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1. 发明申请

WO1995001443A1 MICROBIAL TRANSFORMANTS OF HANSENULA THAT EXPRESS GLYCOLATE OXIDASE AND CATALASE 审中-公开
标题翻译：明胶甘油酯氧化酶和催化剂的汉斯龙的微生物转化
公开(公告)号：WO1995001443A1
公开(公告)日：1995-01-12
申请号：PCT/US1994007080
申请日：1994-06-29
申请人： E.I. DU PONT DE NEMOURS AND COMPANY , ANTON, David, Leroy , DiCOSIMO, Robert , DAHLEMS, Ulrike, Magdalene , GELLISSEN, Gerd, Johann , JONOWICZ, Zbigniew, Alojzy , PIONTEK, Michael
发明人： E.I. DU PONT DE NEMOURS AND COMPANY
IPC分类号： C12N15/53
CPC分类号： C12N9/0065 , C12N9/0006 , C12N15/815
摘要： This invention relates to Hansenula strains that have been genetically engineered to produce glycolate oxidase, optionally to also produce catalase, which serve as biocatalysts in the conversion of glycolic acid to glyoxylic acid.
摘要翻译：本发明涉及经遗传工程改造以生产乙醇酸氧化酶的汉逊酵母菌株，任选地也产生过氧化氢酶，其用作将乙醇酸转化为乙醛酸的生物催化剂。

2. 发明公开

EP1698702A1 Recombinant protein expression system 有权
标题翻译：表达系统重组蛋白
公开(公告)号：EP1698702A1
公开(公告)日：2006-09-06
申请号：EP05004550.9
申请日：2005-03-02
申请人： Gellissen, Gerd, Prof. Dr.
发明人： Gellissen, Gerd, Prof. Dr.
IPC分类号： C12N15/81
CPC分类号： C12N15/81
摘要： The present invention relates to a polynucleotide comprising functionally linked a nucleic acid sequence comprising an autonomously replicating sequence element; a nucleic acid sequence comprising a ribosomal DNA (rDNA) gene; a nucleic acid sequence encoding a selection marker; and a nucleic acid sequence comprising a nucleic acid sequence encoding a expressible polypeptide and a promoter nucleic acid sequence governing the expression of said expressible polypeptide. Moreover, the invention encompasses vectors, host cells or kits comprising said polynucleotide or methods for the manufacture of a protein or a pharmaceutical composition using said polynucleotide. Finally, the invention relates to collections of polynucleotides which can be assembled and a kit comprising said collections of polynucleotides.
摘要翻译：本发明涉及包含功能性连接的包含自主复制序列元件的核酸序列的多核苷酸; 包含核糖体DNA（rDNA）基因的核酸序列; 编码选择标记的核酸序列; 以及包含编码可表达多肽的核酸序列和控制所述可表达多肽的表达的启动子核酸序列的核酸序列。此外，本发明包括使用所述多核苷酸的载体，宿主细胞或包含所述多核苷酸的试剂盒或用于制备蛋白质或药物组合物的方法。最后，本发明涉及可以组装的多核苷酸的集合和包含所述多核苷酸集合的试剂盒。

3. 发明授权

EP1177305B1 VERFAHREN ZUM HERSTELLEN EINES REKOMBINANTEN PROTEINS 有权
标题翻译：方法生产重组蛋白质的
公开(公告)号：EP1177305B1
公开(公告)日：2007-12-26
申请号：EP00931125.9
申请日：2000-05-05
申请人： RHEIN BIOTECH GESELLSCHAFT FÜR NEUE BIOTECHNOLOGISCHE PROZESSE UND PRODUKTE MBH , Gellissen, Gerd Prof. Dr.
发明人： FARWICK, Mike , LONDON, Markus , DOHMEN, Juergen , DAHLEMS, Ulrike , GELLISSEN, Gerd , STRASSER, Alexander, W.
IPC分类号： C12N15/67 , C07K14/47 , C12N9/12 , C12N1/15 , G01N33/50
CPC分类号： C07K14/395 , C12N15/79 , C12N15/81
摘要： The invention relates to a method for producing recombinant proteins, in particular, recombinant secretory proteins, and to a method for identifying nucleic acid molecules whose expression products enable an improved secretion of a recombinant secretory protein. The invention also relates to the use of molecules identified in this manner for reinforcing the secretion of heterologous proteins and to corresponding kit systems. The inventive method provides that a nucleic acid which codes for a secretory protein is expressed in a suitable host cell together with a nucleic acid that codes for a polypeptide with the biological activity of the eukaryontic translation-initiation factor 4 E and/or with a nucleic acid that codes for a polypeptide with the biological activity of a CaM kinase. The invention also provides that the coexpression of the translation-initiation factor gene and/or of the CaM kinase gene leads to an improved secretion of a desired recombinant protein out of the cell.

