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1. 发明授权

US08530415B2 Repair and/or regeneration of cells with a composition produced by culturing fibroblast cells under hypoxic conditions 有权
标题翻译：通过在缺氧条件下培养成纤维细胞产生的组合物修复和/或再生细胞
公开(公告)号：US08530415B2
公开(公告)日：2013-09-10
申请号：US12509171
申请日：2009-07-24
申请人： Gail K. Naughton , Frank Ziegler , Mark Baumgartner , Kyle Nickey
发明人： Gail K. Naughton , Frank Ziegler , Mark Baumgartner , Kyle Nickey
IPC分类号： C12P21/04 , C12N5/02 , A61K38/18
CPC分类号： A61K35/28 , A61K8/64 , A61K8/982 , A61K38/1709 , A61L27/3895 , A61L27/54 , A61L2300/252 , A61L2300/414 , A61L2300/45 , A61Q7/00 , A61Q19/00 , A61Q19/06 , A61Q19/08 , C07K14/47 , C12N5/0068 , C12N5/0656 , C12N2500/02
摘要： The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible three-dimensional surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.
摘要翻译：本发明涉及一种生产包含胚胎蛋白质的组合物的方法。该方法包括在体外在生物相容的三维表面上在缺氧条件下培养细胞。培养方法产生可溶性和不可溶性部分，其可以单独使用或组合使用以获得可用于各种医学和治疗应用的生理上可接受的组合物。

2. 发明授权

US08524494B2 Low oxygen tension and BFGF generates a multipotent stem cell from a fibroblast in vitro 有权
标题翻译：低氧压力和BFGF在体外从成纤维细胞产生多能干细胞
公开(公告)号：US08524494B2
公开(公告)日：2013-09-03
申请号：US12547422
申请日：2009-08-25
申请人： Gail K. Naughton , Frank Ziegler , Mark Baumgartner , Kyle Nickey
发明人： Gail K. Naughton , Frank Ziegler , Mark Baumgartner , Kyle Nickey
IPC分类号： C12N15/00 , C12N5/00
CPC分类号： C12N5/0075 , A61K8/981 , A61K35/33 , A61K38/00 , A61L27/227 , A61L27/3834 , A61L27/3895 , A61Q19/08 , C07K14/47 , C12N5/0068 , C12N5/0627 , C12N5/0656 , C12N5/0662 , C12N2500/02 , C12N2500/90 , C12N2533/90
摘要： The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.
摘要翻译：本发明涉及一种生产包含胚胎蛋白质的组合物的方法。该方法包括在体外在生物相容性表面的低氧条件下培养细胞。培养方法产生可溶性和不可溶性部分，其可以单独使用或组合使用以获得可用于各种医学和治疗应用的生理上可接受的组合物。

3. 发明申请

US20120230940A1 CONDITIONED CELL CULTURE MEDIUM COMPOSITIONS AND METHODS OF USE 有权
标题翻译：调节细胞培养基组合物和使用方法
公开(公告)号：US20120230940A1
公开(公告)日：2012-09-13
申请号：US13417017
申请日：2012-03-09
申请人： Gail K. Naughton , David L. Horwitz , Mark A. Applegate , Joan Zeltinger , Jonathan N. Mansbridge , Andreas Kern , Lee K. Landeen , Anthony Ratcliffe , R. Emmett Pinney
发明人： Gail K. Naughton , David L. Horwitz , Mark A. Applegate , Joan Zeltinger , Jonathan N. Mansbridge , Andreas Kern , Lee K. Landeen , Anthony Ratcliffe , R. Emmett Pinney
IPC分类号： A61K35/12 , A61K38/18 , A61K31/506 , C12N5/073
CPC分类号： A23L33/10 , A23V2002/00 , A61K8/02 , A61K8/0208 , A61K8/64 , A61K8/65 , A61K8/985 , A61K9/0014 , A61K35/33 , A61K35/35 , A61K35/36 , A61K35/50 , A61K38/18 , A61K38/1825 , A61K38/39 , A61K45/06 , A61K2800/86 , A61K2800/91 , A61L31/044 , A61L31/047 , A61L31/16 , A61L2300/414 , A61L2430/36 , A61Q7/00 , A61Q19/00 , A61Q19/08 , C12N5/0603 , C12N5/0606 , C12N5/0625 , C12N5/0629 , C12N5/0656 , C12N5/069 , C12N2502/1323 , C12P21/00 , A61K2300/00
摘要： Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, or formulated with a salve or ointment for topical applications. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.
摘要翻译：描述了包含调节细胞培养基组合物和使用方法的新产品。本发明的条件细胞培养基组合物可以由任何已知的定义或未定义的培养基组成，并且可以使用任何真核细胞类型进行调节。一旦本发明的细胞培养基被调节，它可以在任何状态下使用。条件培养基的物理实施方案包括但不限于液体或固体，冷冻，冻干或干燥成粉末。另外，介质配制有药学上可接受的载体作为用于内部施用的载体，直接施用于食品或产品，或用药膏或软膏配制用于局部应用。此外，可以进一步处理介质以浓缩或减少介质中包含的一种或多种因素或组分。

