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1. 发明申请

US20070199105A1 UDP-Xylose Synthases (UXS) Polynucleotides, Polypeptides, and Uses Thereof 失效
标题翻译： UDP-木糖合酶（UXS）多核苷酸，多肽及其用途
公开(公告)号：US20070199105A1
公开(公告)日：2007-08-23
申请号：US11614098
申请日：2006-12-21
申请人： Xiaoming Bao , George Singletary , Deborah Wetterberg , Ramesh Nair , Kanwarpal Dhugga , Matthias Liebergesell , David Selinger
发明人： Xiaoming Bao , George Singletary , Deborah Wetterberg , Ramesh Nair , Kanwarpal Dhugga , Matthias Liebergesell , David Selinger
IPC分类号： A01H5/00 , C12N15/82 , C12N5/04
CPC分类号： C12N9/88 , C12N15/8245 , C12N15/8246 , C12N15/8261 , C12Y401/01035 , Y02A40/146
摘要： The present disclosure concerns methods and compositions relating to UXS polypeptides and/or nucleic acids encoding UXS polypeptides. In certain claims, the methods and compositions are of use to improve digestibility and/or ease of grain processing. Such improvements relate to a modulation in arabinoxylan and/or hemicellulose content in transgenic plants. Such plants can, for example, comprise one or more nucleic acid sequences that inhibit expression of one or more UDP-Xylose Synthase (UXS) genes.
摘要翻译：本公开涉及与UXS多肽和/或编码UXS多肽的核酸相关的方法和组合物。在某些权利要求中，所述方法和组合物可用于改善消化率和/或颗粒加工的易于使用。这种改进涉及转基因植物中阿拉伯木聚糖和/或半纤维素含量的调节。这样的植物可以例如包含抑制一种或多种UDP-木糖合酶（UXS）基因表达的一种或多种核酸序列。

2. 发明授权

US06232529B1 Methods of producing high-oil seed by modification of starch levels 失效
标题翻译：通过改性淀粉水平生产高油种子的方法
公开(公告)号：US06232529B1
公开(公告)日：2001-05-15
申请号：US08752503
申请日：1996-11-20
申请人： George Singletary , Paul C. Anderson , Sean J. Coughlan
发明人： George Singletary , Paul C. Anderson , Sean J. Coughlan
IPC分类号： A01H100
CPC分类号： C12N15/8207 , C12N15/8245 , C12N15/8247
摘要： The present invention provides methods of obtaining a greater than normal concentration of oil in the seed of a plant, the method comprising altering the carbon metabolism in the seed leading to an alteration in the partitioning of assimilates from starch biosynthesis to enhance the biosynthesis and accumulation of oil, preferably without significantly affecting the size of the storage organ in a way that adversely impacts the commercial value of the material. Seeds produced by the present methods are also provided.
摘要翻译：本发明提供在植物种子中获得大于正常浓度的油的方法，所述方法包括改变种子中的碳代谢，导致同化物与淀粉生物合成分配的改变，以增强生物合成和积累油，优选不会以不利地影响材料的商业价值的方式显着影响储存器官的尺寸。还提供了通过本发明方法生产的种子。

3. 发明授权

US6013861A Plants and processes for obtaining them 失效
标题翻译：获取它们的植物和过程
公开(公告)号：US6013861A
公开(公告)日：2000-01-11
申请号：US735491
申请日：1996-10-23
申请人： Colin R. Bird , Philip A. Fentem , Peter L. Keeling , George Singletary
发明人： Colin R. Bird , Philip A. Fentem , Peter L. Keeling , George Singletary
IPC分类号： C12N15/82 , C12N15/00 , A01H1/06
CPC分类号： C12N15/8245
摘要： Plants, particularly cereal plants, which have improved ability to synthesise starch at elevated or lowered temperatures and/or to synthesise starch with an altered fine structure are produced by inserting into the genome of the plant (i) a gene(s) encoding a form of an enzyme of the starch or glycogen biosynthetic pathway, particularly soluble starch synthase and/or branching enzyme and/or glycogen synthase, which display an activity which continues to increase over a temperature range over which the activity would normally be expected to decrease, and/or (ii) a gene(s) encoding sense and anti-sense constructs of enzymes of the starch biosynthetic pathway, particularly soluble starch synthase and/or branching enzyme and/or glycogen synthase, which alters the natural ratios of expression of the said enzymes or inserts enzymes with special structural characteristics which alter the natural branching pattern in starch.
摘要翻译：通过将植物的基因组插入到植物的基因组中（i）编码形式的基因来产生具有改善的在升高或降低的温度下合成淀粉和/或合成具有改变的精细结构的淀粉的植物，特别是谷物植物的淀粉或糖原生物合成途径的酶，特别是可溶性淀粉合成酶和/或分支酶和/或糖原合成酶，其显示在通常预期降低的活性的温度范围内持续增加的活性，以及 /或（ii）编码淀粉生物合成途径的酶的有义和反义构建体的基因，特别是可溶性淀粉合成酶和/或分支酶和/或糖原合成酶，其改变所述酶或插入具有改变淀粉中天然分支模式的特殊结构特征的酶。

