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1. 发明授权

US6110671A Method of measuring tumor suppressor gene p53 失效
标题翻译：测量肿瘤抑制基因p53的方法
公开(公告)号：US6110671A
公开(公告)日：2000-08-29
申请号：US832502
申请日：1997-04-03
申请人： Jerome H. Kim
发明人： Jerome H. Kim
IPC分类号： C12Q1/68 , C07H21/04 , C12N15/10
CPC分类号： C12Q1/6809 , C12Q1/6886
摘要： The instant invention provides a means for quantitatively determining the vel of tumor suppressor gene p53 by determination of level of messenger ribonucleic acid (mRNA) of the gene in a sample when compared with a prepared standard. The assay is quantitative in that the specific number of copies of the p53 mRNA in a sample may be derived from a curve from a standard of p53 RNA. The RNA used in preparation of a standard curve to quantitate RNA is generated using a plasmid which is part of the invention. In the assay, the RNA is produced by a protein (RNA polymerase) that reads the DNA message and manufactures an RNA copy. The RNA content of the transcribed sample is determined spectrophotometrically to measure the molar concentration. With prior knowledge of the molecular weight of the transcribed RNA and using Avogadro's number, the actual number of molecules of transcripted "control" p53 RNA can be determined
摘要翻译：本发明提供了一种用于通过测定样品中的基因的信使核糖核酸（mRNA）的量与制备的标准物相比较来定量测定肿瘤抑制基因p53的水平的方法。该测定是定量的，因为样品中p53 mRNA的特异拷贝数可以来自p53RNA标准品的曲线。用于制备标准曲线以定量RNA的RNA是使用作为本发明一部分的质粒产生的。在测定中，RNA由读取DNA消息并制造RNA拷贝的蛋白质（RNA聚合酶）产生。通过分光光度法测定转录样品的RNA含量以测量摩尔浓度。利用转录RNA的分子量和使用阿伏伽德罗数的先前知识，可以确定转录的“对照”p53 RNA的实际分子数

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