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1. 发明授权

US06303359B1 DNA molecule encoding new aminopeptidase, and method of producing the aminopeptidase 失效
标题翻译：编码新氨基肽酶的DNA分子，以及氨基肽酶的制备方法
公开(公告)号：US06303359B1
公开(公告)日：2001-10-16
申请号：US09525046
申请日：2000-03-14
申请人： Daiki Ninomiya , Tetsuya Miwa , Minao Asano , Nami Nakamura , Noriki Nio
发明人： Daiki Ninomiya , Tetsuya Miwa , Minao Asano , Nami Nakamura , Noriki Nio
IPC分类号： C12N948
CPC分类号： C12N9/48
摘要： Disclosed are cDNA encoding a new aminopeptidase derived from germinated soybeans, a recombinant expression vector containing the DNA, a transformant obtained by the transformation with the expression vector, and a method of producing an aminopeptidase by culturing the transformed product. According to the present invention, a cDNA can be obtained encoding the amino acid sequence of aminopeptidase GX suitable for producing a highly hydrolized product from a starting material containing a protein and peptide having a high acidic amino acid content. Using the cDNA, a recombinant aminopeptidase can be mass-produced with E. coli or the like. By using the cDNA, it is also possible to produce aminopeptidase GX by using a host other than E. coli. In addition, hydrohized products having high glutamic acid content and aspartic acid content and also excellent seasoning properties can be obtained from soybean protein by combining GX thus produced with protease D3 and leucine aminopeptidase DLAP.
摘要翻译：公开了编码来自发芽大豆的新氨基肽酶的cDNA，含有DNA的重组表达载体，通过用表达载体转化获得的转化体，以及通过培养转化产物来生产氨基肽酶的方法。根据本发明，可以获得编码氨肽酶GX的氨基酸序列的cDNA，该氨基酸序列适于从含有高酸性氨基酸含量的蛋白质和肽的原料制备高度水解的产物。使用该cDNA，重组氨基肽酶可以用大肠杆菌等大量生产。通过使用cDNA，也可以通过使用除大肠杆菌以外的宿主产生氨基肽酶GX。此外，通过将由此产生的GX与蛋白酶D3和亮氨酸氨基肽酶DLAP组合，可以从大豆蛋白获得具有高谷氨酸含量和天冬氨酸含量以及天冬氨酸含量高的水解产物。

2. 发明授权

US07087422B2 Aminopeptidase and the genes thereof 失效
标题翻译：氨基肽酶及其基因
公开(公告)号：US07087422B2
公开(公告)日：2006-08-08
申请号：US10664958
申请日：2003-09-22
申请人： Kyoko Koibuchi , Daiki Ninomiya , Mari Kojima , Yoichi Ueda , Jun-ichi Maruyama , Katsuhiko Kitamoto
发明人： Kyoko Koibuchi , Daiki Ninomiya , Mari Kojima , Yoichi Ueda , Jun-ichi Maruyama , Katsuhiko Kitamoto
IPC分类号： C12N9/62 , C12N15/57 , C12N15/70 , C12N15/81
CPC分类号： C12N9/62
摘要： The object of the present invention is to provide Koji mold aminopeptidases capable of efficiently hydrolyzing persistent peptides and also genes encoding the aminopeptidases. The present invention provides Aspergillus nidulans aminopeptidase and nucleic acid molecules encoding it. In particular, the present invention provides a protein having an amino acid sequence represented by amino acid Nos. 1 to 519 in SEQ ID NO: 2, or a protein containing the substitution, deletion, insertion, addition or inversion of one or more amino acids in said sequence, and which protein has an activity of catalyzing the reaction for releasing an amino acid at an N-terminal of a peptide, and nucleic acid molecules encoding them.
摘要翻译：本发明的目的是提供能够有效水解持久性肽的Koji模具氨基肽酶以及编码氨基肽酶的基因。本发明提供构巢曲霉氨基肽酶和编码它的核酸分子。特别地，本发明提供了具有SEQ ID NO：2中的氨基酸编号1〜519所示的氨基酸序列的蛋白质，或含有一个或多个氨基酸的取代，缺失，插入，添加或倒置的蛋白质并且哪个蛋白质具有催化用于释放肽的N末端的氨基酸的反应的活性和编码它们的核酸分子的活性。

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