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1. 发明授权

US08088899B2 Human granulocyte-colony stimulating factor isoforms 有权
标题翻译：人粒细胞集落刺激因子同种型
公开(公告)号：US08088899B2
公开(公告)日：2012-01-03
申请号：US11917335
申请日：2006-06-13
申请人： Hyung Kon Koh
发明人： Hyung Kon Koh
IPC分类号： A61K38/24 , C07K1/00 , C07K14/00
CPC分类号： A61K38/193 , A61K47/60
摘要： Disclosed is a novel physiologically active protein, which is a human granulocyte colony stimulating factor isoform, constructed in order to increase the in vivo lifetime of human granulocyte colony sedtimulating factor. The human granulocyte colony stimulating factor isoform comprises a polypeptide and polyethylene glycol (PEG) bound thereto as a non-protein polymer. A specific site of the polypeptide is selected so that polyethylene can be bound to the site while not adversely affecting the activity of the protein. The amino acid of the site is modified with cysteine and polyethylene glycol is bound to the modified site. A pharmaceutical composition comprising the isoforms, genes encoding the isoforms, and a primer for modifying the amino acid sequence are also disclosed.
摘要翻译：公开了一种新的生理活性蛋白，其是构建人粒细胞集落刺激因子同种型，以增加人粒细胞集落刺激因子的体内寿命。人粒细胞集落刺激因子同种型包含与非蛋白质聚合物结合的多肽和聚乙二醇（PEG）。选择多肽的特定位点，使得聚乙烯可以结合到该位点而不会不利地影响蛋白质的活性。该位点的氨基酸用半胱氨酸修饰，聚乙二醇与修饰位点结合。还公开了包含同种型，编码同种型的基因和用于修饰氨基酸序列的引物的药物组合物。

2. 发明授权

US07344858B2 Glycosylated human granulocyte colony-stimulating factor (G-CSF) isoform 失效
标题翻译：糖基化的人粒细胞集落刺激因子（G-CSF）同种型
公开(公告)号：US07344858B2
公开(公告)日：2008-03-18
申请号：US10651395
申请日：2003-08-29
申请人： Eun Jung Lee , Hyun Seok Kim , Jong Sang Chung , Ki Wan Kim , Yeon Hyang Kim , Hyune Soo Lee , Hyung Kon Koh , Myung Suk Oh
发明人： Eun Jung Lee , Hyun Seok Kim , Jong Sang Chung , Ki Wan Kim , Yeon Hyang Kim , Hyune Soo Lee , Hyung Kon Koh , Myung Suk Oh
IPC分类号： C12N15/27 , C12N15/66 , C07K14/535 , A61K38/18
CPC分类号： C07K14/535 , A61K38/00
摘要： Disclosed are human G-CSF isoforms having a modified amino acid sequence containing a glycosylation sequence Asn-X-Ser/Thr (N-X-S/T) at one or more specific amino acid positions according to the present invention, genes encoding the human G-CSF isoforms, and expression vectors carrying the genes, eukaryotic cells transformed or transfected with the expression vectors. Also, the present invention discloses a method of preparing a glycosylated human G-CSF isoform, comprising the steps of culturing the transformant or transfectant and isolating a glycosylated human G-CSF isoform from the culture supernatant or cell lysates, a human G-CSF isoform prepared by the method, and a pharmaceutical composition comprising the human G-CSF isoform.
摘要翻译：公开了具有在本发明的一个或多个特定氨基酸位置上含有糖基化序列Asn-X-Ser / Thr（NXS / T）的修饰氨基酸序列的人G-CSF同种型，编码人G-CSF的基因同种型和携带基因的表达载体，用表达载体转化或转染的真核细胞。此外，本发明公开了一种制备糖基化的人G-CSF同工型的方法，其包括培养转化体或转染子并从培养上清或细胞裂解物，人G-CSF同工型分离糖基化的人G-CSF同种型的步骤通过该方法制备，以及包含人G-CSF同种型的药物组合物。

