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1. 发明申请

WO2005064012A3 METHOD FOR VALIDATING AND/OR CALIBRATING A SYSTEM FOR PERFORMING HYBRIDISATION EXPERIMENTS, MICROARRAY, AND KIT THEREFOR 审中-公开
标题翻译：方法验证和/或校准SYSTEM FOR杂交实验的实施，MICRO ARRAY及其试剂盒
公开(公告)号：WO2005064012A3
公开(公告)日：2005-09-09
申请号：PCT/EP2004014414
申请日：2004-12-17
申请人： ALOPEX GMBH , MANN WOLFGANG , KRISPIN OLIVER , HOFFMUELLER PETRA
发明人： MANN WOLFGANG , KRISPIN OLIVER , HOFFMUELLER PETRA
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01J2219/00693 , C12Q2545/107 , C12Q2527/107
摘要： The invention relates to a method for calibrating and/or validating a system for performing a microarray experiment, in addition to a microarray, and a kit therefor. The inventive method comprises the following steps: a) a microarray is prepared, said microarray comprising at least one first measuring point containing probe molecules (PM) that are complementary to the target molecules and can form, with the target molecules, hybrids that have a melting temperature Tm(PM), and at least one second measuring point containing probe molecules (MM1) that are partially complementary to the target molecules and can form, with the target molecules, partial hybrids that have a melting temperature Tm(MM1); b) target molecules are provided, that are complementary to probe molecules (PM) in at least one first measuring point of the microarray; c) a hybridisation experiment is performed with the microarray and the target molecules, with variation of a defined parameter; and d) the signal intensities are determined according to the variation of the parameter. The parameter value at the maximum difference between the signal intensities of the first and second measuring points is the parameter value for which the system is approximately in equilibrium in relation to the hybrids in the first measuring point, and according to which the system is calibrated.
摘要翻译：本发明是用于校准和/或验证用于进行微阵列实验的系统的方法以及用于其的微阵列和试剂盒。具有至少一个第一测量点在其中探针分子（PM），其互补于目标分子和可以从形式与具有的熔融温度Tm（PM）的目标分子杂交来选择 - a）提供的微阵列，包括：根据本发明的方法包括以下步骤， - 至少一个第二测量点在其中探针分子（MM1），它们不完全互补于靶分子和其可形成与目标分子，其具有的熔融温度Tm（MM1）不正确杂交其中b）以提供目标分子（探测分子PM）是互补的，以与微阵列进行杂交实验的微阵列的一个或MEH-reren第一测量点，以及c）以及在一定的参数的变化的目标分子，d）确定的信号强度作为参数的变化的函数，其中，在所述参数值是之间的最大差值SIG-nalintensitäten参数值处相对于所述第一测量点的杂交系统是大致平衡，并且该系统被校准的第一和第二测量点。

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