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1. 发明授权

US09200334B2 Cell sorter system and method 有权
标题翻译：细胞分选系统和方法
公开(公告)号：US09200334B2
公开(公告)日：2015-12-01
申请号：US14009032
申请日：2012-03-13
申请人： Ger van den Engh
发明人： Ger van den Engh
IPC分类号： G01N15/14 , C12Q3/00 , C12M1/00 , C12N13/00 , G01N15/10 , B01L3/00
CPC分类号： G01N15/14 , B01L3/502715 , C12M47/04 , C12N13/00 , C12Q3/00 , G01N15/1012 , G01N15/1031 , G01N2015/0065 , G01N2015/1006 , G01N2015/1081 , G01N2015/149
摘要： Provided herein are improved cell sorter systems and methods. Such systems and methods provide a self-stabilizing sorter jet to automate calibration, and address the issue of drift in cell sorting systems. The systems and methods presented make it possible to determine and set the charge delay interval automatically with circuitry in the cell sorter. These circuits can set, monitor, and adjust the time delay continuously, allowing for a completely automatic, autonomous, turn-key, self-stabilizing sorter jet.
摘要翻译：本文提供了改进的细胞分选机系统和方法。这种系统和方法提供自稳定分选机射流以自动校准，并解决细胞分选系统中漂移的问题。所提出的系统和方法使得可以自动确定和设置电池分选器中的电路的充电延迟时间。这些电路可以连续设置，监视和调整时间延迟，从而允许一个完全自动的，自主的，交钥匙的自稳定分拣机。

2. 发明申请

US20140051064A1 Cell Sorter System and Method 有权
标题翻译：细胞分选系统和方法
公开(公告)号：US20140051064A1
公开(公告)日：2014-02-20
申请号：US14009032
申请日：2012-03-13
申请人： Ger van den Engh
发明人： Ger van den Engh
IPC分类号： C12Q3/00
CPC分类号： G01N15/14 , B01L3/502715 , C12M47/04 , C12N13/00 , C12Q3/00 , G01N15/1012 , G01N15/1031 , G01N2015/0065 , G01N2015/1006 , G01N2015/1081 , G01N2015/149
摘要： Provided herein are improved cell sorter systems and methods. Such systems and methods provide a self-stabilizing sorter jet to automate calibration, and address the issue of drift in cell sorting systems. The systems and methods presented make it possible to determine and set the charge delay interval automatically with circuitry in the cell sorter. These circuits can set, monitor, and adjust the time delay continuously, allowing for a completely automatic, autonomous, turn-key, self-stabilizing sorter jet.
摘要翻译：本文提供了改进的细胞分选机系统和方法。这种系统和方法提供自稳定分选机射流以自动校准，并解决细胞分选系统中漂移的问题。所提出的系统和方法使得可以自动确定和设置电池分选器中的电路的充电延迟时间。这些电路可以连续设置，监视和调整时间延迟，从而允许一个完全自动的，自主的，交钥匙的自稳定分拣机。

3. 发明授权

US5726364A Sample introduction apparatus for a flow cytometer 失效
标题翻译：用于流式细胞仪的样品引入装置
公开(公告)号：US5726364A
公开(公告)日：1998-03-10
申请号：US798177
申请日：1997-02-10
申请人： Ger Van den Engh
发明人： Ger Van den Engh
IPC分类号： G01N1/00 , G01N15/14 , G01N35/10
CPC分类号： G01N15/1404 , G01N35/1095
摘要： A sample introduction system for a flow cytometer allows easy change of sample containers such as test tubes and facilitates use in high pressure environments. The sample container includes a cap having a pressure supply chamber and a sample container attachment cavity. A sample container may be automatically positioned into the attachment cavity so as to sealably engage the end of the sample container as its outer surface. This positioning may be accomplished through some sample introduction mechanism. To facilitate cleaning HPLC tubing and fittings may be used in a manner which facilitates removable of the entire tubing from both the nozzle container and other sample container cap to permit its replacement to avoid contamination. The sample container support may include horizontal stops which loosely limit the movement of the sample container and thus avoid further stresses upon it.
摘要翻译：用于流式细胞仪的样品引入系统允许容易地更换样品容器，例如试管，并且便于在高压环境中使用。样品容器包括具有压力供应室和样品容器附着腔的盖。样品容器可以自动地定位到附件空腔中，以便与其外表面密封地接合样品容器的端部。这种定位可以通过一些样品引入机制来实现。为了方便清洗，HPLC管道和接头可以以便于从喷嘴容器和其他样品容器盖两者移除整个管道的方式使用，以允许其更换以避免污染。样品容器支撑件可以包括水平止挡件，其松散地限制了样品容器的移动，并因此避免了其上的进一步的压力。

