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1. 发明授权

US07202054B2 DNA molecules encoding L-glutamate-gated chloride channels from Rhipicephalus sanguineus 有权
标题翻译：从Rhipicephalus sanguineus编码L-谷氨酸氯化物通道的DNA分子
公开(公告)号：US07202054B2
公开(公告)日：2007-04-10
申请号：US10239588
申请日：2001-03-28
申请人： Jeffrey W. Warmke , Youfeng Yang , Doris F. Cully , Michel J. Hamelin
发明人： Jeffrey W. Warmke , Youfeng Yang , Doris F. Cully , Michel J. Hamelin
IPC分类号： C12P21/02 , C12N5/16 , C12N1/21 , C12N15/63 , C07H21/04
CPC分类号： C07K14/43527 , C07K14/705 , G01N33/6872 , G01N2333/43556
摘要： The present invention relates in part to isolated nucleic acid molecules (polynucleotides) which encode Rhipicephalus sanguineus glutamate gated chloride channels. The present invention also relates to recombinant vectors and recombinant hosts which contain a DNA fragment encoding R. sanguineus glutamate gated chloride channels, substantially purified forms of associated R. sanguineus glutamate gated chloride channels and recombinant membrane fractions comprising these proteins, associated mutant proteins, and methods associated with identifying compounds which modulate associated Rhipicephalus sanguineus glutamate gated chloride channels, which will be useful as insecticides.
摘要翻译：本发明部分涉及编码Rigicephalus sanguineus glutamate门控氯化物通道的分离的核酸分子（多核苷酸）。本发明还涉及重组载体和重组宿主，其含有编码雷神酵母谷氨酸盐门控氯化物通道的DNA片段，基本上纯化形式的相关的谷氨酸谷氨酸盐化氯化物通道和包含这些蛋白质的重组膜级分，相关突变蛋白质，和与鉴定调节相关联的Rhipicephalus sanguineus glutamate门控氯化物通道的化合物相关的方法，其将用作杀虫剂。

2. 发明申请

US20080286770A1 DNA Molecules Encoding L-Glutamate-Gated Chloride Channels From Rhipicephalus Sanguineus 有权
公开(公告)号：US20080286770A1
公开(公告)日：2008-11-20
申请号：US11620390
申请日：2007-01-05
申请人： Jeffrey W. Warmke , Youfeng Yang , Doris F. Cully , Michel J. Hamelin
发明人： Jeffrey W. Warmke , Youfeng Yang , Doris F. Cully , Michel J. Hamelin
IPC分类号： C12Q1/68 , G01N33/00
CPC分类号： C07K14/43527 , C07K14/705 , G01N33/6872 , G01N2333/43556
摘要： The present invention relates in part to isolated nucleic acid molecules (polynucleotides) which encode Rhipicephalus sanguineus glutamate gated chloride channels. The present invention also relates to recombinant vectors and recombinant hosts which contain a DNA fragment encoding R. sanguineus glutamate gated chloride channels, substantially purified forms of associated R. sanguineus glutamate gated chloride channels and recombinant membrane fractions comprising these proteins, associated mutant proteins, and methods associated with identifying compounds which modulate associated Rhipicephalus sanguineus glutamate gated chloride channels, which will be useful as insecticides.

3. 发明授权

US07001734B1 Process for identifying para cation channel modulators 失效
标题翻译：识别对位通道调节剂的方法
公开(公告)号：US07001734B1
公开(公告)日：2006-02-21
申请号：US08554424
申请日：1995-11-06
申请人： Leonardus H. T. Van Der Ploeg , Jeffrey W. Warmke
发明人： Leonardus H. T. Van Der Ploeg , Jeffrey W. Warmke
IPC分类号： G01N33/567
CPC分类号： C07K16/18 , A61K38/00 , C07K14/435 , C12N2799/026 , G01N33/5014 , G01N33/6872 , G01N2500/00
摘要： DNAs encoding voltage-activated cation channels have been cloned and characterized. The cDNA's have been expressed in recombinant host cells which produce active recombinant protein. The recombinant protein is also purified from the recombinant host cells. In addition, the recombinant host cells are utilized to establish a method for identifying modulators of the channel activity, and channel modulators are identified. Channel modulators are useful as insecticides and arachnicidic agents.
摘要翻译：编码电压激活阳离子通道的DNA已被克隆和表征。 cDNA已经在产生活性重组蛋白的重组宿主细胞中表达。重组蛋白也从重组宿主细胞中纯化。另外，重组宿主细胞用于建立识别通道活性的调节剂的方法，并鉴定通道调节剂。通道调节剂可用作杀虫剂和花生四烯酸酯。

