专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2012128580A1 WATER-SOLUBLE POLYPEPTIDES COMPRISED OF REPEAT MODULES, METHOD FOR PREPARING THE SAME AND METHOD FOR A TARGET-SPECIFIC POLYPEPTIDE AND ANALYSIS OF BIOLOGICAL ACTIVITY THEREOF 审中-公开
标题翻译：重复模块包含的水溶性聚合物，其制备方法和目标特异性多肽的方法及其生物活性分析
公开(公告)号：WO2012128580A1
公开(公告)日：2012-09-27
申请号：PCT/KR2012/002094
申请日：2012-03-22
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , KIM, Hak Sung , KIM, Dong Sup , LEE, Sang Chul , LEE, Byung Chul , HAN, Ji Eun , LEE, Joong Jae , PARK, Keun Wan , HONG, Seung Pyo
发明人： KIM, Hak Sung , KIM, Dong Sup , LEE, Sang Chul , LEE, Byung Chul , HAN, Ji Eun , LEE, Joong Jae , PARK, Keun Wan , HONG, Seung Pyo
IPC分类号： C07K19/00 , C12N15/62
CPC分类号： C07K14/195 , C07K14/46 , C07K14/461 , C07K14/705 , C07K2319/35 , C07K2319/70 , C12N15/70 , C12Q1/6897 , G01N33/5023 , G06F19/16
摘要： The present invention relates to a soluble polypeptide comprised of repeat modules. More particularly, the present invention relates to a soluble fusion polypeptide of the N-terminal domain of internalin and LRR (Leucine rich repeat) family protein, a method for preparing the polypeptide, a vector comprising a nucleic acid sequence encoding the polypeptide, a host cell comprising the vector, a method for producing a solubility and folding-improved fusion polypeptide by expressing the vector in the host cell, and a method for improving the solubility and folding of the fusion polypeptide. Further, the present invention relates to a method for preparing the polypeptide bound with a specific target and analyzing the efficacy of the soluble polypeptide.
摘要翻译：本发明涉及由重复模块组成的可溶性多肽。更具体地说，本发明涉及内源蛋白和LRR（富含亮氨酸重复）家族蛋白质的N-末端结构域的可溶性融合多肽，制备该多肽的方法，包含编码该多肽的核酸序列的载体，宿主包含载体的细胞，通过在宿主细胞中表达载体产生溶解性和折叠改良的融合多肽的方法，以及改善融合多肽的溶解性和折叠的方法。此外，本发明涉及制备与特异性靶标结合的多肽的方法，并分析可溶性多肽的功效。

2. 发明申请

WO2012091812A1 USE OF SACCHAROMYCES CEREVISIAE SUC2 GENE IN YARROWIA LIPOLYTICA FOR SUCROSE UTILIZATION 审中-公开
标题翻译：在沙鼠中使用沙门氏菌SUC2基因进行枯草杆菌利用
公开(公告)号：WO2012091812A1
公开(公告)日：2012-07-05
申请号：PCT/US2011/060788
申请日：2011-11-15
申请人： E. I. du Pont de Nemours and Company , HONG, Seung-Pyo , SEIP, John E. , ZHU, Quinn, Qun
发明人： HONG, Seung-Pyo , SEIP, John E. , ZHU, Quinn, Qun
IPC分类号： C12N1/19 , C12N9/26 , C12N9/60 , C12P7/64 , C12N15/62
CPC分类号： C12N9/2431 , C07K2319/02 , C12N9/60 , C12P7/64 , C12Y302/01026
摘要： Disclosed herein are transformed Yarrowia lipolytica comprising an exogenous polynucleotide encoding a polypeptide having sucrose invertase activity. Also disclosed are methods of using the transformed Y. lipolytica.
摘要翻译：本文公开的是转化的解脂耶氏酵母病，其包含编码具有蔗糖转化酶活性的多肽的外源多核苷酸。还公开了使用转化的解脂耶氏酵母的方法。

