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1. 发明申请

US20050064596A1 Buffer solution for electroporation and a method comprising the use of the same 有权
标题翻译：用于电穿孔的缓冲溶液和包括其使用的方法
公开(公告)号：US20050064596A1
公开(公告)日：2005-03-24
申请号：US10475967
申请日：2002-04-23
申请人： Gudula Riemen , Elke Lorbach , Juliana Helfrich , Gregor Siebenkotten , Herbert Muller-Hartmann , Kirsten Rothmann-Cosic , Corinna Thiel , Meike Weigel , Heike Wessendorf , Helmut Brosterbus , Michael Nix
发明人： Gudula Riemen , Elke Lorbach , Juliana Helfrich , Gregor Siebenkotten , Herbert Muller-Hartmann , Kirsten Rothmann-Cosic , Corinna Thiel , Meike Weigel , Heike Wessendorf , Helmut Brosterbus , Michael Nix
IPC分类号： C12N15/09 , A61K35/12 , A61K38/00 , A61K47/00 , A61K48/00 , C12N13/00 , C12N15/87 , G01N33/50
CPC分类号： C12N15/87 , A61K38/00 , A61K48/00
摘要： The invention relates to a buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce said biologically active molecules into the cells using an electric current and to a method for introducing biologically active molecules into animal or human cells using an electric current and a buffer solution. The inventive buffer solution has a buffering capacity of at least 20 mmol*I−1*pH−1 and an ionic strength of at least 200 mmol*I−1 during a change to the pH value from pH 7 to pH 8 and at a temperature of 25° C. The use of a buffer solution of this type in the corresponding method allows biologically active molecules to be introduced into animal and human cells with a high degree of transfection efficiency and at the same time a low cell mortality. Different cell types, in particular dormant and actively dividing cells of low activity, can be successfully transfected in said buffer solution.
摘要翻译：本发明涉及一种用于悬浮动物或人细胞并用于溶解生物活性分子以便使用电流将所述生物活性分子引入细胞的缓冲溶液以及使用以下方法将生物活性分子引入动物或人细胞的方法：电流和缓冲液。本发明的缓冲溶液在pH值从pH7变化期间具有至少20mmol * I -1 * pH <-1的缓冲能力和至少200mmol * I -1的离子强度至pH8，温度为25℃。在相应方法中使用这种类型的缓冲溶液使生物活性分子以高度的转染效率被引入动物和人类细胞，并且同时细胞死亡率低。可以在所述缓冲溶液中成功转染不同细胞类型，特别是低活性的休眠和活跃分化细胞。

2. 发明申请

US20080145938A1 BUFFER SOLUTION FOR ELECTROPORATION AND A METHOD COMPRISING THE USE OF THE SAME 审中-公开
标题翻译：用于电镀的缓冲溶液和包含其使用的方法
公开(公告)号：US20080145938A1
公开(公告)日：2008-06-19
申请号：US11931886
申请日：2007-10-31
申请人： Gudula Riemen , Elke Lorbach , Juliana Helfrich , Gregor Siebenkotten , Herbert Muller-Hartmann , Kirsten Rothmann-Cosic , Corinna Thiel , Meike Weigel , Heike Wessendorf , Helmut Brosterbus , Michael Nix
发明人： Gudula Riemen , Elke Lorbach , Juliana Helfrich , Gregor Siebenkotten , Herbert Muller-Hartmann , Kirsten Rothmann-Cosic , Corinna Thiel , Meike Weigel , Heike Wessendorf , Helmut Brosterbus , Michael Nix
IPC分类号： C12N15/87 , C12N5/06
CPC分类号： C12N15/87 , A61K38/00 , A61K48/00
摘要： A buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce the biologically active molecules into the cells using electric current. The buffer solution includes HEPES and at least 10 mmol*1−1 magnesium ions (Mg2+). The buffer solution has a buffer capacity of at least 20 mmol*1−1*pH−1 at a change in the pH from pH 7 to pH 8 and at a temperature of 25° C. The buffer solution also has an ionic strength of at least 200 mmol*1−1.
摘要翻译：用于悬浮动物或人细胞并溶解生物活性分子以便使用电流将生物活性分子引入细胞的缓冲溶液。缓冲溶液包括HEPES和至少10mmol * 1-1的镁离子（Mg 2+）。在pH7至pH8的pH变化下，缓冲溶液的缓冲容量至少为20mmol * 1-pH * pH 1〜的缓冲溶液的离子强度至少为200毫摩尔/升。

