein at room temperature, thereby having higher structural stability than the fluorescent protein itself. In addition, since a self-assembled protein is used as a fusion partner of the fluorescent protein, the protein nanoparticle is biocompatible and safe. Moreover, when a linker peptide is additionally inserted into the protein nanoparticle, a suitable distance between the self-assembled protein and the fluorescent protein is maintained, thereby considerably increasing fluorescence intensity of the protein nanoparticle. The probe-binding protein nanoparticle can control distances between the fluorescent proteins on the surface thereof, thereby maximizing fluorescence intensity.
摘要翻译：提供具有高荧光强度的重组荧光蛋白纳米颗粒和使用其的目标材料的检测方法。蛋白质纳米颗粒具有比荧光蛋白更高的荧光强度，并且在室温下对荧光蛋白质的变性具有抗性，因此具有比荧光蛋白本身更高的结构稳定性。另外，由于使用自组装蛋白作为荧光蛋白的融合配偶体，所以蛋白质纳米颗粒是生物相容性和安全性的。此外，当连接肽另外插入到蛋白质纳米颗粒中时，维持自组装蛋白和荧光蛋白之间的适当距离，从而显着增加蛋白质纳米颗粒的荧光强度。探针结合蛋白纳米颗粒可以控制其表面上的荧光蛋白之间的距离，从而使荧光强度最大化。

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