专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08124424B2 Single molecule-format bioluminescent probe 失效
标题翻译：单分子格式生物发光探针
公开(公告)号：US08124424B2
公开(公告)日：2012-02-28
申请号：US12448881
申请日：2008-01-15
申请人： Yoshio Umezawa , Moritoshi Sato , Hiroaki Tao , SungBae Kim
发明人： Yoshio Umezawa , Moritoshi Sato , Hiroaki Tao , SungBae Kim
IPC分类号： G01N33/566 , G01N33/53 , G01N33/567 , C12Q1/66 , C07K1/00 , C12N15/00 , C12N5/00
CPC分类号： G01N33/542
摘要： The claimed invention comprises a single molecule-format bioluminescent probe for detecting a target-specific ligand in a living cell, which comprises, a ligand-binding molecule of which conformation is changed upon binding to the ligand, wherein the ligand-binding molecule comprises a ligand-binding domain (LBD) of a nuclear receptor and an LBD-interacting domain that is a co-activator peptide of said nuclear receptor, and an N-terminal polypeptide and a C-terminal polypeptide of a click beetle luciferase (N-CBLuc and C-CBLuc), which flank each end of the ligand-binding molecule, respectively, wherein the N-CBLuc and the C-CBLuc self-complement to generate a luminescent signal only upon binding of the ligand to the ligand-binding molecule.
摘要翻译：所要求保护的发明包括用于检测活细胞中的靶特异性配体的单分子形式的生物发光探针，其包含在与配体结合时构象改变的配体结合分子，其中配体结合分子包含核受体的配体结合结构域（LBD）和作为所述核受体的共活化剂肽的LBD相互作用结构域，以及点击甲虫荧光素酶的N末端多肽和C-末端多肽（N-CBLuc 和C-CBLuc），其分别位于配体结合分子的每个末端，其中N-CBLuc和C-CBLuc自补体仅在配体结合配体结合分子时产生发光信号。

2. 发明申请

US20090305910A1 Variable pitch array spotter 审中-公开
标题翻译：可变螺距排列器
公开(公告)号：US20090305910A1
公开(公告)日：2009-12-10
申请号：US12309072
申请日：2007-06-27
申请人： Hiroshi Aoki , Masaki Torimura , Hiroaki Tao , Takashi Ikeda
发明人： Hiroshi Aoki , Masaki Torimura , Hiroaki Tao , Takashi Ikeda
IPC分类号： C40B60/00
CPC分类号： G01N35/1067 , B01J2219/00369 , B01J2219/00387 , B01J2219/00659 , B01L3/0262 , B01L9/54 , B01L2200/022 , B01L2300/0819 , B01L2300/0838 , B01L2400/025 , G01N35/1074
摘要： At each lattice point 2 of pantographs 1, a capillary 3 is held perpendicular to the pantograph lattice. The pantograph 1 includes: rings 4 and 5 to serve as lattice points; and shafts 6 linking the rings with one another. Each of the capillaries 3 is supported by the two rings 4 and 5. The shafts are free to rotate about an intersection 7. The ring 5 is fixed to the capillary 3, but the ring 4 is a movable ring being moveable freely up and down in the axial direction of the capillary 3 in conjunction with the pantograph 1. The lattice distance can be determined accomplished by moving up or down the movable ring at an arbitrarily-chosen lattice point in a pantograph 1. Accordingly, all the capillaries move in parallel to one another, and the ends of all the capillaries always stay within a single plane.
摘要翻译：在受电弓1的每个格点2处，毛细管3垂直于受电弓格架保持。受电弓1包括：环4和5作为格点; 以及将环彼此连接的轴6。每个毛细管3由两个环4和5支撑。轴可以绕交叉点7自由旋转。环5固定到毛细管3上，但是环4是可上下移动的可动环在集电弓1上与毛细管3的轴向方向一致。可以通过在受电弓1中任意选择的格点上移动上下移动环来确定晶格距离。因此，所有毛细管平行移动所有毛细血管的末端总是保持在单一的平面内。

