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1. 发明申请

WO2008045852A3 PURIFICATION OF STREPTOCOCCUS PNEUMONIAE TYPE 3 POLYSACCHARIDES 审中-公开
标题翻译：。US E E。。。。。。。。。。。。。。。。。。
公开(公告)号：WO2008045852A3
公开(公告)日：2008-06-19
申请号：PCT/US2007080768
申请日：2007-10-09
申请人： WYETH CORP , BAHLER BRIAN DOUGLAS , HUGHES ERIK HELLER , LEE TSU-SHUN
发明人： BAHLER BRIAN DOUGLAS , HUGHES ERIK HELLER , LEE TSU-SHUN
IPC分类号： C12P19/04 , A61K31/715 , A61P31/04
CPC分类号： C12P19/04 , A61K31/715 , Y10S424/831
摘要： The present invention provides improved methods for the reduction or removal of protein impurities from a complex cellular Streptococcus pneumoniae lysate or centrate comprising serotype 3 polysaccharides involving steps relating to post-lysis heating or pH adjustment. In certain methods, the lysate is heated for a time and at a temperature sufficient to denature proteins present in the lysate and cause their aggregation and precipitation. In one embodiment, the lysate is heated to at least 60°C for at least 30 minutes to cause protein aggregation and precipitation, more particularly about 60°C to about 70°C for about 30 to about 50 minutes, and even more particularly about 65°C for about 40 minutes. In other methods, the pH of the lysate or centrate is increased to at least 8.0 to improve filterability, more particularly about 8.0 to 8.4, and even more particularly about 8.2. In further methods, heating and pH adjustment steps are combined to cause the aggregation and precipitation of proteins as well as to improve filterability of the lysates or centrates. In other methods, the pH of the lysate or centrate is lowered to about 3.0 to about 5.0 to cause protein aggregation and precipitation. Such methods allow for the production of substantially purified serotype 3 polysaccharide-containing lysates or centrates.
摘要翻译：本发明提供了用于从复合细胞性肺炎链球菌裂解物或浓缩物中还原或除去蛋白质杂质的改进方法，其包含涉及裂解加热或pH调节的步骤的血清型3多糖。在某些方法中，将裂解物加热一段时间并且在足以使存在于裂解物中的蛋白质变性并导致其聚集和沉淀的温度下加热。在一个实施方案中，将裂解物加热至至少60℃至少30分钟以引起蛋白质聚集和沉淀，更特别地约60℃至约70℃约30至约50分钟，甚至更特别地约为 65°C约40分钟。在其他方法中，将裂解物或浓缩物的pH增加至至少8.0以提高过滤性，更特别地约8.0至8.4，甚至更特别约8.2。在进一步的方法中，加热和pH调节步骤相结合以引起蛋白质的聚集和沉淀以及提高裂解物或浓缩物的过滤性。在其他方法中，裂解物或浓缩物的pH降低至约3.0至约5.0以引起蛋白质聚集和沉淀。这样的方法允许生产基本上纯化的血清型3多糖裂解物或浓缩物。

