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1. 发明授权

US07723573B2 Rubisco activase with increased thermostability and methods of use thereof 失效
标题翻译：具有增加的热稳定性的Rubisco活化酶及其使用方法
公开(公告)号：US07723573B2
公开(公告)日：2010-05-25
申请号：US12474298
申请日：2009-05-29
申请人： Itzhak Kurek , Lu Liu , Genhai Zhu
发明人： Itzhak Kurek , Lu Liu , Genhai Zhu
IPC分类号： C12N15/05 , A01H1/00 , C07H21/04 , C07K14/415 , C12N15/29
CPC分类号： C12N9/88 , C12N15/8261 , Y02A40/146
摘要： The present invention provides thermostable polypeptides related to Arabidopsis Rubisco Activase polypeptides. Nucleic acids encoding the polypeptides of the invention are also provided. Methods for using the polypeptides and nuclei acids of the invention to enhance resistance of plants to heat stress are encompassed.
摘要翻译：本发明提供与拟南芥Rubisco活化酶多肽相关的热稳定多肽。还提供了编码本发明多肽的核酸。包括使用本发明的多肽和核酸以增强植物对热应激的抗性的方法。

2. 发明申请

US20080271209A1 HIGH-THROUGHPUT EVALUATION OF RUBISCO AND PLANT PHOTOSYNTHETIC PERFORMANCE IN VIVO 有权
标题翻译：罗非鱼和植物光合作用的高通量评估
公开(公告)号：US20080271209A1
公开(公告)日：2008-10-30
申请号：US12110776
申请日：2008-04-28
申请人： ITZHAK KUREK , XUEHUA HU , GENHAI ZHU , LU LIU
发明人： ITZHAK KUREK , XUEHUA HU , GENHAI ZHU , LU LIU
IPC分类号： A01H5/00 , H01J49/26 , G01N30/02
CPC分类号： G01N33/50 , G01N30/02 , G01N30/7233 , Y10T436/24 , B01D15/426 , B01D15/325
摘要： The present invention provides a method for determining a level of a RuBP, PGA, 2-PA in a photosynthetic cellular extract by resolving at least one RuBP, PGA, or 2-PA or combinations thereof in a photosynthetic cellular extract obtained from a plant. In another aspect, it provides for detecting at least one resolved RuBP, PGA, or 2-PA or combinations, thereby determining a level of the resolved RuBP, PGA, or 2-PA or combinations. In one aspect the photosynthetic cellular extract is obtained from a plant subjected to an abiotic stress. A method of the invention can be useful, for example, for identifying plants with increased photosynthetic performance. Also included herein is a high throughput system for identifying a plant with increased photosynthetic performance.
摘要翻译：本发明提供了通过从植物获得的光合细胞提取物中分解至少一种RuBP，PGA或2-PA或其组合来确定光合细胞提取物中RuBP，PGA，2-PA的水平的方法。另一方面，它提供了检测至少一种分解的RuBP，PGA或2-PA或组合，从而确定分解的RuBP，PGA或2-PA或组合的水平。在一个方面，光合细胞提取物是从遭受非生物胁迫的植物获得的。本发明的方法可用于例如用于鉴定具有增加的光合性能的植物。本文还包括用于鉴定具有增加的光合性能的植物的高产量系统。

3. 发明申请

US20080072349A1 Rubisco Activase with Increased Thermostability and Methods of Use Thereof 有权
标题翻译： Rubisco活化酶具有增加的热稳定性和使用方法
公开(公告)号：US20080072349A1
公开(公告)日：2008-03-20
申请号：US11867723
申请日：2007-10-05
申请人： Itzhak Kurek , Lu Liu , Genhai Zhu
发明人： Itzhak Kurek , Lu Liu , Genhai Zhu
IPC分类号： A01H5/00 , C07H21/00 , C12N15/00 , C12N15/82 , C12N5/04 , C12N9/00
CPC分类号： C12N9/88 , C12N15/8261 , Y02A40/146
摘要： The present invention provides thermostable polypeptides related to Arabidopsis Rubisco Activase polypeptides. Nucleic acids encoding the polypeptides of the invention are also provided. Methods for using the polypeptides and nuclei acids of the invention to enhance resistance of plants to heat stress are encompassed.
摘要翻译：本发明提供与拟南芥Rubisco活化酶多肽相关的热稳定多肽。还提供了编码本发明多肽的核酸。包括使用本发明的多肽和核酸以增强植物对热应激的抗性的方法。

