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1. 发明申请

WO2013160453A3 ADAPTER MOLECULE CAPABLE OF REVERSIBLY EQUIPPING A FUSION PROTEIN CARRYING AN OLIGOHISTIDINE AFFINITY TAG WITH A FURTHER AFFINITY TAG AND METHODS OF USING THE SAME 审中-公开
标题翻译：具有可逆性的适配器分子携带具有进一步亲和性标签的寡聚物亲和性标签的融合蛋白质及其使用方法
公开(公告)号：WO2013160453A3
公开(公告)日：2014-02-20
申请号：PCT/EP2013058745
申请日：2013-04-26
申请人： IBA GMBH
发明人： SCHMIDT THOMAS , KOTH CHRISTOPHER
IPC分类号： C07K1/13 , C07K1/22 , C07K14/36 , G01N33/68
CPC分类号： C07K1/22 , C07K1/13 , C07K14/00 , C07K14/36 , C07K2319/00 , C07K2319/21 , C07K2319/22 , C07K2319/40 , C07K2319/43 , G01N33/58 , G01N33/68
摘要： Disclosed is a bifunctional adapter molecule comprising two binding moieties A and B, the adapter molecule being capable of reversibly equipping a fusion protein carrying an oligohistidine affinity tag with a further affinity tag, wherein the binding moiety A comprises at least two chelating groups K, wherein each chelating group is capable of binding to a transition metal ion, thereby rendering moiety A capable of binding to an oligohistidine affinity tag, and the binding moiety B is an affinity tag other than an oligohistidine tag.
摘要翻译：公开了包含两个结合部分A和B的双功能衔接子分子，衔接子分子能够可逆地装载携带另外亲和标签的寡组氨酸亲和标签的融合蛋白，其中结合部分A包含至少两个螯合基团K，其中每个螯合基团能够结合过渡金属离子，从而使得能够结合寡组氨酸亲和标签的部分A，并且结合部分B是除寡组氨酸标签之外的亲和标签。

2. 发明申请

WO2013011011A3 METHOD OF REVERSIBLY STAINING A TARGET CELL 审中-公开
标题翻译：反向污染目标细胞的方法
公开(公告)号：WO2013011011A3
公开(公告)日：2013-05-02
申请号：PCT/EP2012063969
申请日：2012-07-17
申请人： IBA GMBH , SCHMIDT THOMAS , STEMBERGER CHRISTIAN , BUSCH DIRK H , GERMEROTH LOTHAR
发明人： SCHMIDT THOMAS , STEMBERGER CHRISTIAN , BUSCH DIRK H , GERMEROTH LOTHAR
IPC分类号： C07K14/36 , G01N33/50
CPC分类号： G01N33/56972 , A61K35/14 , A61K35/17 , C07K2319/00 , C07K2319/22 , C07K2319/74 , G01N1/30 , G01N33/557 , G01N33/566 , G01N2333/705 , G01N2458/00
摘要： The present invention relates to methods of reversibly staining a target cell. The invention also relates to methods of isolating a target cell or a target cell population that is defined by the presence of at least one common specific receptor molecule. The invention also provides kits that can be used to carry out the methods of the invention.
摘要翻译：本发明涉及对靶细胞进行可逆染色的方法。本发明还涉及分离由至少一种常见特异性受体分子的存在定义的靶细胞或靶细胞群的方法。本发明还提供可用于实施本发明方法的试剂盒。

3. 发明申请

WO02054065A3 REVERSIBLE MHC MULTIMER STAINING FOR FUNCTIONAL PURIFICATION OF ANTIGEN-SPECIFIC T CELLS 审中-公开
标题翻译：用于功能性纯化抗原特异性T细胞的可逆MHC多聚体染色
公开(公告)号：WO02054065A3
公开(公告)日：2002-11-28
申请号：PCT/EP0115353
申请日：2001-12-28
申请人： IBA GMBH , BUSCH DIRK H , WAGNER HERMANN
发明人： BUSCH DIRK H , WAGNER HERMANN
IPC分类号： G01N33/48 , A61K35/12 , A61K39/00 , C07K7/06 , C07K14/74 , C07K19/00 , C12N5/0783 , G01N33/50 , G01N33/569 , C12N5/06
CPC分类号： G01N33/56972 , A61K2035/124 , A61K2039/5158 , C12N5/0636 , G01N33/505 , G01N33/5094
摘要： The present invention relates to a new method for reversible staining and functional isolation or characterization of cells, e.g. antigen-specific T cells. With this technique, the original functional status of cells can be substantially maintained after their identification and purification. Thus, this new method is of broad benefit for basic research and clinical applications.
摘要翻译：本发明涉及可逆染色和功能分离或表征细胞的新方法，例如，抗原特异性T细胞。通过这种技术，细胞的原始功能状态在其鉴定和纯化后可以被基本保持。因此，这种新方法对于基础研究和临床应用具有广泛的益处。