4. 发明授权

US07915010B1 DNA molecules encoding L-glutamate-gated chloride channels from Rhipicephalus sanguineus 有权
标题翻译：从Rhipicephalus sanguineus编码L-谷氨酸氯化物通道的DNA分子
公开(公告)号：US07915010B1
公开(公告)日：2011-03-29
申请号：US11620435
申请日：2007-01-05
申请人： Jeffrey W. Warmke , Youfeng Yang , Doris F. Cully , Michel J. Hamelin
发明人： Jeffrey W. Warmke , Youfeng Yang , Doris F. Cully , Michel J. Hamelin
IPC分类号： C12P21/02 , C12N5/16 , C12N1/21 , C12N15/63 , C07H21/04
CPC分类号： C07K14/43527 , C07K14/705 , G01N33/6872 , G01N2333/43556
摘要： The present invention relates in part to isolated nucleic acid molecules (polynucleotides) which encode Rhipicephalus sanguineus glutamate gated chloride channels. The present invention also relates to recombinant vectors and recombinant hosts which contain a DNA fragment encoding R. sanguineus glutamate gated chloride channels, substantially purified forms of associated R. sanguineus glutamate gated chloride channels and recombinant membrane fractions comprising these proteins, associated mutant proteins, and methods associated with identifying compounds which modulate associated Rhipicephalus sanguineus glutamate gated chloride channels, which will be useful as insecticides.
摘要翻译：本发明部分涉及编码Rigicephalus sanguineus glutamate门控氯化物通道的分离的核酸分子（多核苷酸）。本发明还涉及重组载体和重组宿主，其含有编码雷神酵母谷氨酸盐门控氯化物通道的DNA片段，基本上纯化形式的相关的谷氨酸谷氨酸盐化氯化物通道和包含这些蛋白质的重组膜级分，相关突变蛋白质，和与鉴定调节相关联的Rhipicephalus sanguineus glutamate门控氯化物通道的化合物相关的方法，其将用作杀虫剂。

5. 发明授权

US07033789B1 DNA molecules encoding L-glutamate-gated chloride channels from Schistocerca americana 失效
标题翻译：编码来自美国黑曲霉的L-谷氨酸盐化氯化物通道的DNA分子
公开(公告)号：US07033789B1
公开(公告)日：2006-04-25
申请号：US10111161
申请日：2000-10-19
申请人： Michel J. Hamelin , Jeffrey W. Warmke
发明人： Michel J. Hamelin , Jeffrey W. Warmke
IPC分类号： C07K14/705 , C12N5/10 , C12N15/12 , C12N15/63
CPC分类号： C07K14/705
摘要： The present invention relates in part to isolated nucleic acid molecules (polynucleotides) which encode Schistocerca americana (grasshopper) glutamate-gated chloride channels. The present invention also relates to recombinant vectors and recombinant hosts which contain a DNA fragment encoding S. americana glutamate-gated chloride channels, substantially purified forms of associated S. americana glutamate-gated chloride channels and recombinant membrane fractions comprising these proteins, associated mutant proteins, and methods associated with identifying compounds which modulate associated Schistocerca americana glutamate-gated chloride channels, which will be useful as insecticides.
摘要翻译：本发明部分涉及编码美国Sch）（蚱蜢）谷氨酸盐化氯通道的分离的核酸分子（多核苷酸）。本发明还涉及重组载体和重组宿主，其含有编码美国谷氨酸盐门控氯化物通道的DNA片段，基本纯化形式的相关美国谷氨酸盐门控氯化物通道和包含这些蛋白质的重组膜级分，相关突变蛋白以及与鉴定调节美国相关美国谷氨酸盐门控氯化物通道的化合物相关的方法，其可用作杀虫剂。