4. 发明授权

US07923252B2 Droplet formation systems for flow cytometers 有权
标题翻译：流式细胞仪的液滴形成系统
公开(公告)号：US07923252B2
公开(公告)日：2011-04-12
申请号：US11069529
申请日：2005-02-28
申请人： Ger Van den Engh
发明人： Ger Van den Engh
IPC分类号： G01N21/85 , G01N33/48
CPC分类号： G01N15/1404 , G01N2015/1406 , G01N2015/1409 , Y10T436/25375 , Y10T436/2575
摘要： A droplet forming flow cytometer system (1) allows high speed processing without the need for high oscillator drive powers through the inclusion of an oscillator or piezoelectric crystal (10) within the nozzle volume (3) and directly coupled to the sheath fluid. The nozzle container (27) continuously converges so as to amplify unidirectional oscillations (11) which are transmitted as pressure waves through the nozzle volume (3) to the nozzle exit so as to form droplets from the fluid jet. A variation in substance concentration is achieved through a movable substance introduction port (9) which is positioned within a convergence zone (32) to vary the relative concentration of substance to sheath fluid while still maintaining optimal laminar flow conditions. This variation may be automatically controlled through a sensor and controller configuration.
摘要翻译：液滴形成流式细胞仪系统（1）允许高速处理，而不需要通过在喷嘴体积（3）内包含振荡器或压电晶体（10）并且直接耦合到鞘液的高振荡器驱动功率。喷嘴容器（27）连续会聚，以便放大单向振荡（11），其作为压力波通过喷嘴容积（3）传递到喷嘴出口，以便从流体射流形成液滴。通过位于会聚区（32）内的可移动物质引入口（9）来实现物质浓度的变化，以改变物质与鞘液的相对浓度，同时仍保持最佳的层流条件。这种变化可以通过传感器和控制器配置自动控制。

5. 发明授权

US5602039A Flow cytometer jet monitor system 失效
标题翻译：流式细胞仪喷射监测系统
公开(公告)号：US5602039A
公开(公告)日：1997-02-11
申请号：US323352
申请日：1994-10-14
申请人： Ger Van den Engh
发明人： Ger Van den Engh
IPC分类号： G01N15/10 , G01N15/14
CPC分类号： G01N15/1404 , G01N2015/1406 , G01N2015/149
摘要： A direct jet monitor illuminates the jet of a flow cytometer in a monitor wavelength band which is substantially separate from the substance wavelength band. When a laser is used to cause fluorescence of the substance, it may be appropriate to use an infrared source to illuminate the jet and thus optically monitor the conditions within the jet through a CCD camera or the like. This optical monitoring may be provided to some type of controller or feedback system which automatically changes either the horizontal location of the jet, the point at which droplet separation occurs, or some other condition within the jet in order to maintain optimum conditions. The direct jet monitor may be operated simultaneously with the substance property sensing and analysis system so that continuous monitoring may be achieved without interfering with the substance data gathering and may be configured so as to allow the front of the analysis or free fall area to be unobstructed during processing.
摘要翻译：直接喷射监测器照射与物质波长带基本分离的监测波长带中的流式细胞仪的射流。当使用激光来引起物质的荧光时，使用红外光源来照射射流并因此通过CCD照相机等光学地监测射流内的状况可能是合适的。该光学监视可以被提供给某种类型的控制器或反馈系统，其自动地改变射流的水平位置，发生液滴分离的点，或喷射内的某些其他状态，以便保持最佳条件。直接喷射监测器可以与物质性质感测和分析系统同时操作，使得可以在不干扰物质数据收集的情况下实现连续监测，并且可以将其配置成允许分析或自由落体区域的前部不受阻碍在处理过程中。

