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1. 发明专利

IL251438D0 Method for peptide synthesis and apparatus for carrying out a method for solid phase synthesis of peptides 未知
公开(公告)号：IL251438D0
公开(公告)日：2017-05-29
申请号：IL25143817
申请日：2017-03-28
申请人： SULFOTOOLS GMBH , SASCHA KNAUER , TOBIAS MICHAEL LOUIS ROESE , OLGA AVRUTINA , HARALD KOLMAR , CHRISTINA UTH
发明人： SASCHA KNAUER , TOBIAS MICHAEL LOUIS ROESE , OLGA AVRUTINA , HARALD KOLMAR , CHRISTINA UTH
IPC分类号： C07K20060101
摘要： The invention relates to a method for peptide synthesis, wherein said method comprises the steps of reacting a first amino acid or a first peptide with an α-amine protected second amino acid in a solvent selected from the group consisting of water, alcohol, and a mixture of water and alcohol, and removing the α-amine protecting group with a deprotecting solution. The invention further relates to protective agents, their use and an apparatus for carrying out a method for solid phase synthesis of peptides.

2. 发明授权

US08258258B2 Dimeric or multimeric microproteins 有权
标题翻译：二聚体或多聚体微蛋白
公开(公告)号：US08258258B2
公开(公告)日：2012-09-04
申请号：US11886007
申请日：2006-03-09
申请人： Harald Kolmar , Ernst Boehnlein , Alexander Wentzel , Hans-Ulrich Schmoldt
发明人： Harald Kolmar , Ernst Boehnlein , Alexander Wentzel , Hans-Ulrich Schmoldt
IPC分类号： A61K38/16
CPC分类号： C07K14/00 , A61K38/00
摘要： Disclosed is a polypeptide comprising at least two microproteins, which preferably comprise an amino acid sequence having a specific binding activity to a target protein. Furthermore, disclosed are polynucleotides encoding such a polypeptide as well as pharmaceutical compositions and kits comprising said polypeptide or polynucleotide. Also disclosed herein are methods of treatments and second medical uses applying the disclosed polypeptide or polynucleotide. Additionally, the disclosure of the present application relates to a method for forming a covalent bond in a microprotein which can be used for producing the disclosed polypeptides.
摘要翻译：公开了包含至少两种微蛋白的多肽，其优选包含对靶蛋白具有特异性结合活性的氨基酸序列。此外，公开了编码这种多肽的多核苷酸以及包含所述多肽或多核苷酸的药物组合物和试剂盒。本文还公开了应用所公开的多肽或多核苷酸的治疗方法和第二医疗用途。此外，本申请的公开内容涉及在微蛋白中形成共价键的方法，其可用于制备所公开的多肽。