4. 发明申请

US20090123503A1 Conditioned Cell Culture Medium Compositions and Methods of Use 有权
标题翻译：条件细胞培养基组合物和使用方法
公开(公告)号：US20090123503A1
公开(公告)日：2009-05-14
申请号：US12349451
申请日：2009-01-06
申请人： Gail K. Naughton , David L. Horwitz , Mark A. Applegate , Joan Zeltinger , Jonathan N. Mansbridge , Andreas Kern , Lee K. Landeen , Anthony Ratcliffe , R. Emmett Pinney
发明人： Gail K. Naughton , David L. Horwitz , Mark A. Applegate , Joan Zeltinger , Jonathan N. Mansbridge , Andreas Kern , Lee K. Landeen , Anthony Ratcliffe , R. Emmett Pinney
IPC分类号： A61K8/64 , A61K38/18 , C12N5/06 , A61P17/14 , A61K9/10
CPC分类号： A23L33/10 , A23V2002/00 , A61K8/02 , A61K8/0208 , A61K8/64 , A61K8/65 , A61K8/985 , A61K9/0014 , A61K35/33 , A61K35/35 , A61K35/36 , A61K35/50 , A61K38/18 , A61K38/1825 , A61K38/39 , A61K45/06 , A61K2800/86 , A61K2800/91 , A61L31/044 , A61L31/047 , A61L31/16 , A61L2300/414 , A61L2430/36 , A61Q7/00 , A61Q19/00 , A61Q19/08 , C12N5/0603 , C12N5/0606 , C12N5/0625 , C12N5/0629 , C12N5/0656 , C12N5/069 , C12N2502/1323 , C12P21/00 , A61K2300/00
摘要： Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. The medium may be conditioned by stromal cells, parenchymal cells, mesenchymal stem cells, liver reserve cells, neural stem cells, pancreatic stem cells and/or embryonic stem cells. Additionally, the cells may be genetically modified. A three-dimensional tissue construct is preferred. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, formulated with a salve or ointment for topical applications, or, for example, made into or added to surgical glue to accelerate healing of sutures following invasive procedures. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.
摘要翻译：描述了包含调节细胞培养基组合物和使用方法的新产品。本发明的条件细胞培养基组合物可以由任何已知的定义或未定义的培养基组成，并且可以使用任何真核细胞类型进行调节。介质可以由基质细胞，实质细胞，间充质干细胞，肝储备细胞，神经干细胞，胰干细胞和/或胚胎干细胞调节。另外，细胞可以被遗传修饰。优选三维组织构建体。一旦本发明的细胞培养基被调节，它可以在任何状态下使用。条件培养基的物理实施方案包括但不限于液体或固体，冷冻，冻干或干燥成粉末。另外，介质配制有药学上可接受的载体作为内部给药载体，直接施用于用药膏或软膏配制用于局部应用的食品或产品，或者例如制成或添加到手术胶中通过侵入性手术加快缝合线愈合。此外，可以进一步处理介质以浓缩或减少介质中包含的一种或多种因素或组分。