6. 发明授权

US08223445B2 Lens positioning apparatus 有权
标题翻译：镜头定位装置
公开(公告)号：US08223445B2
公开(公告)日：2012-07-17
申请号：US12872869
申请日：2010-08-31
申请人： Ger van den Engh
发明人： Ger van den Engh
IPC分类号： G02B7/02
CPC分类号： G01N15/14
摘要： The present invention provides an optical analyzer having illumination optics that include a light source, such as a laser or other source, adapted to emit a collimated, or approximately collimated, light beam, and a focusing lens that focuses the beam onto a focus spot within a detection region, wherein the focusing lens is mounted in a lens positioning apparatus that allows for precise positioning of the focus spot within the detection region. The lens positioning apparatus comprises a lens holder adapted to rotate through a small angle around a pivot axis parallel to the optical path, such that the lens holder rotates in a plane perpendicular to the optical path, and an actuator adapted to provide an angular displacement of the lens holder around the pivot axis. The lens holder holds the focusing lens at a first distance from the pivot axis, and is coupled to the actuator at a second distance from the pivot axis, wherein the second distance is larger than the first distance.
摘要翻译：本发明提供了一种具有照明光学器件的光学分析器，其包括适于发射准直或近似准直的光束的光源，例如激光器或其他光源，以及将光束聚焦到焦点上的聚焦点检测区域，其中聚焦透镜安装在允许焦点在检测区域内精确定位的透镜定位装置中。透镜定位装置包括透镜保持器，其适于围绕平行于光路的枢转轴线以小角度旋转，使得透镜保持器在垂直于光路的平面中旋转，并且致动器适于提供透镜支架围绕枢转轴线。镜头保持器将聚焦透镜保持在距离枢转轴线的第一距离处，并且与枢转轴线相距第二距离处联接到致动器，其中第二距离大于第一距离。

7. 发明申请

US20110069492A1 LENS POSITIONING APPARATUS 有权
标题翻译：镜头定位装置
公开(公告)号：US20110069492A1
公开(公告)日：2011-03-24
申请号：US12872869
申请日：2010-08-31
申请人： Ger van den Engh
发明人： Ger van den Engh
IPC分类号： F21V5/04
CPC分类号： G01N15/14
摘要： The present invention provides an optical analyzer having illumination optics that include a light source, such as a laser or other source, adapted to emit a collimated, or approximately collimated, light beam, and a focusing lens that focuses the beam onto a focus spot within a detection region, wherein the focusing lens is mounted in a lens positioning apparatus that allows for precise positioning of the focus spot within the detection region. The lens positioning apparatus comprises a lens holder adapted to rotate through a small angle around a pivot axis parallel to the optical path, such that the lens holder rotates in a plane perpendicular to the optical path, and an actuator adapted to provide an angular displacement of the lens holder around the pivot axis. The lens holder holds the focusing lens at a first distance from the pivot axis, and is coupled to the actuator at a second distance from the pivot axis, wherein the second distance is larger than the first distance.
摘要翻译：本发明提供了一种具有照明光学器件的光学分析器，其包括适于发射准直或近似准直的光束的光源，例如激光器或其他光源，以及将光束聚焦到焦点上的聚焦点检测区域，其中聚焦透镜安装在允许焦点在检测区域内精确定位的透镜定位装置中。透镜定位装置包括透镜保持器，其适于围绕平行于光路的枢转轴线以小角度旋转，使得透镜保持器在垂直于光路的平面中旋转，并且致动器适于提供透镜支架围绕枢转轴线。镜头保持器将聚焦透镜保持在距离枢转轴线的第一距离处，并且与枢转轴线相距第二距离处联接到致动器，其中第二距离大于第一距离。

8. 发明申请

US20080316481A1 APPARATUS FOR ANALYZING AND SORTING BIOLOGICAL PARTICLES 有权
标题翻译：用于分析和分类生物颗粒的装置
公开(公告)号：US20080316481A1
公开(公告)日：2008-12-25
申请号：US12016856
申请日：2008-01-18
申请人： Ger van den Engh
发明人： Ger van den Engh
IPC分类号： G01J3/30 , G01N21/00
CPC分类号： G01N15/1434 , G01N2015/149
摘要： The invention provides an apparatus including (a) a frame having a boundary plane; (b) a flow chamber supported by the frame, the flow chamber placed a distance from the boundary plane; (c) a radiation source, the radiation source directed away from the flow chamber and away from the exterior side of the boundary plane, and (d) a first reflective surface placed to direct a radiation beam in a path crossing the boundary plane to the flow chamber; (e) one or more reflective surfaces placed to direct a radiation beam from the radiation source to the first reflective surface, the path from the radiation source to the flow chamber being at least 1.5 times the distance from the flow chamber to the boundary plane.
摘要翻译：本发明提供一种装置，包括：（a）具有边界平面的框架; （b）由框架支撑的流动室，流动室与边界平面放置一段距离; （c）辐射源，所述辐射源指向远离所述流动室且远离所述边界平面的外侧，以及（d）第一反射表面，其设置成将辐射束引导到与所述边界平面相交的路径中流动室; （e）放置成将辐射束从辐射源引导到第一反射表面的一个或多个反射表面，从辐射源到流动室的路径距流动室至边界平面的距离的至少1.5倍。

