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1. 发明授权

US07479377B2 Genetic construct intracellular monitoring system 有权
标题翻译：遗传构建细胞内监测系统
公开(公告)号：US07479377B2
公开(公告)日：2009-01-20
申请号：US10229747
申请日：2002-08-27
申请人： Sharon Zhao , Inna Vainshtein , Richard M. Eglen
发明人： Sharon Zhao , Inna Vainshtein , Richard M. Eglen
IPC分类号： G12Q1/68 , G01N33/53 , G01N33/58 , C12N15/00 , C12N5/10 , C12N5/16
CPC分类号： C40B30/04 , C07K2319/60 , C07K2319/80 , C12N15/62 , C12Q1/34 , G01N33/5005 , G01N33/542 , G01N33/6845 , G01N2333/924 , G01N2500/20 , G01N2800/52
摘要： A system is provided an enzyme donor (“ED”) fused a surrogate of a mammalian protein of interest, where the fusion protein has the function of the natural protein. A vector is provided comprising a regulatory region functional in a mammalian host cell, a sequence encoding the ED joined to a multiple cloning site, an enzyme acceptor (“EA”) protein or enzyme acceptor sequence encoding such protein, and substrate for the enzyme formed by ED and EA.
摘要翻译：提供一种系统，其融合了目标哺乳动物蛋白质的替代物，其中融合蛋白具有天然蛋白质的功能的酶供体（“ED”）。提供载体，其包含哺乳动物宿主细胞中功能的调节区，编码与多克隆位点连接的ED的序列，编码该蛋白的酶受体（“EA”）蛋白或酶受体序列，以及形成的酶的底物由ED和EA。

2. 发明授权

US06770451B2 Screening for enzyme inhibitors 有权
标题翻译：筛选酶抑制剂
公开(公告)号：US06770451B2
公开(公告)日：2004-08-03
申请号：US10137952
申请日：2002-05-03
申请人： Riaz Rouhani , Inna Vainshtein
发明人： Riaz Rouhani , Inna Vainshtein
IPC分类号： C12Q148
CPC分类号： C07D498/22 , C12Q1/00 , C12Q1/34 , C12Q1/485 , G01N33/542 , G01N2500/02
摘要： Systems, including methods and reagents, for identifying enzyme inhibitors. The systems employ a conjugate of a known inhibitor of a target enzyme and an enzyme donor, an enzyme acceptor that binds to the enzyme donor to form an active indicator-enzyme complex, and a detectable substrate for the indicator enzyme. The assay is performed by combining the candidate agent, the conjugate of the known inhibitor and enzyme donor, the enzyme acceptor, and the substrate under binding conditions, where the candidate compound competes with the conjugate for the target enzyme. By measuring the rate of product formation or substrate depletion catalyzed by the indicator enzyme, the inhibitory activity of the candidate compound can be determined. The methodology is particularly applicable for target enzymes that have substrates or products that are difficult to synthesize and/or detect, such as kinases and phosphatases.
摘要翻译：用于鉴定酶抑制剂的系统，包括方法和试剂。该系统使用已知的靶酶抑制剂和酶供体的缀合物，与酶供体结合以形成活性指示剂 - 酶复合物的酶受体和用于指示酶的可检测底物。通过将候选试剂，已知抑制剂和酶供体的缀合物，酶受体和底物在结合条件下组合来进行测定，其中候选化合物与目标酶的缀合物竞争。通过测量指示剂催化的产物形成或底物消耗的速率，可以测定候选化合物的抑制活性。该方法特别适用于具有难以合成和/或检测的底物或产物的靶酶，例如激酶和磷酸酶。

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