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1. 发明授权

US08889416B2 Methods and devices for micro-isolation, extraction, and/or analysis of microscale components 有权
标题翻译：微量元件的微分离，提取和/或分析的方法和装置
公开(公告)号：US08889416B2
公开(公告)日：2014-11-18
申请号：US13010761
申请日：2011-01-20
申请人： Emil P. Kartalov , Darryl Shibata , Clive Taylor , Lawrence A. Wade
发明人： Emil P. Kartalov , Darryl Shibata , Clive Taylor , Lawrence A. Wade
IPC分类号： C12N5/02 , C12N5/00 , C12M1/34 , C12N13/00 , G01N33/543
CPC分类号： C12M23/16 , C12M23/12 , C12M23/22 , C12M25/04 , C12M25/14 , C12M35/02 , C12M37/04 , C12M47/04 , C12N13/00 , G01N33/54366
摘要： Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation apparatus described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation apparatus can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation apparatus can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation apparatus can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device. Also included are methods for selectively isolating cellular material using the apparatuses described herein, as are methods for biochemical analysis of individual regions of interest of cellular material using the devices described herein. Further included are methods of making masking arrays useful for the methods described herein.
摘要翻译：本文提供用于微生物细胞材料的微分离的装置和方法。所描述的微分离装置可以包括保护感兴趣区域以防止DNA破坏照明的光掩模。微型隔离装置还可以包括感光材料，该感光材料在照射之后界定存取孔，随后显影感光材料。微隔离装置还可以包括腔室微流体装置，其包括通道，其提供对感光材料中限定的孔的通路。微型隔离装置可以包括腔室微流体装置，其无感光材料中限定的通路孔，阀门通过微流体装置的通道控制气体或液体的流动。还包括使用本文所述装置选择性分离细胞材料的方法，以及使用本文所述装置的细胞材料的各个感兴趣区域的生物化学分析的方法。还包括制备用于本文所述方法的掩蔽阵列的方法。

2. 发明申请

US20110177518A1 METHODS AND DEVICES FOR MICRO-ISOLATION, EXTRACTION, AND/OR ANALYSIS OF MICROSCALE COMPONENTS 有权
标题翻译： MICROSCALE组件的微分离，提取和/或分析的方法和设备
公开(公告)号：US20110177518A1
公开(公告)日：2011-07-21
申请号：US13010761
申请日：2011-01-20
申请人： Emil P. Kartalov , Darryl Shibata , Clive Taylor , Lawrence A. Wade
发明人： Emil P. Kartalov , Darryl Shibata , Clive Taylor , Lawrence A. Wade
IPC分类号： C12Q1/68 , C12M1/00 , C12N13/00 , C12Q1/02 , G01N33/53 , G03F7/20
CPC分类号： C12M23/16 , C12M23/12 , C12M23/22 , C12M25/04 , C12M25/14 , C12M35/02 , C12M37/04 , C12M47/04 , C12N13/00 , G01N33/54366
摘要： Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation apparatus described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation apparatus can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation apparatus can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation apparatus can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device. Also included are methods for selectively isolating cellular material using the apparatuses described herein, as are methods for biochemical analysis of individual regions of interest of cellular material using the devices described herein. Further included are methods of making masking arrays useful for the methods described herein.
摘要翻译：本文提供用于微生物细胞材料的微分离的装置和方法。所描述的微分离装置可以包括保护感兴趣区域以防止DNA破坏照明的光掩模。微型隔离装置还可以包括感光材料，该感光材料在照射之后界定存取孔，随后显影感光材料。微隔离装置还可以包括腔室微流体装置，其包括通道，其提供对感光材料中限定的孔的通路。微型隔离装置可以包括腔室微流体装置，其无感光材料中限定的通路孔，阀门通过微流体装置的通道控制气体或液体的流动。还包括使用本文所述的装置选择性分离细胞材料的方法，以及使用本文所述的装置的细胞材料的各个感兴趣区域的生物化学分析的方法。还包括制备用于本文所述方法的掩蔽阵列的方法。

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