4. 发明授权

EP1151112B1 HITZEINDUZIERBARER PROMOTOR 有权
标题翻译：热诱导启动子
公开(公告)号：EP1151112B1
公开(公告)日：2010-01-20
申请号：EP00912457.9
申请日：2000-02-11
申请人： RHEIN BIOTECH GESELLSCHAFT FÜR NEUE BIOTECHNOLOGISCHE PROZESSE UND PRODUKTE MBH , Gellissen, Gerd, Prof. Dr.
发明人： ROMANO, Ivano , GELLISSEN, Gerd , DE VIRGILIO, Claudio
IPC分类号： C12N15/55 , C12N9/16
CPC分类号： C07K14/39

5. 发明专利

AT382089T 未知
公开(公告)号：AT382089T
公开(公告)日：2008-01-15
申请号：AT00931125
申请日：2000-05-05
申请人： RHEIN BIOTECH PROZ & PROD GMBH , GELLISSEN GERD PROF DR
发明人： FARWICK MIKE , LONDON MARKUS , DOHMEN JUERGEN , DAHLEMS ULRIKE , GELLISSEN GERD , STRASSER ALEXANDER
IPC分类号： C12N15/67 , C07K14/395 , C07K14/47 , C12N1/15 , C12N1/19 , C12N9/12 , C12N15/55 , C12N15/79 , C12N15/81 , G01N33/50
摘要： The present invention relates to methods for producing recombinant proteins, in particular recombinant secretory proteins, to a method for identifying nucleic acid molecules the expression products of which permit improved secretion of a recombinant secretory protein, to the use of molecules thus identified for enhancing secretion of heterologous proteins, and to corresponding kit systems. The method according to the invention makes provision for expression in a suitable host cell of a nucleic acid coding for a secretory protein together with a nucleic acid coding for a polypeptide having the biological activity of the eukaryotic translation initiation factor 4 E and/or for a polypeptide having the biological activity of a CaM kinase. It was found that coexpression of the translation initiation factor gene and/or of the CaM kinase gene leads to improved secretion of a desired recombinant protein from the cell.

6. 发明申请

WO02053758A2 METHOD FOR PRODUCING HETEROLOGOUS PROTEINS IN A HOMOTHALLIC FUNGUS OF THE SORDARIACEAE FAMILY 审中-公开
标题翻译：生产家蝇科的同源蘑菇中的异源蛋白质的方法
公开(公告)号：WO02053758A2
公开(公告)日：2002-07-11
申请号：PCT/EP0115354
申请日：2001-12-28
申请人： RHEIN BIOTECH PROZ & PROD GMBH , KUECK ULRICH , POEGGELER STEFANIE , MASLOFF SANDRA , NOWROUSIAN MINOU , BARTELSEN OLIVER , GELLISSEN GERD , PFEIL MICHAEL
发明人： KUECK ULRICH , POEGGELER STEFANIE , MASLOFF SANDRA , NOWROUSIAN MINOU , BARTELSEN OLIVER , GELLISSEN GERD , PFEIL MICHAEL
IPC分类号： C12N1/15 , C12N15/80 , C12P21/00
CPC分类号： C12R1/645 , C12N15/80 , C12P21/00
摘要： The invention relates to a method for producing a heterologous protein in a filamentous fungus and to suitable promoters therefor. The invention also relates to vectors and host cells and to a kit and the use thereof. The aim of the invention is to provide a method for producing a heterologous protein in a filamentous fungus, which allows the efficient production of heterologous proteins and in which a contamination of the created protein by vegetative spores is prevented. To achieve this, the invention discloses a method for producing a heterologous protein in a filamentous fungus, which comprises (a) the cultivation of a homothallic fungus of the Sordariaceae family, which contains an expression cassette with the following elements in a functional combination: a promoter that is active in the fungus of the Sordariaceae family; a heterologous gene and a terminator that is active in the fungus of the Sordariaceae family and (b) the harvesting of the created protein in a manner known per se.
摘要翻译：本发明涉及在丝状真菌中产生异源蛋白质的方法以及合适的启动子。此外，本发明涉及载体和宿主细胞以及试剂盒及其用途。本发明的目的是提供一种用于制造异源蛋白质的丝状真菌，其允许有效地生产异源蛋白质的和在生产由营养孢子避免蛋白质的其污染的方法。在的真菌一个：根据本发明，该目的通过一种用于在（a）培养的Sordariaceae家族包含Espressionskassette的同宗配合真菌是丝状真菌生产的异源蛋白质，含有有效连接的以下元件来实现 Sordariaceae活性启动子; 在Sordariaceae科的真菌中有活性的异源基因和终止子;和（b）以本身已知的方式收获产生的蛋白质。

