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1. 发明申请

US20100054996A1 MICROCHIP FOR PROTEIN FIXATION 审中-公开
标题翻译： MICROCHIP蛋白质固定
公开(公告)号：US20100054996A1
公开(公告)日：2010-03-04
申请号：US12373877
申请日：2007-07-19
申请人： Hyun Jik Oh , Tae Joong Yoon , Sang Hoon Lee
发明人： Hyun Jik Oh , Tae Joong Yoon , Sang Hoon Lee
IPC分类号： G01N33/53
CPC分类号： G01N33/54313 , G01N33/6848 , G01N2333/4742 , G01N2333/765 , G01N2333/79
摘要： Disclosed is a microchip for protein fixation, which fixes a specific protein, such as albumin, immunoglobulin, transferrin, or keratin, to beads so as to detect a micro amount of proteins during a micro analysis process, such as Liquid Chromatography/Mass Spectrometry (LC/MS) or Matrix Assisted Laser Desorption/Ionization Time Of Flight Mass Spectrometry (MALDI-TOF/MS). The microchip for protein fixation reduces the amount of a sample, shortens a reaction time, heightens the reliability in results, and simplifies a working process. For this reason, the beads provided with an antibody attached thereto are contained in a chamber of the microchip having a stratified structure using an organic polymer, i.e., dimethylsiloxane (PDMS), the pressure of a micro amount of a sample passing through the chamber is uniformly distributed onto all the beads, and the micro amount of the sample passes through the beads having a large surface area and is smoothly discharged to the outside at the optimum speed so as to effectively fix the specific protein. Thus, the microchip for protein fixation optimizes the speed of the sample passing through the microchip, and increases an efficiency of fixing the specific protein to the antibody due to the sufficient surface area of the fine-sized beads, thereby obtaining a rapid reaction result.
摘要翻译：公开了用于蛋白质固定的微芯片，其将特定蛋白质例如白蛋白，免疫球蛋白，转铁蛋白或角蛋白固定在珠上，以便在微量分析过程中检测微量的蛋白质，例如液相色谱/质谱（ LC / MS）或基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF / MS）。用于蛋白质固定的微芯片可减少样品量，缩短反应时间，提高结果的可靠性，简化工作过程。因此，使用有机聚合物即二甲基硅氧烷（PDMS）将具有附着抗体的小珠包含在具有分层结构的微芯片的腔室中，通过该腔室的微量样品的压力为均匀分布在所有珠粒上，微量的样品通过具有大表面积的珠粒，并以最佳速度平滑地排出到外部，以有效地固定特定的蛋白质。因此，用于蛋白质固定的微芯片优化了通过微芯片的样品的速度，并且由于细小珠粒的足够的表面积而提高了将特定蛋白质固定到抗体的效率，从而获得快速的反应结果。

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