3. 发明申请

WO2012027689A1 RECOMBINANT MICROBIAL HOST CELLS FOR HIGH EICOSAPENTAENOIC ACID PRODUCTION 审中-公开
标题翻译：用于高效酸生产的重组微生物宿主细胞
公开(公告)号：WO2012027689A1
公开(公告)日：2012-03-01
申请号：PCT/US2011/049384
申请日：2011-08-26
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , HONG, Seung-Pyo , SHARPE, Pamela, L. , XUE, Zhixiong , YADAV, Narendra, S. , ZHANG, Hongxiang , ZHU, Qun
发明人： HONG, Seung-Pyo , SHARPE, Pamela, L. , XUE, Zhixiong , YADAV, Narendra, S. , ZHANG, Hongxiang , ZHU, Qun
IPC分类号： C12N15/54 , C12P7/64
CPC分类号： C12N9/1029 , C12N9/0071 , C12N15/815 , C12P7/6472
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica are disclosed herein that are capable of producing microbial oil comprising greater than 25 weight percent of eicosapentaenoic acid ["EPA"], an omega-3 polyunsaturated fatty acid, measured as a weight percent of dry cell weight.
摘要翻译：本文公开了含油酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，其能够产生包含大于25重量％的二十碳五烯酸[“EPA”]的微生物油，其为以干细胞重量的重量百分比测量的ω-3多不饱和脂肪酸。

4. 发明申请

WO2010147907A1 HIGH EICOSAPENTAENOIC ACID OILS FROM IMPROVED OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA 审中-公开
标题翻译：来自改良的YARROWIA LIPOLYTICA优化菌株的高选择性酸性油
公开(公告)号：WO2010147907A1
公开(公告)日：2010-12-23
申请号：PCT/US2010/038539
申请日：2010-06-14
申请人： E. I. du Pont de Nemours and Company , HONG, Seung-Pyo , SHARPE, Pamela, L. , XUE, Zhixiong , YADAV, Narendra, S. , ZHU, Qun
发明人： HONG, Seung-Pyo , SHARPE, Pamela, L. , XUE, Zhixiong , YADAV, Narendra, S. , ZHU, Qun
IPC分类号： A23D9/00 , A23K1/00 , A23K1/16 , A23K1/18 , A23L1/03 , A23L1/29 , A23L1/30 , C12N15/01 , C12N15/81 , C12P7/64
CPC分类号： A21D2/165 , A23K10/12 , A23K20/158 , A23K50/80 , A23L33/12 , A23L33/40 , A23V2002/00 , C11B1/10 , C12N9/0083 , C12N9/1029 , C12N9/93 , C12N15/815 , C12P7/6427 , C12P7/6472 , C12P7/6481 , A23V2250/187 , A23V2250/1872 , A23V2250/19
摘要： Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid ["EPA"], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids ["% TFAs"] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C 16/18 elongases, and over-express malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The strains possess at least one peroxisome biogenesis factor protein knockout. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.
摘要翻译：描述了能够产生包含大于50重量％的二十碳五烯酸[“EPA”]的油的油性酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，以总脂肪酸的重量百分比测量的α-3多不饱和脂肪酸[“％ TFAs“]，并且具有至少3.1％的EPA％TFA与比率为TFA的亚油酸的比率。这些菌株除了其它异源β9延伸酶，α8去饱和酶，α5去饱和酶，β17去饱和酶，β12去饱和酶，C16 / 18延伸酶和过量表达以外，还过表达至少一种Δ9延伸酶/α8去饱和酶多酶，表达丙二酰辅酶A合成酶和/或酰基辅酶A溶血磷脂酰基转移酶。该菌株具有至少一种过氧化物酶体生物发生因子蛋白质敲除。要求在所述宿主细胞内生产EPA的方法，从细胞获得的油及其产物。

