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1. 发明申请

WO2005124351A8 A SENSITIVE ANTIBODY-BASED METHOD FOR DETECTING CRYPTOSPORIDIUM PARVUM OOCYSTS IN WATER 审中-公开
标题翻译：用于检测水中碳酸氢钠水溶性的敏感抗体方法
公开(公告)号：WO2005124351A8
公开(公告)日：2006-12-21
申请号：PCT/US2005018936
申请日：2005-05-31
申请人： US AGRICULTURE , JENKINS MARK C , KNIEL KALMIA K
发明人： JENKINS MARK C , KNIEL KALMIA K
IPC分类号： A61K39/00 , A61K39/002 , C12Q1/00 , G01N33/53 , G01N33/569
CPC分类号： G01N33/56983
摘要： A viral symbiont (CPV) of Cryptosporidium parvum and Cryptosporidium hominis sporozoites has been characterized and a CPV capsid protein, CPV40, has been identified as a target for sensitive detection of C. parvum. Recombinant CPV40 was produced in E. coli, purified by affinity chromatography, and used to prepare polyclonal rabbit sera specific for the viral capsid protein. Anti-rCPV40 recognized a 40 kDa and a 30 kDa protein in C. parvum oocysts and appeared to localize to the apical end of the parasite. Anti-rCPV40 serum was capable of detecting as few as one C. parvum or C.hominis oocyst in a dot blot assay, the sensitivity being at least 1000-fold greater than sera reactive with total native C. parvum protein or specific for the 41 kDa C. parvum oocyst surface antigen. Water samples were seeded with C parvum
摘要翻译：已经表征了隐孢子虫和隐孢子虫子孢子的病毒共生体（CPV），并且CPV衣壳蛋白CPV40已被鉴定为敏感检测小球茎的靶标。重组CPV40在大肠杆菌中产生，通过亲和层析纯化，并用于制备特异于病毒衣壳蛋白的多克隆兔血清。抗rCPV40在小叶卵囊中识别出40kDa和30kDa的蛋白质，并且似乎定位于寄生虫的顶端。抗rCPV40血清在斑点印迹测定中能够检测到少至1个小时或C.hominis卵囊，其敏感性比对总天然小球蛋白具有反应性的血清至少高1000倍或特异于41 小麦卵母细胞表面抗原。水样用小白菜种子

2. 发明申请

WO2009105355A3 A NOVEL NEOSPORA CANINUM VACCINE 审中-公开
标题翻译：一个新的NEOSPORA CANINUM VACCINE
公开(公告)号：WO2009105355A3
公开(公告)日：2009-10-22
申请号：PCT/US2009033437
申请日：2009-02-06
申请人： US AGRICULTURE , TUO WENBIN , JENKINS MARK C , ZHAO YAN
发明人： TUO WENBIN , JENKINS MARK C , ZHAO YAN
IPC分类号： A61K39/002 , A61K48/00 , A61P33/02 , A61P37/04
CPC分类号： A61K39/002 , A61K2039/545 , A61K2039/552 , A61K2039/55561 , A61K2039/55566
摘要： Neospora caninum is the causal agent of bovine neosporosis which results in high levels of abortion. The present study determined the protective efficacy of two Neospora antigens- Neospora cyclophilin (NcCyP) and NcSRS2. The ability of native NcCyP to upregulate mouse IFN? was also confirmed in this study. Recombinant NcCyP or NcSRS2 were tested either alone or in combination and formulated with adjuvant ImmuMax-SR and CpG. Female BALB/c mice (n=15) of 10-12 weeks of age were immunized s.c. twice in a 2-week interval with vaccines containing either NcCyP alone, NcSRS2 alone, NcCyP plus NcSRS2, or non-recombinant bacterial antigen (NR) in 2 separate trials. All mice were challenge-infected 3 weeks following the booster immunization and necropsied 3 weeks after the challenge infection. Brain and serum were collected and Nc-specific DNA sequence in brain tissue and antibodies in serum were analyzed by PCR or ELISA/Westem blotting. Results showed that mice vaccinated with rNcCyP, rNcSRS2, or both rNcCyP and rNcSRS2 responded with high levels of NcCyP or NcSRS2 specific antibodies. Overall, mice received vaccines formulated with either rNcCyP or rNcCyP and rNcSRS2 had a higher (p
摘要翻译：新孢子虫是牛新孢子虫病的致病因子，导致高水平的流产。本研究确定了两种新孢子虫抗原 - 新孢子虫亲环蛋白（NcCyP）和NcSRS2的保护功效。天然NcCyP上调小鼠IFN的能力在本研究中也得到证实。单独或组合测试重组NcCyP或NcSRS2，并用佐剂ImmuMax-SR和CpG配制。 10-12周龄的雌性BALB / c小鼠（n = 15）免疫s.c. 在两个单独的试验中，在含有单独的NcCyP，单独的NcSRS2，NcCyP加NcSRS2或非重组细菌抗原（NR）的疫苗的两周间隔中两次。所有小鼠在加强免疫后3周进行攻击感染，并在攻击感染后3周进行尸检。收集脑和血清，通过PCR或ELISA / Western印迹分析血清中脑组织和抗体中的Nc特异性DNA序列。结果表明，用rNcCyP，rNcSRS2或rNcCyP和rNcSRS2两者接种的小鼠均响应高水平的NcCyP或NcSRS2特异性抗体。总体而言，小鼠接受用rNcCyP或rNcCyP配制的疫苗，当与模拟或未接种疫苗的小鼠相比时，rNcSRS2具有较高的（p <0.01）的保护。单独使用rNcSRS2免疫的组表现出略低的保护水平，比未接种组更高（p <0.05），但与模拟接种组相比没有差异（p = 0.06）。本研究的结果表明，NcCyP是一种非常有效的候选疫苗，可用于防止新孢子虫感染。