5. 发明授权

US5858721A Three-dimensional cell and tissue culture system 失效
公开(公告)号：US5858721A
公开(公告)日：1999-01-12
申请号：US978520
申请日：1997-11-25
申请人： Gail K. Naughton , Brian A. Naughton
发明人： Gail K. Naughton , Brian A. Naughton
IPC分类号： A61B10/00 , A61B10/02 , A61B19/00 , A61K35/12 , A61K35/28 , A61L27/38 , A61L27/60 , C12N5/00 , C12N5/071 , C12N5/077 , C12N5/0789 , C12P21/02 , C12N5/10
CPC分类号： A61L27/3604 , A61B90/00 , A61K35/28 , A61L27/3886 , A61L27/60 , C12N5/0698 , A61B2010/0258 , A61K2035/124 , A61K35/12 , C12N2500/34 , C12N2501/11 , C12N2501/33 , C12N2501/335 , C12N2501/39 , C12N2502/091 , C12N2502/094 , C12N2502/1323 , C12N2503/04 , C12N2510/00 , C12N2533/30 , C12N2533/40 , C12N2533/70 , Y10S623/915
摘要： The present invention relates to a three-dimensional cell culture system which can be used to culture a variety of different cells and tissues in vitro for prolonged periods of time. In accordance with the invention, cells derived from a desired tissue are inoculated and grown on a pre-established stromal support matrix. The stromal support matrix comprises stromal cells, such as fibroblasts actively growing on a three-dimensional matrix. Stromal cells may also include other cells found in loose connective tissue such as endothelial cells, macrophages/monocytes, adipocytes, pericytes, reticular cells found in bone marrow stroma, etc. The stromal matrix provides the support, growth factors, and regulatory factors necessary to sustain long-term active proliferation of cells in culture. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of adult tissues analogous to counterparts found in vivo.

6. 发明授权

US5842477A Method for repairing cartilage 失效
标题翻译：软骨修复方法
公开(公告)号：US5842477A
公开(公告)日：1998-12-01
申请号：US604284
申请日：1996-02-21
申请人： Gail K. Naughton , Jane Willoughby
发明人： Gail K. Naughton , Jane Willoughby
IPC分类号： A61L27/00 , A61F2/00 , A61F2/02 , A61F2/30 , A61L27/36 , A61L27/38 , A61B19/00 , A61F2/08
CPC分类号： A61L27/3834 , A61F2/30756 , A61L27/3604 , A61L27/3654 , A61L27/3687 , A61L27/3852 , A61F2002/30062 , A61F2002/30199 , A61F2002/30766 , A61F2210/0004 , A61F2230/0063 , A61L2430/06 , Y10S623/902
摘要： The present invention relates to methods of making and/or repairing cartilage in vivo comprising implanting into a patient, at a site of cartilage damage or loss, a biocompatible, non-living three-dimensional scaffold or framework structure in combination with periosteal/perichondrial tissue that can be used to hold the scaffold in place and provides a source of chondrocyte progenitor cells, chondrocytes and other stromal cells for attachment to the scaffold in vivo. In addition, a preparation of cells that can include chondrocytes, chondrocyte progenitor cells or other stromal cells is administered, either before, during or after implantation of the scaffold and/or the periosteal perichondrial tissue; the cells are administered directly into the site of the implant in vivo and promote the induction of factors that enhance chondrogenesis and the migration of chondrocytes, progenitor cells and other stromal cells from the adjacent in vivo environment into the scaffold for the production of new cartilage at the site of implantation.
摘要翻译：本发明涉及在体内制备和/或修复软骨的方法，其包括将植入患者，软骨损伤或损失的部位，与骨膜/软骨膜组织相结合的生物相容性，非活体三维支架或骨架结构其可用于将支架保持在适当位置，并提供软骨细胞祖细胞，软骨细胞和其他基质细胞的来源，用于在体内附着到支架上。此外，在植入植入支架和/或骨膜软骨膜组织之前，期间或之后施用可以包括软骨细胞，软骨细胞祖细胞或其他基质细胞的细胞的制备物; 细胞在体内直接施用于植入物的位点，并且促进促进软骨形成和软骨细胞，祖细胞和其他基质细胞从相邻体内环境迁移到用于生产新软骨的支架中的因子的诱导植入部位。