3. 发明申请

US20090269781A1 Single-Molecule-Format Probe And Utilization Thereof 有权
标题翻译：单分子格式探测及其利用
公开(公告)号：US20090269781A1
公开(公告)日：2009-10-29
申请号：US12429378
申请日：2009-04-24
申请人： Sung-Bae KIM , Hiroaki TAO , Moritoshi SATO
发明人： Sung-Bae KIM , Hiroaki TAO , Moritoshi SATO
IPC分类号： G01N33/53 , C12N9/96 , C12N15/11 , C12N15/00 , C12N5/06 , C12N15/87
CPC分类号： G01N33/542 , C07K14/721 , C07K2319/61 , C12N9/12 , C12N15/62
摘要： A single-chain probe of the present invention for detecting a ligand, comprises: a ligand binding protein for binding the ligand; a recognition protein for recognizing that the ligand is bound by the ligand binding protein; and C— and N-terminal fragments, generated by dissecting an enzyme, between the ligand binding protein and the recognition protein, wherein a carboxy terminal end of the C-terminal fragment is located upstream of an amino terminal end of the N-terminal fragment, and the C— and N-terminal fragments vary the enzyme activity via complementation in case where the recognition protein recognizes that the ligand is bound by the ligand binding protein. This makes it possible to achieve detection of a target protein-specific ligand using the single chain with a high efficiency.
摘要翻译：用于检测配体的本发明的单链探针包括：用于结合配体的配体结合蛋白; 用于识别配体被配体结合蛋白结合的识别蛋白; 以及通过解剖酶在配体结合蛋白和识别蛋白之间产生的C-和N-末端片段，其中C-末端片段的羧基末端位于N-末端片段的氨基末端的上游在识别蛋白识别配体被配体结合蛋白结合的情况下，C-和N-末端片段通过互补来改变酶活性。这使得可以以高效率实现使用单链的靶蛋白特异性配体的检测。

4. 发明申请

US20090180930A1 DISPENSING APPARATUS AND A DISPENSING METHOD 有权
标题翻译：分配装置和分配方法
公开(公告)号：US20090180930A1
公开(公告)日：2009-07-16
申请号：US12201552
申请日：2008-08-29
申请人： Hiroshi AOKI , Hiroaki TAO , Masaki TORIMURA , Takashi IKEDA
发明人： Hiroshi AOKI , Hiroaki TAO , Masaki TORIMURA , Takashi IKEDA
IPC分类号： G01N33/00 , B01L3/02
CPC分类号： B01L3/0241 , B01L3/0217 , B01L9/52 , B01L2200/022 , B01L2300/0819 , B01L2300/0829 , B01L2300/0838 , B01L2400/0478 , G01N35/1011 , G01N2035/1039
摘要： In a dispensing apparatus for dispensing a liquid, such as a sample solution, on a substrate, such as a glass slide, the dispensing apparatus includes a capillary provided with a distal end and a proximal end, a pump unit configured to pump an operating liquid into the capillary and to pump the operating liquid out of the capillary, and a controller configured to control the pump unit so as to change a position of a liquid surface of the operating liquid in the capillary so that a predetermined volume of liquid is suctioned from the distal end into the capillary and the liquid suctioned in the capillary is discharged from the distal end. As a result, it is possible to precisely dispense extremely small volume amounts of liquids such as a nanoliter.
摘要翻译：在用于将诸如样品溶液的液体分配在诸如玻璃载体的基底上的分配装置中，分配装置包括设置有远端和近端的毛细管，泵单元构造成泵送操作液体进入毛细管并将工作液体泵出毛细管;以及控制器，被配置为控制泵单元，以便改变毛细管中的操作液体的液面的位置，使得预定体积的液体从进入毛细管的远端和毛细管中吸入的液体从远端排出。结果，可以精确地分配非常小量的液体，例如纳升。

