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1. 发明申请

US20210054441A1 Ultra-Sensitive Platform for Nucleic acid detection using a novel method, Scanning Digital polymerase chain reaction (PCR) 有权
公开(公告)号：US20210054441A1
公开(公告)日：2021-02-25
申请号：US17093429
申请日：2020-11-09
申请人： E.I. Spectra, LLC
发明人： Harold E. Ayliffe , Donald O'Neil
IPC分类号： C12Q1/686 , C12Q1/6848 , C12Q1/6827
摘要： A method for analyzing a target nucleic acid includes diluting nucleic acid targets and filling pico to femto-liter sized wells such that they contain a single target nucleic acid and one or more amplification reagents, amplifying the target in the individual wells, distinguishing wells containing amplicon from the target and amplicon from a variant of the target generated by polymerase error by using two differently labeled-hybridization probes, one hybridizing to the target and one hybridizing to a specific variant of the target; and analyzing target amplicons.

2. 发明申请

US20180335378A1 FLUORESCENCE FLOW CYTOMETRY DEVICE AND METHOD 审中-公开
公开(公告)号：US20180335378A1
公开(公告)日：2018-11-22
申请号：US15954260
申请日：2018-04-16
申请人： E.I. Spectra, LLC
发明人： Harold E. Ayliffe
IPC分类号： G01N15/14 , G01N21/64
CPC分类号： G01N15/1484 , B01L3/502715 , B01L2300/0654 , B01L2300/0816 , B01L2300/0887 , G01N15/1056 , G01N15/12 , G01N15/1436 , G01N15/1459 , G01N21/64 , G01N21/645 , G01N2015/1006 , G01N2015/1037 , G01N2201/06113
摘要： A flow cytometer including a laser, indexing structure, adjustment structure, and sensor structure. The cytometer is conventionally used with a removable microfluidic cassette, which is installed at a first position that is enforced by the indexing structure. The adjustment structure changes a relative position between an interrogation aperture of the cassette and the laser beam. Feedback from the sensor structure is used to optimize propagation of the laser through the interrogation aperture to reduce (and hopefully eliminate) autofluorescence caused by beam impingement onto the cassette.

3. 发明申请

US20190136296A1 Ultra-Sensitive Platform for Nucleic acid detection using a novel method, Scanning Digital polymerase chain reaction (PCR) 审中-公开
公开(公告)号：US20190136296A1
公开(公告)日：2019-05-09
申请号：US16184863
申请日：2018-11-08
申请人： E.I. Spectra, LLC
发明人： Harold E. Ayliffe , Donald O'Neil
IPC分类号： C12Q1/686 , C12Q1/6848
摘要： A method for analyzing a target nucleic acid includes diluting nucleic acid targets and filling pico to femto-liter sized wells such that they contain a single target nucleic acid and one or more amplification reagents, amplifying the target in the individual wells, distinguishing wells containing amplicon from the target and amplicon from a variant of the target generated by polymerase error by using two differently labeled-hybridization probes, one hybridizing to the target and one hybridizing to a specific variant of the target; and analyzing target amplicons.

4. 发明授权

US11891657B2 Ultra-sensitive platform for nucleic acid detection using a novel method, scanning digital polymerase chain reaction (PCR) 有权
公开(公告)号：US11891657B2
公开(公告)日：2024-02-06
申请号：US17093429
申请日：2020-11-09
申请人： E.I. Spectra, LLC
发明人： Harold E. Ayliffe , Donald O'Neil
IPC分类号： C12Q1/686 , C12Q1/6848 , C12Q1/6827
CPC分类号： C12Q1/686 , C12Q1/6827 , C12Q1/6848 , C12Q1/6827 , C12Q2527/146 , C12Q2563/159
摘要： A method for analyzing a target nucleic acid includes diluting nucleic acid targets and filling pico to femto-liter sized wells such that they contain a single target nucleic acid and one or more amplification reagents, amplifying the target in the individual wells, distinguishing wells containing amplicon from the target and amplicon from a variant of the target generated by polymerase error by using two differently labeled-hybridization probes, one hybridizing to the target and one hybridizing to a specific variant of the target; and analyzing target amplicons.