5. 发明申请

WO2008032911A1 CELL SURFACE EXPRESSION VECTOR OF MYOSYATIN AND MICROORGANISMS TRANSFORMED THEREWITH 审中-公开
标题翻译：肌醇蛋白的细胞表面表达载体和转化的微生物
公开(公告)号：WO2008032911A1
公开(公告)日：2008-03-20
申请号：PCT/KR2007/000856
申请日：2007-02-16
申请人： BIOLEADERS CORPORATION , KIM, Chul Joong , SUNG, Moon-Hee , HONG, Seung-Pyo , LEE, Jong-Soo , LEE, Il Han , XU, Long Chun , KIM, Jong-Taik , CHOI, Yoon-Ho
发明人： KIM, Chul Joong , SUNG, Moon-Hee , HONG, Seung-Pyo , LEE, Jong-Soo , LEE, Il Han , XU, Long Chun , KIM, Jong-Taik , CHOI, Yoon-Ho
IPC分类号： C12N15/63
CPC分类号： C12N15/74 , A61K38/00 , A61K39/00 , C07K14/475 , C12N9/93
摘要： The present invention relates to a vector expressing myostatin mature proteins, a recombinant microorganism transformed with the vector, and a feedstuff additive or a vaccine containing the myostatin mature proteins expressed on the cell surface thereof, and more particularly, to a cell surface expression vector containing a polynucleotide encoding a myostatin mature protein and a gene encoding a poly-gamma-glutamate synthetase complex which induces surface expression in a microorganism, a microorganism transformed with the vector, and a feedstuff additive or a vaccine, containing the myostatin mature proteins prepared by culturing the transformed microorganism, as an effective ingredient. The inventive feedstuff additive or vaccine containing the myostatin mature proteins expressed on a cell surface, as an effective ingredient, can be used for preventing and treating muscle-wasting diseases and degenerative diseases such as muscular dystrophy, muscular atrophy and the like, as well as increasing and controlling muscle growth of livestock and poultry. In addition, the transformed strain onserved in the knockout mice, shows the same effect even if the strain itself after culturing is directly used, and thus it is very economical.
摘要翻译：本发明涉及表达肌生成抑制素成熟蛋白的载体，用载体转化的重组微生物，以及含有在细胞表面上表达的肌生成抑制素成熟蛋白质的饲料添加剂或疫苗，更具体地说，涉及含有编码肌生成抑制素成熟蛋白的多核苷酸和编码在微生物中表达表达的多聚谷氨酸合成酶复合物的基因，用载体转化的微生物，以及含有通过培养制备的肌生成抑制素成熟蛋白的饲料添加剂或疫苗转化的微生物作为有效成分。作为有效成分，含有在细胞表面表达的肌生成抑制素成熟蛋白质的本发明的饲料添加剂或疫苗可用于预防和治疗肌肉消瘦疾病和退行性疾病如肌营养不良症，肌肉萎缩症等，以及增加和控制家畜和家禽的肌肉生长。此外，即使直接使用培养后的菌株本身，所以存在于敲除小鼠中的转化菌株也显示相同的效果，因此是非常经济的。

6. 发明申请

WO2008005418A2 ASSAYS FOR TAK1 ACTIVITY 审中-公开
标题翻译： TAK1活动测定
公开(公告)号：WO2008005418A2
公开(公告)日：2008-01-10
申请号：PCT/US2007015330
申请日：2007-06-29
申请人： UNIV COLUMBIA , MOMCILOVIC MILLICA , CARLSON MARIAN , HONG SEUNG-PYO
发明人： MOMCILOVIC MILLICA , CARLSON MARIAN , HONG SEUNG-PYO
CPC分类号： G01N33/5041 , C12Q1/025 , G01N2333/912 , G01N2500/10
摘要： This invention discloses methods to identify modulators of TAK1 activity comprising measuring TAK1 activity or measuring TAK1- dependent Snf1/AMPK activity. Further disclosed are methods to measure TAK1 activity comprising measuring phosphorylation of an AMPK substrate or an increase in AMPK activity resulting from an increase in TAK1 activity. Methods to treat obesity, diabetes and diseases characterized by inflammation by administering compounds that modulate TAK1 are described also.
摘要翻译：本发明公开了鉴定TAK1活性调节剂的方法，包括测量TAK1活性或测量TAK1依赖性Snf1 / AMPK活性。还公开了测量TAK1活性的方法，包括测量AMPK底物的磷酸化或由TAK1活性增加导致的AMPK活性的增加。还描述了通过施用调节TAK1的化合物治疗肥胖症，糖尿病和炎症特征的疾病的方法。