5. 发明申请

US20090176239A1 SINGLE-MOLECULE-FORMAT REAL-TIME BIOLUMINESCENCE IMAGING PROBE 失效
标题翻译：单分子格式实时生物显微成像探针
公开(公告)号：US20090176239A1
公开(公告)日：2009-07-09
申请号：US12343830
申请日：2008-12-24
申请人： Sung-Bae KIM , Hiroaki TAO , Moritoshi SATO
发明人： Sung-Bae KIM , Hiroaki TAO , Moritoshi SATO
IPC分类号： C12Q1/68 , C12N9/96 , C12N15/11 , C12N15/00 , C12N5/06 , C12Q1/66
CPC分类号： C12N15/62 , C07K14/4728 , C07K14/721 , C12N9/0069 , C12N9/1205
摘要： The present invention provides an “in vivo and in vitro real-time bioluminescence imaging means,” which can transmit a detection signal promptly in response to an external signal, while taking advantage of a single-molecule-format luminescent probe as a bioluminescent means.The present invention is characterized by using, as a single-molecule-format luminescent probe utilizing the increase and decrease of a second messenger level as an index, a fusion protein including a single-chain protein containing a second messenger recognition protein and optionally a peptide which is capable of binding with the protein, and linked respectively to the N-terminus and the C-terminus thereto, an N-terminal fragment (N-LE) and a C-terminal fragment (C-LE) generated by dissecting a luminescent enzyme (LE). A single-molecule-format luminescent probe could be provided, which makes it possible to observe light emission (extinction) in vivo as well as in vitro, as a result of a conformational change of the second messenger recognition protein induced by the binding (unbinding) with a second messenger, and the subsequent association or dissociation between the N-LE and the C-LE flanked at both termini of the recognition protein.
摘要翻译：本发明提供了一种“体内和体外实时生物发光成像装置”，其可以利用单分子格式的发光探针作为生物发光装置，利用外部信号迅速地发送检测信号。本发明的特征在于，使用以第二信使水平的增加和减少为指标的单分子格式的发光探针，包含含有第二信使识别蛋白和任选的肽的单链蛋白的融合蛋白其能够与蛋白质结合并分别连接到其N末端和C末端，通过解析发光的N末端片段（N-LE）和C-末端片段（C-LE）酶（LE）。可以提供单分子格式的发光探针，这使得可以在体内和体外观察光发射（消光），作为由结合诱导的第二信使识别蛋白（解键合）的构象变化的结果）和第二信使的N-LE和C-LE之间的后续关联或解离，两侧位于识别蛋白的两个末端。

6. 发明申请

US20060255262A1 Ionization plate for mass spectrometry and mass spectrometer 有权
公开(公告)号：US20060255262A1
公开(公告)日：2006-11-16
申请号：US11412701
申请日：2006-04-27
申请人： Hiroaki Sato , Atsushi Yamamoto , Teruyuki Seino , Masaki Torimura , Hiroaki Tao
发明人： Hiroaki Sato , Atsushi Yamamoto , Teruyuki Seino , Masaki Torimura , Hiroaki Tao
IPC分类号： H01J49/00
CPC分类号： B82Y10/00 , H01J49/0418
摘要： Provided are a laser desorption ionization mass spectrometry sample plate for a soft LDI-MS measurement, in which when a laser beam is irradiated, a correct measurement of high sensitivity can be made without generation of any disturbance peak and uniform coating of a sample can be made on a sample plate in fabrication of the sample, and a measurement apparatus using the sample plate. A specified ionization element having a dot structure is used as an ionization medium which is used in laser desorption ionization mass spectrometry and absorbs a laser beam.

7. 发明申请

US20060024199A1 Inductively-coupled plasma torch 有权
标题翻译：感应耦合等离子体焰炬
公开(公告)号：US20060024199A1
公开(公告)日：2006-02-02
申请号：US11184671
申请日：2005-07-19
申请人： Hiroaki Tao , Tetsuya Nakazato , Kenichi Sakata
发明人： Hiroaki Tao , Tetsuya Nakazato , Kenichi Sakata
IPC分类号： G01N27/00
CPC分类号： H01J49/105 , G01N30/7206 , H05H1/30 , H05H1/42
摘要： To provide an inductively-coupled plasma torch capable of realizing a GC analysis or the like and a solution analysis by the same torch without removing a capillary tube, etc. The injector tube of the inductively-coupled plasma torch includes an outer injector tube 11 for introducing an atomized solution sample or the like into the inductively-coupled plasma, and an inner injector tube 12 for introducing gaseous molecules into the inductively-coupled plasma, housed in the outer injector tube as integrated and coaxially with it. The inner injector tube is able to convey make-up gas, and has a capillary tube 4 for conveying gaseous molecules and carrier gas together. For sample introduction in analyzing a gaseous sample, the inner injector tube 12 is used, and for sample introduction in analyzing an aerosol sample, the outer injector tube 11 is used.
摘要翻译：提供能够实现GC分析等的电感耦合等离子体焰炬和通过相同的手电筒进行溶液分析而不去除毛细管等。电感耦合等离子体焰炬的喷射管包括外部喷射管11，其用于将雾化溶液样品等引入电感耦合等离子体，以及内部注射管12，用于将气态分子引入感应耦合等离子体中，该等离子体被容纳在与其一体并与其同轴的外部注射管中。内部注射管能够输送补充气体，并且具有用于将气态分子和载气一起输送的毛细管4。对于分析气体样品的样品介绍，使用内部注射管12，并且为了在分析气溶胶样品时进行样品引入，使用外部注射管11。