3. 发明申请

WO2005124351A3 A SENSITIVE ANTIBODY-BASED METHOD FOR DETECTING CRYPTOSPORIDIUM PARVUM OOCYSTS IN WATER 审中-公开
标题翻译：一种灵敏的基于抗体的检测水中隐孢子虫卵囊的方法
公开(公告)号：WO2005124351A3
公开(公告)日：2006-04-13
申请号：PCT/US2005018936
申请日：2005-05-31
申请人： US AGRICULTURE , JENKINS MARK C , KNIEL KALMIA K
发明人： JENKINS MARK C , KNIEL KALMIA K
IPC分类号： G01N33/569 , A61K39/00 , A61K39/02 , C12Q1/00 , G01N33/53
CPC分类号： G01N33/56983
摘要： A viral symbiont (CPV) of Cryptosporidium parvum and Cryptosporidium hominis sporozoites has been characterized and a CPV capsid protein, CPV40, has been identified as a target for sensitive detection of C. parvum. Recombinant CPV40 was produced in E. coli, purified by affinity chromatography, and used to prepare polyclonal rabbit sera specific for the viral capsid protein. Anti-rCPV40 recognized a 40 kDa and a 30 kDa protein in C. parvum oocysts and appeared to localize to the apical end of the parasite. Anti-rCPV40 serum was capable of detecting as few as one C. parvum or C.hominis oocyst in a dot blot assay, the sensitivity being at least 1000-fold greater than sera reactive with total native C. parvum protein or specific for the 41 kDa C. parvum oocyst surface antigen. Water samples were seeded with C parvum
摘要翻译： Cryptosporidium parvum和Cryptosporidium hominis子孢子的病毒共生体（CPV）已经被鉴定，并且CPV衣壳蛋白CPV40已经被确定为敏感检测小隐孢子虫的目标。在大肠杆菌中产生重组CPV40，通过亲和层析纯化，并用于制备病毒衣壳蛋白特异性的多克隆兔血清。抗rCPV40在C. parvum卵囊中识别40kDa和30kDa蛋白质，并且似乎定位于寄生虫的顶端。抗rCPV40血清能够在斑点印迹测定中检测少至一个小隐孢子虫或人单胞菌卵囊，其灵敏度比总天然小隐孢子虫蛋白质反应的血清高至少1000倍或对41 kDa C. parvum卵囊表面抗原。水样采用C parvum