4. 发明申请

WO2014076277A1 STREPTAVIDIN MUTEINS AND METHODS OF USING THEM 审中-公开
标题翻译： STREPTAVIDIN MUTEINS和使用它们的方法
公开(公告)号：WO2014076277A1
公开(公告)日：2014-05-22
申请号：PCT/EP2013/074070
申请日：2013-11-18
申请人： IBA GMBH
发明人： SCHMIDT, Thomas
IPC分类号： C07K14/36
CPC分类号： C07K14/36 , C07K1/22 , C07K2319/22 , C07K2319/70 , C12N15/62 , G01N33/68 , G01N2333/36
摘要： The invention concerns novel streptavidin muteins. In one embodiment such a mutein (a) contains at least one mutation in the region of the amino acid positions 115 to 121 with reference to the amino acid sequence of wild type streptavidin as set forth at SEQ ID NO: 15 and (b) has a higher binding affinity than each of (i) a streptavidin mutein that comprises the amino acid sequence Val44 -Thr45- Ala46-Arg47 (SEQ ID NO: 98), or (ii) a streptavidin mutein that comprises the amino acid sequence He44-Gly45- Ala46-Arg47 (SEQ ID NO: 99) at amino acid positions 44 to 47, or (iii) wild type-streptavidin (SEQ ID NO: 15) for peptide ligands comprising the amino acid sequence Trp-Ser-His-Pro-Gln- Phe-Glu-Lys (SEQ ID NO: 100).
摘要翻译：本发明涉及新型链霉亲和素突变蛋白。在一个实施方案中，这样的突变蛋白（a）在氨基酸位置115至121的区域中包含参考SEQ ID NO：15所示的野生型链霉亲和素的氨基酸序列的至少一个突变，（b）具有（i）包含氨基酸序列Val44-Thr45-Ala46-Arg47（SEQ ID NO：98）的链霉抗生物素蛋白突变蛋白（i）链霉亲和素突变蛋白的结合亲和力高，或（ii）包含氨基酸序列He44-Gly45 在氨基酸序列44至47的Ala46-Arg47（SEQ ID NO：99），或（iii）包含氨基酸序列Trp-Ser-His-Pro-的肽配体的野生型链霉亲和素（SEQ ID NO：15） Gln-Phe-Glu-Lys（SEQ ID NO：100）。

5. 发明申请

WO2002077018A1 SEQUENTIALLY ARRANGED STREPTAVIDIN-BINDING MODULES AS AFFINITY TAGS 审中-公开
标题翻译：顺应性地将结合模块作为AFFINITY标签
公开(公告)号：WO2002077018A1
公开(公告)日：2002-10-03
申请号：PCT/EP2001/011846
申请日：2001-10-12
申请人： IBA GMBH , SCHMIDT, Thomas
发明人： SCHMIDT, Thomas
IPC分类号： C07K14/00
CPC分类号： C07K14/36 , C07K2319/00
摘要： The present invention relates to sequentially arranged streptavidin-binding binding modules which may in particular be used as affinity tags. The affinity tags comprise at least two individual modules capable of mediating avidic binding to streptavidin.
摘要翻译：本发明涉及可以特别用作亲和标签的顺序排列的链霉亲和素结合结合模块。亲和标签包含至少两个能够介导与链霉亲和素的鸟氨酸结合的单独模块。

