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    • 5. 发明授权
    • Recombinant bateriophage for heterologous cloning of bacillus
microorganisms and method for its production
    • 用于异源克隆芽孢杆菌微生物的重组噬菌体及其生产方法
    • US4886754A
    • 1989-12-12
    • US740312
    • 1985-06-03
    • Richard S. GrahamYuko YonedaFrank E. Young
    • Richard S. GrahamYuko YonedaFrank E. Young
    • C12N7/01C12N9/28C12N15/75
    • C12N9/2417C12N15/75Y10S435/832Y10S435/839
    • A recombinant bacteriophage, a method for producing and selecting the recombinant bacteriophage and a method for heterologous cloning of DNA are disclosed. The recombinant bacteriophage is produced by ligating genetic fragments encoding a desired genetic trait with DNA from a bacteriophage, incubating with DNA from a second Bacillus microorganism prototrophic for a growth requirement, incubating with a host Bacillus auxotrophic for the growth requirement. Transformed host Bacillus are selected by growing the mixture on a growth medium which does not contain the growth requirement and determining the presence of the genetic trait. The recombinant bacteriophage containing the desired genetic trait is recovered from the host Bacillus by induction. Heterologous cloning can be accomplished by incubating a host Bacillus with the recombinant bacteriophage.
    • 公开了重组噬菌体,重组噬菌体的制备和选择方法以及异源克隆DNA的方法。 通过将编码期望的遗传性状的遗传片段与来自噬菌体的DNA连接起来,与来自第二种芽孢杆菌微生物原生质体的DNA一起孵育以用于生长需求,通过与宿主芽孢杆菌营养缺陷型孵育用于生长需要来产生重组噬菌体。 通过在不含生长需要并确定遗传性状的存在的生长培养基上生长混合物来选择转化的宿主芽孢杆菌。 通过诱导从宿主芽孢杆菌中回收含有所需遗传特征的重组噬菌体。 可以通过将宿主芽孢杆菌与重组噬菌体一起孵育来实现异源克隆。