7. 发明申请

WO2005075653A1 CELL SURFACE EXPRESSION VECTOR OF PARVOVIRUS ANTIGEN AND MICROORGANISMS TRANSFORMED THEREOF 审中-公开
标题翻译： PARVOVIRUS抗原的细胞表面表达载体及其转化的微生物
公开(公告)号：WO2005075653A1
公开(公告)日：2005-08-18
申请号：PCT/KR2005/000356
申请日：2005-02-04
申请人： BIOLEADERS CORPORATION , M.D. LAB Co. Ltd , BIOLEADERS JAPAN CORPORATION , IMBIO CORPORATION , SUNG, Moon Hee , KIM, Chul Joong , JUNG, Chang Min , HONG, Seung Pyo , LEE, Jong Su , CHOI, Jae Chul , SEWAKI, Motomitsu , KIM, Jong-Taik
发明人： SUNG, Moon Hee , KIM, Chul Joong , JUNG, Chang Min , HONG, Seung Pyo , LEE, Jong Su , CHOI, Jae Chul , SEWAKI, Motomitsu , KIM, Jong-Taik
IPC分类号： C12N15/63
CPC分类号： C07K14/005 , C12N15/74 , C12N2750/14322
摘要： The present invention relates to a surface expression vector expressing a parvovirus antigen on the surface of microorganisms, the vector containing not only a gene encoding the capsid antigen protein of parvovirus causing canine parvovirus (CPV) infection and feline panleukopenia (FLP) but also at least one of pgsB, pgsC and pgsA genes encoding a poly-gamma-glutamate synthetase complex. Also, the present invention relates to microorganisms transformed with the surface expression vector, and parvovirus vaccines containing the transformed microorganisms. According to the present invention, the use of the recombinant bacterial strains expressing the parvovirus antigen on their surface allows the economical production of vaccines for the treatment and prevention of canine parvovirus infection and feline panleukopenia.
摘要翻译：本发明涉及表达微生物表面的细小病毒抗原的表达表达载体，该载体不仅含有编码引起犬细小病毒（CPV）感染和猫泛白细胞减少症（FLP）的细小病毒的衣壳抗原蛋白的基因，而且至少编码聚-γ-谷氨酸合成酶复合物的pgsB，pgsC和pgsA基因之一。此外，本发明涉及用表面表达载体转化的微生物和含有转化的微生物的细小病毒疫苗。根据本发明，在其表面上使用表达细小病毒抗原的重组细菌菌株可以经济地生产用于治疗和预防犬细小病毒感染和猫泛白细胞减少症的疫苗。

8. 发明申请

WO2004108937A1 CELL SURFACE EXPRESSION VECTOR OF SARS VIRUS ANTIGEN AND MICROORGANISMS TRANSFORMED THEREBY 审中-公开
标题翻译： SARS病毒抗原和微生物转化的细胞表面表达载体
公开(公告)号：WO2004108937A1
公开(公告)日：2004-12-16
申请号：PCT/KR2004/001341
申请日：2004-06-04
申请人： BIOLEADERS CORPORATION , M.D. LAB , BIOLEADERS JAPAN CORP. , KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , SUNG, Moon Hee , KIM, Chul Joong , JUNG, Chang Min , HONG, Seung Pyo , LEE, Jong Su , CHOI, Jae Chul , KIM, Kwang , SHUNICHI, Kuroda , POO, Ha Ryoung
发明人： SUNG, Moon Hee , KIM, Chul Joong , JUNG, Chang Min , HONG, Seung Pyo , LEE, Jong Su , CHOI, Jae Chul , KIM, Kwang , SHUNICHI, Kuroda , POO, Ha Ryoung
IPC分类号： C12N15/86
CPC分类号： C07K14/005 , A61K39/00 , A61K2039/523 , C07K2319/00 , C12N9/93 , C12N15/74 , C12N2710/20022 , C12N2770/20022 , Y02A50/472 , Y02A50/476
摘要： The present invention relates to a surface expression vector of SARS coronavirus antigen containing a gene encoding an antigen of SARS inducing coronavirus and any one or two or more of genes pgsB, pgsC and pgsA encoding poly-gamma-glutamic acid synthase complex, a microorganism transformed by the surface expression vector, and a SARS vaccine comprising the microorganism. According to the present invention, it is possible to economically produce a vaccine for prevention and treatment of SARS using a recombinant strain expressing an SARS coronavirus antigen on their surface.
摘要翻译：本发明涉及SARS冠状病毒抗原的表达表达载体，其含有编码SARS诱导冠状病毒抗原的基因和编码聚-γ-谷氨酸合酶复合物的pgsB，pgsC和pgsA基因中的一种或两种以上的基因，微生物转化通过表面表达载体和包含该微生物的SARS疫苗。根据本发明，可以在表面上使用表达SARS冠状病毒抗原的重组菌株经济地制备用于预防和治疗SARS的疫苗。