3. 发明申请

US20090130692A1 Use of microproteins as tryptase 失效
标题翻译：使用微蛋白作为类胰蛋白酶
公开(公告)号：US20090130692A1
公开(公告)日：2009-05-21
申请号：US11663435
申请日：2005-09-19
申请人： Harald Kolmar , Christian Sommerhoff , Alexander Wentzel
发明人： Harald Kolmar , Christian Sommerhoff , Alexander Wentzel
IPC分类号： G01N33/567 , C07K14/00 , C07H21/00 , C12N9/24 , C07H21/04 , C12N5/10 , C12Q1/34 , A61K38/47 , C12P21/02 , C12N15/63 , A61K35/12 , A61K31/7088 , A61K38/16
CPC分类号： A61K38/56 , C07K14/47 , C07K2319/00 , C30B7/00 , C30B29/58
摘要： Disclosed are uses of microproteins preferably microproteins forming a cystine knot (i.e. belonging to the family of inhibitor cystine knot (ICK) polypeptides) or polynucleotides encoding said microproteins for the preparation of a pharmaceutical composition for treating or preventing a disease that can be treated or prevented by inhibiting the activity of tryptase as well as corresponding methods of treatment. Also disclosed are uses of the microproteins for inhibiting tryptase activity, for purifying tryptase, as a carrier molecule for tryptase and for deleting or quantifying tryptase in a sample, including corresponding diagnostic applications. Furthermore disclosed are fusion proteins comprising an inactive barnase as well as fusion proteins comprising barnase and a microprotein. Also encompassed are nucleic acid molecules encoding such a fusion protein, as well as corresponding vectors, host cells, preparation methods and uses of the fusion protein. Moreover, the present application discloses a crystal of a microprotein fused with barnase, preferably inactive barnase. The disclosure also refers to corresponding preparation methods for the crystal, structure analysis methods using the crystal data storage media comprising the structure data obtained, as well as to in silico methods using the structure data for characterizing the binding of microproteins to target molecules. Furthermore, disclosed are pharmaceutical compositions comprising the crystal and corresponding medical uses.
摘要翻译：公开了微型蛋白质的使用，优选微型蛋白质形成胱氨酸结（即属于抑制剂胱氨酸结（ICK）多肽家族）或编码所述微蛋白的多核苷酸用于制备用于治疗或预防可被治疗或预防的疾病的药物组合物通过抑制类胰蛋白酶的活性以及相应的治疗方法。还公开了微蛋白用于抑制类胰蛋白酶活性，纯化类胰蛋白酶的作用，作为类胰蛋白酶的载体分子以及删除或定量样品中的类胰蛋白酶的用途，包括相应的诊断应用。此外，还公开了包含非活性芽孢杆菌RNA酶的融合蛋白以及包含芽孢杆菌RNA酶和微蛋白的融合蛋白。还包括编码这种融合蛋白的核酸分子，以及相应的载体，宿主细胞，制备方法和融合蛋白的用途。此外，本申请公开了一种与芽孢杆菌酶融合的微蛋白的晶体，优选不活泼的芽孢杆菌RNA酶。本公开还涉及晶体的相应制备方法，使用包含所获得的结构数据的晶体数据存储介质的结构分析方法，以及使用结构数据表征微结构蛋白与靶分子的结合的计算机方法。此外，公开了包含晶体和相应医疗用途的药物组合物。

4. 发明授权

US07186524B2 Method for exposing peptides and polypeptides on the cell surface of bacteria 失效
标题翻译：在细菌细胞表面上暴露肽和多肽的方法
公开(公告)号：US07186524B2
公开(公告)日：2007-03-06
申请号：US10415165
申请日：2001-10-25
申请人： Harald Kolmar , Andreas Christmann , Alexander Wentzel
发明人： Harald Kolmar , Andreas Christmann , Alexander Wentzel
IPC分类号： C12P21/06 , C12N1/20 , C12N15/00
CPC分类号： C12N15/70 , C07K14/245 , C07K2319/00 , C12N11/16
摘要： The inventive method allows peptides or polypeptides to be exposed on the surface of gram-negative host bacteria using specific intimin-based anchor modules. Intimins with shortened carboxy terminals have been found to be particularly suitable anchor modules for passenger domains in the exterior E. coli cell membrane. According to the method, host bacteria are transformed using vectors, on which are located a fused nucleic acid sequence consisting of a sequence segment which codes for an intimin with a shortened carboxy terminal and a nucleic acid sequence segment which codes for the passenger peptide that is to be exposed. The invention permits a particularly large number of passenger domains to be exposed on the cell surface of the bacteria, without adversely affecting the viability of the bacteria.
摘要翻译：本发明的方法允许使用特定的基于Intimin的锚模块将肽或多肽暴露在革兰氏阴性宿主细菌的表面上。已经发现具有缩短的羧基末端的内皮素特别适用于外部大肠杆菌细胞膜中的载体结构域的锚模块。根据该方法，使用载体转化宿主细菌，其中位于由编码具有缩短的羧基末端的肠溶蛋白的序列区段和由序列片段编码的载体肽所构成的核酸序列片段的融合核酸序列被暴露。本发明允许特别大量的乘客区域暴露在细菌的细胞表面上，而不会不利地影响细菌的存活力。