6. 发明授权

US06358701B1 DNA molecules encoding Ctenocephalides felis glutamate gated chloride channels 失效
标题翻译：编码Ctenocephalides felis glutamate门控氯化物通道的DNA分子
公开(公告)号：US06358701B1
公开(公告)日：2002-03-19
申请号：US09130339
申请日：1998-08-06
申请人： Jeffrey W. Warmke , Doris F. Cully , Adrian Etter , Philip S. Paress , Charles J. Cohen , Richard Brochu
发明人： Jeffrey W. Warmke , Doris F. Cully , Adrian Etter , Philip S. Paress , Charles J. Cohen , Richard Brochu
IPC分类号： C07H2104
CPC分类号： C07K14/4359 , C07K14/70571
摘要： To date, L-glutamate-gated chloride (GluCl) channels have been observed only in invertebrate organisms. Modulators of this channel (either agonists or antagoinists) will interfere with neurotransmission. For example, agents such as avermectins activate the GluCl, causing paralysis due to blocking of neurotranmitter release, resulting in death of the organism. Because GluCl channels are invertebreate specific, they are excellent targets for the discovery of novel insecticides, anthelminths and parasiticides that will display a marked safety profile because of the lack of mechanism based toxicity in vertebrate organisms. The present specification discloses isolation of a cDNA clone from the cat flea Ctenocephalides felis (CfGluCl-1) that encodes a L-glutamate-gated chloride channel. Heterologous expression of CfGluCl-1 cRNA in Xenopus oocytes results in robust expression of a L-glutamate-gated chloride current and the channel is activated and potentiated by avermectins. The expression of CfGluCl-1 in a heterologous expression system if useful to screens for novel GluCl channel agonsts and antagonsits. Additionally, this specification disclose impoved methods of screening for GluCl channel modulators.
摘要翻译：迄今为止，仅在无脊椎动物体内观察到L-谷氨酸氯化物（GluCl）通道。该通道的调节剂（激动剂或antagoinists）将干扰神经传递。例如，除虫菌素之类的试剂激活GluCl，由于神经递质释放的阻断导致麻痹，导致生物体的死亡。由于GluCl通道具有特异性，因此它们是发现新型杀虫剂，anthelminths和杀寄生虫剂的优秀目标，由于脊椎动物体内缺乏基于机制的毒性，因此将显示出明显的安全性。本说明书公开了从编码L-谷氨酸盐化氯化物通道的猫蚤Ctenocephalides felis（CfGluCl-1）中分离cDNA克隆。非洲爪蟾卵母细胞中CfGluCl-1 cRNA的异源表达导致L-谷氨酸氯化物电流的强烈表达，并且通道被阿凡曼菌素激活和增强。 CfGluCl-1在异源表达系统中的表达如果可用于筛选新的GluCl通道激动剂和antagonsits。另外，本说明书公开了筛选GluCl通道调节剂的方法。

7. 发明授权

US07541432B2 L-glutamate-gated chloride channel proteins from Rhipicephalus sanguineus 有权
标题翻译：来自Rhipicephalus sanguineus的L-谷氨酸氯化物通道蛋白
公开(公告)号：US07541432B2
公开(公告)日：2009-06-02
申请号：US11620347
申请日：2007-01-05
申请人： Jeffrey W. Warmke , Youfeng Yang , Doris F. Cully , Michel J. Hamelin
发明人： Jeffrey W. Warmke , Youfeng Yang , Doris F. Cully , Michel J. Hamelin
IPC分类号： C07K14/00 , C12N15/63 , C12N5/16 , C12N1/19
CPC分类号： C07K14/43527 , C07K14/705 , G01N33/6872 , G01N2333/43556
摘要： The present invention relates in part to isolated nucleic acid molecules (polynucleotides) which encode Rhipicephalus sanguineus glutamate gated chloride channels. The present invention also relates to recombinant vectors and recombinant hosts which contain a DNA fragment encoding R. sanguineus glutamate gated chloride channels, substantially purified forms of associated R. sanguineus glutamate gated chloride channels and recombinant membrane fractions comprising these proteins, associated mutant proteins, and methods associated with identifying compounds which modulate associated Rhipicephalus sanguineus glutamate gated chloride channels, which will be useful as insecticides.
摘要翻译：本发明部分涉及编码Rigicephalus sanguineus glutamate门控氯化物通道的分离的核酸分子（多核苷酸）。本发明还涉及重组载体和重组宿主，其含有编码雷神酵母谷氨酸盐门控氯化物通道的DNA片段，基本上纯化形式的相关的谷氨酸谷氨酸盐化氯化物通道和包含这些蛋白质的重组膜级分，相关突变蛋白质，和与鉴定调节相关联的Rhipicephalus sanguineus glutamate门控氯化物通道的化合物相关的方法，其将用作杀虫剂。

