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    • 7. 发明申请
    • SOLID-PHASE OLIGOSACCHARIDE TAGGING: A TECHNIQUE FOR MANIPULATION OF IMMOBILIZED CARBOHYDRATES
    • 固相低密度脂蛋白标签:用于操纵固定化碳酸氢盐的技术
    • WO2006084461A1
    • 2006-08-17
    • PCT/DK2006/000066
    • 2006-02-08
    • CARLSBERG A/SLOHSE, AndersJØRGENSEN, Malene RyborgMARTINS, RitaHINDSGAUL, Ole
    • LOHSE, AndersJØRGENSEN, Malene RyborgMARTINS, RitaHINDSGAUL, Ole
    • G01N33/543
    • G01N33/6842G01N33/54353G01N33/6803Y10T436/145555Y10T436/24
    • The invention relates to methods of manipulating immobilised carbohydrates by de-rivatisation. Depending on the nature of the derivatisation, the carbohydrate may thereby be more easily detected and/or identified or handled. In particular, the inven-tion relates to methods of preparing a reactive sugar comprising the steps of: i) pro-viding a sample comprising a reducing sugar; ii) providing a solid support covalently attached to a linker comprising a capture group comprising an -NH2 group, wherein said linker optionally is attached to said solid support via a spacer; iii) reacting said reducing sugar with said -NH2 group, thereby obtaining an immobilised sugar; iv) reacting free -NH2 groups with a capping agent, wherein the capping agent com-prises a reactive group capable of reacting with an -NH2 group; and v) reducing C=N bonds with a reducing agent, thereby obtaining an reactive sugar of the struc-ture SugarCHn-NH- linked to a solid support via a linker and optionally a spacer, wherein n is 1 or 2.
    • 本发明涉及通过脱核化来操作固定的碳水化合物的方法。 根据衍生化的性质,可以更容易地检测和/或识别或处理碳水化合物。 特别地,本发明涉及制备反应性糖的方法,包括以下步骤:i)预处理包含还原糖的样品; ii)提供共价连接到包含-NH 2基团的捕获基团的接头的固体支持物,其中所述接头任选地经由间隔子连接到所述固体支持物上; iii)使所述还原糖与所述-NH 2基团反应,从而获得固定的糖; iv)使游离-NH 2基团与封端剂反应,其中封端剂包含能够与-NH 2基团反应的反应性基团; 和v)用还原剂还原C = N键,从而通过接头和任选的间隔基获得与固体支持物连接的结构糖基-NH-的反应性糖,其中n为1或2。