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1. 发明申请

WO2019236502A9 BIOMASS COMPOSITIONS 审中-公开
公开(公告)号：WO2019236502A9
公开(公告)日：2019-12-12
申请号：PCT/US2019/035262
申请日：2019-06-03
申请人： HELIAE DEVELOPMENT LLC
发明人： CARNEY, Laura , MILLER, Michael , RIAL, Amy , OSGOOD, Connor
IPC分类号： A01N65/03 , A01P21/00
摘要： The present invention generally relates to agriculture and, more specifically, to biomass compositions and methods for decreasing bruising, increasing plant health, increasing soil health, increasing sweetness in fruits and/or decreasing needle-drop in conifer species.

2. 发明申请

WO2019126652A1 ANAPLEROTIC OIL PRODUCTION IN MICROBIALS 审中-公开
公开(公告)号：WO2019126652A1
公开(公告)日：2019-06-27
申请号：PCT/US2018/067104
申请日：2018-12-21
申请人： HELIAE DEVELOPMENT, LLC
发明人： TABERNA, Eneko Ganuza , ERNULT, Magdalena Amezquita , SELLERS, Charles
IPC分类号： C12P7/64 , C11C3/10
CPC分类号： C12P7/6409 , C11B1/025 , C11B1/10 , C11C3/10 , C12P7/64
摘要： Disclosed are techniques and systems for producing microbials having anaplerotic oils that are rich in odd-chain fatty acids, and other beneficial components, at higher concentrations than those present in other natural dietary sources of OCFA, at lower cost, and higher production yield. Such compositions can comprise pentadecanoic and heptadecanoic fatty acids. The techniques described herein include methods for producing and deriving such compositions rich in odd-chain fatty acids from microbials, including microalgae and yeasts/fungi.

3. 发明申请

WO2018156843A1 LAMINARIA BASED COMPOSITIONS FOR BENEFITTING PLANTS AND METHODS OF APPLICATION 审中-公开
公开(公告)号：WO2018156843A1
公开(公告)日：2018-08-30
申请号：PCT/US2018/019345
申请日：2018-02-23
申请人： HELIAE DEVELOPMENT, LLC
发明人： SHINDE, Sandip , WARNER, Michael
IPC分类号： A01P21/00 , A01N65/03 , A01N65/00
摘要： One or more compositions and methods for applying compositions, for improving characteristics of plants and soil are disclosed. The methods can be utilized by administering an effective amount of a macroalgal based composition (e.g., algal composition), such as comprising an extract of the biomass of an algae. Low concentration of the composition may be used for the application, to provide effective results.

4. 发明申请

WO2017218896A1 MICROALGAE-BASED COMPOSITION, AND METHODS OF ITS PREPARATION AND APPLICATION TO PLANTS 审中-公开
标题翻译：基于微藻的组合物及其在植物上的制备和应用方法
公开(公告)号：WO2017218896A1
公开(公告)日：2017-12-21
申请号：PCT/US2017/037878
申请日：2017-06-16
申请人： HELIAE DEVELOPMENT, LLC
发明人： SHINDE, Sandip , CARNEY, Laura , TABERNA, Eneko Ganuza , CIZEK, Luke , HANSEN, Jon , CHELLAPPAN, Ganapathy , BENNETT, Braden , SORENSEN, Kristine , VENTRE, Stephen , DONOWITZ, Nicholas Adam , ROHLFSEN, Michael Clint
IPC分类号： A01N63/00 , A01N59/04
摘要： A liquid phototrophic, mixotrophic, or heterotropic microalgae-based composition and methods preparing a liquid microalgae-based composition that can include pasteurization and stabilization of a low concentration of microalgae whole cells that have not been subjected to a drying process are disclosed. The liquid composition can be used to enhance the emergence and growth of plants in low concentration and low frequency soil and foliar applications.
摘要翻译：基于液体光养，混合营养或异养微藻的组合物和制备基于液体微藻的组合物的方法，所述组合物可包括巴氏杀菌和稳定未经受过滤的低浓度微藻全细胞公开了干燥过程。液体组合物可以用于增强植物在低浓度和低频率土壤和叶面施用中的出现和生长。