8. 发明授权

US5688917A Process for functional expression of the para cation channel 失效
标题翻译：对位阳离子通道的功能表达过程
公开(公告)号：US5688917A
公开(公告)日：1997-11-18
申请号：US724095
申请日：1996-09-30
申请人： Joseph P. Arena , Guoping Feng , Linda M. Hall , Ken Liu , Leonardus H. T. Van Der Ploeg , Peiyi Wang , Jeffrey W. Warmke
发明人： Joseph P. Arena , Guoping Feng , Linda M. Hall , Ken Liu , Leonardus H. T. Van Der Ploeg , Peiyi Wang , Jeffrey W. Warmke
IPC分类号： C12N15/09 , C07K14/705 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12P21/02 , C12R1/19 , C12R1/865 , C12R1/91 , C07K1/00 , C07K14/00 , C12P21/06 , C12Q1/00
CPC分类号： C07K14/705 , Y10T428/2984
摘要： DNAs encoding voltage-activated cation channels have been cloned and characterized. The cDNA's have been expressed in recombinant host cells which produce active recombinant protein. The recombinant protein is also purified from the recombinant host cells.
摘要翻译：编码电压激活阳离子通道的DNA已被克隆和表征。 cDNA已经在产生活性重组蛋白的重组宿主细胞中表达。重组蛋白也从重组宿主细胞纯化。

9. 发明授权

US07674586B2 Methods of identifying modulators of L-glutamate-gated chloride channel proteins from Rhipicephalus sanguineus 有权
标题翻译：鉴定来自R us属的L-谷氨酸盐化氯化物通道蛋白的调节剂的方法
公开(公告)号：US07674586B2
公开(公告)日：2010-03-09
申请号：US11620390
申请日：2007-01-05
申请人： Jeffrey W. Warmke , Youfeng Yang , Doris F. Cully , Michel J. Hamelin
发明人： Jeffrey W. Warmke , Youfeng Yang , Doris F. Cully , Michel J. Hamelin
IPC分类号： C12Q1/68 , C12Q1/00
CPC分类号： C07K14/43527 , C07K14/705 , G01N33/6872 , G01N2333/43556
摘要： The present invention relates in part to isolated nucleic acid molecules (polynucleotides) which encode Rhipicephalus sanguineus glutamate gated chloride channels. The present invention also relates to recombinant vectors and recombinant hosts which contain a DNA fragment encoding R. sanguineus glutamate gated chloride channels, substantially purified forms of associated R. sanguineus glutamate gated chloride channels and recombinant membrane fractions comprising these proteins, associated mutant proteins, and methods associated with identifying compounds which modulate associated Rhipicephalus sanguineus glutamate gated chloride channels, which will be useful as insecticides.
摘要翻译：本发明部分涉及编码Rigicephalus sanguineus glutamate门控氯化物通道的分离的核酸分子（多核苷酸）。本发明还涉及重组载体和重组宿主，其含有编码雷神酵母谷氨酸盐门控氯化物通道的DNA片段，基本上纯化形式的相关的谷氨酸谷氨酸盐化氯化物通道和包含这些蛋白质的重组膜级分，相关突变蛋白质，和与鉴定调节相关联的Rhipicephalus sanguineus glutamate门控氯化物通道的化合物相关的方法，其将用作杀虫剂。

10. 发明授权

US5593864A Process for functional expression of the para cation channel 失效
公开(公告)号：US5593864A
公开(公告)日：1997-01-14
申请号：US337339
申请日：1994-11-10
申请人： Joseph P. Arena , Guoping Feng , Linda M. Hall , Ken Liu , Leonardus H. T. Van Der Ploeg , Peiyi Wang , Jeffrey W. Warmke
发明人： Joseph P. Arena , Guoping Feng , Linda M. Hall , Ken Liu , Leonardus H. T. Van Der Ploeg , Peiyi Wang , Jeffrey W. Warmke
IPC分类号： C12N15/09 , C07K14/705 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12P21/02 , C12R1/19 , C12R1/865 , C12R1/91 , C12P21/06 , C12N5/00
CPC分类号： C07K14/705 , Y10T428/2984
摘要： DNAs encoding voltage-activated cation channels have been cloned and characterized. The cDNA's have been expressed in recombinant host cells which produce active recombinant protein. The recombinant protein is also purified from the recombinant host cells.

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