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1. 发明授权

US4629696A Process for the selective production of reduced oxygen species 失效
标题翻译：选择性生产还原氧物质的方法
公开(公告)号：US4629696A
公开(公告)日：1986-12-16
申请号：US537445
申请日：1983-09-30
申请人： Erich Elstner
发明人： Erich Elstner
IPC分类号： C12Q1/26 , C12Q1/28 , C12Q1/32
CPC分类号： C12Q1/28 , C12Q1/26 , Y10S435/81
摘要： Process for the selective production of the reduced oxygen species superoxide, hydrogen peroxide and hydroxyl radicals, wherein oxygen is reduced with NAD(P)H in the presence of an NAD(P)H-dependent, non-autoxidisable diaphorase, of an appropriate autoxidisable redox partner and optionally of an appropriate buffer system.Also, process for the determination of superoxide dismutase in which a superoxide-yielding reaction is coupled with two competing superoxide-consuming reactions, one superoxide-consuming reaction being a conventional indicator reaction and the other superoxide-consuming reaction being the superoxide dismutase reaction, wherein, as superoxide-yielding reaction, there is used the reduction of oxygen with NAD(P)H in the presence of an NAD(P)H-dependent, non-autoxidisable diaphorase, of an autoxidisable one-electron step-inducing redox partner with a one-electron redox potential in the range of from -150 mV to -500 mV and optionally of an appropriate buffer system.Also, process for the determination of NAD(P)H and of NAD(P)H-yielding reactions in which oxygen is reduced to hydrogen peroxide by NAD(P)H and the hydrogen peroxide is determined in knowm manner, wherein the reduction of the oxygen by NAD(P)H is carried out in the presence of an NAD(P)H-dependent, non-autoxidisable diaphorase, of an appropriate two-electron redox partner with a two-electron redox potential in the range of from 0 mV to 150 mV and of an appropriate buffer system.Also, reagents for carrying out these processes.
摘要翻译：选择性生产还原氧超氧化物，过氧化氢和羟基的方法，其中在NAD（P）H依赖性，不可自动氧化的心肌黄酶存在下，用NAD（P）H还原氧合物，其具有适当的可自动氧化氧化还原对象和可选的适当的缓冲系统。此外，超氧化物歧化酶的测定方法，其中超氧化物产生反应与两个相互竞争的超氧化物消耗反应相结合，一个超氧化物消耗反应是常规指示反应，另一个超氧化物消耗反应是超氧化物歧化酶反应，其中作为超氧化物产生反应，在NAD（P）H依赖性，不可自发氧化的心肌黄酶的存在下，使用NAD（P）H还原氧自由基的单电子步骤诱导氧化还原配偶体，范围为-150 mV至-500 mV的单电子氧化还原电位，以及任选的适当的缓冲系统。此外，通过NAD（P）H和过氧化氢将氧还原为过氧化氢的NAD（P）H和NAD（P）H产生反应的测定方法也以知识的方式测定，其中还原通过NAD（P）H的氧在NAD（P）H依赖性，不可自动氧化的心肌黄酶的存在下进行，其具有范围为0的双电子氧化还原电位的合适的双电子氧化还原配偶体 mV至150 mV以及适当的缓冲系统。另外，用于进行这些处理的试剂。

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