5. 发明申请

WO2013142694A1 METHOD OF EXTRACTING NEUTRAL LIPIDS WITH TWO SOLVENTS 审中-公开
标题翻译：用两种溶剂萃取中性脂肪酸的方法
公开(公告)号：WO2013142694A1
公开(公告)日：2013-09-26
申请号：PCT/US2013/033311
申请日：2013-03-21
申请人： HELIAE DEVELOPMENT, LLC
发明人： KALE, Aniket
IPC分类号： B01D11/02 , C11B7/00
CPC分类号： B01D11/0234 , B01D11/0203 , B01D11/028 , B01D11/0288 , C11B1/10 , C11B1/108 , C11C3/003
摘要： A method for separating neutral lipids from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting neutral lipids from the algal cells. The methods provide for single and multistep extraction processes which allow for efficient separation of algal neutral lipids from a wet algal biomass while avoiding emulsification of extraction mixtures. These neutral lipids are high value products which can be used to generate renewable fuels.
摘要翻译：使用两亲性溶剂组和疏水性溶剂组从植物材料，特别是完整的藻类细胞中分离中性脂质的方法。一些实施方案包括将完整的藻类细胞脱水，然后从藻类细胞中提取中性脂质。该方法提供单步和多步提取方法，其允许藻类中性脂质与湿藻类生物质的有效分离，同时避免提取混合物的乳化。这些中性脂质是可用于生产可再生燃料的高价值产品。

6. 发明申请

WO2011127171A1 METHODS OF AND SYSTEMS FOR PRODUCING BIOFUELS 审中-公开
标题翻译：生产生物燃料的方法和系统
公开(公告)号：WO2011127171A1
公开(公告)日：2011-10-13
申请号：PCT/US2011/031419
申请日：2011-04-06
申请人： HELIAE DEVELOPMENT, LLC , KALE, Aniket
发明人： KALE, Aniket
IPC分类号： C11B1/10 , C12P7/64
CPC分类号： C11B1/10 , B01D11/04 , C11B1/108 , Y02E50/13
摘要： A method for producing biofuels is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, extracting neutral lipids from the algal biomass, and esterifying the neutral lipids with a catalyst in the presence of an alcohol. The method also includes separating a water soluble fraction comprising glycerin from a water insoluble fraction comprising fuel esters and distilling the fuel esters under vacuum to obtain a C 16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising carotenoids and omega-3 fatty acids. The method further includes hydrogenating and deoxygenating at least one of (i) the C16 or shorter fuel esters to obtain a jet fuel blend stock and (ii) the C 16 or longer fuel esters to obtain a diesel blend stock.
摘要翻译：提供生产生物燃料的方法。制造生物燃料的方法包括使基本上完整的藻细胞脱水以制备藻类生物质，从藻类生物质中提取中性脂质，并在醇存在下用催化剂酯化中性脂质。该方法还包括从包含燃料酯的水不溶性部分中分离包含甘油的水溶性馏分，并在真空下蒸馏燃料酯以获得C 16或更短的燃料酯馏分，C16或更长的燃料酯馏分，以及包含类胡萝卜素和ω-3脂肪酸。该方法还包括将（i）C16或更短的燃料酯中的至少一种氢化和脱氧以获得喷气燃料共混料和（ii）C 16或更长的燃料酯以获得柴油混合原料。

7. 发明申请

WO2011127169A1 METHODS OF AND SYSTEMS FOR ISOLATING CAROTENOIDS AND OMEGA- 3 RICH OILS FROM ALGAE 审中-公开
标题翻译：用于分离来自阿尔卑斯的卡介质和OMEGA- 3多种油的方法和系统
公开(公告)号：WO2011127169A1
公开(公告)日：2011-10-13
申请号：PCT/US2011/031417
申请日：2011-04-06
申请人： HELIAE DEVELOPMENT, LLC , KALE, Aniket
发明人： KALE, Aniket
IPC分类号： C11B1/10 , C12P7/64
CPC分类号： C11B1/10 , B01D11/04 , C11B1/108 , Y02E50/13
摘要： A method of isolating nutraceuticals products from algae is provided. A method of isolating carotenoids and omega-3 rich oil from algae includes dewatering substantially intact algal cells to make an algal biomass and adding a first ethanol fraction to the algal biomass. The method also includes separating a first substantially solid biomass fraction from a first substantially liquid fraction comprising proteins and combining the first substantially solid biomass fraction with a second ethanol fraction. The method further includes separating a second substantially solid biomass fraction from a second substantially liquid fraction comprising polar lipids and combining the second substantially solid biomass fraction with a third ethanol solvent fraction. The method also includes separating a third substantially solid biomass fraction from a third substantially liquid fraction comprising neutral lipids, wherein the third substantially solid biomass fraction comprises carbohydrates and separating the neutral lipids into carotenoids and omega-3 rich oil.
摘要翻译：提供了一种从藻类中分离营养品的方法。从藻类中分离类胡萝卜素和富含ω-3的油的方法包括将基本上完整的藻类细胞脱水以制备藻类生物质并向藻类生物质中加入第一乙醇级分。该方法还包括从包含蛋白质的第一基本上液体的馏分中分离第一基本上固体的生物质馏分并将第一基本上固体的生物质馏分与第二乙醇馏分组合。该方法还包括从包含极性脂质的第二基本上液体的部分中分离第二基本上固体的生物质馏分，并将第二基本上固体的生物质馏分与第三乙醇溶剂馏分组合。该方法还包括从包含中性脂质的第三基本上液体的级分分离第三基本上固体的生物质馏分，其中第三基本上固体的生物质级分包含碳水化合物并将中性脂质分离成类胡萝卜素和富含ω3的油。