3. 发明申请

US20080113436A1 BUFFER SOLUTION FOR ELECTROPORATION AND A METHOD COMPRISING THE USE OF THE SAME 有权
标题翻译：用于电镀的缓冲溶液和包含其使用的方法
公开(公告)号：US20080113436A1
公开(公告)日：2008-05-15
申请号：US11931996
申请日：2007-10-31
申请人： Gudula Riemen , Elke Lorbach , Juliana Helfrich , Gregor Siebenkotten , Herbert Muller-Hartmann , Kirsten Rothmann-Cosic , Corinna Thiel , Meike Weigel , Heike Wessendorf , Helmut Brosterbus , Michael Nix
发明人： Gudula Riemen , Elke Lorbach , Juliana Helfrich , Gregor Siebenkotten , Herbert Muller-Hartmann , Kirsten Rothmann-Cosic , Corinna Thiel , Meike Weigel , Heike Wessendorf , Helmut Brosterbus , Michael Nix
IPC分类号： C12N15/87 , C12N5/06 , C12N13/00
CPC分类号： C12N15/87 , A61K38/00 , A61K48/00
摘要： A buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce said biologically active molecules into the cells using electric current. The buffer solution includes at least one of sodium succinate, mannitol and sodium lactobionate. The buffer solution has a buffer capacity of at least 20 mmol*l−1*pH−1 at a change in the pH from pH 7 to pH 8 and at a temperature of 25° C., and an ionic strength of at least 200 mmol*l−1.
摘要翻译：用于悬浮动物或人细胞并溶解生物活性分子以便使用电流将所述生物活性分子引入细胞的缓冲溶液。缓冲溶液包括琥珀酸钠，甘露糖醇和乳糖酸钠中的至少一种。在pH7至pH8的pH变化下，缓冲溶液的缓冲容量至少为20mmol·l -1·pH-1〜 25℃的离子强度和至少200mmol / l的离子强度。

4. 发明申请

US20060087522A1 Container and device for generating electric fields in different chambers 有权
标题翻译：用于在不同室内产生电场的容器和装置
公开(公告)号：US20060087522A1
公开(公告)日：2006-04-27
申请号：US11079192
申请日：2005-03-15
申请人： Herbert Muller-Hartmann , Michael Habig
发明人： Herbert Muller-Hartmann , Michael Habig
IPC分类号： B41J2/01
CPC分类号： C12M23/12 , B01J19/0046 , B01J2219/00315 , B01J2219/00317 , B01J2219/00585 , B01J2219/00599 , B01J2219/00689 , B01J2219/00713 , B01J2219/00743 , B01L3/50855 , B01L2300/0645 , B01L2300/0829 , C12M35/02
摘要： The invention concerns a container 1 with chambers 2 which each comprise at least one pair of electrodes including a first 4 and a second electrode 5 for the application of electric voltage for generating an electric field within one chamber 2. At least two first electrodes 4 of different chambers 3 are conductively coupled and at least one second electrode 5 of said chamber 2 is separately conductively connectable. The invention further concerns a method for manufacturing said container 1 as well as a device for electrically contacting at least one of said containers 1.
摘要翻译：本发明涉及具有腔室2的容器1，每个腔室2包括至少一对电极，其包括用于在一个腔室2内产生电场的电压施加的第一电极5和第二电极5。不同腔室3的至少两个第一电极4被导电耦合，并且所述腔室2的至少一个第二电极5可分开导电连接。本发明还涉及用于制造所述容器1的方法以及用于电接触所述容器1中的至少一个的装置。

5. 发明申请

US20090023131A1 CIRCUIT ARRANGEMENT FOR INJECTING NUCLEIC ACIDS AND OTHER BIOLOGICALLY ACTIVE MOLECULES INTO THE NUCLEUS OF HIGHER EUCARYONTIC CELLS USING ELECTRICAL CURRENT 审中-公开
标题翻译：使用电流将核酸和其他生物活性分子注入高核细胞核的电路布置
公开(公告)号：US20090023131A1
公开(公告)日：2009-01-22
申请号：US11836656
申请日：2007-08-09
申请人： Herbert Muller-Hartmann , Gudula Riemen , Kirsten Rothmann-Cosic , Corinna Thiel , Ludger Altrogge , Meike Weigel , Rainer Christine , Elke Lorbach , Juliana Helfrich , Heike Wessendorf , Gregor Siebenkotten
发明人： Herbert Muller-Hartmann , Gudula Riemen , Kirsten Rothmann-Cosic , Corinna Thiel , Ludger Altrogge , Meike Weigel , Rainer Christine , Elke Lorbach , Juliana Helfrich , Heike Wessendorf , Gregor Siebenkotten
IPC分类号： C12Q3/00
CPC分类号： A61N1/327 , A61N1/0412 , C12N15/87 , H03K3/57
摘要： The invention relates to a novel circuit arrangement for electrotransfection or electrofusion, which enables the transportation of DNA and/or other biologically active molecules to the nucleus of higher eukaryotic cells or the fusion of cells, independent of cell division and with reduced cell mortality.
摘要翻译：本发明涉及一种用于电转移或电融合的新型电路装置，其能够将DNA和/或其他生物活性分子运输到高等真核细胞的细胞核或细胞融合，而与细胞分裂无关并具有降低的细胞死亡率。

