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1. 发明授权

US10273470B2 Method for isolating RNA from a RNA and DNA containing sample 有权
公开(公告)号：US10273470B2
公开(公告)日：2019-04-30
申请号：US13580470
申请日：2011-02-25
申请人： Vera Holländer , Gabriele Christoffel
发明人： Vera Holländer , Gabriele Christoffel
IPC分类号： C12N15/00 , C12N15/10
摘要： The present invention pertains to a method for isolating RNA, including small RNA from a RNA and DNA containing sample, wherein the sample is lysed and the optionally further processed lysate is incubated with a DNase to degrade DNA prior to purifying the RNA from the optionally further processed lysate. It was found that performing the DNase digest prior to isolating the RNA from the lysate has considerable advantages.

2. 发明授权

US08573518B2 Method and device for breaking down biological material 有权
标题翻译：分解生物材料的方法和装置
公开(公告)号：US08573518B2
公开(公告)日：2013-11-05
申请号：US12989569
申请日：2009-04-24
申请人： Thomas Rothmann , Claudia Schmid , Andreas Schafer , Gabriele Christoffel
发明人： Thomas Rothmann , Claudia Schmid , Andreas Schafer , Gabriele Christoffel
IPC分类号： B02C19/00 , B02C11/00
CPC分类号： C12N1/066 , C12N13/00
摘要： The invention relates to a method for breaking down biological material, especially for obtaining biomolecules by ultrasound, the biological material being arranged, in a container together with a liquid.
摘要翻译：本发明涉及一种用于分解生物材料的方法，特别是通过超声波将生物分子与液体一起排列在容器中。

3. 发明申请

US20130225801A1 METHOD OF ISOLATING PURIFIED RNA WITH REDUCED DNA CONTAMINATIONS 有权
标题翻译：用减少的DNA污染物分离纯化的RNA的方法
公开(公告)号：US20130225801A1
公开(公告)日：2013-08-29
申请号：US13821159
申请日：2011-09-06
申请人： Gabriele Christoffel
发明人： Gabriele Christoffel
IPC分类号： C07H1/08
CPC分类号： C07H1/08 , C07H1/06 , C07H19/04 , C07H19/06 , C07H19/067 , C07H19/073 , C07H19/16 , C07H19/167 , C12N15/1003
摘要： The present invention pertains to a method of isolating RNA from a sample comprising RNA, and DNA, comprising: a) adding an acidic denaturing composition comprising a chaotropic agent and phenol to the sample; b) adding a water-insoluble organic solvent and separating the resulting phases thereby forming a multi-phase mixture comprising an aqueous phase, optionally an interphase and an organic phase, wherein the RNA is concentrated in said aqueous phase and DNA and proteins are concentrated in said organic phase and/or in said interphase; and c) isolating said RNA from said aqueous phase, wherein at least one cationic detergent is added before separating the phases. It was found that the addition of at least one cationic detergent considerably reduces the amount of DNA in the aqueous, RNA containing phase. Therefore, the present invention allows to easily isolate pure RNA which comprises considerably less DNA contaminations.
摘要翻译：本发明涉及从包含RNA和DNA的样品中分离RNA的方法，其包括：a）向样品中加入包含离液剂和苯酚的酸性变性组合物; b）加入水不溶性有机溶剂并分离所得的相，从而形成包含水相（任选地相间相和有机相）的多相混合物，其中RNA浓缩在所述水相中，并将DNA和蛋白质浓缩所述有机相和/或所述相间; 和c）从所述水相中分离所述RNA，其中在分离相之前加入至少一种阳离子去污剂。发现添加至少一种阳离子洗涤剂显着地减少含水RNA的相中的DNA的量。因此，本发明允许容易地分离包含相当少的DNA污染物的纯RNA。

4. 发明申请

US20130052721A1 METHOD FOR ISOLATING RNA FROM A RNA AND DNA CONTAINING SAMPLE 审中-公开
标题翻译：用于从RNA和包含样品的DNA中分离RNA的方法
公开(公告)号：US20130052721A1
公开(公告)日：2013-02-28
申请号：US13580470
申请日：2011-02-25
申请人： Vera Holländer , Gabriele Christoffel
发明人： Vera Holländer , Gabriele Christoffel
IPC分类号： C12N15/10 , C12S3/20
CPC分类号： C12N15/1003
摘要： The present invention pertains to a method for isolating RNA, including small RNA from a RNA and DNA containing sample, wherein the sample is lysed and the optionally further processed lysate is incubated with a DNase to degrade DNA prior to purifying the RNA from the optionally further processed lysate. It was found that performing the DNase digest prior to isolating the RNA from the lysate has considerable advantages.
摘要翻译：本发明涉及一种从RNA和含有DNA的样品中分离RNA的方法，其中将样品裂解，并将任选进一步处理的裂解物与DNA酶一起温育以降解DNA，然后从任选进一步纯化RNA 处理的裂解物。已经发现，在从裂解物分离RNA之前进行DNA酶消化物具有相当大的优点。