4. 发明申请

WO2005124351A2 A SENSITIVE ANTIBODY-BASED METHOD FOR DETECTING CRYPTOSPORIDIUM PARVUM OOCYSTS IN WATER 审中-公开
标题翻译：用于检测水中碳酸氢钠水溶性的敏感抗体方法
公开(公告)号：WO2005124351A2
公开(公告)日：2005-12-29
申请号：PCT/US2005/018936
申请日：2005-05-31
申请人： THE UNITED STATES OF AMERICA, as represented by THE SECRETARY OF AGRICULTURE , JENKINS, Mark, C. , KNIEL, Kalmia, K.
发明人： JENKINS, Mark, C. , KNIEL, Kalmia, K.
IPC分类号： G01N33/569
CPC分类号： G01N33/56983
摘要： A viral symbiont (CPV) of Cryptosporidium parvum and Cryptosporidium hominis sporozoites has been characterized and a CPV capsid protein, CPV40, has been identified as a target for sensitive detection of C . parvum. Recombinant CPV40 was produced in E . coli, purified by affinity chromatography, and used to prepare polyclonal rabbit sera specific for the viral capsid protein. Anti-rCPV40 recognized a 40 kDa and a 30 kDa protein in C. parvum oocysts and appeared to localize to the apical end of the parasite. Anti-rCPV40 serum was capable of detecting as few as one C. parvum or C . hominis oocyst in a dot blot assay, the sensitivity being at least 1000-fold greater than sera reactive with total native C. parvum protein or specific for the 41 kDa C. parvum oocyst surface antigen. Water samples were seeded with C parvum oocysts and incubated at 4, 20, or 25 °C for greater than 3 months to determine if CPV levels were correlated with oocyst infectivity and viral particle presence. While sporozoite infectivity declined by more than 75% after 1 month at 25 °C, the CPV signal was similar to that of control samples at 4 ° C. By three months at 20 °C, the C . parvum oocysts were found to be non-infectious, but retained a high CPV signal. CPV is an excellent target for sensitive detection of C . parvum and C . hominis oocysts in water, but may persist for an indefinite time after oocysts become non-infectious.
摘要翻译：已经表征了隐孢子虫和隐孢子虫子孢子的病毒共生体（CPV），并且CPV衣壳蛋白CPV40已被鉴定为敏感检测小球茎的靶标。重组CPV40在大肠杆菌中产生，通过亲和层析纯化，并用于制备特异于病毒衣壳蛋白的多克隆兔血清。抗rCPV40在小叶卵囊中识别出40kDa和30kDa的蛋白质，并且似乎定位于寄生虫的顶端。抗rCPV40血清在斑点印迹测定中能够检测少至1个小时或人血清卵囊，其敏感性比对总天然小球蛋白反应的血清至少高1000倍，或者特异于41 小麦卵母细胞表面抗原。将水样品接种小孢子虫卵囊，并在4,20或25℃下孵育3个月以上，以确定CPV水平是否与卵囊传染性和病毒颗粒存在相关。虽然子孢子传染性在25℃下1个月后下降了75％以上，CPV信号与对照样品在4℃相似。在20°C三个月后，小孢子虫卵囊被发现是非传染性的，但保留了较高的CPV信号。 CPV是敏感检测水中小球虫和人类血红蛋白的优良目标，但卵囊变得不感染后可能会持续无限期。

5. 发明申请

WO2013012683A3 GEL VACCINE DELIVERY SYSTEM FOR TREATING POULTRY 审中-公开
标题翻译：用于治疗流感的凝胶疫苗输送系统
公开(公告)号：WO2013012683A3
公开(公告)日：2013-04-04
申请号：PCT/US2012046493
申请日：2012-07-12
申请人： US AGRICULTURE , SOUTHWEST RES INST , JENKINS MARK C , FETTERER RAYMOND H , PERSYN JOSEPH T
发明人： JENKINS MARK C , FETTERER RAYMOND H , PERSYN JOSEPH T
IPC分类号： A61K47/48 , A61K39/02 , A61K39/12 , A61K47/30 , A61K47/38
CPC分类号： A61K39/002 , A61K9/0056 , A61K9/1658 , A61K39/012 , A61K2039/54 , A61K2039/542
摘要： Safe and effective gel-bead vaccines for treating domesticated birds for diseases caused by cyst-forming protozoa, especially for coccidiosis.
摘要翻译：安全有效的凝胶珠疫苗用于治疗家禽由于囊肿形成原生动物引起的疾病，特别是针对球虫病。