5. 发明授权

US5882924A Vectors for the genetic selection of ligand binding proteins in microorganisms by means of signal transduction 失效
标题翻译：通过信号转导在微生物中遗传选择配体结合蛋白的载体
公开(公告)号：US5882924A
公开(公告)日：1999-03-16
申请号：US956047
申请日：1997-10-22
申请人： Hans-Joachim Fritz , Frank Hennecke , Harald Kolmar
发明人： Hans-Joachim Fritz , Frank Hennecke , Harald Kolmar
IPC分类号： C12N1/21 , C07K14/28 , C07K16/44 , C12N15/09 , C12N15/62 , C12N15/64 , C12R1/19 , C12N15/11 , C12N15/70 , C12Q1/68
CPC分类号： C07K16/44 , C07K14/28 , C07K2317/622 , C07K2319/00
摘要： The invention relates to a process for the genetic selection in microorganisms of proteins which are capable of ligand binding, in which process a protein which is capable of ligand binding is presented extracytoplasmically and the signal of the ligand binding is passed on by signal transduction to the biosynthetic machinery of the micoorganism for the purpose of expressing a detectable and/or selectable function. In addition to this, the patent discloses microorganisms which are suitable for use in this process, as well as replicons and processes for their preparation.
摘要翻译：本发明涉及一种能够进行配体结合的蛋白质微生物遗传选择的方法，其中能够配体结合的蛋白质可以在胞外存在，并且配体结合的信号通过信号转导传递给用于表达可检测和/或可选功能的微生物的生物合成机制。除此之外，该专利公开了适用于该方法的微生物以及其制备的复制子和方法。

6. 发明授权

US08278262B2 Use of microproteins as tryptase inhibitors 失效
标题翻译：使用微蛋白作为类胰蛋白酶抑制剂
公开(公告)号：US08278262B2
公开(公告)日：2012-10-02
申请号：US11663435
申请日：2005-09-19
申请人： Harald Kolmar , Christian Sommerhoff , Alexander Wentzel
发明人： Harald Kolmar , Christian Sommerhoff , Alexander Wentzel
IPC分类号： A61K38/00 , G01N33/567
CPC分类号： A61K38/56 , C07K14/47 , C07K2319/00 , C30B7/00 , C30B29/58
摘要： Disclosed are uses of microproteins preferably microproteins forming a cystine knot (i.e. belonging to the family of inhibitor cystine knot (ICK) polypeptides) or polynucleotides encoding said microproteins for the preparation of a pharmaceutical composition for treating or preventing a disease that can be treated or prevented by inhibiting the activity of tryptase as well as corresponding methods of treatment. Also disclosed are uses of the microproteins for inhibiting tryptase activity, for purifying tryptase, as a carrier molecule for tryptase and for deleting or quantifying tryptase in a sample, including corresponding diagnostic applications. Furthermore disclosed are fusion proteins comprising an inactive barnase as well as fusion proteins comprising barnase and a microprotein. Also encompassed are nucleic acid molecules encoding such a fusion protein, as well as corresponding vectors, host cells, preparation methods and uses of the fusion protein. Moreover, the present application discloses a crystal of a microprotein fused with barnase, preferably inactive barnase. The disclosure also refers to corresponding preparation methods for the crystal, structure analysis methods using the crystal data storage media comprising the structure data obtained, as well as to in silico methods using the structure data for characterizing the binding of microproteins to target molecules. Furthermore, disclosed are pharmaceutical compositions comprising the crystal and corresponding medical uses.
摘要翻译：公开了微型蛋白质的使用，优选微型蛋白质形成胱氨酸结（即属于抑制剂胱氨酸结（ICK）多肽家族）或编码所述微蛋白的多核苷酸用于制备用于治疗或预防可被治疗或预防的疾病的药物组合物通过抑制类胰蛋白酶的活性以及相应的治疗方法。还公开了微蛋白用于抑制类胰蛋白酶活性，纯化类胰蛋白酶的作用，作为类胰蛋白酶的载体分子以及删除或定量样品中的类胰蛋白酶的用途，包括相应的诊断应用。此外，还公开了包含非活性芽孢杆菌RNA酶的融合蛋白以及包含芽孢杆菌RNA酶和微蛋白的融合蛋白。还包括编码这种融合蛋白的核酸分子，以及相应的载体，宿主细胞，制备方法和融合蛋白的用途。此外，本申请公开了一种与芽孢杆菌酶融合的微蛋白的晶体，优选不活泼的芽孢杆菌RNA酶。本公开还涉及晶体的相应制备方法，使用包含所获得的结构数据的晶体数据存储介质的结构分析方法，以及使用结构数据表征微结构蛋白与靶分子的结合的计算机方法。此外，公开了包含晶体和相应医疗用途的药物组合物。