6. 发明申请

US20070110736A1 Buffer solution for electroporation and a method comprising the use of the same 有权
标题翻译：用于电穿孔的缓冲溶液和包括其使用的方法
公开(公告)号：US20070110736A1
公开(公告)日：2007-05-17
申请号：US11595448
申请日：2006-11-09
申请人： Gudula Riemen , Elke Lorbach , Juliana Helfrich , Gregor Siebenkotten , Herbert Muller-Hartmann , Kirsten Rothmann-Cosic , Corinna Thiel , Meike Weigel , Heike Wessendorf , Helmut Brosterhus , Michael Nix
发明人： Gudula Riemen , Elke Lorbach , Juliana Helfrich , Gregor Siebenkotten , Herbert Muller-Hartmann , Kirsten Rothmann-Cosic , Corinna Thiel , Meike Weigel , Heike Wessendorf , Helmut Brosterhus , Michael Nix
IPC分类号： A61K48/00 , C12N5/08 , C12N15/09 , C12N15/87
CPC分类号： C12N15/87 , A61K38/00 , A61K48/00
摘要： The invention relates to a buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce said biologically active molecules into the cells using an electric current and to a method for introducing biologically active molecules into animal or human cells using an electric current and a buffer solution. The inventive buffer solution has a buffering capacity of at least 20 mmol*I−1*pH−1 and an ionic strength of at least 200 mmol* I−1 during a change to the pH value from pH 7 to pH 8 and at a temperature of 25 ° C. The use of a buffer solution of this type in the corresponding method allows biologically active molecules to be introduced into animal and human cells with a high degree of transfection efficiency and at the same time a low cell mortality. Different cell types, in particular dormant and actively dividing cells of low activity, can be successfully transfected in said buffer solution.
摘要翻译：本发明涉及一种用于悬浮动物或人细胞并用于溶解生物活性分子以便使用电流将所述生物活性分子引入细胞的缓冲溶液以及使用以下方法将生物活性分子引入动物或人细胞的方法：电流和缓冲液。本发明缓冲溶液的缓冲能力至少为20毫摩尔/升，pH为-1，离子强度为至少200毫摩尔/升，在pH值从pH7变化到pH8至25℃的温度下，在1℃下使用。在相应方法中使用这种类型的缓冲溶液允许将生物活性分子引入动物和人细胞具有高转染效率，同时细胞死亡率低。可以在所述缓冲溶液中成功转染不同细胞类型，特别是低活性的休眠和活跃分化细胞。

7. 发明申请

US20060160221A1 Device and method for processing biological material 有权
标题翻译：用于处理生物材料的装置和方法
公开(公告)号：US20060160221A1
公开(公告)日：2006-07-20
申请号：US10527958
申请日：2003-09-12
申请人： Gregor Siebenkotten , Herbert Muller-Hartmann , Gudula Riemen , Gunter Kraus
发明人： Gregor Siebenkotten , Herbert Muller-Hartmann , Gudula Riemen , Gunter Kraus
IPC分类号： C12N15/87 , C12M1/42
CPC分类号： C12M35/02 , C12N13/00
摘要： The invention relates to a device for processing biological material, which at least comprises a chamber which at least can be closed in relation to the outside and which comprises an inner space for receiving said biological material, wherein said chamber comprises at least one electrode which is placed in contact with said inner space of said chamber and is provided for generating an electric field. The invention also relates to a method for processing biological material, wherein said biological material is introduced into the inner space of a chamber which at least can be closed in relation to the outside and which comprises at least one electrode which is placed in contact with said inner space of said chamber and is provided for generating an electric field which is generated in said inner space after introducing said biological material by applying voltage to said electrode and a further electrode which is in contact with said inner space of said chamber. According to the invention, said chamber comprises at least one inlet line which comprises at least one opening arranged close to said electrode. According to the inventive method, said biological material is almost completely rinsed out of said inner space of said chamber by means of a solution after said electric field is generated, said solution being guided via an inlet line of said chamber along at least one electrode.
摘要翻译：本发明涉及一种用于处理生物材料的装置，其至少包括至少可以相对于外部封闭的室，并且其包括用于接收所述生物材料的内部空间，其中所述室包括至少一个电极，与所述室的所述内部空间接触并且被设置用于产生电场。本发明还涉及一种用于处理生物材料的方法，其中所述生物材料被引入至少可以相对于外部封闭的室的内部空间中，并且包括至少一个与所述的所述室的内部空间被设置用于在通过向所述电极施加电压而引入所述生物材料之后产生在所述内部空间中产生的电场，以及与所述室的所述内部空间接触的另外的电极。根据本发明，所述腔室包括至少一个入口管线，其包括靠近所述电极布置的至少一个开口。根据本发明的方法，所述生物材料在所述电场产生之后通过溶液几乎完全从所述室的所述内部空间中漂洗出来，所述溶液沿着至少一个电极经由所述室的入口引导。

