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    • 8. 发明申请
    • THE METHOD OF IMPROVING FLOTATION EFFICIENCY IN CARBONATED MINERALS WITH THE USE OF BIOTOATER
    • WO2022071903A2
    • 2022-04-07
    • PCT/TR2021/050839
    • 2021-08-20
    • ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİESKİŞEHİR TEKNİK ÜNİVERSİTESİ
    • ÖZ AKSOY, DeryaAYTAR ÇELİK, PinarKOCA, SabihaÇABUK, AhmetKOCA, HüseyinÇAKMAK, Hasan
    • C22B3/18C02F1/24C02F1/66C02F2103/10C02F2305/04C12N1/00
    • The present invention relates to the fields of ore enrichment, flotation, enrichment of carbonated ores/calcite-magnesite, biotechnology, microbiology, bioflotation. Flotation of carbonate minerals with the mining companies, who can enrich the use of sodium/potassium oleate chemical and chemicals, including chemicals that are produced by replace engaged in the production of flotation and flotation, especially to increase the efficiency characterized the chemical structure of a biological surface-active reagents to improve the use is whether the collector of property; nutrient agar (G/L meat extract 1 g, peptone 5 g, Sodium Chloride 5 g, yeast extract 2 g, agar 15 g and passivation of stock cultures of bacteria Bacillus subtilis (10), 24-hour, 35 °C, 150 rpm in nutrient agar enviroment (G/L meat extract 1, peptone 5 g, Sodium Chloride 5 g, yeast extract 2 g release the incubation (20), sterilized mineral salt medium (G/L; ammonium chloride 15 g, potassium dihydrogen phosphate 4.3 g, dipotassium hydrogen phosphate 3.4 g, potassium chloride 1.1 g, Sodium Chloride 1.1 g, yeast extract 0.5 g, Calcium Chloride 0.24 g, zinc sulfate synthesis 0.29 g, manganese sulfate monohydrate 0.17 g, magnesium sulfate synthesis 0.5 g, glucose 20 g of culture vaccine 2% to be inoculated with (30), 35°C and at 150 rpm release for at least 72 hours incubation (20) the preparation of raw bioreactive the result (40), after incubation of microorganisms growing in a mineral salt environment at +4 °C and supernatant was removed from the enviromet by centrifugation at 10,000 rpm for 15 minutes at least obtained (50), supernatant 's 2N HCL be reduced to PH 2 (60), and were transferred to centrifuge tubes at +4 °C for 10 minutes and centrifuged at 10,000 rpm (70), and pellets discharged on supernatantin the addition of ethyl acetate (80), pellets vortexation till it dissolves thoroughly up to (90), and were transferred to centrifuge tubes at +4°C can be centrifuged at 10,000 rpm for 10 minutes (70), separate a tube of dried supernatant taken into centrifuge concentrator (100), Fourier transform infrared spectroscopy analysis of the obtained alternately biosurfectan (110), followed by nuclear magnetic resonance analysis obtained biosurfectan (120), calcite/carbonate flotation the flotation of the samples used in the experiments suitable for size (0.150 mm) milling with grinding closed circuit (130), calcite of a sample of X-ray diffraction analysis (140), x- ray fluorescence spectrometry, element analysis of the samples by minor calcite (150), adding the produced bioreactive to the calcite (CaC03) flotation pulp as a collector (160) consists of the process steps of collecting the floating product (170) by giving air for a sufficient.