4. 发明申请

US20070050864A1 Rubisco activase with increased thermostability and methods of use thereof 有权
标题翻译：具有增加的热稳定性的Rubisco活化酶及其使用方法
公开(公告)号：US20070050864A1
公开(公告)日：2007-03-01
申请号：US11507729
申请日：2006-08-22
申请人： Itzhak Kurek , Lu Liu , Genhai Zhu
发明人： Itzhak Kurek , Lu Liu , Genhai Zhu
IPC分类号： A01H1/00 , C07H21/04 , C12N15/82 , C12N5/04
CPC分类号： C12N9/88 , C12N15/8261 , Y02A40/146
摘要： The present invention provides thermostable polypeptides related to Arabidopsis Rubisco Activase polypeptides. Nucleic acids encoding the polypeptides of the invention are also provided. Methods for using the polypeptides and nuclei acids of the invention to enhance resistance of plants to heat stress are encompassed.
摘要翻译：本发明提供与拟南芥Rubisco活化酶多肽相关的热稳定多肽。还提供了编码本发明多肽的核酸。包括使用本发明的多肽和核酸以增强植物对热应激的抗性的方法。

5. 发明申请

US20120304337A1 ENDOTOXINS HAVING NEMATOCIDAL ACTIVITY AND METHODS OF USE THEREOF 审中-公开
标题翻译：具有杀虫活性的内毒素及其使用方法
公开(公告)号：US20120304337A1
公开(公告)日：2012-11-29
申请号：US13478515
申请日：2012-05-23
申请人： Ute Schellenberger , Jun-Zhi Wei , Genhai Zhu
发明人： Ute Schellenberger , Jun-Zhi Wei , Genhai Zhu
IPC分类号： A01N37/46 , C12N15/32 , A01N63/02 , A01P5/00 , C12N1/13 , C12N15/82 , A01H5/00 , A01H5/10 , C12N1/21 , C12N1/19 , C07K14/325 , C12N15/63
CPC分类号： C12N15/8285 , A01N63/02 , C07K14/325 , C07K14/66 , Y02A40/164
摘要： The invention provides novel δ-endotoxin polypeptides, and variants and fragments thereof, obtained from Bacillus thuringiensis having pesticidal activity against nematodes. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, biopesticide compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant parasitic nematodes.
摘要翻译：本发明提供了从苏云金芽孢杆菌获得的具有针对线虫杀虫活性的新颖的δ-内毒素多肽及其变体和片段。本发明的具体实施方案提供编码杀虫蛋白，生物农药组合物，表达盒，以及包含本发明的核酸的转化的微生物和植物的分离的核酸。这些组合物用于防治害虫，特别是植物寄生线虫的方法。

6. 发明授权

US08110113B2 High-throughput evaluation of Rubisco and plant photosynthetic performance in vivo 有权
标题翻译： Rubisco和植物光合作用在体内的高通量评估
公开(公告)号：US08110113B2
公开(公告)日：2012-02-07
申请号：US12110776
申请日：2008-04-28
申请人： Itzhak Kurek , Xuehua Hu , Genhai Zhu , Lu Liu
发明人： Itzhak Kurek , Xuehua Hu , Genhai Zhu , Lu Liu
IPC分类号： B01D15/36
CPC分类号： G01N33/50 , G01N30/02 , G01N30/7233 , Y10T436/24 , B01D15/426 , B01D15/325
摘要： The present invention provides a method for determining a level of a RuBP, PGA, 2-PA in a photosynthetic cellular extract by resolving at least one RuBP, PGA, or 2-PA or combinations thereof in a photosynthetic cellular extract obtained from a plant. In another aspect, it provides for detecting at least one resolved RuBP, PGA, or 2-PA or combinations, thereby determining a level of the resolved RuBP, PGA, or 2-PA or combinations. In one aspect the photosynthetic cellular extract is obtained from a plant subjected to an abiotic stress. A method of the invention can be useful, for example, for identifying plants with increased photosynthetic performance. Also included herein is a high throughput system for identifying a plant with increased photosynthetic performance.
摘要翻译：本发明提供了一种通过在从植物获得的光合细胞提取物中拆分至少一种RuBP，PGA或2-PA或其组合来测定光合细胞提取物中RuBP，PGA，2-PA水平的方法。另一方面，它提供了检测至少一种分解的RuBP，PGA或2-PA或组合，从而确定分解的RuBP，PGA或2-PA或组合的水平。在一个方面，光合细胞提取物是从遭受非生物胁迫的植物获得的。本发明的方法可用于例如鉴定具有增加的光合性能的植物。本文还包括用于鉴定具有增加的光合性能的植物的高产量系统。

7. 发明申请

US20090241224A1 Rubisco Activase with Increased Thermostability and Methods of Use Thereof 失效
标题翻译： Rubisco活化酶具有增加的热稳定性和使用方法
公开(公告)号：US20090241224A1
公开(公告)日：2009-09-24
申请号：US12474298
申请日：2009-05-29
申请人： Itzhak Kurek , Lu Liu , Genhai Zhu
发明人： Itzhak Kurek , Lu Liu , Genhai Zhu
IPC分类号： C12N15/82 , C12N15/60 , C12N5/10 , C12N9/88 , A01H5/00
CPC分类号： C12N9/88 , C12N15/8261 , Y02A40/146
摘要： The present invention provides thermostable polypeptides related to Arabidopsis Rubisco Activase polypeptides. Nucleic acids encoding the polypeptides of the invention are also provided. Methods for using the polypeptides and nuclei acids of the invention to enhance resistance of plants to heat stress are encompassed.
摘要翻译：本发明提供与拟南芥Rubisco活化酶多肽相关的热稳定多肽。还提供了编码本发明多肽的核酸。包括使用本发明的多肽和核酸以增强植物对热应激的抗性的方法。

