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1. 发明授权

US5324659A Yeast mutants useful for indentifying immunosupressants 失效
标题翻译：酵母突变体可用于鉴定免疫抑制剂
公开(公告)号：US5324659A
公开(公告)日：1994-06-28
申请号：US923082
申请日：1992-07-31
申请人： Stephen A. Parent , Leonardo E. Brizuela , Gary L. Chrebet , Keith A. Bostian
发明人： Stephen A. Parent , Leonardo E. Brizuela , Gary L. Chrebet , Keith A. Bostian
IPC分类号： C12N1/16 , C12N15/01 , C12Q1/02 , C12R1/865 , G01N33/50
CPC分类号： C12R1/85 , C12N15/01 , G01N33/5008 , Y10S435/942
摘要： Disclosed is a process utilizing yeast mutants for identifying active FK-506 type immunosuppressants. The process utilizes Saccharomyces cerevisiae mutants containing an fkr1, fkr2, fkr3 mutant gene or mixture thereof, which are resistant to FK-506 but sensitive to rapamycin. These mutants can be used in a diagnostic procedure for identifying FK-506 and FK-506 type immunosuppressants and in screening assays for compounds and fermentation broths which exhibit FK-506 type immunosuppressive activity. Specifically disclosed are the new yeast mutants, Saccharomyces cerevisiae YKF093, (Merck Culture Collection No. MY 2088) ATCC No. 74055, containing an fkr3 mutant gene, Saccharomyces cerevisiae YKF 012, (Merck Culture Collection No. MY 2096) ATCC No. 74061, which contains an fkr1 mutant gene, Saccharomyces cerevisiae YFK 014, (Merck Culture Collection No. MY 2097) ATCC No. 74062 and YFK-023-17A (Merck Culture Collection No. MY 2098) ATCC No. 74063, both which contain an fkr2 mutant gene and are useful in identifying active FK-506 type immunosuppressants.
摘要翻译：公开了利用酵母突变体鉴定活性FK-506型免疫抑制剂的方法。该方法利用含有fKr1，fkr2，fkr3突变基因或其混合物的酿酒酵母突变体，其对FK-506具有抗性，但对雷帕霉素敏感。这些突变体可用于鉴定FK-506和FK-506型免疫抑制剂的诊断程序，并用于展示FK-506型免疫抑制活性的化合物和发酵肉汤的筛选试验。具体公开的是含有fkr3突变基因，酿酒酵母YKF 012（Merck Culture Collection No. MY 2096）ATCC No.74061的新酵母突变体，酿酒酵母YKF093（Merck Culture Collection No. MY 2088）ATCC No.74055 ，其含有fkr1突变基因，酿酒酵母YFK 014（Merck Culture Collection No. MY 2097）ATCC No.74062和YFK-023-17A（Merck Culture Collection No. MY 2098）ATCC No.74063，它们都含有 fkr2突变基因，可用于鉴定活性FK-506型免疫抑制剂。

2. 发明授权

US5821353A DNA encoding 1,3 beta-D glucan synthase subunits 失效
标题翻译：编码1,3β-D葡聚糖合酶亚基的DNA
公开(公告)号：US5821353A
公开(公告)日：1998-10-13
申请号：US619554
申请日：1996-08-01
申请人： Cameron M. Douglas , Gary L. Chrebet , Joseph Clemas , Mohammed El-Sherbeini , Forrest Foor , Jennifer Nielsen Kahn , Rosemarie Kelly , Jean A. Marrinan , Nancy R. Morin , Janet C. Onishi , Stephen Authur Parent , Naasa M. Ramadan , Elizabeth A. Register , Gan-Ju Shei
发明人： Cameron M. Douglas , Gary L. Chrebet , Joseph Clemas , Mohammed El-Sherbeini , Forrest Foor , Jennifer Nielsen Kahn , Rosemarie Kelly , Jean A. Marrinan , Nancy R. Morin , Janet C. Onishi , Stephen Authur Parent , Naasa M. Ramadan , Elizabeth A. Register , Gan-Ju Shei
IPC分类号： G01N33/53 , A61K38/00 , A61K38/43 , A61P31/04 , C07H21/04 , C07K14/395 , C07K16/14 , C07K16/40 , C12N1/15 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/10 , C12N15/09 , C12P1/04 , C12R1/865 , C12P21/02
CPC分类号： C12N9/1051 , C07K14/395 , A61K38/00
摘要： DNA molecules encoding proteins involved in biosynthesis of 1,3-beta-D glucan are identified, cloned, expressed and used in in vitro assays to screen for antifungal compounds, including compounds that affect cell wall biosynthesis. The invention includes but is not limited to the purified DNA molecules, assays employing the DNA molecules, proteins encoded by the DNA molecules, cells expressing the DNA molecules and altered forms of the molecules.
摘要翻译： PCT No.PCT / US94 / 11498 Sec。 371日期：1996年8月1日 102（e）日期1996年8月1日PCT 1994年10月11日PCT PCT。 WO95 / 10625 PCT出版物 1995年4月20日，编码参与1,3-β-D葡聚糖生物合成的蛋白质的DNA分子被鉴定，克隆，表达并用于体外测定以筛选抗真菌化合物，包括影响细胞壁生物合成的化合物。本发明包括但不限于纯化的DNA分子，使用DNA分子的测定，由DNA分子编码的蛋白质，表达DNA分子的细胞和分子的改变形式。

3. 发明授权

US5587322A Replica plating device 失效
标题翻译：复制电镀装置
公开(公告)号：US5587322A
公开(公告)日：1996-12-24
申请号：US344217
申请日：1994-11-23
申请人： Gary L. Chrebet
发明人： Gary L. Chrebet
IPC分类号： C12M1/26 , C12M1/32
CPC分类号： C12M33/14 , C12M23/04 , C12M23/20
摘要： A device for replica plating of cellular colonies. The device has a solid body with a first region and a first end coupled to a peripheral ridge. A ridge is disposed at the transition from the first region to the peripheral ridge. A sheet of material capable of transferring cellular colonies from one medium to another is placed over the first end and the first region. A locking element defining an aperture passes over the first region and the sheet of material creating a frictional fit which pulls the sheet of material taut over the first end. The sheet of material is compressed between the locking element and the peripheral ridge thereby holding the sheet of material in place.
摘要翻译：用于细胞集落复制电镀的装置。该装置具有固体，其具有第一区域，第一端部联接到外围脊部。在从第一区域到外围脊的过渡处设置脊。能够将细胞集落从一种培养基转移到另一种培养基的材料片放置在第一端和第一区上。限定孔径的锁定元件穿过第一区域，并且该材料片材产生摩擦配合，其将片材拉伸在第一端部上。该片材料在锁定元件和周边脊之间被压缩，从而将材料片保持在适当位置。

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