4. 发明申请

US20070089200A1 Manipulating the expression of reversibly glycosylated polypeptide (RGP) in plants 审中-公开
标题翻译：在植物中操纵可逆糖基化多肽（RGP）的表达
公开(公告)号：US20070089200A1
公开(公告)日：2007-04-19
申请号：US11512956
申请日：2006-08-29
申请人： Xiaoming Bao , Darren Gruis , Craig Hastings , George Singletary , Deborah Wetterberg
发明人： Xiaoming Bao , Darren Gruis , Craig Hastings , George Singletary , Deborah Wetterberg
IPC分类号： A01H5/00 , C12N15/82 , C12N5/04 , A01H1/00
CPC分类号： C12N15/8245 , C12N15/8246 , C12N15/8247 , C12N15/8251 , C12N15/8261 , Y02A40/146
摘要： Compositions and methods for altering the levels of reversibly glycosylated polypeptide (RGP) in plants are provided. The present invention recognizes that by altering the level of expression of RGP, plants and seeds having beneficial qualities can be obtained. The invention involves controlled suppression of RGP expression to obtain plants and seeds having decreased amounts of hemicellulose and arabinose. Controlled suppression of RGP, particularly in vegetative tissues and seeds, leads to plants with desirable characteristics, including altered cell wall structure, reduced grain fiber content, increased seed protein, increased oil content, and altered arabinoxylan or xyloglucan levels. Compositions of the invention include constructs for the suppression of RGP and transformed plants and seeds having the altered phenotype of the invention.
摘要翻译：提供了用于改变植物中可逆糖基化多肽（RGP）水平的组合物和方法。本发明认识到，通过改变RGP的表达水平，可以获得具有有益品质的植物和种子。本发明涉及RGP表达的受控抑制以获得具有减少量的半纤维素和阿拉伯糖的植物和种子。 RGP的受控抑制，特别是在营养组织和种子中，导致具有所需特征的植物，包括改变的细胞壁结构，减少的谷物纤维含量，增加的种子蛋白，增加的油含量和改变的阿糖木聚糖或木葡聚糖水平。本发明的组合物包括用于抑制RGP的转化植物和具有改变的本发明表型的转化植物和种子的构建体。

5. 发明申请

US20140068817A1 EXPRESSION OF ISOMERS OF SUCROSE INCREASES SEED WEIGHT, SEED NUMBER AND/OR SEED SIZE 审中-公开
标题翻译：种子的表达增加了种子重量，种子数和/或种子大小
公开(公告)号：US20140068817A1
公开(公告)日：2014-03-06
申请号：US14002823
申请日：2012-03-05
申请人： Stefan Dittmar , George Singletary , Asha Palta , Barry Stoddard , Paul Olivier
发明人： Stefan Dittmar , George Singletary , Asha Palta , Barry Stoddard , Paul Olivier
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C12N9/90 , Y02A40/146
摘要： The present invention provides expression vectors comprising polynucleotides encoding chimeric sucrose isomerases, and methods of using the same. In addition, transgenic plants expressing said chimeric sucrose isomerases are provided. Furthermore, methods of increasing average seed weight, seed number and/or seed size of a plant by using said chimeric sucrose isomerases are provided.
摘要翻译：本发明提供了包含编码嵌合蔗糖异构酶的多核苷酸的表达载体及其使用方法。此外，提供了表达所述嵌合蔗糖异构酶的转基因植物。此外，提供了通过使用所述嵌合蔗糖异构酶增加植物的平均种子重量，种子数量和/或种子大小的方法。