8. 发明授权

US06299844B1 Photochemical reactor 有权
标题翻译：光化学反应堆
公开(公告)号：US06299844B1
公开(公告)日：2001-10-09
申请号：US09534620
申请日：2000-03-27
申请人： Hiroaki Tao , Tetsuya Nakazato
发明人： Hiroaki Tao , Tetsuya Nakazato
IPC分类号： B01J1908
CPC分类号： B01J19/123 , B01J2219/00094 , C02F1/325 , C02F2201/3223 , C02F2201/3228
摘要： A photochemical reactor including a lamp for producing light when supplied with electric current; and a transparent pipe secured to and extending through the lamp and having both ends protruding from the lamp so that a reactant feed can be fed from one end of the pipe, irradiated with light from the lamp and discharged from the other end of the pipe.
摘要翻译：一种光化学反应器，包括用于在供应电流时产生光的灯; 以及固定并延伸穿过灯的透明管并且具有从灯突出的两端，使得能够从管的一端供给反应物进料，照射来自灯的光并从管的另一端排出。

9. 发明授权

US5275956A Method of measuring concentration of organic chlorine compound by chemiluminescence 失效
标题翻译：通过化学发光法测定有机氯化合物浓度的方法
公开(公告)号：US5275956A
公开(公告)日：1994-01-04
申请号：US978356
申请日：1992-11-18
申请人： Kenji Bansho , Hiroaki Tao , Akira Miyazaki , Takashi Imagawa
发明人： Kenji Bansho , Hiroaki Tao , Akira Miyazaki , Takashi Imagawa
IPC分类号： G01N21/76 , G01N33/00 , G01N33/18
CPC分类号： G01N33/0049 , G01N21/766 , G01N33/1826 , Y10T436/19 , Y10T436/193333 , Y10T436/196666
摘要： The concentration of an organic chlorine compound contained in a gaseous medium is measured in situ by feeding the gaseous medium to a reaction zone and oxidizing the chlorine compound to produce a reactive gas capable of reacting with a chemiluminescent compound to cause chemiluminescence. The reactive gas is contacted with the chemiluminescent compound contained in a cell to cause chemiluminescence whose intensity is proportional to the amount of the reactive gas produced by the oxidation of the organic compound. The concentration of an organic chlorine compound contained in an aqueous medium may also be measured by the similar method after separating the organic chlorine compound as vapors from the aqueous medium. A device suitable for the above measurement is also disclosed.
摘要翻译：气态介质中含有的有机氯化合物的浓度通过将气体介质供给到反应区而进行原位测量，并氧化氯化合物以产生能与化学发光化合物反应以引起化学发光的反应性气体。反应性气体与细胞中所含的化学发光化合物接触以引起化学发光，其强度与通过有机化合物的氧化产生的反应性气体的量成比例。含水介质中含有的有机氯化合物的浓度也可以通过类似的方法在从水性介质中分离有机氯化合物作为蒸汽之后测量。还公开了适用于上述测量的装置。

10. 发明授权

US08663577B2 Variable pitch array spotter 有权
标题翻译：可变螺距排列器
公开(公告)号：US08663577B2
公开(公告)日：2014-03-04
申请号：US12385755
申请日：2009-04-17
申请人： Hiroshi Aoki , Masaki Torimura , Hiroaki Tao , Takashi Ikeda
发明人： Hiroshi Aoki , Masaki Torimura , Hiroaki Tao , Takashi Ikeda
IPC分类号： B01L3/00
CPC分类号： B01J19/0046 , B01J2219/00369 , B01J2219/00387 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00659 , B01L3/0244 , B01L3/0262 , B01L3/0268 , B01L9/54 , B01L9/547 , B01L2200/022 , B01L2300/0838 , G01N35/1067 , G01N35/1074 , Y10T436/2575
摘要： A spotter that includes a plurality of spotting heads, each of the plurality of spotting heads having a discharging portion at a tip portion, the plurality of spotting heads form an m×n array (m, n>1) with m spotting heads arranged lengthwise and n spotting heads arranged crosswise; and a pitch varying mechanism configured to vary an array pitch of the plurality of spotting heads arrayed in a lengthwise direction and an array pitch of the plurality of spotting heads arrayed in a crosswise direction.
摘要翻译：一种检测器，包括多个点样头，所述多个点样头中的每一个具有在尖端部分的排出部分，所述多个点样头形成m×n阵列（m，n> 1），其中m个点样头纵向排列并且n个点头交叉排列; 以及变桨机构，被配置为改变沿横向方向排列的多个点样头的阵列间距和沿横向排列的多个点样头的阵列间距。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式