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1. 发明申请

US20030180953A1 Gene disruption methodologies for drug target discovery 审中-公开
标题翻译：药物靶点发现的基因破坏方法
公开(公告)号：US20030180953A1
公开(公告)日：2003-09-25
申请号：US10032585
申请日：2001-12-20
申请人： Elitra Pharmaceuticals, Inc.
发明人： Terry Roemer , Bo Jiang , Charles Boone , Howard Bussey , Kari L. Ohlsen
IPC分类号： C12N001/18 , C12N001/16 , C12N015/74
CPC分类号： C12N15/1082 , C07K14/37 , C07K14/40 , C12R1/645 , C12R1/725
摘要： The present invention provides methods and compositions that enable the experimental determination as to whether any gene in the genome of a diploid pathogenic organism is essential, and whether it is required for virulence or pathogenicity. The methods involve the construction of genetic mutants in which one allele of a specific gene is inactivated while the other allele of the gene is placed under conditional expression. The identification of essential genes and those genes critical to the development of virulent infections, provides a basis for the development of screens for new drugs against such pathogenic organisms. The present invention further provides Candida albicans genes that are demonstrated to be essential and are potential targets for drug screening. The nucleotide sequence of the target genes can be used for various drug discovery purposes, such as expression of the recombinant protein, hybridization assay and construction of nucleic acid arrays. The uses of proteins encoded by the essential genes, and genetically engineered cells comprising modified alleles of essential genes in various screening methods are also encompassed by the invention.
摘要翻译：本发明提供了能够对二倍体致病生物体的基因组中的任何基因是否必需的实验测定以及是否需要毒力或致病性的方法和组合物。该方法包括构建遗传突变体，其中特定基因的一个等位基因被灭活而基因的其他等位基因置于条件表达下。识别必需基因和对致病性感染发展至关重要的那些基因，为开发针对这种致病生物的新药筛选提供了基础。本发明进一步提供了被证实是必需的白念珠菌基因，并且是药物筛选的潜在靶标。靶基因的核苷酸序列可以用于各种药物发现目的，例如重组蛋白的表达，杂交测定和核酸阵列的构建。在各种筛选方法中，由必需基因编码的蛋白质的使用以及包含必需基因的修饰的等位基因的遗传工程改造的细胞也包括在本发明中。

2. 发明申请

US20020045592A1 Genes identified as required for proliferation in escherichia coli 审中-公开
标题翻译：在大肠杆菌中鉴定为增殖所需的基因
公开(公告)号：US20020045592A1
公开(公告)日：2002-04-18
申请号：US09912020
申请日：2001-07-23
申请人： ELITRA PHARMACEUTICALS, INC.
发明人： Judith Zyskind , Kari L. Ohlsen , John Trawick , R. Allyn Forsyth , Jamie M. Froelich , Grant J. Carr , Robert T. Yamamoto , H. Howard Xu
IPC分类号： A61K048/00 , C12N015/74
CPC分类号： C12Q1/18 , C07K14/245
摘要： The sequences of nucleic acids encoding proteins required for E. coli proliferation are disclosed. The nucleic acids can be used to express proteins or portions thereof, to obtain antibodies capable of specifically binding to the expressed proteins, and to use those expressed proteins as a screen to isolate candidate molecules for rational drug discovery programs. The nucleic acids can also be used to screen for homologous genes that are required for proliferation in microorganisms other than E. coli. The nucleic acids can also be used to design expression vectors and secretion vectors. The nucleic acids of the present invention can also be used in various assay systems to screen for proliferation required genes in other organisms as well as to screen for antimicrobial agents.
摘要翻译：公开了编码大肠杆菌增殖所需蛋白质的核酸序列。核酸可用于表达蛋白质或其部分，以获得能够特异性结合表达的蛋白质的抗体，并使用那些表达的蛋白质作为筛选来分离用于合理的药物发现程序的候选分子。核酸也可用于筛选除大肠杆菌以外的微生物中增殖所需的同源基因。核酸也可用于设计表达载体和分泌载体。本发明的核酸也可用于各种测定系统中以筛选其它生物体中的增殖需要的基因以及筛选抗微生物剂。