7. 发明申请

WO0138510A3 INTEGRATION VECTORS AND METHOD FOR PRODUCING RECOMBINANT PROTEINS IN FUNGI 审中-公开
标题翻译：整合载体及其制造方法在真菌中的重组蛋白
公开(公告)号：WO0138510A3
公开(公告)日：2002-07-04
申请号：PCT/EP0011687
申请日：2000-11-23
申请人： RHEIN BIOTECH PROZ & PROD GMBH , GELLISSEN GERD , DIESEL ANDRE , KLABUNDE JENS , SUCKOW MANFRED , HOLLENBERG CORNELIS P
发明人： GELLISSEN GERD , DIESEL ANDRE , KLABUNDE JENS , SUCKOW MANFRED , HOLLENBERG CORNELIS P
IPC分类号： C07K14/62 , C12N9/16 , C12N15/80 , C12N15/81 , C12N1/15 , C12N1/19 , C12N15/67 , C12N15/90
CPC分类号： C07K14/62 , C12N9/16 , C12N15/80 , C12N15/815
摘要： The invention relates to integration vectors for the targeted and stable integration of heterologous DNA into the genome of a host organism, especially a fungus, and to the use thereof. The invention also relates to a method for producing one or more recombinant proteins using the inventive vectors. The aim of the invention is to provide integration vectors which enable foreign DNA to be integrated into the genome of a host organism in a stable manner and with a high copy number and if necessary, to enable different genes to be expressed for a simultaneous coproduction of different proteins. Another aim of the invention is provide stable production strains and a method for producing heterologous proteins. To this end, an integration vector comprising the following is provided: a) at least one rDNA sequence from yeast; b) at least one non-deficient selection marker gene; and c) at least one expression cassette, said expression cassette comprising a promoter element that is functional in the host organism, an area of a heterologous or genuine gene that code for a desired protein and a terminator element that is functional in the host organism. The invention also comprises a host organism containing at least one inventive vector. A preferred organism is the methylotrophic yeast Hansenula polymorpha .
摘要翻译：本发明涉及用于靶向整合和稳定整合的异源DNA的引入宿主生物体的基因组，特别是真菌，和它们的用途的载体。此外，本发明涉及一种用于制造使用本发明的载体的一种或多种重组蛋白。本发明的目的是提供一种整合载体，其使得有可能在高拷贝数外源DNA整合稳定地引入宿主生物体的基因组，并根据需要对表达同时共生产各种蛋白的不同基因。本发明的另一个目的是提供稳定的生产菌株和用于产生异源蛋白质的方法。这个目的是通过整合载体，其包括实现：a）至少一种rDNA的来自酵母的序列，b）至少一个非缺陷型选择标记基因和c）至少一个表达盒，所述表达盒在宿主生物体中的启动子元件的功能，一个赞成的蛋白质包含异源或真正基因的编码区和在宿主生物中终止子元件的功能。宿主生物体也包括在本发明中，含有至少一种本发明的载体。优选的宿主生物是甲基营养酵母多形汉逊。

8. 发明申请

WO2002053758A3 VERFAHREN ZUM HERSTELLEN VON HETEROLOGEN PROTEINEN IN EINEM HOMOTHALLISCHEN PILZ DER FAMILIE SORDARIACEAE 审中-公开
公开(公告)号：WO2002053758A3
公开(公告)日：2002-07-11
申请号：PCT/EP2001/015354
申请日：2001-12-28
申请人： RHEIN BIOTECH GESELLSCHAFT FÜR NEUE BIOTECHNOLOGISCHE PROZESSE UND PRODUKTE MBH , KÜCK, Ulrich , PÖGGELER, Stefanie , MASLOFF, Sandra , NOWROUSIAN, Minou , BARTELSEN, Oliver , GELLISSEN, Gerd , PFEIL, Michael
发明人： KÜCK, Ulrich , PÖGGELER, Stefanie , MASLOFF, Sandra , NOWROUSIAN, Minou , BARTELSEN, Oliver , GELLISSEN, Gerd , PFEIL, Michael
IPC分类号： C12N15/80
摘要： Die vorliegende Erfindung betrifft ein Verfahren zum Herstellen von heterologem Protein in einem filamentösen Pilz sowie dafür geeignete Promotoren. Ferner betrifft die vorliegende Erfindung Vektoren und Wirtszellen sowie einen Kit und deren Verwendung. Aufgabe der vorliegenden Erfindung ist es, ein Verfahren zum herstellen von heterologem Protein in einem filamentösen Pilz bereitzustellen, das eine effiziente Produktion heterologer Proteine erlaubt und bei dem eine Kontamination des erzeugten Proteins durch vegetative Sporen vermieden wird. Erfindungsgemäß wird diese Aufgabe durch ein Verfahren zum Herstellen von heterologem Protein in einem filamentösen Pilz gelöst, das (a) das Kultivieren eines homothallischen Pilzes der Familie Sordariaceae, der eine Espressionskassette enthält, die in funktioneller Verbindung die folgenden Elemente enthält: einen in dem Pilz der Familie Sordariaceae aktiven Promotor; ein heterologes Gen und einen in dem Pilz der Familie Sordariaceae aktiven Terminator, sowie (b) das Ernten des erzeugten Proteins in an sich bekannter Weise umfasst.