8. 发明申请

US20080256666A1 METHODS OF IDENTIFYING AND CREATING RUBISCO LARGE SUBUNIT VARIANTS WITH IMPROVED RUBISCO ACTIVITY, COMPOSITIONS AND METHODS OF USE THEREOF 有权
标题翻译：利用改进的鲁莽活动识别和创建鲁比亚大型亚音乐变体的方法，组合物及其使用方法
公开(公告)号：US20080256666A1
公开(公告)日：2008-10-16
申请号：US12102639
申请日：2008-04-14
申请人： GENHAI ZHU , ITZHAK KUREK , LU LIU
发明人： GENHAI ZHU , ITZHAK KUREK , LU LIU
IPC分类号： C12N15/60 , C12Q1/68 , C12N5/10 , A01H5/00 , C12N15/82 , C12N9/88 , C12Q1/04
CPC分类号： C12N9/88 , C12N15/8269 , C12Q1/02 , C12Q1/04 , C12Y401/01039
摘要： Methods for identifying one or more amino acid substitutions in a Rubisco large subunit polypeptide (variant) that confer increased Rubisco activity in a unicellular photosynthetic organism and transferring those substitutions to a Rubisco large subunit polypeptide of a higher plant cell are described herein. Methods and compositions for modulating plant productivity using the modified Rubisco large subunit polypeptide variants are provided. The Rubisco large subunit sequences are used in a variety of methods including increasing plant productivity in a plant. Transformed plants, plant cell, tissues, seed, and expression vectors are also provided.
摘要翻译：在本文中描述了用于鉴定赋予单细胞光合生物体中增加的Rubisco活性并将这些取代转移到较高植物细胞的Rubisco大亚基多肽的Rubisco大亚基多肽（变体）中的一个或多个氨基酸取代的方法。提供了使用修饰的Rubisco大亚基多肽变体调节植物生产力的方法和组合物。 Rubisco大亚基序列用于各种方法，包括提高植物的植物生产力。还提供了转化的植物，植物细胞，组织，种子和表达载体。

9. 发明授权

US09139843B2 Endotoxins having nematocidal activity and methods of use thereof 有权
标题翻译：具有杀线虫活性的内毒素及其使用方法
公开(公告)号：US09139843B2
公开(公告)日：2015-09-22
申请号：US13478515
申请日：2012-05-23
申请人： Ute Schellenberger , Jun-Zhi Wei , Genhai Zhu
发明人： Ute Schellenberger , Jun-Zhi Wei , Genhai Zhu
IPC分类号： A01H5/00 , C12N15/82 , C07K14/325 , A01N25/00 , A01H5/10 , C12N1/21 , C12N15/32 , C07K14/66 , A01N63/02
CPC分类号： C12N15/8285 , A01N63/02 , C07K14/325 , C07K14/66 , Y02A40/164
摘要： The invention provides novel δ-endotoxin polypeptides, and variants and fragments thereof, obtained from Bacillus thuringiensis having pesticidal activity against nematodes. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, biopesticide compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant parasitic nematodes.
摘要翻译：本发明提供了从苏云金芽孢杆菌获得的具有针对线虫杀虫活性的新颖的δ-内毒素多肽及其变体和片段。本发明的具体实施方案提供编码杀虫蛋白，生物农药组合物，表达盒，以及包含本发明的核酸的转化的微生物和植物的分离的核酸。这些组合物用于防治害虫，特别是植物寄生线虫的方法。

10. 发明申请

US20130031667A1 Anti-nematode peptides and methods of use thereof 有权
标题翻译：抗线虫肽及其使用方法
公开(公告)号：US20130031667A1
公开(公告)日：2013-01-31
申请号：US13541202
申请日：2012-07-03
申请人： Steven D. Gruver , Lu Liu , Ute Schellenberger , Jun-Zhi Wei , Wieping Xie , Genhai Zhu
发明人： Steven D. Gruver , Lu Liu , Ute Schellenberger , Jun-Zhi Wei , Wieping Xie , Genhai Zhu
IPC分类号： C12N15/82 , C07H21/04 , A01H5/10 , C12N15/63 , A01H5/00 , C12N5/10 , C07K14/195
CPC分类号： C07K14/195 , A01N37/46 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： The invention provides novel polypeptides, and variants and fragments thereof, having pesticidal activity against nematodes. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, biopesticide compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant parasitic nematodes.
摘要翻译：本发明提供具有针对线虫杀虫活性的新型多肽及其变体和片段。本发明的具体实施方案提供编码杀虫蛋白，生物农药组合物，表达盒，以及包含本发明的核酸的转化的微生物和植物的分离的核酸。这些组合物用于防治害虫，特别是植物寄生线虫的方法。

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