2. 发明申请

WO2008045852A2 IMPROVED METHODS FOR THE SEPARATION OF STREPTOCOCCUS PNEUMONIAE TYPE 3 POLYSACCHARIDES 审中-公开
标题翻译：分离杆菌型3型多糖的改进方法
公开(公告)号：WO2008045852A2
公开(公告)日：2008-04-17
申请号：PCT/US2007/080768
申请日：2007-10-09
申请人： WYETH , BAHLER, Brian, Douglas , HUGHES, Erik, Heller , LEE, Tsu-shun
发明人： BAHLER, Brian, Douglas , HUGHES, Erik, Heller , LEE, Tsu-shun
IPC分类号： C12P19/04 , A61K31/715 , A61P31/04
CPC分类号： C12P19/04 , A61K31/715 , Y10S424/831
摘要： The present invention provides improved methods for the reduction or removal of protein impurities from a complex cellular Streptococcus pneumoniae lysate or centrate comprising serotype 3 polysaccharides involving steps relating to post-lysis heating or pH adjustment. In certain methods, the lysate is heated for a time and at a temperature sufficient to denature proteins present in the lysate and cause their aggregation and precipitation. In one embodiment, the lysate is heated to at least 60°C for at least 30 minutes to cause protein aggregation and precipitation, more particularly about 60°C to about 70°C for about 30 to about 50 minutes, and even more particularly about 65°C for about 40 minutes. In other methods, the pH of the lysate or centrate is increased to at least 8.0 to improve filterability, more particularly about 8.0 to 8.4, and even more particularly about 8.2. In further methods, heating and pH adjustment steps are combined to cause the aggregation and precipitation of proteins as well as to improve filterability of the lysates or centrates. In other methods, the pH of the lysate or centrate is lowered to about 3.0 to about 5.0 to cause protein aggregation and precipitation. Such methods allow for the production of substantially purified serotype 3 polysaccharide-containing lysates or centrates.
摘要翻译：本发明提供了用于从复合细胞性肺炎链球菌裂解物或浓缩物中还原或除去蛋白质杂质的改进方法，其包含血清型3多糖，其涉及裂解加热或pH调节相关的步骤。在某些方法中，将裂解物加热一段时间并且在足以使存在于裂解物中的蛋白质变性并导致其聚集和沉淀的温度下加热。在一个实施方案中，将裂解物加热至至少60℃至少30分钟以引起蛋白质聚集和沉淀，更特别地约60℃至约70℃约30至约50分钟，甚至更特别地约为 65°C约40分钟。在其他方法中，将裂解物或浓缩物的pH增加至至少8.0以提高过滤性，更特别地约8.0至8.4，甚至更特别约8.2。在进一步的方法中，加热和pH调节步骤组合以引起蛋白质的聚集和沉淀以及改善裂解物或浓缩物的过滤性。在其他方法中，裂解物或浓缩物的pH降低至约3.0至约5.0以引起蛋白质聚集和沉淀。这样的方法允许生产基本上纯化的血清型3多糖裂解物或中心体。

3. 发明申请

WO2011139876A1 HALF CANNULA FULL CORE BIOPSY NEEDLE 审中-公开
标题翻译：半瓶颈全毛生物针头
公开(公告)号：WO2011139876A1
公开(公告)日：2011-11-10
申请号：PCT/US2011/034446
申请日：2011-04-29
申请人： COOK INCORPORATED , STANLEY, Cleon , HUGHES, Erik , TAYLOR, James , THEOBALD, Elizabeth, A. , NOWICKI, Ryan , CAVETT, Meridith
发明人： STANLEY, Cleon , HUGHES, Erik , TAYLOR, James , THEOBALD, Elizabeth, A. , NOWICKI, Ryan , CAVETT, Meridith
IPC分类号： A61B10/02
CPC分类号： A61B10/0266 , A61B2017/320064
摘要： There is disclosed, among other things, embodiments of a biopsy needle for taking a full core sample through soft tissue. In the illustrated embodiments, an inner tubular member having a semi - cylindrical distal end is within and slidable with respect to an outer tubular member. A semi - cylindrical channel is in the distal end, as well as a planar end surface. The channel has a set of one or more protrusions or texture, and a complementary set of protrusions or texture are placed on the inside of the outer member so that the sets of protrusions or texture face each other when the distal ends of the members are adjacent each other.
摘要翻译：除其他之外，公开了用于将全核样品通过软组织的活检针的实施例。在所示实施例中，具有半圆柱形远端的内部管状构件在外部管状构件内部可滑动并且可滑动。半圆柱形通道位于远端以及平面端面。通道具有一组一个或多个突起或纹理，并且在外部构件的内侧上放置一组互补的突起或纹理，使得当构件的远端相邻时，这些突起或纹理组彼此面对彼此。

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