5. 发明授权

US08563262B2 Permanent inactivation of nucleases 有权
标题翻译：核酸酶永久失活
公开(公告)号：US08563262B2
公开(公告)日：2013-10-22
申请号：US12680515
申请日：2008-09-26
申请人： Gabriele Christoffel , Martin Schlumpberger , Heike Glowatz
发明人： Gabriele Christoffel , Martin Schlumpberger , Heike Glowatz
IPC分类号： C12Q1/44
CPC分类号： C12N15/1003
摘要： The present invention relates to the use of reagent for permanently inactivating nucleases wherein the reagent comprises an oxidizing agent, a protein denaturant and optionally a pH adjustor and to a method for permanently inactivating nucleases using said reagent.
摘要翻译：本发明涉及用于永久灭活核酸酶的试剂的用途，其中试剂包含氧化剂，蛋白质变性剂和任选的pH调节剂，以及使用所述试剂永久灭活核酸酶的方法。

6. 发明申请

US20100209993A1 PERMANENT INACTIVATION OF NUCLEASES 有权
标题翻译：核子的永久灭活
公开(公告)号：US20100209993A1
公开(公告)日：2010-08-19
申请号：US12680515
申请日：2008-09-26
申请人： Gabriele Christoffel , Martin Schlumpberger , Heike Glowatz
发明人： Gabriele Christoffel , Martin Schlumpberger , Heike Glowatz
IPC分类号： C12N9/99
CPC分类号： C12N15/1003
摘要： The present invention relates to the use of reagent for permanently inactivating nucleases wherein the reagent comprises an oxidizing agent, a protein denaturant and optionally a pH adjustor and to a method for permanently inactivating nucleases using said reagent.
摘要翻译：本发明涉及用于永久灭活核酸酶的试剂的用途，其中试剂包含氧化剂，蛋白质变性剂和任选的pH调节剂，以及使用所述试剂永久灭活核酸酶的方法。

7. 发明申请

US20140158619A1 FILTERING DEVICES COMPRISING CLAY MINERALS 审中-公开
标题翻译：包含粘土矿物的过滤装置
公开(公告)号：US20140158619A1
公开(公告)日：2014-06-12
申请号：US14233524
申请日：2012-07-20
申请人： Gabriele Christoffel
发明人： Gabriele Christoffel
IPC分类号： B01D39/20 , B01D69/14 , B01D15/08
CPC分类号： B01D39/2072 , B01D15/08 , B01D39/20 , B01D69/147
摘要： The present invention relates to a filtering device comprising clay minerals for the filtration of aqueous solutions used in biochemical and molecular biological applications. In particular, the present invention relates to the removal of undesired proteins from aqueous solutions by filtration through a filter comprising clay minerals.
摘要翻译：本发明涉及一种过滤装置，其包括用于过滤用于生物化学和分子生物学应用的水溶液的粘土矿物。特别地，本发明涉及通过包含粘土矿物质的过滤器过滤从水溶液中除去不想要的蛋白质。

8. 发明授权

US11542494B2 Method for isolating a target nucleic acid including small target nucleic acids with high yield 有权
公开(公告)号：US11542494B2
公开(公告)日：2023-01-03
申请号：US13820228
申请日：2011-09-02
申请人： Vera Holländer , Gabriele Christoffel , Martin Schlumpberger
发明人： Vera Holländer , Gabriele Christoffel , Martin Schlumpberger
IPC分类号： C12Q1/68 , C12N15/10 , C07H21/04
摘要： The present invention pertains to a method for isolating a target nucleic acid including small target nucleic acids from a sample, said method comprising at least the following steps a) binding at least a portion of the target nucleic acid including small target nucleic acids to a nucleic acid binding solid phase comprised in a column by passing the sample through said column, b) performing an enzymatic and/or chemical treatment on the nucleic acid binding solid phase while the target nucleic acid is bound to said solid phase, c) collecting at least a portion of the small target nucleic acids released from the solid phase during said treatment of step b) as flow-through, d) contacting said flow-through which comprises small target nucleic acids mixed with a recovery solution with a nucleic acid binding solid phase for binding the contained small target nucleic acids to said nucleic acid binding solid phase, e) optionally performing an elution. The present invention results in a considerable increase in the yield of small target nucleic acids in the isolated target nucleic acid because it allows to efficiently capture and recover small target nucleic acids.