6. 发明申请

WO2015166049A1 METHOD OF ISOLATING A TARGET CELL 审中-公开
标题翻译：分离目标细胞的方法
公开(公告)号：WO2015166049A1
公开(公告)日：2015-11-05
申请号：PCT/EP2015/059510
申请日：2015-04-30
申请人： IBA GMBH
发明人： CARL, Uwe D.
IPC分类号： G01N33/543 , G01N33/566
CPC分类号： G01N33/54306 , C07K16/28 , G01N33/543 , G01N33/566 , G01N2333/705
摘要： The invention provides new methods of isolating target cells using a solid phase, the solid phase comprising a ligand L wherein the ligand L is capable of specifically binding a ligand binding partner LB, the ligand binding partner LB being present in a receptor molecule binding reagent or a multimerization reagent used for isolating target cells. The invention also provides corresponding new arrangements and devices for isolating a target cell from a sample.
摘要翻译：本发明提供了使用固相分离靶细胞的新方法，固相包含配体L，其中配体L能够特异性结合配体结合配偶体LB，配体结合配偶体LB存在于受体分子结合试剂中，或用于分离靶细胞的多聚化试剂。本发明还提供了用于从样品中分离靶细胞的相应新的布置和装置。

7. 发明申请

WO2013160453A2 ADAPTER MOLECULE CAPABLE OF REVERSIBLY EQUIPPING A FUSION PROTEIN CARRYING AN OLIGOHISTIDINE AFFINITY TAG WITH A FURTHER AFFINITY TAG AND METHODS OF USING THE SAME 审中-公开
标题翻译：具有可逆性的适配器分子能够携带具有进一步亲和性标签的寡聚物亲和性标签的融合蛋白质及其使用方法
公开(公告)号：WO2013160453A2
公开(公告)日：2013-10-31
申请号：PCT/EP2013/058745
申请日：2013-04-26
申请人： IBA GMBH
发明人： SCHMIDT, Thomas , KOTH, Christopher
IPC分类号： C07K1/13
CPC分类号： C07K1/22 , C07K1/13 , C07K14/00 , C07K14/36 , C07K2319/00 , C07K2319/21 , C07K2319/22 , C07K2319/40 , C07K2319/43 , G01N33/58 , G01N33/68
摘要： Disclosed is a bifunctional adapter molecule comprising two binding moieties A and B, the adapter molecule being capable of reversibly equipping a fusion protein carrying an oligohistidine affinity tag with a further affinity tag, wherein the binding moiety A comprises at least two chelating groups K, wherein each chelating group is capable of binding to a transition metal ion, thereby rendering moiety A capable of binding to an oligohistidine affinity tag, and the binding moiety B is an affinity tag other than an oligohistidine tag.
摘要翻译：公开了包含两个结合部分A和B的双功能衔接子分子，衔接子分子能够可逆地装载携带另外亲和标签的寡组氨酸亲和标签的融合蛋白，其中结合部分A包含至少两个螯合基团K，其中每个螯合基团能够结合过渡金属离子，从而使得能够结合寡聚组氨酸亲和标签的部分A，并且结合部分B是除寡组氨酸标签之外的亲和标签。

8. 发明申请

WO2006072593A3 DEVICE FOR MAGNET ASSISTED TRANSFER OF CHEMICAL COMPOUNDS INTO CELLS AND METHOD FOR MAGNET ASSISTED TRANSFER OF PROTEINS INTO CELLS 审中-公开
标题翻译：用于将化学化合物磁转移到细胞中的装置和用于将蛋白质磁性转移到细胞中的方法
公开(公告)号：WO2006072593A3
公开(公告)日：2006-12-21
申请号：PCT/EP2006000107
申请日：2006-01-09
申请人： IBA GMBH , SCHMIDT THOMAS , GERMEROTH LOTHAR
发明人： SCHMIDT THOMAS , GERMEROTH LOTHAR
IPC分类号： C12N15/87 , H01F7/02
CPC分类号： C12N15/87 , A61K41/00 , A61K47/6923 , A61K47/6929 , C12M35/06
摘要： Disclosed is method for transferring a protein into a cell comprising contacting a protein to be transferred with at least one magnetic particle to form a complex comprising the protein and the magnetic particle, and contacting said complex with a cell in the presence of a suitable permanent magnetic field, thereby transferring the protein into the cell. Also disclosed is such a complex as well as to methods for making it. Further disclosed is a device for magnet assisted transfer or proteins, nucleic acids and other substances, wherein the device comprises a plurality of permanent magnets arranged adjacent to each other in a substantially gap-free and continuous arrangement that creates a substantially homogeneous magnetic surface. Furthermore, disclosed are methods, compositions and kits useful for research, diagnostics and/or therapy.
摘要翻译：公开了用于将蛋白质转移到细胞中的方法，该方法包括使要转移的蛋白质与至少一个磁性颗粒接触以形成包含蛋白质和磁性颗粒的复合物，并且使所述复合物与细胞在合适的永磁场，从而将蛋白质转移到细胞中。还公开了这种复合物以及制备它的方法。还公开了用于磁体辅助转移或蛋白质，核酸和其他物质的装置，其中所述装置包括多个永磁体，所述多个永磁体以基本上无间隙且连续的布置彼此相邻布置，从而形成基本均匀的磁性表面。此外，公开了用于研究，诊断和/或治疗的方法，组合物和试剂盒。

