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    • 3. 发明申请
    • Ploidy classification method
    • 倍性分类法
    • US20050272073A1
    • 2005-12-08
    • US11097451
    • 2005-04-01
    • Eugeni VaisbergDaniel Coleman
    • Eugeni VaisbergDaniel Coleman
    • C12Q1/68G01N33/48G01N33/50G06F19/00
    • G01N33/5005
    • Image analysis methods and apparatus are used for determination of the ploidy of cells. The methods may involve segmenting an image to identify one or more discrete regions occupied by cells or nuclei, determining the presence of a particular ploidy indicator feature within the region(s), and providing a value of the indicator feature to a model that classifies cells' ploidy on the basis of the indicator feature. In some embodiments, the indicator feature is a level of DNA in a cell. In certain embodiments, the method further comprises treating one or more cells with a marker that highlights the ploidy indicator feature. In certain embodiments, the cells are treated prior to producing one or more images of the one or more cells. In certain embodiments, the ploidy indicator feature comprises DNA and the marker co-locates with DNA and provides a signal that is captured in the image. In certain embodiments, the signal comprises a fluorescent emission.
    • 图像分析方法和装置用于确定细胞的倍性。 所述方法可以包括分割图像以识别由单元或核占据的一个或多个离散区域,确定区域内特定倍性指示符特征的存在,以及将指示符特征的值提供给将细胞分类的模型 在指标特征的基础上进行倍性。 在一些实施方案中,指示剂特征是细胞中的DNA水平。 在某些实施方案中,所述方法还包括用突出所述倍性指示物特征的标记物处理一个或多个细胞。 在某些实施方案中,在产生一个或多个细胞的一个或多个图像之前处理细胞。 在某些实施方案中,倍性指示物特征包括DNA,并且标记物与DNA共定位并提供在图像中捕获的信号。 在某些实施例中,信号包括荧光发射。
    • 4. 发明授权
    • Ocular protective apparatus
    • 眼睛保护装置
    • US4786125A
    • 1988-11-22
    • US639661
    • 1984-08-13
    • Jose MagarinosDaniel Coleman
    • Jose MagarinosDaniel Coleman
    • A61F9/02G02B5/18G02B5/32G02C7/10G03H1/02
    • A61F9/022G03H2001/043G03H2270/21
    • A laser shield (FIG. 3) for providing ocular protection against laser hazards, while still providing high photopic or scotopic transmittance and good color discrimination is disclosed. This laser shield comprises a holographic optical notch filter mirror (102) having an optical surface such that the angle (104) made by an incoming laser beam (114), which is aimed by the pupil of the user, with respect to the normal of the optical surface is less than the corresponding angle (108) made by the same incoming laser beam (114) with respect to the physical surface of the substrate. This decrease in the effective angle of incidence reduces the spectral shift of the notch (which defines the frequency that is reflected), thereby providing increased protection for the same range of angles of incidence. The holographic mirror optical surface formed can be the analog of a conventional spherical, hyperbolic, parabolic, elliptical or other aspheric mirror.
    • 公开了一种用于提供针对激光危险的眼睛保护的激光屏蔽(图3),同时仍然提供高的明视或透视透光率和良好的颜色鉴别。 该激光屏包括具有光学表面的全息光学陷波滤光镜(102),使得由用户瞳孔瞄准的入射激光束(114)制成的角度(104)相对于 光学表面小于由相同入射激光束(114)相对于衬底的物理表面制成的相应角度(108)。 有效入射角的这种降低降低了凹口的光谱偏移(其定义了被反射的频率),从而为相同入射角范围提供了更大的保护。 形成的全息镜光学表面可以是常规的球形,双曲线,抛物面,椭圆形或其他非球面镜的模拟。
    • 8. 发明申请
    • Methods and apparatus for characterising cells and treatments
    • 用于表征细胞和治疗的方法和装置
    • US20050009032A1
    • 2005-01-13
    • US10615116
    • 2003-07-07
    • Daniel ColemanGe CongAibing RaoEugeni Vaisberg
    • Daniel ColemanGe CongAibing RaoEugeni Vaisberg
    • G06K9/00G06T7/00C12Q1/68
    • G06K9/0014G06T7/0012G06T2207/30024
    • Methods, data processing apparatus and computer program products for characterising cells and the affect of treatments administered to cells are disclosed. In particular methods of identifying bi-nuclear cells are described which include capturing an image of a plurality of marked cells and processing image to obtain features of the plurality of cells. The features are analyzed to determine whether the feature is indicative of bi-nuclear cells. Those cells for which the first feature is indicative of bi-nuclear cells are identified as being bi-nuclear. Three algorithms in particular are described. A first algorithm can be used to determine the number of nuclei in an image of a nuclear component by determining the number of concave regions within the outline of the image. A second algorithm uses a measure of the amount of cytoplasmic material between a pair of nuclei to identify bi-nuclear cells. A third algorithm uses the statistics of the spatial distribution of objects to identify isolated pairs of nuclei which can be considered to be from the same cell.
    • 公开了用于表征细胞的方法,数据处理装置和计算机程序产品以及对细胞施用的治疗的影响。 描述了特定的识别双核细胞的方法,其包括捕获多个标记细胞的图像和处理图像以获得多个细胞的特征。 分析特征以确定特征是否表示双核细胞。 第一特征指示双核细胞的那些细胞被鉴定为双核的。 具体描述三种算法。 可以使用第一算法来确定图像轮廓内的凹区域的数量来确定核分量的图像中的核的数量。 第二种算法使用一对核之间的细胞质材料的量的量度来识别双核细胞。 第三种算法使用对象的空间分布的统计来识别可以被认为来自相同小区的孤立的核对。