专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20060192107A1 Methods and apparatus for porous membrane electrospray and multiplexed coupling of microfluidic systems with mass spectrometry 审中-公开
标题翻译：多孔膜电喷雾的方法和装置以及微流体系统与质谱的多重耦合
公开(公告)号：US20060192107A1
公开(公告)日：2006-08-31
申请号：US11242842
申请日：2005-10-05
申请人： Donald DeVoe , Yingxin Wang , Cheng Lee , Yan Li
发明人： Donald DeVoe , Yingxin Wang , Cheng Lee , Yan Li
IPC分类号： B01D59/44
CPC分类号： B05B5/025 , G01N27/44717 , G01N30/7266
摘要： Disclosed are an apparatus, system, and method for performing electrospray of biomolecules, particularly peptides, polypeptides, and proteins. The apparatus comprises at least (1) a microfluidic substrate for containing an electrospray microchannel for delivering analyte molecules to a side edge of the substrate, and (2) a porous membrane attached to the side edge for performing electrospray from the exposed membrane surface. In one preferred embodiment, the exposed membrane surface is positioned above a target surface for depositing analyte molecules onto the target surface by electrospray. In another preferred embodiment, a proteolytic enzyme is bound to the porous membrane for performing protein digestion during electrospray.
摘要翻译：公开了用于进行生物分子，特别是肽，多肽和蛋白质的电喷雾的装置，系统和方法。该装置至少包括（1）微流体衬底，用于容纳用于将分析物分子输送到衬底的侧边缘的电喷射微通道，和（2）附着到侧边缘的用于从暴露的膜表面进行电喷雾的多孔膜。在一个优选实施例中，暴露的膜表面位于靶表面上方，用于通过电喷射将分析物分子沉积到靶表面上。在另一个优选的实施方案中，蛋白水解酶与多孔膜结合，用于在电喷雾期间进行蛋白质消化。

2. 发明申请

US20060019399A1 Devices and methods for correlated analysis of multiple protein or peptide samples 审中-公开
标题翻译：多种蛋白质或肽样品相关分析的装置和方法
公开(公告)号：US20060019399A1
公开(公告)日：2006-01-26
申请号：US11171427
申请日：2005-07-01
申请人： Brian Balgley , Jonathan Cooper , Cheng Lee , Donald DeVoe
发明人： Brian Balgley , Jonathan Cooper , Cheng Lee , Donald DeVoe
IPC分类号： G01N33/00
CPC分类号： C07K1/16 , G01N30/466 , G01N35/1095 , G01N2030/8813 , H01J49/009 , H01J49/0431
摘要： Disclosed is a system for performing multiple analyses of protein and/or peptide samples and correlating the results of the analyses. The system comprises a sample inlet, a splitter means, at least two sample delivery capillaries, at least two sample deposition tools, and at least two sample collectors, wherein said splitter means is in fluid communication with the sample inlet and the sample delivery capillaries, and wherein liquid flow entering the splitter means is split into a number of sub-flows equal to the number of sample delivery capillaries. In one preferred embodiment, at least one microenzyme reactor is interfaced to a first sample delivery capillary in order to digest a protein sample within the capillary, while a second sample delivery capillary does not contain a microenzyme reactor, thereby enabling correlated analysis of the same protein sample in digested and undigested form. Methods for performing two or more analyses of protein and/or peptide samples and correlating the results of the analyses are also disclosed.
摘要翻译：公开了一种用于进行蛋白质和/或肽样品的多次分析并将分析结果相关联的系统。该系统包括样品入口，分离装置，至少两个样品输送毛细管，至少两个样品沉积工具和至少两个样品收集器，其中所述分离装置与样品入口和样品输送毛细管流体连通，并且其中进入分离装置的液体流被分成等于样品递送毛细管数量的多个子流。在一个优选的实施方案中，至少一个微酶反应器与第一样品递送毛细管连接，以便消化毛细管内的蛋白质样品，而第二样品递送毛细管不含有微酶反应器，从而能够进行相同蛋白质的相关分析采样消化和未消化形式。还公开了对蛋白质和/或肽样品进行两次或多次分析并关联分析结果的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式