5. 发明申请

US20180335376A1 MICROFLUIDIC INTERROGATION DEVICE 审中-公开
公开(公告)号：US20180335376A1
公开(公告)日：2018-11-22
申请号：US15953302
申请日：2018-04-13
申请人： E.I. Spectra, LLC
发明人： Harold E. Ayliffe
IPC分类号： G01N15/14 , H01J49/00 , B01L3/00 , G01N15/10 , G01N15/12 , G01N27/00 , G01N35/00 , B01L99/00 , G01N33/49 , G06F19/00 , G06F17/40
摘要： A portable, stand-alone microfluidic interrogation device including a microprocessor and a touch-screen display. The touch-screen display can receive one or more user input to select a particular particle interrogation procedure, and subsequently show interrogation results. A microfluidic path extending through the interrogation device includes alignment structure that defines an interrogation zone in which particles carried in a fluid are urged toward single-file travel. Operable alignment structure may define sheath-, or non-sheath fluid flow. Desirably, a portion of the alignment structure is removable from the device in a tool-free procedure. The device may operate under the Coulter principle, and/or detect Stokes' shift phenomena, and/or other optically-based signal(s).

6. 发明申请

WO2012158207A2 METHOD FOR MUTIPLEXED MICROFLUIDIC BEAD-BASED IMMUNOASSAY 审中-公开
标题翻译：基于微流控粒径的免疫荧光法的方法
公开(公告)号：WO2012158207A2
公开(公告)日：2012-11-22
申请号：PCT/US2012/000237
申请日：2012-05-07
申请人： E.I. SPECTRA, LLC , AYLIFFE, Harold, E.
发明人： AYLIFFE, Harold, E.
IPC分类号： G01N33/546
CPC分类号： G01N15/12 , G01N15/1459 , G01N15/1484 , G01N2015/1037
摘要： A method for performing multiplexed bead-based immunoassays using a microfluidic cassette capable of detecting a particle passing in substantially single file through an interrogation zone and generating a Coulter effect signal responsive to a characteristic of the particle. A fluid sample may be prepared by associating antibody-coated beads of different sizes to particles of interest. A first multiplexing option may be based on bead size, in which case the intensity of the Coulter signal is used to sort or characterize the particles. A second multiplexing option may be based on detection of Stokes' shift phenomena, or even simply emission intensity, in which case particles may be characterized responsive to intensity of the signal resulting from detection of radiation. The first and second multiplexing options may be employed together to populate an array of particle characteristics.
摘要翻译：一种使用微流控盒进行多重珠粒免疫测定的方法，所述微流控盒能够检测通过询问区通过基本单个文件的颗粒，并响应于颗粒的特征产生库尔特效应信号。可以通过将不同大小的抗体包被的珠粒与感兴趣的颗粒相关联来制备流体样品。第一复用选项可以基于焊道尺寸，在这种情况下，Coulter信号的强度用于对颗粒进行分类或表征。第二复用选项可以基于斯托克斯移位现象的检测，或者甚至是发射强度，在这种情况下，可以根据由辐射检测产生的信号的强度来表征粒子。可以一起采用第一和第二复用选项来填充粒子特征的阵列。