3. 发明申请

US20100143288A1 HUMAN GRANULOCYTE-COLONY STIMULATING FACTOR ISOFORMS 有权
标题翻译：人造粒组织刺激因子
公开(公告)号：US20100143288A1
公开(公告)日：2010-06-10
申请号：US11917335
申请日：2006-06-13
申请人： Hyung Kon Koh
发明人： Hyung Kon Koh
IPC分类号： A61K38/19 , C07K14/535 , C07H21/04
CPC分类号： A61K38/193 , A61K47/60
摘要： Disclosed is a novel physiologically active protein, which is a human granulocyte colony stimulating factor isoform, constructed in order to increase the in vivo lifetime of human granulocyte colony sedtimulating factor. The human granulocyte colony stimulating factor isoform comprises a polypeptide and polyethylene glycol (PEG) bound thereto as a non-protein polymer. A specific site of the polypeptide is selected so that polyethylene can be bound to the site while not adversely affecting the activity of the protein. The amino acid of the site is modified with cysteine and polyethylene glycol is bound to the modified site. A pharmaceutical composition comprising the isoforms, genes encoding the isoforms, and a primer for modifying the amino acid sequence are also disclosed.
摘要翻译：公开了一种新的生理活性蛋白，其是构建人粒细胞集落刺激因子同种型，以增加人粒细胞集落刺激因子的体内寿命。人粒细胞集落刺激因子同种型包含与非蛋白质聚合物结合的多肽和聚乙二醇（PEG）。选择多肽的特定位点，使得聚乙烯可以结合到该位点而不会不利地影响蛋白质的活性。该位点的氨基酸用半胱氨酸修饰，聚乙二醇与修饰位点结合。还公开了包含同种型，编码同种型的基因和用于修饰氨基酸序列的引物的药物组合物。

4. 发明申请

US20050019871A1 Glycosylated human interferon alpha isoform 失效
标题翻译：糖基化人类干扰素α同种型
公开(公告)号：US20050019871A1
公开(公告)日：2005-01-27
申请号：US10653350
申请日：2003-09-02
申请人： Eun Jung Lee , Hyung Park , Hyun Seok Kim , Ji Sook Park , Yeon Hyang Kim , Hyune Soo Lee , Hyung Kon Koh , Myung Suk Oh
发明人： Eun Jung Lee , Hyung Park , Hyun Seok Kim , Ji Sook Park , Yeon Hyang Kim , Hyune Soo Lee , Hyung Kon Koh , Myung Suk Oh
IPC分类号： C07K14/56 , C12P21/02 , C07H21/04
CPC分类号： C07K14/56
摘要： The present invention relates to an amino acid-modified human interferon alpha isoform having at least one of the Asn-Xaa-Ser/Thr (N-X-S/T) sequence formed at a specific site so that glycosylation takes place at this site and a gene encoding the same, an expression vector comprising the gene, and a method for producing glycosylated human interferon alpha isoform by transforming or transfecting an eukaryotic cell with the expression vector, culturing the transfected or transformed cell and isolating the glycosylated human interferon alpha isoform from the culture, the glycosylated human interferon alpha isoform produced therefrom and a pharmaceutical composition comprising the same.
摘要翻译：本发明涉及具有在特定部位形成的至少一种Asn-Xaa-Ser / Thr（NXS / T）序列的氨基酸修饰的人类干扰素α同种型，使得在该位点发生糖基化，并编码基因相同的包含该基因的表达载体和通过用表达载体转化或转染真核细胞来产生糖基化的人干扰素α同种型的方法，培养转染或转化的细胞并从培养物中分离糖基化的人干扰素α同种型，由其制备的糖基化的人干扰素α同种型和包含其的药物组合物。

5. 发明授权

US07311903B2 Glycosylated human interferon alpha isoform 失效
标题翻译：糖基化人类干扰素α同种型
公开(公告)号：US07311903B2
公开(公告)日：2007-12-25
申请号：US10653350
申请日：2003-09-02
申请人： Eun Jung Lee , Hyung Ki Park , Hyun Seok Kim , Ji Sook Park , Yeon Hyang Kim , Hyune Soo Lee , Hyung Kon Koh , Myung Suk Oh
发明人： Eun Jung Lee , Hyung Ki Park , Hyun Seok Kim , Ji Sook Park , Yeon Hyang Kim , Hyune Soo Lee , Hyung Kon Koh , Myung Suk Oh
IPC分类号： A61K38/21 , C07K17/00
CPC分类号： C07K14/56
摘要： The present invention relates to an amino acid-modified human interferon alpha isoform having at least one of the Asn-Xaa-Ser/Thr (N-X-S/T) sequence formed at a specific site so that glycosylation takes place at this site and a gene encoding the same, an expression vector comprising the gene, and a method for producing glycosylated human interferon alpha isoform by transforming or transfecting an eukaryotic cell with the expression vector, culturing the transfected or transformed cell and isolating the glycosylated human interferon alpha isoform from the culture, the glycosylated human interferon alpha isoform produced therefrom and a pharmaceutical composition comprising the same.
摘要翻译：本发明涉及具有在特定部位形成的至少一种Asn-Xaa-Ser / Thr（NXS / T）序列的氨基酸修饰的人类干扰素α同种型，使得在该位点发生糖基化，并编码基因相同的包含该基因的表达载体和通过用表达载体转化或转染真核细胞来产生糖基化的人干扰素α同种型的方法，培养转染或转化的细胞并从培养物中分离糖基化的人干扰素α同种型，由其制备的糖基化的人干扰素α同种型和包含其的药物组合物。

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