9. 发明申请

WO2017186669A1 STREPTAVIDIN MUTEINS AND METHODS OF USING THEM 审中-公开
标题翻译： STREPTAVIDIN MUTEINS及其使用方法
公开(公告)号：WO2017186669A1
公开(公告)日：2017-11-02
申请号：PCT/EP2017/059715
申请日：2017-04-25
申请人： IBA GMBH
发明人： SCHMIDT, Thomas
IPC分类号： C07K14/36 , C07K17/00
摘要： The invention concerns novel streptavidin muteins and their use for determination, isolation or purification of proteins under denaturing conditons.In one embodiment such a mutein has an Cys residue at sequence position 127 of the wild-type sequence of streptavidin and comprises at least one mutation in the region of the amino acid positions 115 to 121 with reference to the amino acid sequence of wild type streptavidin.
摘要翻译：本发明涉及新的链霉抗生物素蛋白突变蛋白及其在变性条件下用于测定，分离或纯化蛋白质的用途。在一个实施方案中，这种突变蛋白在野生型序列的第127位具有Cys残基链霉抗生物素蛋白的氨基酸序列，并且在参考野生型链霉抗生物素蛋白的氨基酸序列的氨基酸位置115至121的区域中包含至少一个突变。

10. 发明申请

WO2006072593A2 DEVICE FOR MAGNET ASSISTED TRANSFER OF CHEMICAL COMPOUNDS INTO CELLS AND METHOD FOR MAGNET ASSISTED TRANSFER OF PROTEINS INTO CELLS 审中-公开
标题翻译：用于磁铁辅助化学化合物转移到细胞中的装置和用于磁体辅助将蛋白质转移到细胞中的方法
公开(公告)号：WO2006072593A2
公开(公告)日：2006-07-13
申请号：PCT/EP2006/000107
申请日：2006-01-09
申请人： IBA GMBH , SCHMIDT, Thomas , GERMEROTH, Lothar
发明人： SCHMIDT, Thomas , GERMEROTH, Lothar
IPC分类号： C12N15/87
CPC分类号： C12N15/87 , A61K41/00 , A61K47/6923 , A61K47/6929 , C12M35/06
摘要： Disclosed is method for transferring a protein into a cell comprising contacting a protein to be transferred with at least one magnetic particle to form a complex comprising the protein and the magnetic particle, and contacting said complex with a cell in the presence of a suitable permanent magnetic field, thereby transferring the protein into the cell. Also disclosed is such a complex as well as to methods for making it. Further disclosed is a device for magnet assisted transfer or proteins, nucleic acids and other substances, wherein the device comprises a plurality of permanent magnets arranged adjacent to each other in a substantially gap-free and continuous arrangement that creates a substantially homogeneous magnetic surface. Furthermore, disclosed are methods, compositions and kits useful for research, diagnostics and/or therapy.
摘要翻译：公开了将蛋白质转移到细胞中的方法，包括将待转移的蛋白质与至少一种磁性颗粒接触以形成包含蛋白质和磁性颗粒的复合物，并在合适的永磁体存在下使所述配合物与细胞接触从而将蛋白质转移到细胞中。还公开了这样一个复杂的以及用于制造它的方法。还公开了一种用于磁辅助转移或蛋白质，核酸和其他物质的装置，其中该装置包括以基本上无间隙和连续的布置相邻布置的多个永磁体，其形成基本上均匀的磁性表面。此外，公开了可用于研究，诊断和/或治疗的方法，组合物和试剂盒。

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