9. 发明授权

US6133044A High speed flow cytometer droplet formation system and method 失效
标题翻译：高速流式细胞仪液滴形成系统及方法
公开(公告)号：US6133044A
公开(公告)日：2000-10-17
申请号：US627963
申请日：1996-04-16
申请人： Ger Van den Engh
发明人： Ger Van den Engh
IPC分类号： G01N15/14 , G01N1/18
CPC分类号： G01N15/1404 , G01N2015/1406 , G01N2015/1409 , Y10T436/117497 , Y10T436/118339 , Y10T436/25375 , Y10T436/2575
摘要： A droplet forming flow cytometer system allows high speed processing without the need for high oscillator drive powers through the inclusion of an oscillator or piezoelectric crystal such as within the nozzle volume or otherwise unidirectionally coupled to the sheath fluid. The nozzle container continuously converges so as to amplify unidirectional oscillations which are transmitted as pressure waves through the nozzle volume to the nozzle exit so as to form droplets from the fluid jet. The oscillator is directionally isolated so as to avoid moving the entire nozzle container so as to create only pressure waves within the sheath fluid. A variation in substance concentration is achieved through a movable substance introduction port which is positioned within a convergence zone to vary the relative concentration of substance to sheath fluid while still maintaining optimal laminar flow conditions. This variation may be automatically controlled through a sensor and controller configuration. A replaceable tip design is also provided whereby the ceramic nozzle tip is positioned within an edge insert in the nozzle body so as to smoothly transition from nozzle body to nozzle tip. The nozzle tip is sealed against its outer surface to the nozzle body so it may be removable for cleaning or replacement.
摘要翻译：液滴形成流式细胞仪系统允许高速处理，而不需要通过包括振荡器或压电晶体（例如在喷嘴体积内）或以其它方式单独耦合到鞘液的高振荡器驱动功率。喷嘴容器连续会聚，以便放大单向振荡，其作为压力波通过喷嘴体积传递到喷嘴出口，以便从流体射流形成液滴。振荡器是定向隔离的，以避免移动整个喷嘴容器，从而仅在护套液体内产生压力波。通过位于会聚区内的可移动物质引入口来实现物质浓度的变化，以改变物质与鞘液的相对浓度，同时仍保持最佳的层流条件。这种变化可以通过传感器和控制器配置自动控制。还提供了可替换的尖端设计，其中陶瓷喷嘴尖端位于喷嘴体内的边缘插入件中，以便从喷嘴体平滑地过渡到喷嘴尖端。喷嘴尖端与其外表面密封到喷嘴体，因此其可以是可移除的用于清洁或更换。

10. 发明授权

US5483469A Multiple sort flow cytometer 失效
标题翻译：多重流式细胞仪
公开(公告)号：US5483469A
公开(公告)日：1996-01-09
申请号：US100762
申请日：1993-08-02
申请人： Ger Van den Engh , Richard J. Esposito
发明人： Ger Van den Engh , Richard J. Esposito
IPC分类号： G01N15/14 , B07C5/344
CPC分类号： G01N15/14 , G01N2015/149
摘要： A flow cytometer utilizes multiple lasers for excitation and respective fluorescence of identified dyes bonded to specific cells or events to identify and verify multiple events to be sorted from a sheath flow and droplet stream. Once identified, verified and timed in the sheath flow, each event is independently tagged upon separation from the flow by an electrical charge of +60, +120, or +180 volts and passed through oppositely charged deflection plates with ground planes to yield a focused six way deflection of at least six events in a narrow plane.
摘要翻译：流式细胞仪利用多个激光器激发和识别结合到特定细胞或事件的识别染料的相应荧光，以鉴定和验证从鞘流和液滴流中分选的多个事件。一旦识别，验证并定时在鞘流中，每个事件在+ 60，+120或+180伏的电荷与流动分离时被独立地标记，并通过带有接地平面的带相反电荷的偏转板，以产生聚焦在狭窄的平面中至少六次事件的六路偏转。

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