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1. 发明授权

US5559025A Expression of mature proteinase 2A, the partial purification thereof and preparation of substrates having an inhibitory effect 失效
标题翻译：成熟蛋白酶2A的表达，其部分纯化和具有抑制作用的底物的制备
公开(公告)号：US5559025A
公开(公告)日：1996-09-24
申请号：US320373
申请日：1994-10-11
申请人： Horst Ahorn , Ingrid Maurer-Fogy , Wolfgang Sommergruber , Andreas Zophel , Dieter Blaas , Ernst Kuchler , Hans-Dieter Liebig , Timothy Skern
发明人： Horst Ahorn , Ingrid Maurer-Fogy , Wolfgang Sommergruber , Andreas Zophel , Dieter Blaas , Ernst Kuchler , Hans-Dieter Liebig , Timothy Skern
IPC分类号： A61K38/55 , A61P31/12 , C07K7/06 , C07K7/08 , C07K14/085 , C07K14/81 , C12N1/21 , C12N9/50 , C12N9/99 , C12N15/09 , C12N15/57 , C12R1/19 , C12N15/70
CPC分类号： C07K14/005 , C12N9/506 , C12N2770/32722
摘要： The invention relates to the expression of mature HRV proteinase 2A, the partial purification thereof and the preparation of substrates having an inhibitory effect.
摘要翻译：本发明涉及成熟的HRV蛋白酶2A的表达，其部分纯化以及具有抑制作用的底物的制备。

2. 发明授权

US5196323A Process for preparing and purifying alpha-interferon 失效
标题翻译：制备和纯化α-干扰素的方法
公开(公告)号：US5196323A
公开(公告)日：1993-03-23
申请号：US713618
申请日：1991-06-12
申请人： Gerhard Bodo , Ingrid Maurer-Fogy , Edgar Falkner , Silvia J. Lindner
发明人： Gerhard Bodo , Ingrid Maurer-Fogy , Edgar Falkner , Silvia J. Lindner
IPC分类号： A61K38/00 , C07K14/56
CPC分类号： C07K14/56 , A61K38/00
摘要： A process for the preparation and purification of recombinant Interferon-.alpha. is disclosed. The invention is directed to a process comprising the following steps: cultivating E. coli containing the interferon gene for a growth period during which not more than 5% methionine-interferon is formed; extracting and concentrating the expressed interferon; subjecting the preliminarily purified material to Tandem Chromatography, wherein the Tandem Chromatography comprises separation on a cellulose column followed by an anti-alpha-interferon monoclonal antibody affinity column; subjecting the thus purified material to isoelectric precipitation of impurities at about pH 4.0 to about pH 4.8; and purifying the interferon by chromatography on a high performance cation exchange column using a volatile buffer, wherein the purified interferon is non-immunogenic when administered parenterally to a human.
摘要翻译：公开了重组干扰素-α的制备和纯化方法。本发明涉及一种包括以下步骤的方法：培养含有干扰素基因的大肠杆菌生长期，其中不超过5％的甲硫氨酸 - 干扰素形成; 提取和浓缩表达的干扰素; 将预先纯化的材料进行串联层析，其中串联色谱法包括在纤维素柱上分离，随后是抗α-干扰素单克隆抗体亲和柱; 使如此纯化的材料经受大约pH 4.0至约pH 4.8的杂质的等电沉淀; 并使用挥发性缓冲液在高性能阳离子交换柱上通过色谱法纯化干扰素，其中当肠胃外给予人时，纯化的干扰素是非免疫原性的。

3. 发明授权

US07264944B1 TNF receptors, TNF binding proteins and DNAs coding for them 失效
标题翻译： TNF受体，TNF结合蛋白和编码它们的DNA
公开(公告)号：US07264944B1
公开(公告)日：2007-09-04
申请号：US09525998
申请日：2000-03-15
申请人： Rudolf Hauptmann , Adolf Himmler , Ingrid Maurer-Fogy , Christian Stratowa
发明人： Rudolf Hauptmann , Adolf Himmler , Ingrid Maurer-Fogy , Christian Stratowa
IPC分类号： C12N15/00 , C12N15/09
CPC分类号： C07K14/7151 , A61K38/00 , C12Q1/6886 , C12Q2600/158 , G01N33/6863 , Y02A50/473
摘要： The present invention provides DNA sequences coding for a TNF-binding protein and for the TNF receptor of which this protein constitutes the soluble domain. The DNA sequences can be used for preparing recombinant DNA molecules in order to produce TNF-binding protein and TNF receptor. Recombinant TNF-binding protein is used in pharmaceutical preparations for treating indications in which TNF has a harmful effect. With the aid of the TNF receptor or fragments thereof or with the aid of suitable host organisms transformed with recombinant DNA molecules containing the DNA which codes for the TNF receptor or fragments or modifications thereof, it is possible to investigate substances for their interaction with the TNF receptor and/or for their effect on the biological activity of TNF.
摘要翻译：本发明提供了编码TNF结合蛋白的DNA序列和该蛋白构成可溶性结构域的TNF受体。 DNA序列可用于制备重组DNA分子，以产生TNF结合蛋白和TNF受体。重组TNF结合蛋白用于治疗TNF具有有害作用的适应症的药物制剂中。借助于TNF受体或其片段或借助含有编码TNF受体的DNA的重组DNA分子或其片段或修饰物转化的合适的宿主生物，可以研究它们与TNF相互作用的物质受体和/或其对TNF的生物活性的影响。

