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1. 发明申请

US20080247627A1 Apparatus and Method For Detecting Protein Crystals 审中-公开
标题翻译：用于检测蛋白质晶体的装置和方法
公开(公告)号：US20080247627A1
公开(公告)日：2008-10-09
申请号：US10593693
申请日：2006-02-27
申请人： Hirofumi Matsuzaki
发明人： Hirofumi Matsuzaki
IPC分类号： G06K9/00
CPC分类号： G06K9/0014
摘要： A protein crystal detecting method for detecting a protein crystal in protein solution held in a crystallizing container, wherein layer images are obtained by imaging cross sections of a protein solution, a crystal feature image for each layer is created by extracting a feature portion of a protein crystal from the layer images, judgment parameters such as a representative number of pixels representing the number of pixels of the layer image showing the largest feature portions, a representative average number of pixels for each label, and a layer-to-layer rate.
摘要翻译：一种用于检测保存在结晶容器中的蛋白质溶液中的蛋白质晶体的蛋白质晶体检测方法，其中通过对蛋白质溶液的横截面进行成像而获得层图像，通过提取蛋白质的特征部分来产生每层的晶体特征图像来自层图像的晶体，诸如表示表示最大特征部分的层图像的像素数的代表数量的像素数，每个标签的代表性平均像素数，以及层到层速率的判断参数。

2. 发明申请

US20060276630A1 Method for detecting protein crystal, apparatus for detecting protein crystal and program for detecting protein crystal 审中-公开
标题翻译：蛋白质晶体检测方法，蛋白质晶体检测装置及蛋白质晶体检测程序
公开(公告)号：US20060276630A1
公开(公告)日：2006-12-07
申请号：US10554579
申请日：2004-04-28
申请人： Hirofumi Matsuzaki , Hideyuki Miyatake , Seong Hoon Kim , Kunio Miki
发明人： Hirofumi Matsuzaki , Hideyuki Miyatake , Seong Hoon Kim , Kunio Miki
IPC分类号： G06F19/00 , C07K14/47
CPC分类号： C30B29/58 , C30B7/00
摘要： It is an object of the present invention to provide a protein crystal detection method, protein crystal detection apparatus, and protein crystal detection program with which protein crystals can be efficiently detected at a high reliability. To detect protein crystals with the present invention, an observed image obtained by observing a protein solution during crystallization by the vapor diffusion method is differentiated to give a differentiated image constituted by brightness change information indicating how much brightness has changed. Noise then is eliminated from this differentiated image, after which the differentiated image is binarized at a threshold for extracting a characterizing portion, which gives a binarized image in which the portion exhibiting a large change in brightness is left behind as the characterizing portion. Crystallization is evaluated by determining whether or not there are protein crystals from this characterizing portion of the binarized image. Thus, protein crystal detection, which up to now has relied exclusively on visual observation by observers, can be carried out efficiently with high reliability.
摘要翻译：本发明的目的是提供蛋白质晶体检测方法，蛋白质晶体检测装置和蛋白质晶体检测程序，可高效地检测蛋白质晶体。为了利用本发明检测蛋白质晶体，通过蒸气扩散法观察结晶期间的蛋白质溶液而获得的观察图像被区分，从而给出由亮度变化信息构成的微分图像，该亮度变化信息表示亮度有多大变化。然后从该微分图像中消除噪声，之后将阈值处的差分图像二值化以提取表征部分，其给出其中表现出大的亮度变化的部分作为特征部分的二值化图像。通过确定来自二值化图像的该特征部分是否存在蛋白质晶体来评价结晶。因此，高效可靠地进行迄今为止完全依靠观察者目视观察的蛋白质晶体检测。

3. 发明申请

US20070020748A1 Apparatus for screening proptein crystallization conditions 审中-公开
标题翻译：筛选proptein结晶条件的装置
公开(公告)号：US20070020748A1
公开(公告)日：2007-01-25
申请号：US10555033
申请日：2004-04-28
申请人： Itsuro Motegi , Hirofumi Matsuzaki , Hideyoshi Kitahara , Mitsuhaya Tsukamoto , Kouji Shimogawa , Masataka Magoori , Akira Higuchi
发明人： Itsuro Motegi , Hirofumi Matsuzaki , Hideyoshi Kitahara , Mitsuhaya Tsukamoto , Kouji Shimogawa , Masataka Magoori , Akira Higuchi
IPC分类号： C12M1/34
CPC分类号： G01N33/68 , B01D9/00 , B01D9/0077 , C30B7/00 , C30B7/02 , C30B29/58 , G01N35/028 , G01N2035/0425
摘要： The present invention is intended to provide an apparatus for screening protein crystallization conditions that can screen protein crystallization conditions efficiently by the vapor diffusion method using the sitting drop technique. In order to achieve the above-mentioned object, in an apparatus for screening protein crystallization conditions that screens protein crystallization conditions using the sitting drop technique that is one of the techniques of protein crystallization to be carried out by the vapor diffusion method, an apparatus 2 for preparing a crystallization plate that includes a dispensing means for dispensing a protein solution and a crystallization solution in wells of the crystallization plate and a seal attachment unit that seals the wells that have been subjected to dispensation is connected to a protein crystal detection apparatus 5 that detects protein crystals produced in the crystallization plate in a thermostatic chamber that stores the crystallization plate that has been subjected to the dispensing in a predetermined environment. Accordingly, the crystallization plate is transferred automatically and thus screening is carried out efficiently and automatically.
摘要翻译：本发明旨在提供一种用于筛选蛋白质结晶条件的装置，其可以通过使用坐式技术的蒸气扩散法有效地筛选蛋白质结晶条件。为了实现上述目的，在使用通过蒸气扩散法进行蛋白质结晶的技术之一的坐式滴定技术筛选蛋白质结晶条件的蛋白质结晶条件的装置中，装置2 用于制备结晶板，其包括用于在结晶板的孔中分配蛋白质溶液和结晶溶液的分配装置和将经过分配的孔密封的密封附着单元连接到蛋白质晶体检测装置5，蛋白质晶体检测装置5 检测在恒温室中在结晶板中产生的蛋白质晶体，所述恒温室存储在预定环境中进行了分散的结晶板。因此，结晶板被自动转印，从而有效且自动地进行筛选。

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