7. 发明申请

US20100267610A1 Polypeptide comprising a knottin protein moiety 审中-公开
标题翻译：包含knottin蛋白部分的多肽
公开(公告)号：US20100267610A1
公开(公告)日：2010-10-21
申请号：US12527201
申请日：2008-02-18
申请人： Michael Blind , Harald Kolmar
发明人： Michael Blind , Harald Kolmar
IPC分类号： A61K38/02 , C07K2/00 , A61P19/10
CPC分类号： C07K14/8139 , A61K38/00 , C07K14/635 , C07K2319/00
摘要： A polypeptide, with an amino acid sequence different from that of knottin protein, having a scaffold moiety with a helix moiety inserted in the scaffold moiety is described. The scaffold moiety comprises a knottin protein, or a fragment of knottin protein.
摘要翻译：描述了具有不同于knottin蛋白的氨基酸序列的多肽，其具有插入支架部分中的螺旋部分的支架部分。支架部分包含knottin蛋白或knottin蛋白的片段。

8. 发明申请

US20090156476A1 Dimeric or Multimeric Microproteins 有权
标题翻译：二聚体或多聚体微蛋白
公开(公告)号：US20090156476A1
公开(公告)日：2009-06-18
申请号：US11886007
申请日：2006-03-09
申请人： Harald Kolmar , Ernst Boehnlein , Alexander Wentzel , Hans-Ulrich Schmoldt
发明人： Harald Kolmar , Ernst Boehnlein , Alexander Wentzel , Hans-Ulrich Schmoldt
IPC分类号： A61K38/16 , C07K2/00 , C07K14/00 , C12N15/11 , A61K31/7088 , C12N9/99
CPC分类号： C07K14/00 , A61K38/00
摘要： Disclosed is a polypeptide comprising at least two microproteins, which preferably comprise an amino acid sequence having a specific binding activity to a target protein. Furthermore, disclosed are polynucleotides encoding such a polypeptide as well as pharmaceutical compositions and kits comprising said polypeptide or polynucleotide. Also disclosed herein are methods of treatments and second medical uses applying the disclosed polypeptide or polynucleotide. Additionally, the disclosure of the present application relates to a method for forming a covalent bond in a microprotein which can be used for producing the disclosed polypeptides.
摘要翻译：公开了包含至少两种微蛋白的多肽，其优选包含对靶蛋白具有特异性结合活性的氨基酸序列。此外，公开了编码这种多肽的多核苷酸以及包含所述多肽或多核苷酸的药物组合物和试剂盒。本文还公开了应用所公开的多肽或多核苷酸的治疗方法和第二医疗用途。此外，本申请的公开内容涉及在微蛋白中形成共价键的方法，其可用于制备所公开的多肽。

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