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1. 发明申请

WO2010054362A3 TEMPERATURE-INDUCED POLYNUCLEOTIDES AND USES THEREFOR 审中-公开
公开(公告)号：WO2010054362A3
公开(公告)日：2010-05-14
申请号：PCT/US2009/063838
申请日：2009-11-10
申请人： WYETH LLC , MCCARTHY, Kevin, M. , HELLER-HARRISON, Robin , LEONARD, Mark, W. , CHARLEBOIS, Timothy, S.
发明人： MCCARTHY, Kevin, M. , HELLER-HARRISON, Robin , LEONARD, Mark, W. , CHARLEBOIS, Timothy, S.
IPC分类号： C07K14/47 , C12N15/67 , C12N15/85
摘要： The present invention is based on the discovery that the transcriptional activity of both hamster mammary tumor 7 (HMT 7) and hamster layilin genes are increased when Chinese Hamster Ovary (CHO) cells are cultured at temperatures lower than 37°C. The present invention provides novel genomic, cDNA, and protein sequences of HMT 7 and a novel genomic sequence for hamster layilin. Included in the genomic sequences are novel temperature-induced promoter sequences for each; two promoter sequences are provided for HMT 7 and one promoter sequence is provided for layilin. The invention additionally provides antisense and siRNA molecules to the nucleic acid molecules of HMT 7. The invention also provides genetically engineered expression vectors comprising the novel temperature-induced promoters of the invention, which are particularly useful as part of a mammalian temperature-inducible expression system. The invention also provides host cells and/or transgenic animals comprising the novel nucleic acid molecules of the invention. The invention further provides methods of using the polynucleotides of the invention, e.g., for temperature-induced transgene expression.

2. 发明申请

WO2007056062A3 METHODS FOR ADAPTING MAMMALIAN CELLS 审中-公开
标题翻译：适应MAMMALIAN细胞的方法
公开(公告)号：WO2007056062A3
公开(公告)日：2007-08-16
申请号：PCT/US2006042815
申请日：2006-11-02
申请人： WYETH CORP , LEE GENE W , RICHARDS D TROY , KOPYCINSKI KATHLEEN , FECKO JILL K , CHARLEBOIS TIMOTHY S , MELVILLE MARK , HELLER-HARRISON ROBIN A , SINACORE MARTIN S , LEONARD MARK
发明人： LEE GENE W , RICHARDS D TROY , KOPYCINSKI KATHLEEN , FECKO JILL K , CHARLEBOIS TIMOTHY S , MELVILLE MARK , HELLER-HARRISON ROBIN A , SINACORE MARTIN S , LEONARD MARK
IPC分类号： C12N5/00
CPC分类号： C12N5/0018 , C12N2510/02
摘要： Methods of adapting cells, e.g., mammalian cells, to a cell culture process are provided. When the adapted cells are genetically modified and used for protein production, they exhibit beneficial characteristics, such as being able to attain higher cell densities and/or achieve a higher overall yield of the produced protein.
摘要翻译：提供使细胞例如哺乳动物细胞适应细胞培养过程的方法。当适应的细胞被遗传修饰并用于蛋白质生产时，它们表现出有益的特征，例如能够获得更高的细胞密度和/或获得更高的产生的蛋白质的总产率。

3. 发明申请

WO2009114693A1 METHOD FOR IDENTIFYING CELLS SUITABLE FOR LARGE-SCALE PRODUCTION OF RECOMBINANT PROTEINS 审中-公开
标题翻译：鉴定适用于大规模生产重组蛋白的细胞的方法
公开(公告)号：WO2009114693A1
公开(公告)日：2009-09-17
申请号：PCT/US2009/036962
申请日：2009-03-12
申请人： WYETH , MCCARTHY, Kevin , HONE, Megan , HELLER-HARRISON, Robin , LEONARD, Mark
发明人： MCCARTHY, Kevin , HONE, Megan , HELLER-HARRISON, Robin , LEONARD, Mark
IPC分类号： C12P21/00 , C12N15/85 , C12N15/10 , C12N15/90
CPC分类号： C12N15/85 , C07K16/00 , C07K16/2803 , C12N2830/42 , C12N2840/203
摘要： The present invention provides methods of identifying a clonal population of cells suitable for large-scale production of a protein of interest. The invention further provides methods for high-throughput screening for genetic rearrangements in the gene encoding the protein of interest, whereby the absence of a deletion in the gene encoding the protein of interest indicates that the cell is suitable for large-scale production of the protein of interest.
摘要翻译：本发明提供了鉴定适合于大规模生产目的蛋白质的细胞的克隆群体的方法。本发明还提供了用于在编码目的蛋白质的基因中进行遗传重排的高通量筛选的方法，由此在编码目的蛋白质的基因中缺失缺失表明该细胞适合于大规模生产蛋白质出于兴趣。

4. 发明申请

WO2010054362A2 TEMPERATURE-INDUCED POLYNUCLEOTIDES AND USES THEREFOR 审中-公开
标题翻译：温度诱导的多核苷酸及其用途
公开(公告)号：WO2010054362A2
公开(公告)日：2010-05-14
申请号：PCT/US2009063838
申请日：2009-11-10
申请人： WYETH LLC , MCCARTHY KEVIN M , HELLER-HARRISON ROBIN , LEONARD MARK W , CHARLEBOIS TIMOTHY S
发明人： MCCARTHY KEVIN M , HELLER-HARRISON ROBIN , LEONARD MARK W , CHARLEBOIS TIMOTHY S
IPC分类号： C07K14/47 , C12N15/67 , C12N15/85
CPC分类号： C07K14/47 , C12N15/63
摘要： The present invention is based on the discovery that the transcriptional activity of both hamster mammary tumor 7 (HMT 7) and hamster layilin genes are increased when Chinese Hamster Ovary (CHO) cells are cultured at temperatures lower than 37°C. The present invention provides novel genomic, cDNA, and protein sequences of HMT 7 and a novel genomic sequence for hamster layilin. Included in the genomic sequences are novel temperature-induced promoter sequences for each; two promoter sequences are provided for HMT 7 and one promoter sequence is provided for layilin. The invention additionally provides antisense and siRNA molecules to the nucleic acid molecules of HMT 7. The invention also provides genetically engineered expression vectors comprising the novel temperature-induced promoters of the invention, which are particularly useful as part of a mammalian temperature-inducible expression system. The invention also provides host cells and/or transgenic animals comprising the novel nucleic acid molecules of the invention. The invention further provides methods of using the polynucleotides of the invention, e.g., for temperature-induced transgene expression.
摘要翻译：本发明基于这样的发现，即当中国仓鼠卵巢（CHO）细胞在低于37℃的温度下培养时，仓鼠乳房肿瘤7（HMT 7）和仓鼠layilin基因的转录活性均增加。本发明提供了HMT7的新型基因组，cDNA和蛋白质序列以及仓鼠layilin的新型基因组序列。包含在基因组序列中的是各自的新型温度诱导启动子序列; 为HMT 7提供两个启动子序列，为layilin提供一个启动子序列。本发明另外为HMT7的核酸分子提供反义和siRNA分子。本发明还提供了包含本发明的新型温度诱导启动子的基因工程表达载体，其特别用作哺乳动物温度诱导型表达系统的一部分。本发明还提供了包含本发明的新型核酸分子的宿主细胞和/或转基因动物。本发明进一步提供了使用本发明的多核苷酸的方法，例如用于温度诱导的转基因表达。

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