3. 发明专利

NZ521037A Gene disruption methodologies for drug target discovery 未知
公开(公告)号：NZ521037A
公开(公告)日：2004-09-24
申请号：NZ52103701
申请日：2001-02-20
申请人： ELITRA PHARMACEUTICALS INC
发明人： ROEMER TERRY , JIANG BO , BOONE CHARLES , BUSSEY HOWARD
IPC分类号： G01N33/50 , A61K31/7088 , A61K39/00 , A61K39/395 , A61K45/00 , A61K48/00 , A61P31/10 , C07K14/37 , C07K14/39 , C07K14/40 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/65 , C12N15/80 , C12N15/90 , C12P21/02 , C12Q1/02 , C12Q1/68 , C12R1/725 , G01N33/15 , G01N33/53 , G01N37/00 , C12N15/10 , C12P21/00 , C07K16/14
摘要： Methods and compositions that enable the experimental determination as to whether any gene in the genome of a diploid pathogenic organism is essential, and whether it is required for virulence or pathogenicity. The methods involve the construction of genetic mutants in which one allele of a specific gene is inactivated while the other allele of the gene is placed under conditional expression. The identification of essential genes and those genes critical to the development of virulent infections, provides a basis for the development of screens for new drugs against such pathogenic organisms. Further provided are Candida albicans genes that are demonstrated to be essential and are potential targets for drug screening. The nucleotide sequence of the target genes can be used for various drug discovery purposes, such as expression of the recombinant protein, hybridization assay and construction of nucleic acid arrays. The uses of proteins encoded by the essential genes, and genetically engineered cells comprising modified alleles of essential genes in various screening methods are also encompassed by the invention.

4. 发明专利

AU1824101A Nucleotide sequences of moraxella catarrhalis genome 未知
公开(公告)号：AU1824101A
公开(公告)日：2001-01-09
申请号：AU1824101
申请日：2000-06-16
申请人： ELITRA PHARMACEUTICALS INC
发明人： LAGACE ROBERT E , PATTERSON CHANDRA , BERG KIM L
IPC分类号： C07K14/21 , C12N15/31 , G01N33/50 , C12P21/00 , C12Q1/68 , C12N15/11 , C07H21/00
摘要： The present invention provides the genomic sequences of a library of purified nucleic acid molecules, or their complements, comprising the genome of Moraxella catarrhalis . The invention also provides the identification of open reading frames contained within the nucleic acid molecules of the library. The present invention further provides for the use of the nucleic acid molecules, their complements or fragments, and proteins or portions thereof for identifying ligands and useful diagnostic and therapeutic compositions. In addition the invention provides for vectors, host cells and methods for producing M. catarrhalis proteins or portions thereof.

5. 发明专利

AU2003297399A1 NUCLEIC ACIDS ENCODING ANTIFUNGAL DRUG TARGETS AND METHODS OF USE 未知
公开(公告)号：AU2003297399A1
公开(公告)日：2004-07-14
申请号：AU2003297399
申请日：2003-12-19
申请人： ELITRA PHARMACEUTICALS INC
发明人： ROEMER TERRY , JIANG BO , BOONE CHARLES , BUSSEY HOWARD
IPC分类号： A61K38/00 , C07K14/40 , C12N1/18 , C12N15/74 , C12N15/81

6. 发明专利

CA2445179A1 IDENTIFICATION OF ESSENTIAL GENES OF ASPEGILLUS FUMIGATUS AND METHODS OF USE 未知
公开(公告)号：CA2445179A1
公开(公告)日：2002-10-31
申请号：CA2445179
申请日：2002-04-23
申请人： ELITRA PHARMACEUTICALS INC
发明人： ZAMUDIO CARLOS , TISHKOFF DANIEL , EROSHKIN ALEXEY M , JIANG BO , HU WENQI , LEMIEUX SEBASTIEN M
IPC分类号： G01N33/50 , A61K31/7088 , A61K31/7105 , A61K38/00 , A61K39/395 , A61K45/00 , A61K48/00 , A61P31/04 , C07K14/37 , C07K14/38 , C07K16/14 , C07K19/00 , C12M1/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12P21/02 , C12P21/08 , C12Q1/02 , C12Q1/68 , G01N33/15 , G01N33/53 , G01N33/566 , C12N15/31 , C12P21/00 , C12N15/11 , C12N1/14 , A61K38/43 , C12N15/63
摘要： The present invention provides nucleotide sequences, methods and composition s that enable the experimental determination as to whether any gene in the genome of Aspergillus fumigatus is essential, and whether that gene is required for virulence or pathogenicity. The methods involve the constructio n of genetic mutants in which a target gene is placed under conditional expression. The identification of essential genes and those genes critical t o the development of virulent infections, provides a basis for the development of screens for new drugs against Aspergillus fumigatus. The present inventio n further provides Aspergillus fumigatus genes that are essential and are potential targets for drug screening. The nucleotide sequence of the target genes can be used for various drug discovery purposes, such as expression of the recombinant protein, hybridization assay and construction of nucleic aci d arrays. The uses of proteins encoded by the essential genes, and genetically engineered cells comprising modified alleles of essential genes in various screening methods are also encompassed by the invention.

