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    • 6. 发明授权
    • Glyphosate-resistant plants
    • 抗草甘膦植物
    • US5188642A
    • 1993-02-23
    • US478794
    • 1990-02-12
    • Dilip M. ShahStephen G. RogersRobert B. HorschRobert T. Fraley
    • Dilip M. ShahStephen G. RogersRobert B. HorschRobert T. Fraley
    • A01H1/00A01H1/02A01H5/00C12N5/00C12N5/10C12N9/10C12N15/00C12N15/09C12N15/82
    • C12N15/8275
    • This invention involves a cloning or expression vector comprising a gene which encodes 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide which, when expressed in a plant cell contains a chloroplast transit peptide which allows the polypeptide, or an enzymatically active portion thereof, to be transported from the cytoplasm of the plant cell into a chloroplast in the plant cell, and confers a substantial degree of glyphosate resistance upon the plant cell and plants regenerated therefrom.The EPSPS coding sequence may be ligated to a strong promoter, such as the 35S promoter from cauliflower mosaic virus, to create a chimeric gene. Such genes can be inserted into plant transformation vectors, and subsequently introduced into plant cells. Plant cells transformed using such genes and plants regenerated therefrom have been shown to exhibit a substantial degree of glyphosate resistance.
    • 本发明涉及包含编码5-烯醇丙酮酸莽草酸-3-磷酸合成酶(EPSPS)多肽的基因的克隆或表达载体,其在植物细胞中表达时含有允许多肽的叶绿体转运肽或其酶活性部分, 从植物细胞的细胞质转移到植物细胞中的叶绿体中,并且赋予植物细胞和植物再生的植物大量的草甘膦抗性。 EPSPS编码序列可以连接到强启动子,例如来自花椰菜花叶病毒的35S启动子,以产生嵌合基因。 可将这些基因插入植物转化载体中,随后导入植物细胞。 已经显示使用从其再生的这些基因和植物转化的植物细胞表现出相当程度的草甘膦抗性。