9. 发明申请

WO0040727A3 RECOMBINANT GROWTH FACTOR WITH THE BIOLOGICAL ACTIVITY OF A G-CSF (GRANULOCYTE COLONY STIMULATING FACTOR) 审中-公开
标题翻译：重组生长因子与G-CSF（粒细胞集落刺激因子）的生物活性
公开(公告)号：WO0040727A3
公开(公告)日：2000-10-26
申请号：PCT/EP9910466
申请日：1999-12-29
申请人： RHEIN BIOTECH PROZ & PROD GMBH , FISCHER JOHANNES , WERNET PETER , GELLISSEN GERD , JENZELEWSKI VOLKER , PIONTEK MICHAEL , STRASSER ALEXANDER W , WEYDEMANN ULRIKE
发明人： FISCHER JOHANNES , WERNET PETER , GELLISSEN GERD , JENZELEWSKI VOLKER , PIONTEK MICHAEL , STRASSER ALEXANDER W , WEYDEMANN ULRIKE
IPC分类号： C07K14/535 , C12N1/21 , C12N15/12 , C12N15/27 , A61K31/70 , A61K38/19 , A61K48/00 , C07K1/14 , C12N1/15 , C12N1/19 , C12N5/10 , C12N15/62
CPC分类号： C07K14/535
摘要： The invention relates to growth factors with granulocyte colony stimulating activity and to nucleic acid molecules which comprise a sequence coding for said growth factor. The invention also relates to a method for producing the inventive growth factor, to pharmaceutical compositions which comprise the inventive proteins or nucleic acids and to the therapeutical utilisation thereof. The growth factors have cytokine effects of different degrees and can stimulate the maturation of blood cells. The inventive molecules are particularly useful for treating diseases and wounds which are related to a blood cell deficiency such as cancer, leukemia, severe burns and opportunistic infections and after bone marrow transplantations.
摘要翻译：本发明提供了具有粒细胞集落刺激活性的生长因子以及包含编码该生长因子的序列的核酸分子。本发明还涉及用于产生根据本发明的生长因子的方法，涉及包含根据本发明的蛋白质或核酸的药物组合物以及它们的治疗用途。生长因子具有不同的细胞因子效应，并且能够刺激血细胞的成熟。因此设想使用本发明的分子，特别是用于治疗与血细胞缺乏相关的疾病和损伤，例如，癌症，白血病，严重烧伤，机会性感染和骨髓移植。

10. 发明专利

AT455856T 未知
公开(公告)号：AT455856T
公开(公告)日：2010-02-15
申请号：AT00912457
申请日：2000-02-11
申请人： RHEIN BIOTECH PROZ & PROD GMBH , GELLISSEN GERD PROF DR
发明人： ROMANO IVANO , GELLISSEN GERD , DE VIRGILIO CLAUDIO
IPC分类号： C12N15/09 , C12N15/55 , C07K14/39 , C12N1/19 , C12N1/21 , C12N9/16 , C12N15/63 , C12N15/81 , C12P21/00 , C12P21/02 , C12R1/78
摘要： The invention relates to nucleic acid molecules comprising a heat-inducible promoter and to expression vectors and host cells containing at least one nucleic acid molecule according to the invention. The present invention further relates to kits and methods for producing one or more proteins using the nucleic acid molecules according to the invention and to various uses of the same. The object of the invention is to provide a promoter the heat-inducible characteristic of which is as selective as possible, in particular a promoter which is active in yeasts and which is suitable for protein expression at high temperatures. This object is fulfilled by a nucleic acid molecule comprising a heat-inducible promoter of a Hansenula polymorpha gene coding for a protein with trehalose-6-phosphate-synthase activity;

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