8. 发明申请

US20050214562A1 Method for generating an elecrically contactable area on a doped polymer and formed body produced by this method 有权
标题翻译：用于通过该方法产生的掺杂聚合物和成形体上产生可电接触区域的方法
公开(公告)号：US20050214562A1
公开(公告)日：2005-09-29
申请号：US10972294
申请日：2004-10-25
申请人： Herbert Muller-Hartmann , Ewald Fernbach , Gregor Siebenkotten
发明人： Herbert Muller-Hartmann , Ewald Fernbach , Gregor Siebenkotten
IPC分类号： A61N1/30 , A61N1/32 , C12M1/42 , H01C1/14 , H01C1/142 , C04B35/00
CPC分类号： C12M35/02 , A61N1/0408 , A61N1/0472 , A61N1/0496 , A61N1/30 , A61N1/327 , H01C1/142 , Y10T156/1039 , Y10T428/12493 , Y10T428/12569
摘要： The invention relates to a method for generating at least one electrically contactable area on a polymer which is doped with a conductive substance, wherein a contact material is applied onto the polymer, which has a lower specific resistance at 23° C. than the polymer. According to the invention the contact material is applied onto the polymer so tightly that close contact between the contact material and the conductive substance is achieved. Due to the tight application of the contact material, which has a lower specific resistance than the polymer, the input resistance of the doped polymer is effectively reduced. The invention further concerns a formed body made of a polymer which is doped with a conductive substance, which has at least one contactable area, within which a contact material is applied onto the polymer, which has a lower specific resistance at 23° C. than the polymer. According to the invention the contact material is applied onto the polymer so tightly that it is in close contact to the conductive substance. Such formed body has a significantly reduced input resistance.
摘要翻译：本发明涉及一种用于在掺杂有导电物质的聚合物上产生至少一个电接触区域的方法，其中将接触材料施加到聚合物上，该聚合物在23℃比聚合物具有较低的电阻率。根据本发明，接触材料被紧密地涂覆到聚合物上，从而实现了接触材料和导电物质之间的紧密接触。由于具有比聚合物低的电阻率的接触材料的紧密应用，有效地降低了掺杂聚合物的输入电阻。本发明还涉及一种由聚合物制成的成形体，该聚合物掺杂有导电物质，该导电物质具有至少一个可接触区域，在该接触区域内将接触材料施加到聚合物上，该聚合物在23℃具有较低的电阻率，聚合物。根据本发明，接触材料如此牢固地施加到聚合物上，使得其与导电物质紧密接触。这种成形体具有显着降低的输入电阻。

9. 发明申请

US20050064578A1 Container with at least one electrode 有权
标题翻译：容器至少有一个电极
公开(公告)号：US20050064578A1
公开(公告)日：2005-03-24
申请号：US10505149
申请日：2003-02-20
申请人： Herbert Muller-Hartmann , Michael Habig , Peter Hoffmann , Gregor Siebenkotten
发明人： Herbert Muller-Hartmann , Michael Habig , Peter Hoffmann , Gregor Siebenkotten
IPC分类号： C12N15/09 , C12M1/00 , C12M1/42 , C12M3/00 , C12N5/10
CPC分类号： C12M35/02
摘要： The invention relates to a container 20, 30 for receiving an aqueous solution, which is formed at least partially by an outer limit 21 forming an inner chamber 22, 32 for receiving the solution, and which comprises at least one area which acts as an electrode 25, 26, 33, 34 when an electric voltage is applied and a subsequent discharge occurs, wherein at least one electrode 25, 26, 33, 34 is made of a conductive synthetic material at least based on a plastic material which is doped with at least one conductive substance. A container 20, 30 of the above-mentioned kind is created this way, which is simple and economical to produce and also, for example, enables an efficient transfection of living cells by means of electroporation or an effective electrofusion.
摘要翻译：本发明涉及用于接收水溶液的容器20,30，其至少部分地由形成用于接收溶液的内室22,32的外限制21形成，并且包括至少一个充当电极的区域 25,26,33,34，当施加电压并发生随后的放电时，其中至少一个电极25,26,33,34由导电合成材料制成，至少基于掺杂在其上的塑料材料至少一种导电物质。以这种方式产生上述类型的容器20,30，其生产简单和经济，并且还例如能够通过电穿孔或有效的电融合有效转染活细胞。

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