4. 发明授权

US06294352B1 TNF receptors, TNF binding proteins and DNAs coding for them 失效
标题翻译： TNF受体，TNF结合蛋白和编码它们的DNA
公开(公告)号：US06294352B1
公开(公告)日：2001-09-25
申请号：US08383676
申请日：1995-02-01
申请人： Rudolf Hauptmann , Adolf Himmler , Ingrid Maurer-Fogy , Christian Stratowa
发明人： Rudolf Hauptmann , Adolf Himmler , Ingrid Maurer-Fogy , Christian Stratowa
IPC分类号： C12N1500
CPC分类号： G01N33/57484 , A61K38/00 , C07K14/7151 , C12Q1/6886 , C12Q2600/136 , G01N33/6863 , Y02A50/473 , Y10S930/12 , Y10S930/144
摘要： DNA sequences coding for a TNF-binding protein and for the TNF receptor of which this protein constitutes the soluble domain. The DNA sequence can be used for preparing recombinant DNA molecules in order to produce TNF-binding protein and TNF receptor. Recombinant TNF-binding protein is used in pharmaceutical preparations for treating indications in which TNF has a harmful effect. With the aid of the TNF receptor or fragments thereof or with the aid of suitable host organisms transformed with recombinant DNA molecules containing the DNA which codes for the TNF receptor or fragments or modifications thereof, it is possible to investigate substances for their interaction with the TNF receptor and/or for their effect on the biological activity of TNF.
摘要翻译：编码TNF结合蛋白的DNA序列和该蛋白质构成可溶性结构域的TNF受体。 DNA序列可用于制备重组DNA分子，以产生TNF结合蛋白和TNF受体。重组TNF结合蛋白用于治疗TNF具有有害作用的适应症的药物制剂中。借助于TNF受体或其片段或借助含有编码TNF受体的DNA的重组DNA分子或其片段或修饰物转化的合适的宿主生物，可以研究它们与TNF相互作用的物质受体和/或其对TNF的生物活性的影响。

5. 发明授权

US06221675B1 TNF receptors, TNF binding proteins and DNAs coding for them 失效
标题翻译： TNF受体，TNF结合蛋白和编码它们的DNA
公开(公告)号：US06221675B1
公开(公告)日：2001-04-24
申请号：US08477639
申请日：1995-06-07
申请人： Rudolf Hauptmann , Adolf Himmler , Ingrid Maurer-Fogy , Christian Stratowa
发明人： Rudolf Hauptmann , Adolf Himmler , Ingrid Maurer-Fogy , Christian Stratowa
IPC分类号： G01N33566
CPC分类号： G01N33/6863 , A61K38/00 , C07K14/7151 , C12Q1/6886 , C12Q2600/136 , G01N33/57484
摘要： DNA sequences coding for a TNF-binding protein and for the TNF receptor of which this protein constitutes the soluble domain. The DNA sequences can be used for preparing recombinant DNA molecules in order to produce TNF-binding protein and TNF receptor. With the aid of the TNF receptor or fragments thereof or with the aid of suitable host organisms transformed with recombinant DNA molecules containing the DNA which codes for the TNF receptor or fragments or modifications thereof, it is possible to investigate substances for their interaction with the TNF is receptor and/or for their effect on the biological activity of TNF.
摘要翻译：编码TNF结合蛋白的DNA序列和该蛋白质构成可溶性结构域的TNF受体。 DNA序列可用于制备重组DNA分子，以产生TNF结合蛋白和TNF受体。借助于TNF受体或其片段或借助含有编码TNF受体的DNA的重组DNA分子或其片段或修饰物转化的合适的宿主生物，可以研究它们与TNF相互作用的物质是受体和/或其对TNF的生物活性的影响。