7. 发明授权

US5510254A Three dimensional cell and tissue culture system 失效
标题翻译：三维细胞和组织培养系统
公开(公告)号：US5510254A
公开(公告)日：1996-04-23
申请号：US241259
申请日：1994-05-11
申请人： Brian A. Naughton , Gail K. Naughton
发明人： Brian A. Naughton , Gail K. Naughton
IPC分类号： A61B10/00 , A61B10/02 , A61B19/00 , A61K35/12 , A61K35/28 , A61L27/38 , A61L27/60 , C12N5/00 , C12N5/071 , C12N5/077 , C12N5/0789 , C12M3/00
CPC分类号： C12N5/0671 , A61B90/00 , A61K35/28 , A61L27/3604 , A61L27/3629 , A61L27/3637 , A61L27/3641 , A61L27/3804 , A61L27/3839 , A61L27/3886 , A61L27/3895 , A61L27/60 , A61B2010/0258 , A61K2035/124 , A61K35/12 , C12N2500/34 , C12N2501/11 , C12N2501/33 , C12N2501/335 , C12N2501/39 , C12N2502/13 , C12N2503/04 , C12N2510/00 , C12N2533/30 , C12N2533/40 , C12N2533/70
摘要： The present invention relates to a three-dimensional cell and tissue culture system. In particular, it relates to this culture system for the long term culture of liver cells and tissues in vitro in an environment that more closely approximates that found in vivo. The culture system described herein provides for proliferation and appropriate liver cell maturation to form structures analogous to tissue counterparts in vivo. The resulting liver tissues survive for prolonged periods, perform liver-specific functions, and maintain hepatic tissue architecture following in vivo implantation. The liver cultures have a variety of applications ranging from transplantation or implantation in vivo, to screening cytotoxic compounds and pharmaceutical compounds in vitro, to the production of biologically active molecules in "bioreactors" and to the construction of extracorporeal liver assist device.
摘要翻译：本发明涉及三维细胞和组织培养系统。特别地，它涉及这种用于在与体内发现更接近的环境中长期培养肝细胞和组织的培养体系。本文描述的培养系统提供增殖和合适的肝细胞成熟以形成类似于体内组织对应物的结构。所得肝组织长时间存活，进行肝特异性功能，并在体内植入后维持肝组织结构。肝脏培养物具有多种应用，从体内移植或植入到体外筛选细胞毒性化合物和药物化合物，在“生物反应器”中生产生物活性分子以及体外肝辅助装置的构建。

8. 发明授权

US5443950A Three-dimensional cell and tissue culture system 失效
标题翻译：三维细胞和组织培养系统
公开(公告)号：US5443950A
公开(公告)日：1995-08-22
申请号：US131361
申请日：1993-10-04
申请人： Gail K. Naughton , Brian A. Naughton
发明人： Gail K. Naughton , Brian A. Naughton
IPC分类号： A61B10/00 , A61B10/02 , A61B19/00 , A61K35/12 , A61K35/28 , A61L27/38 , A61L27/60 , C12N5/00 , C12N5/071 , C12N5/077 , C12N5/0789 , C12N5/08 , C12N5/22 , C12N11/14 , C12N11/00
CPC分类号： A61L27/3604 , A61B90/00 , A61K35/28 , A61L27/3886 , A61L27/60 , C12N5/0698 , A61B2010/0258 , A61K2035/124 , A61K35/12 , C12N2500/34 , C12N2501/11 , C12N2501/33 , C12N2501/335 , C12N2501/39 , C12N2502/091 , C12N2502/094 , C12N2502/1323 , C12N2503/04 , C12N2510/00 , C12N2533/30 , C12N2533/40 , C12N2533/70 , Y10S623/915
摘要： The present invention relates to a three-dimensional cell culture system which can be used to culture a variety of different cells and tissues in vitro for prolonged periods of time. In accordance with the invention, cells derived from a desired tissue are inoculated and grown on a pre-established stromal support matrix. The stromal support matrix comprises stromal cells, such as fibroblasts actively growing on a three-dimensional matrix. Stromal cells may also include other cells found in loose connective tissue such as endothelial cells, macrophages/monocytes, adipocytes, pericytes, reticular cells found in bone marrow stroma, etc. The stromal matrix provides the support, growth factors, and regulatory factors necessary to sustain long-term active proliferation of cells in culture. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of adult tissues analogous to counterparts found in vivo.
摘要翻译：本发明涉及三维细胞培养系统，其可用于在体外长时间培养各种不同的细胞和组织。根据本发明，将来自所需组织的细胞接种并在预先建立的基质支持基质上生长。基质支持基质包括基质细胞，例如在三维基质上积极生长的成纤维细胞。基质细胞还可以包括在松散结缔组织中发现的其他细胞，例如内皮细胞，巨噬细胞/单核细胞，脂肪细胞，周细胞，骨髓基质中发现的网状细胞等。基质基质提供了支持，生长因子和调节因子维持文化中细胞的长期积极扩散。当在该三维体系中生长时，增殖细胞成熟并适当地分离以形成类似于在体内发现的对应物的成体组织的组分。