6. 发明申请

WO2009105355A2 A NOVEL NEOSPORA CANINUM VACCINE 审中-公开
标题翻译：一种新型NEOSPORA CANINUM疫苗
公开(公告)号：WO2009105355A2
公开(公告)日：2009-08-27
申请号：PCT/US2009/033437
申请日：2009-02-06
申请人： THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF AGRICULTURE , TUO, Wenbin , JENKINS, Mark, C. , ZHAO, Yan
发明人： TUO, Wenbin , JENKINS, Mark, C. , ZHAO, Yan
IPC分类号： A61K39/002 , A61K48/00 , A61P37/04 , A61P33/02
CPC分类号： A61K39/002 , A61K2039/545 , A61K2039/552 , A61K2039/55561 , A61K2039/55566
摘要： Neospora caninum is the causal agent of bovine neosporosis which results in high levels of abortion. The present study determined the protective efficacy of two Neospora antigens- Neospora cyclophilin (NcCyP) and NcSRS2. The ability of native NcCyP to upregulate mouse IFNγ was also confirmed in this study. Recombinant NcCyP or NcSRS2 were tested either alone or in combination and formulated with adjuvant ImmuMax-SR and CpG. Female BALB/c mice (n=15) of 10-12 weeks of age were immunized s.c. twice in a 2-week interval with vaccines containing either NcCyP alone, NcSRS2 alone, NcCyP plus NcSRS2, or non-recombinant bacterial antigen (NR) in 2 separate trials. All mice were challenge-infected 3 weeks following the booster immunization and necropsied 3 weeks after the challenge infection. Brain and serum were collected and Nc-specific DNA sequence in brain tissue and antibodies in serum were analyzed by PCR or ELISA/Westem blotting. Results showed that mice vaccinated with rNcCyP, rNcSRS2, or both rNcCyP and rNcSRS2 responded with high levels of NcCyP or NcSRS2 specific antibodies. Overall, mice received vaccines formulated with either rNcCyP or rNcCyP and rNcSRS2 had a higher (p
摘要翻译：犬新孢子虫是牛新孢子虫病的致病因子，其导致高水平的流产。本研究确定了两种新孢子虫抗原 - 新孢子虫亲环蛋白（NcCyP）和NcSRS2的保护效力。本研究也证实了天然NcCyP上调小鼠IFNγ的能力。单独或联合测试重组NcCyP或NcSRS2，并用佐剂ImmuMax-SR和CpG配制。 10〜12周龄的雌性BALB / c小鼠（n = 15）在2周的时间间隔内用含有单独的NcCyP，单独的NcSRS2，NcCyP加NcSRS2或非重组细菌抗原（NR）的疫苗在两个单独的试验中两次。所有小鼠在加强免疫后3周被攻击感染并且在攻击感染后3周进行尸体解剖。收集脑和血清，通过PCR或ELISA / Westem印迹分析脑组织中的Nc特异性DNA序列和血清中的抗体。结果显示用rNcCyP，rNcSRS2或rNcCyP和rNcSRS2二者接种的小鼠以高水平的NcCyP或NcSRS2特异性抗体应答。总体而言，小鼠接受与rNcCyP或rNcCyP配制的疫苗，并且与模拟或未接种疫苗的小鼠相比，rNcSRS2具有更高的（p <0.01）百分比保护。单独用rNcSRS2免疫的组表现出稍低的保护水平，其比未接种疫苗组的保护水平稍高（p <0.05），但与模拟疫苗接种组相比没有差异（p = 0.06）。本研究结果表明，NcCyP是一种高效的候选疫苗，可用于防治新孢子虫感染。

7. 发明申请

WO2013012683A2 GEL VACCINE DELIVERY SYSTEM FOR TREATING POULTRY 审中-公开
标题翻译：用于治疗家禽的GEL疫苗递送系统
公开(公告)号：WO2013012683A2
公开(公告)日：2013-01-24
申请号：PCT/US2012/046493
申请日：2012-07-12
申请人： THE UNITED STATES OF AMERICA, as represented by THE SECRETARY OF AGRICULTURE , SOUTHWEST RESEARCH INSTITUTE , JENKINS, Mark C. , FETTERER, Raymond H. , PERSYN, Joseph T.
发明人： JENKINS, Mark C. , FETTERER, Raymond H. , PERSYN, Joseph T.
IPC分类号： A61K47/48 , A61K47/30 , A61K47/38 , A61K39/02 , A61K39/12
CPC分类号： A61K39/002 , A61K9/0056 , A61K9/1658 , A61K39/012 , A61K2039/54 , A61K2039/542
摘要： Safe and effective gel-bead vaccines for treating domesticated birds for diseases caused by cyst-forming protozoa, especially for coccidiosis.
摘要翻译：安全有效的凝胶珠疫苗，用于治疗家禽因囊肿原虫引起的疾病，尤其是球虫病。

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