7. 发明专利

AU2002306849A1 未知
公开(公告)号：AU2002306849A1
公开(公告)日：2002-10-08
申请号：AU2002306849
申请日：2002-03-21
申请人： ELITRA PHARMACEUTICALS INC
IPC分类号： C07K14/195 , C07K14/20 , C07K14/205 , C07K14/21 , C07K14/22 , C07K14/235 , C07K14/24 , C07K14/245 , C07K14/25 , C07K14/255 , C07K14/26 , C07K14/265 , C07K14/285 , C07K14/295 , C07K14/30 , C07K14/31 , C07K14/315 , C07K14/32 , C07K14/33 , C07K14/34 , C07K14/35 , C07K14/355 , C07K14/37 , C07K14/38 , C07K14/40

8. 发明专利

CA2398861A1 GENE DISRUPTION METHODOLOGIES FOR DRUG TARGET DISCOVERY 未知
公开(公告)号：CA2398861A1
公开(公告)日：2001-08-23
申请号：CA2398861
申请日：2001-02-20
申请人： ELITRA PHARMACEUTICALS INC
发明人： BUSSEY HOWARD , ROEMER TERRY , JIANG BO , BOONE CHARLES
IPC分类号： G01N33/50 , A61K31/7088 , A61K39/00 , A61K39/395 , A61K45/00 , A61K48/00 , A61P31/10 , C07K14/37 , C07K14/39 , C07K14/40 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/65 , C12N15/80 , C12N15/90 , C12P21/02 , C12Q1/02 , C12Q1/68 , C12R1/725 , G01N33/15 , G01N33/53 , G01N37/00 , C12P21/00 , C12N15/10 , C07K16/14
摘要： The present invention provides methods and compositions that enable the experimental determination as to whether any gene in the genome of a diploid pathogenic organism is essential, and whether it is required for virulence o r pathogenicity. The methods involve the construction of genetic mutants in which one allele of a specific gene is inactivated while the other allele of the gene is placed under conditional expression. The identification of essential genes and those genes critical to the development of virulent infections, provides a basis for the development of screens for new drugs against such pathogenic organisms. The present invention further provides Candida albicansgenes that are demonstrated to be essential and are potential targets for drug screening. The nucleotide sequence of the target genes can be used for various drug discovery purposes, such as expression of the recombinant protein, hybridization assay and construction of nucleic aci d arrays. The uses of proteins encoded by the essential genes, and genetically engineered cells comprising modified alleles of essential genes in various screening methods are also encompassed by the invention.

9. 发明专利

AU6754700A Chitobiase as a reporter enzyme 未知
公开(公告)号：AU6754700A
公开(公告)日：2001-04-23
申请号：AU6754700
申请日：2000-08-02
申请人： ELITRA PHARMACEUTICALS INC
发明人： ZYSKIND JUDITH
IPC分类号： C12N9/24 , C12Q1/68 , C12Q1/6897
摘要： The present invention relates to reporter gene constructs encoding a cytoplasmic form of chitobiase (N,N'-diacetylchitobiase) and methods of using these reporter gene constructs. The use of a cytoplasmic form of chitobiase as a reporter enzyme is generally applicable to the study of gene expression in organisms which do not contain N-acetyl- beta -D-glucosaminidases.

10. 发明专利

AU3584500A Genes identified as required for proliferation in (escherichia coli) 未知
公开(公告)号：AU3584500A
公开(公告)日：2000-08-18
申请号：AU3584500
申请日：2000-01-27
申请人： ELITRA PHARMACEUTICALS INC
发明人： ZYSKIND JUDITH , OHLSEN KARI L , TRAWICK JOHN , FORSYTH R ALLYN , FROELICH JAMIE M , CARR GRANT J , YAMAMOTO ROBERT T , XU H HOWARD
IPC分类号： G01N33/50 , A61K31/7088 , A61K48/00 , A61P31/04 , C07K14/245 , C07K16/12 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/31 , C12P21/02 , C12Q1/02 , C12Q1/68 , G01N33/15 , G01N33/53 , G01N33/566 , C12N15/11 , C12N15/10
摘要： The sequences of nucleic acids encoding proteins required for E. coli proliferation are disclosed. The nucleic acids can be used to express proteins or portions thereof, to obtain antibodies capable of specifically binding to the expressed proteins, and to use those expressed proteins as a screen to isolate candidate molecules for rational drug discovery programs. The nucleic acids can also be used to screen for homologous genes that are required for proliferation in microorganisms other than E. coli. The nucleic acids can also be used to design expression vectors and secretion vectors. The nucleic acids of the present invention can also be used in various assay systems to screen for proliferation required genes in other organisms as well as to screen for antimicrobial agents.

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