6. 发明申请

US20060064779A1 Modulating myo-inositol catabolism in plants 失效
标题翻译：调节植物中的肌醇分解代谢
公开(公告)号：US20060064779A1
公开(公告)日：2006-03-23
申请号：US11064295
申请日：2005-02-23
申请人： Matthias Liebergesell , Jinrui Shi , George Singletary
发明人： Matthias Liebergesell , Jinrui Shi , George Singletary
IPC分类号： A01H1/00 , C12N15/82 , C12N5/04
CPC分类号： C12N9/0069 , C12N15/8245 , C12N15/8246 , C12N15/8261 , C12N15/8267 , Y02A40/146
摘要： Compositions and methods for modulating MIOX activity are provided. Such compositions include nucleotide sequences for novel MIOX sequences obtained from maize, amino acid sequences for the proteins encoded by the nucleotide sequences of the invention, and variants and fragments thereof. Methods of the invention involve introducing into a plant a nucleotide construct comprising a MIOX nucleotide sequence operably linked to a promoter that drives expression in a plant. Expression of the novel nucleotide sequences disclosed herein confers advantageous agronomic properties on a plant. Transformed plants, plant cells, and seeds are additionally provided.
摘要翻译：提供了调节MIOX活性的组合物和方法。这样的组合物包括从玉米获得的新型MIOX序列的核苷酸序列，由本发明的核苷酸序列编码的蛋白质的氨基酸序列及其变体和片段。本发明的方法包括向植物中引入一种核苷酸构建体，该核苷酸构建体包含可操作地连接到驱动植物表达的启动子的MIOX核苷酸序列。本文公开的新型核苷酸序列的表达赋予植物有利的农学性质。另外提供转化的植物，植物细胞和种子。

7. 发明申请

US20050160496A1 Production of cereal grain with reduced starch granule size and uses thereof 失效
标题翻译：生产具有降低淀粉颗粒大小的谷物及其用途
公开(公告)号：US20050160496A1
公开(公告)日：2005-07-21
申请号：US11021464
申请日：2004-12-22
申请人： George Singletary , Lan Zhou
发明人： George Singletary , Lan Zhou
IPC分类号： C12N15/82 , A01H1/00 , C12N9/32
CPC分类号： C12N15/8245
摘要： The invention alters the physical characteristics of starch granules in a plant. Methods are provided for reducing starch granule size by stably introducing into the genome of the plant or plant cell a DNA construct comprising a polynucleotide encoding a polypeptide operably linked to a promoter that drives expression in a plant and operably linked to a plastid transit peptide. Compositions of the invention include transgenic plants and seeds having a reduced starch granule size. Compositions also include starch isolated from these plants and seeds. The modified starch granules find use in a wide range of food, feed, and industrial applications.
摘要翻译：本发明改变植物中淀粉颗粒的物理特征。提供了通过将植物或植物细胞的基因组稳定地引入到包含编码与驱动植物中表达并且可操作地连接到质体转运肽的启动子有效连接的多肽的多核苷酸的DNA构建体来提供降低淀粉颗粒大小的方法。本发明的组合物包括具有降低的淀粉颗粒大小的转基因植物和种子。组合物还包括从这些植物和种子分离的淀粉。改性淀粉颗粒用于广泛的食品，饲料和工业应用。

8. 发明申请

US20050160494A1 Alteration of oil traits in plants 审中-公开
标题翻译：改变植物油性状
公开(公告)号：US20050160494A1
公开(公告)日：2005-07-21
申请号：US11021666
申请日：2004-12-23
申请人： George Singletary , Peter Coaldrake , Paulette Krumpelman , Douglas Nubel , Court Saunders , Mitchell Tarczynski , Lan Zhou
发明人： George Singletary , Peter Coaldrake , Paulette Krumpelman , Douglas Nubel , Court Saunders , Mitchell Tarczynski , Lan Zhou
IPC分类号： A01H1/00 , C12N15/82
CPC分类号： C12N15/8245 , C12N15/8247
摘要： Compositions and methods for modulating the production and characteristics of oil in a plant or plant part thereof are provided. Compositions of the invention are nucleotide constructs that provide for expression of transcriptional activators that increase lipid biosynthesis in a plant in combination with the inhibition or disruption of starch biosynthesis and/or starch storage, and optionally in combination with expression of another polynucleotide of interest. Compositions also include transformed plants, plant cells, plant tissues, and plant seeds comprising these nucleotide constructs. The methods of the invention comprise introducing a combination of polynucleotides into a plant, wherein the polynucleotides provide for increased lipid biosynthesis and disruption of starch biosynthesis and/or starch storage, and optionally alteration of fatty acid metabolism. The methods and constructs find use in alteration of oil phenotype in a plant or plant part thereof.
摘要翻译：提供了用于调节植物或植物部分中的油的生产和特性的组合物和方法。本发明的组合物是提供转录活化剂表达的核苷酸构建体，其与植物中的脂质生物合成相结合，与淀粉生物合成和/或淀粉储存的抑制或破坏结合，以及任选地与另一感兴趣的多核苷酸的表达组合。组合物还包括转化植物，植物细胞，植物组织和包含这些核苷酸构建体的植物种子。本发明的方法包括将多核苷酸的组合引入植物中，其中所述多核苷酸提供增加的脂质生物合成和破坏淀粉生物合成和/或淀粉储存，以及任选地改变脂肪酸代谢。方法和结构可用于改变植物或植物部分的油表型。

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