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    • 4. 发明申请
    • DETECTION OF AAD-1 EVENT DAS-40278-9
    • 检测AAD-1事件DAS-40278-9
    • WO2011022471A1
    • 2011-02-24
    • PCT/US2010/045871
    • 2010-08-18
    • DOW AGROSCIENCES LLCCUI, Yunxing, CoryGREENE, Thomas, WilliamNOVAK, StephenZHOU, Ning
    • CUI, Yunxing, CoryGREENE, Thomas, WilliamNOVAK, StephenZHOU, Ning
    • C12Q1/68C12P19/34
    • C12N15/8274C12N9/0069C12N9/0071
    • This invention relates in part to detecting herbicide tolerant plants - more specifically, an aad-1 transformation event in corn plants. The subject invention also provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). Kits and conditions useful in conducting the assays are also provided. The subject invention also relates in part to plant breeding using the subject methods. In some embodiments, said event / polynucleotide sequence can be "stacked" with other traits. More specifically, the invention relates in part to an endpoint TaqMan PCR assay for AAD-1 corn event 40278-9. Some embodiments are directed to assays that are capable of high throughput zygosity analysis. The subject invention further relates, in part, to the use of a preferred reference gene for use in determining zygosity.
    • 本发明部分地涉及检测除草剂耐受性植物 - 更具体地,涉及玉米植物中的aad-1转化事件。 本发明还提供了用于检测样品(例如玉米粒)中的受试者事件的存在的测定法。 还提供了用于进行测定的试剂盒和条件。 本发明也部分地涉及使用本发明方法的植物育种。 在一些实施方案中,所述事件/多核苷酸序列可以与其他特征“堆叠”。 更具体地,本发明部分涉及AAD-1玉米事件40278-9的终点TaqMan PCR测定。 一些实施方案涉及能够进行高通量接合度分析的测定。 本发明进一步涉及用于确定接合性的优选参考基因的用途。