专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

IL251945D0 Method for protein display on teh surface of cells 未知
公开(公告)号：IL251945D0
公开(公告)日：2017-06-29
申请号：IL25194517
申请日：2017-04-26
申请人： MERCK PATENT GMBH , GUENTHER RALF , BECKER STEFAN , RHIEL LAURA , HOCK BJOERN , HELMAN DANIEL , TOISTER ACHITUV MIRA
发明人： GUENTHER RALF , BECKER STEFAN , RHIEL LAURA , HOCK BJOERN , HELMAN DANIEL , TOISTER-ACHITUV MIRA
IPC分类号： C12N20060101
摘要： The present invention provides methods for the non-covalent surface display of proteins of interest (POI), in particular for Fc-domain containing proteins such as antibodies. The inventive method may be used to screen and select proteins of interest of a desired phenotype. The present invention further discloses polynucleotides and proteins and methods of producing the same, which may be used in carrying out the inventive method.

2. 发明专利

IL211583D0 LOW FUCOSE CELL LINES AND USES THEREOF 未知
公开(公告)号：IL211583D0
公开(公告)日：2011-06-30
申请号：IL21158311
申请日：2011-03-06
申请人： MERCK SERONO SA , BAR SHIMON MEIRAV , TOISTER ACHITUV MIRA , HELMAN DANIEL
IPC分类号： C12P20060101

3. 发明申请

WO2012085911A1 MEMBRANE BOUND REPORTER MOLECULES AND THEIR USE IN CELL SORTING 审中-公开
标题翻译：膜结合报告分子及其在细胞分选中的应用
公开(公告)号：WO2012085911A1
公开(公告)日：2012-06-28
申请号：PCT/IL2011/000956
申请日：2011-12-19
申请人： MERCK SERONO S.A , HELMAN, Daniel , TOISTER-ACHITUV, Mira , BAR-SHIMON, Meirav , SMOLARSKY, Moshe
发明人： HELMAN, Daniel , TOISTER-ACHITUV, Mira , BAR-SHIMON, Meirav , SMOLARSKY, Moshe
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： G01N33/56966 , C07K2319/035 , C07K2319/20 , C12N15/85 , C12N2830/20 , C12N2830/40 , C12Q1/6897 , C12Q2563/131
摘要： The present invention relates to nucleic acid molecules comprising a nucleic acid sequence encoding a membrane-bound biotin mimetic peptide (BMP) or biotin acceptor peptide (BAP). The invention also relates to a method for selection of high producer cells secreting a protein of interest.
摘要翻译：本发明涉及包含编码膜结合生物素模拟肽（BMP）或生物素受体肽（BAP）的核酸序列的核酸分子。本发明还涉及用于选择分泌目的蛋白质的高生产细胞的方法。

4. 发明申请

WO2012120500A3 LOW FUCOSE CELL LINES AND USES THEREOF 审中-公开
标题翻译：低韧性细胞系及其用途
公开(公告)号：WO2012120500A3
公开(公告)日：2012-11-15
申请号：PCT/IL2012000109
申请日：2012-03-05
申请人： MERCK SERONO SA , HELMAN DANIEL , BAR-SHIMON MEIRAV , TOISTER-ACHITUV MIRA
发明人： HELMAN DANIEL , BAR-SHIMON MEIRAV , TOISTER-ACHITUV MIRA
IPC分类号： C12N15/10 , C12N9/10 , C12N15/01
CPC分类号： C07K16/2863 , C07K16/00 , C07K2317/41 , C07K2319/30 , C12N9/0006 , C12N9/1051 , C12N9/88 , C12N15/01 , C12N15/85 , C12Y101/01271 , C12Y204/01068 , C12Y402/01047
摘要： A method of selecting cells having zero fucose level useful as host cells for expressing recombinant proteins is disclosed. The method comprises: (d) introducing genetic mutations into a population of CHO cells by contacting the cells with a methotrexate (MTX), (e) contacting the population of CHO cells comprising mutated cells with a non-toxic fucose binding agent for an amount of time that allows binding of the fucose binding agent to a fucose moiety on a cell membrane of the population of cells, wherein the amount of time does not allow killing of the cells; and (f) depleting from the population of cells comprising mutated cells, a subpopulation of cells which bind the fucose binding agent, thereby selecting cells useful as host cells for expressing recombinant proteins, the selected cells having zero fucose content. There are also disclosed cells and cell lines useful as host cells for expressing recombinant proteins.
摘要翻译：公开了一种选择具有零岩藻糖水平的细胞用作表达重组蛋白的宿主细胞的方法。该方法包括：（d）通过使细胞与甲氨蝶呤（MTX）接触将基因突变引入CHO细胞群体中，（e）使包含突变细胞的CHO细胞群体与无毒岩藻糖结合剂接触一定量允许岩藻糖结合剂与细胞群的细胞膜上的岩藻糖部分结合的时间，其中时间量不允许细胞被杀死; 和（f）从包含突变细胞的细胞群中消耗结合岩藻糖结合剂的细胞亚群，由此选择可用作表达重组蛋白质的宿主细胞的细胞，所选择的细胞具有零岩藻糖含量。还公开了用作表达重组蛋白的宿主细胞的细胞和细胞系。

5. 发明申请

WO2012120500A2 LOW FUCOSE CELL LINES AND USES THEREOF 审中-公开
标题翻译：低FUCOSE细胞系及其用途
公开(公告)号：WO2012120500A2
公开(公告)日：2012-09-13
申请号：PCT/IL2012/000109
申请日：2012-03-05
申请人： MERCK SERONO S.A. , HELMAN, Daniel , BAR-SHIMON, Meirav , TOISTER-ACHITUV, Mira
发明人： HELMAN, Daniel , TOISTER-ACHITUV, Mira
IPC分类号： C12N15/10
CPC分类号： C07K16/2863 , C07K16/00 , C07K2317/41 , C07K2319/30 , C12N9/0006 , C12N9/1051 , C12N9/88 , C12N15/01 , C12N15/85 , C12Y101/01271 , C12Y204/01068 , C12Y402/01047
摘要： A method of selecting cells having zero fucose level useful as host cells for expressing recombinant proteins is disclosed. The method comprises: (d) introducing genetic mutations into a population of CHO cells by contacting the cells with a methotrexate (MTX), (e) contacting the population of CHO cells comprising mutated cells with a non-toxic fucose binding agent for an amount of time that allows binding of the fucose binding agent to a fucose moiety on a cell membrane of the population of cells, wherein the amount of time does not allow killing of the cells; and (f) depleting from the population of cells comprising mutated cells, a subpopulation of cells which bind the fucose binding agent, thereby selecting cells useful as host cells for expressing recombinant proteins, the selected cells having zero fucose content. There are also disclosed cells and cell lines useful as host cells for expressing recombinant proteins.
摘要翻译：公开了选择具有用作表达重组蛋白的宿主细胞的零岩藻糖水平的细胞的方法。该方法包括：（d）通过使细胞与甲氨蝶呤（MTX）接触，将遗传突变引入CHO细胞群体;（e）使包含突变细胞的CHO细胞群与无毒岩藻糖结合剂的量接触一定量的时间，其允许岩藻糖结合剂与细胞群的细胞膜上的岩藻糖部分结合，其中所述时间量不允许杀死细胞; 和（f）从包含突变细胞的细胞群体中消耗，细胞结合岩藻糖结合剂的细胞亚群，从而选择用作表达重组蛋白质的宿主细胞的细胞，所选择的细胞具有零岩藻糖含量。还公开了可用作表达重组蛋白的宿主细胞的细胞和细胞系。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式