9. 发明授权

US09487550B2 Method of isolating purified RNA with reduced DNA contaminations 有权
标题翻译：用减少的DNA污染物分离纯化的RNA的方法
公开(公告)号：US09487550B2
公开(公告)日：2016-11-08
申请号：US13821159
申请日：2011-09-06
申请人： Gabriele Christoffel
发明人： Gabriele Christoffel
IPC分类号： C07H21/00 , C07H1/08 , C12N15/10 , C07H19/06 , C07H19/16 , C07H19/073 , C07H19/167 , C07H1/06 , C07H19/067 , C07H19/04
CPC分类号： C07H1/08 , C07H1/06 , C07H19/04 , C07H19/06 , C07H19/067 , C07H19/073 , C07H19/16 , C07H19/167 , C12N15/1003
摘要： The present invention pertains to a method of isolating RNA from a sample comprising RNA, and DNA, comprising: a) adding an acidic denaturing composition comprising a chaotropic agent and phenol to the sample; b) adding a water-insoluble organic solvent and separating the resulting phases thereby forming a multi-phase mixture comprising an aqueous phase, optionally an interphase and an organic phase, wherein the RNA is concentrated in said aqueous phase and DNA and proteins are concentrated in said organic phase and/or in said interphase; and c) isolating said RNA from said aqueous phase, wherein at least one cationic detergent is added before separating the phases. It was found that the addition of at least one cationic detergent considerably reduces the amount of DNA in the aqueous, RNA containing phase. Therefore, the present invention allows to easily isolate pure RNA which comprises considerably less DNA contaminations.
摘要翻译：本发明涉及从包含RNA和DNA的样品中分离RNA的方法，其包括：a）向样品中加入包含离液剂和苯酚的酸性变性组合物; b）加入水不溶性有机溶剂并分离所得的相，从而形成包含水相（任选地相间相和有机相）的多相混合物，其中RNA浓缩在所述水相中，并将DNA和蛋白质浓缩所述有机相和/或所述相间; 和c）从所述水相中分离所述RNA，其中在分离相之前加入至少一种阳离子去污剂。发现添加至少一种阳离子洗涤剂显着地减少含水RNA的相中的DNA的量。因此，本发明允许容易地分离包含相当少的DNA污染物的纯RNA。

10. 发明申请

US20130164825A1 METHOD FOR ISOLATING A TARGET NUCLEIC ACID INCLUDING SMALL TARGET NUCLEIC ACIDS WITH HIGH YIELD 审中-公开
标题翻译：用于分离靶向核酸的方法，包括具有高YIELD的小目标核酸
公开(公告)号：US20130164825A1
公开(公告)日：2013-06-27
申请号：US13820228
申请日：2011-09-02
申请人： Vera Holländer , Gabriele Christoffel , Martin Schlumpberger
发明人： Vera Holländer , Gabriele Christoffel , Martin Schlumpberger
IPC分类号： C07H1/08
CPC分类号： C12N15/1006 , C12N15/1017
摘要： The present invention pertains to a method for isolating a target nucleic acid including small target nucleic acids from a sample, said method comprising at least the following steps a) binding at least a portion of the target nucleic acid including small target nucleic acids to a nucleic acid binding solid phase comprised in a column by passing the sample through said column, b) performing an enzymatic and/or chemical treatment on the nucleic acid binding solid phase while the target nucleic acid is bound to said solid phase, c) collecting at least a portion of the small target nucleic acids released from the solid phase during said treatment of step b) as flow-through, d) contacting said flow-through which comprises small target nucleic acids mixed with a recovery solution with a nucleic acid binding solid phase for binding the contained small target nucleic acids to said nucleic acid binding solid phase, e) optionally performing an elution. The present invention results in a considerable increase in the yield of small target nucleic acids in the isolated target nucleic acid because it allows to efficiently capture and recover small target nucleic acids.
摘要翻译：本发明涉及从样品中分离包含小靶核酸的靶核酸的方法，所述方法至少包括以下步骤：a）将包含小靶核酸的靶核酸的至少一部分结合至核酸通过使样品通过所述柱而包含在柱中的酸结合固相，b）对核酸结合固相进行酶和/或化学处理，同时靶核酸与所述固相结合，c）至少收集在步骤b）的所述处理期间从固相中释放的一部分小目标核酸作为流通，d）使所述流通接触，所述流过包含与回收溶液混合的小目标核酸与核酸结合固相用于将所含的小靶核酸与所述核酸结合固相结合，e）任选地进行洗脱。本发明导致分离的靶核酸中小靶核酸的产量显着增加，因为其允许有效捕获和回收小靶核酸。

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