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2. 发明申请

WO2003031592A2 GENE MARKERS FOR BEEF MARBLING AND TENDERNESS 审中-公开
标题翻译：基因标记用于牛肉马克隆和时间
公开(公告)号：WO2003031592A2
公开(公告)日：2003-04-17
申请号：PCT/US2002/032631
申请日：2002-10-11
申请人： THE OHIO STATE UNIVERSITY RESEARCH FOUNDATION , JACKWOOD, Daral, J. , FLUHARTY, Francis
发明人： JACKWOOD, Daral, J. , FLUHARTY, Francis
IPC分类号： C12N
CPC分类号： C12Q1/6888 , Y10S435/912
摘要： Methods and compositions are provided for objectively identifying: i) bovine animals having the genetic potential to produce beef that is marbled or tender, and ii) bovine carcasses whose beef is marbled or tender. The methods comprise extracting DNA from a sample obtained from a bovine animal or carcass, assaying for the presence of a DNA comprising a sequence, referred to hereinafter as a "genetic marker", in the DNA sample. In one aspecet, the genetic marker is a marker of marbling, and comprises the sequence set forth in SEQ ID NO. 1. In another aspect, the genetic marker is a marker of tenderness, and comprises the sequence set forth in SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 or combinations thereof. The compositions include primers that ammplify markers of marbled or tender beef present in bovine animal or carcass genomes and hybridization probes to detect marbling or tenderness markers.
摘要翻译：提供了用于客观鉴定的方法和组合物：i）具有产生大理石化或嫩化的牛肉的遗传潜力的牛动物，以及ii）其牛肉具有大理石纹或嫩度的牛胴体。该方法包括从获自牛动物或屠体的样品中提取DNA，测定DNA样品中是否存在包含以下称为“遗传标记”的序列的DNA。在一个aspecet中，所述遗传标记是大理石花纹的标记，并且包含SEQ ID NO.1中给出的序列。在另一方面，遗传标记是嫩度的标记，并且包含SEQ ID NO.1中给出的序列。 2，SEQ ID NO。 3，SEQ ID NO。 4或其组合。该组合物包括引发存在于牛动物或屠体基因组中的大理石或嫩牛肉的标志物和用于检测大理石纹或嫩度标志物的杂交探针的引物。

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