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    • 2. 发明授权
    • Composition for separating spermatozoa from a semen sample
    • 用于从精液样品中分离精子的组合物
    • US08939887B2
    • 2015-01-27
    • US12677034
    • 2008-09-05
    • Jane MorrellHeriberto Rodriguez-Martinez
    • Jane MorrellHeriberto Rodriguez-Martinez
    • A61B17/43C12N1/02A61K35/52C12N5/076C12N5/071
    • C12N1/02A61K35/52C12N5/061C12N5/0612
    • Compositions and methods useful for processing animal semen for use in assisted reproduction. A composition, or colloid formulation, for separation of spermatozoa from a semen sample, the composition including at least one salt of an alkali metal and/or an alkaline earth metal, EDTA, a zwitterion buffer, silane-coated silica particles and water, the composition having a pH of 7.0-7.35 and an osmolarity of 300-345 mOsm. A method for preparing spermatozoa from a semen sample from a non-human animal by separating the spermatozoa from other semen constituents by centrifugation through a single layer of the composition, or colloid formulation. Also, a method for separating a sperm sub-population of interest from a semen sample from a non-human animal by providing a density gradient comprising at least two layers of the composition of the invention, each layer having a different density; separating the sperm sub-populations in the semen sample by centrifugation through the density gradient; and selecting the sperm sub-population of interest. Finally, spermatozoa prepared by these methods and the use of such spermatozoa in artificial insemination, in vitro fertilisation or intracytoplasmic sperm injection.
    • 用于加工动物精液用于辅助生殖的组合物和方法。 用于从精液样品中分离精子的组合物或胶体制剂,所述组合物包含至少一种碱金属和/或碱土金属盐,EDTA,两性离子缓冲液,硅烷涂覆的二氧化硅颗粒和水, 组合物的pH为7.0-7.35,渗透压为300-345mOsm。 从非人类动物的精液样品中通过通过单层组合物或胶体制剂离心分离精子与其他精液成分来制备精子的方法。 另外,通过提供包含本发明组合物的至少两层的密度梯度,每层具有不同的密度,分离来自非人类动物的精液样本的目的精子亚群的方法; 通过密度梯度离心分离精液样本中的精子亚群; 并选择感兴趣的精子亚群体。 最后,通过这些方法制备的精子和使用这样的精子进行人工授精,体外受精或细胞质内精子注射。
    • 3. 发明申请
    • COMPOSITION FOR SEPARATING SPERMATOZOA FROM A SEMEN SAMPLE
    • 用于从SEMENA样品分离SPERMATOZOA的组合物
    • US20110004051A1
    • 2011-01-06
    • US12677034
    • 2008-09-05
    • Jane MorrellHeriberto Rodriguez-Martinez
    • Jane MorrellHeriberto Rodriguez-Martinez
    • A61B17/43C12N5/076
    • C12N1/02A61K35/52C12N5/061C12N5/0612
    • Compositions and methods useful for processing animal semen for use in assisted reproduction. A composition, or colloid formulation, for separation of spermatozoa from a semen sample, the composition including at least one salt of an alkali metal and/or an alkaline earth metal, EDTA, a zwitterion buffer, silane-coated silica particles and water, the composition having a pH of 7.0-7.35 and an osmolarity of 300-345 mOsm. A method for preparing spermatozoa from a semen sample from a non-human animal by separating the spermatozoa from other semen constituents by centrifugation through a single layer of the composition, or colloid formulation. Also, a method for separating a sperm sub-population of interest from a semen sample from a non-human animal by providing a density gradient comprising at least two layers of the composition of the invention, each layer having a different density; separating the sperm sub-populations in the semen sample by centrifugation through the density gradient; and selecting the sperm sub-population of interest. Finally, spermatozoa prepared by these methods and the use of such spermatozoa in artificial insemination, in vitro fertilisation or intracytoplasmic sperm injection.
    • 用于加工动物精液用于辅助生殖的组合物和方法。 用于从精液样品中分离精子的组合物或胶体制剂,所述组合物包含至少一种碱金属和/或碱土金属盐,EDTA,两性离子缓冲液,硅烷涂覆的二氧化硅颗粒和水, 组合物的pH为7.0-7.35,渗透压为300-345mOsm。 从非人类动物的精液样品中通过通过单层组合物或胶体制剂离心分离精子与其他精液成分来制备精子的方法。 另外,通过提供包含本发明组合物的至少两层的密度梯度,每层具有不同的密度,分离来自非人类动物的精液样本的目的精子亚群的方法; 通过密度梯度离心分离精液样本中的精子亚群; 并选择感兴趣的精子亚群体。 最后,通过这些方法制备的精子和使用这样的精子进行人工授精,体外受精或细胞质内精子注射。