9. 发明授权

US4721096A Process for replicating bone marrow in vitro and using the same 失效
标题翻译：体外复制骨髓的方法及其使用方法
公开(公告)号：US4721096A
公开(公告)日：1988-01-26
申请号：US36154
申请日：1987-04-03
申请人： Brian A. Naughton , Gail K. Naughton
发明人： Brian A. Naughton , Gail K. Naughton
IPC分类号： A61K35/12 , A61K35/28 , A61L27/60 , C12N5/077 , C12N5/0789 , A61B19/00 , C12N5/02
CPC分类号： C12N5/0669 , A61K35/28 , A61L27/60 , A61K2035/124 , C12N2500/34 , C12N2501/11 , C12N2501/33 , C12N2501/335 , C12N2501/39 , C12N2502/1323 , C12N2510/00 , C12N2533/30 , C12N2533/40 , C12N2533/70
摘要： According to the present invention there is provided a process for treating a person whose bone marrow has been destroyed or lost its functional ability. The process includes the steps of obtaining bone marrow from a donor, cryropreserving the marrow, replicating the bone marrow cells in vitro, and then infusing the replicated bone marrow cells into a person whose bone marrow has been destroyed or functionally compromised by disease or the treatment of disease. The person receiving the replicated bone marrow infusion may be the donor or another person. There is also disclosed a process for replicating bone marrow in vitro.
摘要翻译：根据本发明，提供了一种治疗骨髓已被破坏或失去其功能能力的人的方法。该方法包括以下步骤：从供体获得骨髓，冷冻保存骨髓，体外复制骨髓细胞，然后将复制的骨髓细胞注入骨髓已经被疾病或治疗破坏或功能受损的人的疾病接受复制骨髓输注的人可以是供体或另一个人。还公开了在体外复制骨髓的方法。

10. 发明授权

US08535913B2 Soluble composition for promoting hair growth produced by hypoxic culture of fibroblasts cells 有权
标题翻译：用于促进成纤维细胞的缺氧培养产生的毛发生长的可溶性组合物
公开(公告)号：US08535913B2
公开(公告)日：2013-09-17
申请号：US12632721
申请日：2009-12-07
申请人： Gail K. Naughton , Frank Ziegler , Mark Baumgartner , Kyle Nickey
发明人： Gail K. Naughton , Frank Ziegler , Mark Baumgartner , Kyle Nickey
IPC分类号： C12P21/04 , C12N15/02
CPC分类号： C07K14/47 , A61K8/982 , A61K38/00 , A61L27/34 , A61L27/3834 , A61L27/3895 , A61L29/085 , A61L31/10 , A61Q7/00 , A61Q19/08 , C12N5/0068 , C12N5/0627 , C12N5/069 , C12N2500/02 , C12N2500/90 , C12N2501/415 , C12N2533/90
摘要： The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible three-dimensional surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.
摘要翻译：本发明涉及一种生产包含胚胎蛋白质的组合物的方法。该方法包括在体外在生物相容的三维表面上在缺氧条件下培养细胞。培养方法产生可溶性和不可溶性部分，其可以单独使用或组合使用以获得可用于各种医学和治疗应用的生理上可接受的组合物。

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