8. 发明公开

EP2917333A2 METHODS OF CULTURING MICROORGANISMS IN NON-AXENIC MIXOTROPHIC CONDITIONS AND CONTROLLING BACTERIAL CONTAMINATION IN THE CULTURES USING ACETATE AND / OR OXIDIZING AGENTS 失效
标题翻译：方法繁殖的微生物在非无菌混合营养条件和用于控制细菌污染的醋酸和/或氧化基金CULTURES
公开(公告)号：EP2917333A2
公开(公告)日：2015-09-16
申请号：EP13795380.8
申请日：2013-11-08
申请人： Heliae Development LLC
发明人： GANUZA, Eneko , LICAMELE, Jason, David , TONKOVICH, Anna, Lee
IPC分类号： C12N1/12 , A01G33/00
CPC分类号： C12N1/12 , A01G33/00
摘要： Methods of culturing microorganisms in non-axenic mixotrophic conditions are disclosed. A method of culturing microalgae mixotrophically and controlling bacterial contamination with an acetic acid/pH auxostat system is specifically described. Methods of culturing microalgae mixotrophically with an increased productivity through an increase in oxygen transfer to the culture, and controlling bacterial contamination with an oxidative agent are also described.
摘要翻译：在非无菌条件下混合营养培养微生物的方法是游离缺失盘。 mixotrophically培养微藻和控制与乙酸/ pH值auxostat系统细菌污染的方法进行具体说明。 mixotrophically与提高生产率通过如此描述中的氧转移到增加至培养培养微藻的方法。

9. 发明授权

EP2556137B1 METHODS OF AND SYSTEMS FOR PRODUCING BIOFUELS 有权
标题翻译：方法和系统，用于生物燃料生产
公开(公告)号：EP2556137B1
公开(公告)日：2015-06-03
申请号：EP11715139.9
申请日：2011-04-06
申请人： Heliae Development LLC
发明人： KALE, Aniket
IPC分类号： C11B1/10 , C12P7/64
CPC分类号： C11B1/10 , B01D11/04 , C11B1/108 , Y02E50/13

10. 发明公开

EP2556136A1 METHODS OF AND SYSTEMS FOR ISOLATING CAROTENOIDS AND OMEGA- 3 RICH OILS FROM ALGAE 有权
标题翻译：方法和系统类胡萝卜素的分离及OMEGA-3丰富的石油从藻类
公开(公告)号：EP2556136A1
公开(公告)日：2013-02-13
申请号：EP11715138.1
申请日：2011-04-06
申请人： Heliae Development LLC
发明人： KALE, Aniket
IPC分类号： C11B1/10 , C12P7/64
CPC分类号： C11B1/10 , B01D11/04 , C11B1/108 , Y02E50/13
摘要： A method of isolating nutraceuticals products from algae is provided. A method of isolating carotenoids and omega-3 rich oil from algae includes dewatering substantially intact algal cells to make an algal biomass and adding a first ethanol fraction to the algal biomass. The method also includes separating a first substantially solid biomass fraction from a first substantially liquid fraction comprising proteins and combining the first substantially solid biomass fraction with a second ethanol fraction. The method further includes separating a second substantially solid biomass fraction from a second substantially liquid fraction comprising polar lipids and combining the second substantially solid biomass fraction with a third ethanol solvent fraction. The method also includes separating a third substantially solid biomass fraction from a third substantially liquid fraction comprising neutral lipids, wherein the third substantially solid biomass fraction comprises carbohydrates and separating the neutral lipids into carotenoids and omega-3 rich oil.

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