9. 发明申请

WO2004007593A1 POLY-GAMMA-GLUTAMATE HAVING ULTRA HIGH MOLECULAR WEIGHT AND METHOD FOR USING THE SAME 审中-公开
标题翻译：具有超高分子量的聚氨基葡糖酸酯及其使用方法
公开(公告)号：WO2004007593A1
公开(公告)日：2004-01-22
申请号：PCT/KR2003/001369
申请日：2003-07-10
申请人： BIOLEADERS CORP , SUNG, Moon-Hee , PARK, Chung , HONG, Seung-Pyo , KIM, Kwang Seok , SONG, Jae Jun , RHA, Eu Gene , KIM, Kwang , SODA, Kenji , ASHIUCHI, Makoto
发明人： SUNG, Moon-Hee , PARK, Chung , HONG, Seung-Pyo , KIM, Kwang Seok , SONG, Jae Jun , RHA, Eu Gene , KIM, Kwang , SODA, Kenji , ASHIUCHI, Makoto
IPC分类号： C08H1/00
CPC分类号： A61K9/0014 , A61K8/88 , A61K9/0095 , A61K47/42 , A61Q19/00 , C08H1/00 , C12P21/02
摘要： The present invention relates to a poly-gamma-glutamate (PGA) having an ultra-high molecular weight greater than 5,000 kDa. The ultra-high molecular weight PGA according to the present invention has a mean molecular weight greater than 13,000 kDa, and more than 95 % of its molecules have a molecular weight ranging from 3,000 to 15,000 kDa. Also, it can be produced by the culturing of Bacillus subtilis var. chungkookjang . The ultra-high molecular weight PGA according to the present invention shows very excellent moisture-absorbing, moisture-retaining, sustained release, mineral solubility, and water-absorbing properties, and thus, can be used as a new and high value-added material in various applications.
摘要翻译：本发明涉及超高分子量大于5,000kDa的聚-γ-谷氨酸（PGA）。根据本发明的超高分子量PGA的平均分子量大于13000kDa，其分子的95％以上具有3,000至15,000kDa的分子量。此外，它可以通过培养枯草芽孢杆菌变种产生。 chungkookjang。根据本发明的超高分子量PGA显示出非常优异的吸湿性，保湿性，缓释性，矿物溶解性和吸水性，因此可用作新的高附加值材料在各种应用中。

10. 发明申请

WO2012162368A1 EXPRESSION OF CALEOSIN IN RECOMBINANT OLEAGINOUS MICROORGANISMS TO INCREASE OIL CONTENT THEREIN 审中-公开
标题翻译：重组蛋白微生物中的高糖表达增加其内含物
公开(公告)号：WO2012162368A1
公开(公告)日：2012-11-29
申请号：PCT/US2012/039100
申请日：2012-05-23
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , HONG, Seung-Pyo , ZHU, Quinn Qun
发明人： HONG, Seung-Pyo , ZHU, Quinn Qun
IPC分类号： C07K14/37 , C07K14/415 , C12P7/64
CPC分类号： C07K14/37 , C12P7/6463 , C12P7/6472
摘要： Recombinant oleaginous microorganisms having increased oil content due to the expression of a caleosin polypeptide are described. A recombinant oleaginous microorganism of the disclosed invention produces at least 25% of its dry cell weight as oil, and comprises a functional polyunsaturated fatty acid (PUFA) biosynthetic pathway and at least one genetic construct encoding a caleosin polypeptide. A method for increasing the amount of oil in a recombinant oleaginous microorganism is also described.
摘要翻译：描述了由于表达Caleosin多肽而具有增加的油含量的重组含油微生物。本发明公开的本发明的重组含油微生物作为油产生至少25％的干细胞重量，并且包含功能性多不饱和脂肪酸（PUFA）生物合成途径和至少一种编码碳水化合物多肽的遗传构建体。还描述了用于增加重组含油微生物中的油量的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式