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1. 发明授权

US08304245B2 Microfluidic flow lysometer device, system and method 有权
标题翻译：微流体流变仪器，系统及方法
公开(公告)号：US08304245B2
公开(公告)日：2012-11-06
申请号：US11934718
申请日：2007-11-02
申请人： Franciscus Albertus Kuypers , Won Chul Lee , Albert P. Pisano
发明人： Franciscus Albertus Kuypers , Won Chul Lee , Albert P. Pisano
IPC分类号： C12Q1/34 , C12Q1/68 , C12Q1/00 , C12M1/34 , C12M1/00
CPC分类号： G01N33/5094 , B01L3/5027 , G01N15/147 , G01N15/1475 , G01N2015/0069 , G01N2015/149 , G01N2015/1497
摘要： The invention provides a device, system and method that enables a microfluidic flow lysometer cell analyzer. Using a population of suspended living cells. cell surface molecule detection reagents, and cell cytoplasm (or nuclear) molecule detection reagents, this microfluidic cell analyzer can rapidly analyze a population of cells by running them on a one-at-a-time basis through small capillary channels. The cell's morphology or surface markers are analyzed, then the cells are lysed, and the molecules present in the cell's cytoplasm or nuclear material are analyzed. Cell morphology is then analyzed as the cell surface molecules are correlated with the molecules present in the same cell's cytoplasm or nucleic acids, and this correlated cell population data is then presented to a user for interpretation. The invention also addresses issues such as device fouling, correction for experimental artifacts (incomplete cell lyis, variable cell debris volume effects), and inadequate data collection that hampered earlier efforts in this area.
摘要翻译：本发明提供一种能够实现微流体流动溶血计细胞分析仪的装置，系统和方法。使用悬浮活细胞群。细胞表面分子检测试剂和细胞质（或核）分子检测试剂，这种微流体细胞分析仪可以通过一小时的毛细管通道一次运行来快速分析细胞群。分析细胞的形态或表面标志物，然后细胞裂解，分析存在于细胞的细胞质或核材料中的分子。然后分析细胞形态，因为细胞表面分子与存在于相同细胞的细胞质或核酸中的分子相关，然后将相关的细胞群体数据呈现给用户进行解释。本发明还涉及诸如装置结垢，实验工件校正（不完全细胞裂解，可变细胞碎片体积效应）等问题，以及阻碍早期在该领域的努力的数据收集不足。

2. 发明申请

US20090117555A1 Microfluidic Flow Lysometer Device, System and Method 有权
标题翻译：微流体流动测量仪器，系统和方法
公开(公告)号：US20090117555A1
公开(公告)日：2009-05-07
申请号：US11934718
申请日：2007-11-02
申请人： Franciscus Albertus Kuypers , Won Chul Lee , Albert P. Pisano
发明人： Franciscus Albertus Kuypers , Won Chul Lee , Albert P. Pisano
IPC分类号： C12Q1/04 , C12M1/34 , C12Q1/68
CPC分类号： G01N33/5094 , B01L3/5027 , G01N15/147 , G01N15/1475 , G01N2015/0069 , G01N2015/149 , G01N2015/1497
摘要： The invention provides a device, system and method that enables a microfluidic flow lysometer cell analyzer. Using a population of suspended living cells. cell surface molecule detection reagents, and cell cytoplasm (or nuclear) molecule detection reagents, this microfluidic cell analyzer can rapidly analyze a population of cells by running them on a one-at-a-time basis through small capillary channels. The cell's morphology or surface markers are analyzed, then the cells are lysed, and the molecules present in the cell's cytoplasm or nuclear material are analyzed. Cell morphology is then analyzed as the cell surface molecules are correlated with the molecules present in the same cell's cytoplasm or nucleic acids, and this correlated cell population data is then presented to a user for interpretation. The invention also addresses issues such as device fouling, correction for experimental artifacts (incomplete cell lyis, variable cell debris volume effects), and inadequate data collection that hampered earlier efforts in this area.
摘要翻译：本发明提供一种能够实现微流体流动溶血计细胞分析仪的装置，系统和方法。使用悬浮活细胞群。细胞表面分子检测试剂和细胞质（或核）分子检测试剂，这种微流体细胞分析仪可以通过一小时的毛细管通道一次运行来快速分析细胞群。分析细胞的形态或表面标志物，然后细胞裂解，分析存在于细胞的细胞质或核材料中的分子。然后分析细胞形态，因为细胞表面分子与存在于相同细胞的细胞质或核酸中的分子相关，然后将相关的细胞群体数据呈现给用户进行解释。本发明还涉及诸如装置结垢，实验工件校正（不完全细胞裂解，可变细胞碎片体积效应）等问题，以及阻碍早期在该领域的努力的数据收集不足。

3. 发明授权

US10247656B2 Single-cell microchamber array 有权
公开(公告)号：US10247656B2
公开(公告)日：2019-04-02
申请号：US13224064
申请日：2011-09-01
申请人： Franciscus Albertus Kuypers , Won Chul Lee , Albert P. Pisano
发明人： Franciscus Albertus Kuypers , Won Chul Lee , Albert P. Pisano
IPC分类号： C40B30/00 , G01N15/14 , G01N15/10 , G01N15/00
摘要： An apparatus for analyzing individual cell composition in a heterogeneous cell population may include, in one embodiment, a deposition plate having an array of microwells disposed therein, and a cover plate substantially overlying the deposition plate. A pair of electrodes may be associated with one or more of the microwells, and may be configured to generate an electric field within the associated microwell.

4. 发明申请

US20120065082A1 SINGLE-CELL MICROCHAMBER ARRAY 审中-公开
标题翻译：单细胞微阵列
公开(公告)号：US20120065082A1
公开(公告)日：2012-03-15
申请号：US13224064
申请日：2011-09-01
申请人： Franciscus Albertus Kuypers , Won Chul Lee , Albert P. Pisano
发明人： Franciscus Albertus Kuypers , Won Chul Lee , Albert P. Pisano
IPC分类号： C40B30/00 , C40B60/12
CPC分类号： G01N15/1484 , G01N15/1056 , G01N15/1475 , G01N2015/0069 , G01N2015/1486
摘要： An apparatus for analyzing individual cell composition in a heterogeneous cell population may include, in one embodiment, a deposition plate having an array of microwells disposed therein, and a cover plate substantially overlying the deposition plate. A pair of electrodes may be associated with one or more of the microwells, and may be configured to generate an electric field within the associated microwell.
摘要翻译：在一个实施例中，用于分析异种细胞群体中的个体细胞组成的装置可以包括其中布置有微孔阵列的沉积板和基本上覆盖沉积板的盖板。一对电极可以与一个或多个微孔相关联，并且可以被配置为在相关联的微孔内产生电场。

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