7. 发明申请

WO2009126257A1 METHOD FOR MANUFACTIRING A MICROFLUIDIC SENSOR 审中-公开
标题翻译：微流感传感器的制造方法
公开(公告)号：WO2009126257A1
公开(公告)日：2009-10-15
申请号：PCT/US2009/002172
申请日：2009-04-07
申请人： E.I. SPECTRA, LLC , AYLIFFE, Harold, E. , KING, Curtis, S.
发明人： AYLIFFE, Harold, E. , KING, Curtis, S.
IPC分类号： G01N11/00
CPC分类号： B01L3/502707 , B01L2200/0647 , B01L2200/10 , B01L2300/0645 , B01L2300/0681 , B01L2300/0874 , B01L2300/0887 , B01L2300/123
摘要： A method to manufacture microfluidic sensors, typically including componentizing substrate layers One such method includes providing a plurality of layers of material configured to permit their stacking to form at least a first cap layer, a first channel layer, an interrogation layer, and a second channel layer During assembly, ribbon sections of substrate layers are sandwiched to cooperatively align elements through-the-thickness of the sandwich Individual sensors are then removed from the sandwich ribbon A componentizing step includes forming one or more elements for successive sensors spaced along the axial length of a ribbon Certain elements include electrically conductive patterned structures preferably printed onto a substrate using conductive ink and a printing process, sometimes placing material in operable position to conduct electricity through the thickness of at least one nbbon Other elements may include channels, tunnels, and vias that can be machined, stamped, or cut into a ribbon section.
摘要翻译：一种制造微流体传感器的方法，通常包括组分化衬底层一种这样的方法包括提供多层材料，其被配置为允许其堆叠形成至少第一覆盖层，第一沟道层，询问层和第二沟道层在组装期间，衬底层的带状部分被夹持以通过夹层的厚度协同对准元件。然后将单独的传感器从夹带中移出。组件化步骤包括形成一个或多个元件，用于沿着轴向长度丝带某些元件包括导电图案结构，优选地使用导电油墨和印刷方法印刷在基底上，有时将材料放置在可操作位置以将电穿过至少一个nbbon的厚度。其它元件可以包括通道，隧道和通孔，可加工，冲压或切割丝带部分。

8. 发明申请

WO2013184168A1 FLUORESCENCE FLOW CYTOMETRY 审中-公开
标题翻译：荧光细胞计数
公开(公告)号：WO2013184168A1
公开(公告)日：2013-12-12
申请号：PCT/US2013/000145
申请日：2013-06-05
申请人： E.I. SPECTRA, LLC
发明人： AYLIFFE, Harold, E.
IPC分类号： G01N21/64
CPC分类号： G01N21/64 , B01L3/502715 , B01L2300/0654 , B01L2300/0816 , G01N15/1056 , G01N15/12 , G01N15/1459 , G01N15/1484 , G01N2015/1006 , G01N2015/1037
摘要： A flow cytometer (100) including a laser (134), indexing structure (102), adjustment structure (144), and sensor structure (190). The cytometer is conventionally used with a removable microfluidic cassette (104), which is installed at a first position that is enforced by the indexing structure. The adjustment structure changes a relative position between an interrogation aperture of the cassette and the laser beam (132). Feedback from the sensor structure is used to optimize propagation of the laser through the interrogation aperture (106) to reduce (and hopefully eliminate) autofluorescence caused by beam impingement onto the cassette (104).
摘要翻译：包括激光器（134），分度结构（102），调节结构（144）和传感器结构（190）的流式细胞仪（100）。通常，细胞计数器与可拆卸的微流体盒（104）一起使用，其可安装在由分度结构执行的第一位置处。调整结构改变了盒的询问孔与激光束（132）之间的相对位置。来自传感器结构的反馈用于优化激光通过询问孔（106）的传播，以减少（并希望消除）由光束撞击到盒（104）上引起的自发荧光。

9. 发明公开

EP2859332A1 FLUORESCENCE FLOW CYTOMETRY 有权转让
标题翻译：荧光流式细胞仪
公开(公告)号：EP2859332A1
公开(公告)日：2015-04-15
申请号：EP13800086.4
申请日：2013-06-05
申请人： E.I. Spectra, LLc
发明人： AYLIFFE, Harold, E.
IPC分类号： G01N21/64
CPC分类号： G01N21/64 , B01L3/502715 , B01L2300/0654 , B01L2300/0816 , G01N15/1056 , G01N15/12 , G01N15/1459 , G01N15/1484 , G01N2015/1006 , G01N2015/1037
摘要： A flow cytometer (100) including a laser (134), indexing structure (102), adjustment structure (144), and sensor structure (190). The cytometer is conventionally used with a removable microfluidic cassette (104), which is installed at a first position that is enforced by the indexing structure. The adjustment structure changes a relative position between an interrogation aperture of the cassette and the laser beam (132). Feedback from the sensor structure is used to optimize propagation of the laser through the interrogation aperture (106) to reduce (and hopefully eliminate) autofluorescence caused by beam impingement onto the cassette (104).

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