6. 发明授权

US06440693B1 TNF receptors, TNF binding proteins and DNAS coding for them 失效
标题翻译： TNF受体，TNF结合蛋白和编码它们的DNAS
公开(公告)号：US06440693B1
公开(公告)日：2002-08-27
申请号：US08484307
申请日：1995-06-07
申请人： Rudolf Hauptmann , Adolf Himmler , Ingrid Maurer-Fogy , Christian Stratowa
发明人： Rudolf Hauptmann , Adolf Himmler , Ingrid Maurer-Fogy , Christian Stratowa
IPC分类号： C12N1509
CPC分类号： G01N33/57484 , A61K38/00 , C07K14/7151 , C12Q1/6886 , C12Q2600/136 , G01N33/6863 , Y02A50/473 , Y10S930/12 , Y10S930/144
摘要： DNA sequences coding for a TNF-binding protein and for the TNF receptor of which this protein constitutes the soluble domain. The DNA sequences can be used for preparing recombinant DNA molecules in order to produce TNF-binding protein and TNF receptor. Recombinant TNF-binding protein is used in pharmaceutical preparations for treating indications in which TNF has a harmful effect. With the aid of the TNF receptor or fragments thereof or with the aid of suitable host organisms transformed with recombinant DNA molecules containing the DNA which codes for the TNF receptor or fragments or modifications thereof, it is possible to investigate substances for their interaction with the TNF receptor and/or for their effect on the biological activity of TNF.
摘要翻译：编码TNF结合蛋白的DNA序列和该蛋白质构成可溶性结构域的TNF受体。 DNA序列可用于制备重组DNA分子，以产生TNF结合蛋白和TNF受体。重组TNF结合蛋白用于药物制剂中，用于治疗TNF具有有害作用的适应症。借助于TNF受体或其片段或借助含有编码TNF受体的DNA的重组DNA分子或其片段或修饰物转化的合适的宿主生物，可以研究它们与TNF相互作用的物质受体和/或其对TNF的生物活性的影响。

7. 发明授权

US5710027A Process and vector for expressing alpha-interferon in E. coli 失效
标题翻译：用于在大肠杆菌中表达α-干扰素的方法和载体
公开(公告)号：US5710027A
公开(公告)日：1998-01-20
申请号：US249671
申请日：1994-05-26
申请人： Rudolf Hauptmann , Edgar Falkner , Gerhard Bodo , Tilman Voss , Ingrid Maurer-Fogy
发明人： Rudolf Hauptmann , Edgar Falkner , Gerhard Bodo , Tilman Voss , Ingrid Maurer-Fogy
IPC分类号： C12N15/09 , C07K1/18 , C07K1/20 , C07K1/22 , C07K14/245 , C07K14/52 , C07K14/555 , C07K14/56 , C12N15/21 , C12N15/70 , C12P21/02 , C12R1/19
CPC分类号： C12N15/70 , C07K14/245 , C07K14/56 , C07K2319/02
摘要： Methods and vectors for expressing interferon-alpha (IFN-.alpha.) proteins in E. coli are provided. Use of a vector comprising an IFN-.alpha. sequence fused to an E. coli heat-stable enterotoxin signal sequence (STII) under the control of the E. coli phosphatase (phoA) promoter affords high levels of correctly-folded and -processed, biologically active IFN-.alpha. polypeptides.
摘要翻译：提供了用于在大肠杆菌中表达干扰素-α（IFN-α）蛋白的方法和载体。在大肠杆菌磷酸酶（phoA）启动子控制下，包含与大肠杆菌热稳定肠毒素信号序列（STII）融合的IFN-α序列的载体的使用提供了高水平的正确折叠和加工的生物学活性IFN-α多肽。

8. 发明授权

US5240847A Human manganese superoxide dismutase (hMn-SOD) 失效
标题翻译：人造锰超氧化物歧化酶（HMN-SOD）
公开(公告)号：US5240847A
公开(公告)日：1993-08-31
申请号：US167261
申请日：1988-03-11
申请人： Konrad Heckl , Walter Spevak , Elinborg Ostermann , Andreas Zophel , Edeltraud Krystek , Ingrid Maurer-Fogy , Maria J. Wiche-Castanon , Christian Stratowa , Rudolf Hauptmann
发明人： Konrad Heckl , Walter Spevak , Elinborg Ostermann , Andreas Zophel , Edeltraud Krystek , Ingrid Maurer-Fogy , Maria J. Wiche-Castanon , Christian Stratowa , Rudolf Hauptmann
IPC分类号： C12N1/20 , A61K38/44 , A61K38/45 , A61P29/00 , A61P37/08 , C07H21/04 , C07K14/00 , C12N1/16 , C12N1/19 , C12N1/21 , C12N9/02 , C12N15/09 , C12N15/53 , C12N15/62 , C12P21/00 , C12R1/19 , C12R1/865 , C12R1/91
CPC分类号： C12N9/0089 , C12N15/625 , C07K2319/02 , C07K2319/07
摘要： The present invention relates to a method of preparing human Mn-superoxide dismutase (hMn-SOD) by genetic engineering, the DNA sequences which code for this enzyme, suitable vectors which contain these DNA sequences and host cells which can express these DNA sequences, and the enzyme hMn-SOD itself. Suggestions as to the use of this enzyme are also described.
摘要翻译：本发明涉及通过遗传工程制备人类Mn超氧化物歧化酶（hMn-SOD）的方法，编码该酶的DNA序列，含有这些DNA序列的合适载体和可表达这些DNA序列的宿主细胞，以及酶hMn-SOD本身。还描述了关于使用该酶的建议。

