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1. 发明申请

WO2011082293A1 METHODS FOR PRODUCING UNIQUELY SPECIFIC NUCLEIC ACID PROBES 审中-公开
标题翻译：用于生产独特的特异性核酸探针的方法
公开(公告)号：WO2011082293A1
公开(公告)日：2011-07-07
申请号：PCT/US2010/062485
申请日：2010-12-30
申请人： VENTANA MEDICAL SYSTEMS, INC. , ALEXANDER, Nelson , STANISLAW, Stacey , GRILLE, James , LEICK, Mark, B.
发明人： ALEXANDER, Nelson , STANISLAW, Stacey , GRILLE, James , LEICK, Mark, B.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6811 , C12Q1/6876
摘要： Disclosed herein are uniquely specific nucleic acid probes and methods for their use and production. The disclosed probes have reduced or eliminated background signal while reducing or eliminating the use of blocking DNA during hybridization. In one example, probes are produced by a method that includes joining at least a first binding region and a second binding region in a predetermined order and orientation, wherein the first binding region and second binding region are complementary to uniquely specific nucleic acid sequences, wherein the uniquely specific nucleic acid sequences are represented only once in a genome of an organism and wherein the first binding region and the second binding region include about 20% or less of a genomic target nucleic acid molecule. In particular examples, the binding regions ("uniquely specific binding regions") are complementary to non-contiguous portions of the genomic target nucleic acid. Methods of using the disclosed probes and kits including the probes and/or reagents for producing or using the probes are also disclosed.
摘要翻译：本文公开了独特的核酸探针及其使用和生产方法。所公开的探针具有减少或消除背景信号，同时在杂交期间减少或消除阻断DNA的使用。在一个实例中，通过包括以预定顺序和取向连接至少第一结合区和第二结合区的方法产生探针，其中第一结合区和第二结合区与独特的特异性核酸序列互补，其中独特的特异性核酸序列在生物体的基因组中仅表达一次，并且其中第一结合区和第二结合区包含基因组靶核酸分子的约20％或更少。在具体实例中，结合区（“唯一特异性结合区”）与基因组靶核酸的非连续部分互补。还公开了使用所公开的探针和试剂盒的方法，所述探针和试剂盒包括用于产生或使用探针的探针和/或试剂。

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