9. 发明授权

US07282483B2 Method of ameliorating the harmful effects of TNF using a polypeptide having the ability to bind to TNF 失效
标题翻译：使用具有结合TNF能力的多肽来改善TNF的有害作用的方法
公开(公告)号：US07282483B2
公开(公告)日：2007-10-16
申请号：US09898234
申请日：2001-07-03
申请人： Rudolf Hauptmann , Adolf Himmler , Ingrid Maurer-Fogy , Christian Stratowa
发明人： Rudolf Hauptmann , Adolf Himmler , Ingrid Maurer-Fogy , Christian Stratowa
IPC分类号： A61K38/16 , C07K14/715
CPC分类号： C07K14/7151 , A61K38/00 , C12Q1/6886 , C12Q2600/158 , G01N33/6863 , Y02A50/473
摘要： The present invention provides DNA sequences coding for a TNF-binding protein and for the TNF receptor of which this protein constitutes the soluble domain. The DNA sequences can be used for preparing recombinant DNA molecules in order to produce TNF-binding protein and TNF receptor. Recombinant TNF-binding protein is used in pharmaceutical preparations for treating indications in which TNF has a harmful effect. With the aid of the TNF receptor or fragments thereof or with the aid of suitable host organisms transformed with recombinant DNA molecules containing the DNA which codes for the TNF receptor or fragments or modifications thereof, it is possible to investigate substances for their interaction with the TNF receptor and/or for their effect on the biological activity of TNF.
摘要翻译：本发明提供了编码TNF结合蛋白的DNA序列和该蛋白构成可溶性结构域的TNF受体。 DNA序列可用于制备重组DNA分子，以产生TNF结合蛋白和TNF受体。重组TNF结合蛋白用于治疗TNF具有有害作用的适应症的药物制剂中。借助于TNF受体或其片段或借助含有编码TNF受体的DNA的重组DNA分子或其片段或修饰物转化的合适的宿主生物，可以研究它们与TNF相互作用的物质受体和/或其对TNF的生物活性的影响。

10. 发明授权

US06417158B1 Method of ameliorating the harmful effects of TNF using a polypeptide having the ability to bind to TNF 失效
标题翻译：使用具有结合TNF能力的多肽来改善TNF的有害作用的方法
公开(公告)号：US06417158B1
公开(公告)日：2002-07-09
申请号：US08477638
申请日：1995-06-07
申请人： Rudolf Hauptmann , Adolf Himmler , Ingrid Maurer-Fogy , Christian Stratowa
发明人： Rudolf Hauptmann , Adolf Himmler , Ingrid Maurer-Fogy , Christian Stratowa
IPC分类号： A61K3819
CPC分类号： G01N33/57484 , A61K38/00 , C07K14/7151 , C12Q1/6886 , C12Q2600/136 , G01N33/6863 , Y02A50/473 , Y10S930/12 , Y10S930/144
摘要： DNA sequences coding for a TNF-binding protein and for the TNF receptor of which this protein constitutes the soluble domain. The DNA sequences can be used for preparing recombinant DNA molecules in order to produce TNF-binding protein and TNF receptor. Recombinant TNF-binding protein is used in pharmaceutical preparations for treating indications in which TNF has a harmful effect. With the aid of the TNF receptor or fragments thereof or with the aid of suitable host organisms transformed with recombinant DNA molecules containing the DNA which codes for the TNF receptor or fragments or modifications thereof, it is possible to investigate substances for their interaction with the TNF receptor and/or for their effect on the biological activity of TNF.
摘要翻译：编码TNF结合蛋白的DNA序列和该蛋白质构成可溶性结构域的TNF受体。 DNA序列可用于制备重组DNA分子，以产生TNF结合蛋白和TNF受体。重组TNF结合蛋白用于治疗TNF具有有害作用的适应症的药物制剂中。借助于TNF受体或其片段或借助含有编码TNF受体的DNA的重组DNA分子或其片段或修饰物转化的合适的宿主生物，可以研究它们与TNF相互作用的物质受体和/或其对TNF的生物活性的影响。

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