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1. 发明申请

WO2010107816A2 NUCLEIC ACID BINDING DYES AND USES THEREFOR 审中-公开
标题翻译：核酸结合染色体及其用途
公开(公告)号：WO2010107816A2
公开(公告)日：2010-09-23
申请号：PCT/US2010/027514
申请日：2010-03-16
申请人： PROMEGA CORPORATION , MCDOUGALL, Mark , DWIGHT, Stephen
发明人： MCDOUGALL, Mark , DWIGHT, Stephen
IPC分类号： C12Q1/68 , C07D513/04 , C12N9/10
CPC分类号： C12Q1/686 , C07D513/06 , C09B23/04 , C12Q2563/107
摘要： The invention provides novel compounds and compositions of Formulas (I) and (II), as well as methods of using them. The compounds can be used, for example, to quantify an amount of double stranded DNA in a sample subjected to nucleic acid amplification, or for real time monitoring of a nucleic acid amplification reaction. The compounds can be provided in a kit, for example, with other reagents and instructions for using the compounds and reagents.
摘要翻译：本发明提供式（I）和（II）的新化合物和组合物，以及使用它们的方法。该化合物可用于例如进行核酸扩增的样品中的双链DNA量，或用于核酸扩增反应的实时监测。化合物可以在试剂盒中提供，例如用其它试剂和使用化合物和试剂的说明书。

2. 发明申请

WO2010107816A3 NUCLEIC ACID BINDING DYES AND USES THEREFOR 审中-公开
标题翻译：核酸结合染料及其用途
公开(公告)号：WO2010107816A3
公开(公告)日：2010-12-16
申请号：PCT/US2010027514
申请日：2010-03-16
申请人： PROMEGA CORP , MCDOUGALL MARK , DWIGHT STEPHEN
发明人： MCDOUGALL MARK , DWIGHT STEPHEN
IPC分类号： C09B23/04 , C09B23/06 , C12Q1/68
CPC分类号： C12Q1/686 , C07D513/06 , C09B23/04 , C12Q2563/107
摘要： The invention provides novel compounds and compositions of Formulas (I) and (II), as well as methods of using them. The compounds can be used, for example, to quantify an amount of double stranded DNA in a sample subjected to nucleic acid amplification, or for real time monitoring of a nucleic acid amplification reaction. The compounds can be provided in a kit, for example, with other reagents and instructions for using the compounds and reagents.
摘要翻译：本发明提供了式（I）和（II）的新化合物和组合物，以及使用它们的方法。例如，可以使用这些化合物来定量进行核酸扩增的样品中双链DNA的量或实时监测核酸扩增反应。化合物可以在试剂盒中提供，例如与其他试剂和使用化合物和试剂的说明书一起提供。

3. 发明授权

US07530509B2 Manure-grinding fertilizer spreader 失效
标题翻译：肥料撒肥机
公开(公告)号：US07530509B2
公开(公告)日：2009-05-12
申请号：US11487895
申请日：2006-07-17
申请人： Ricky L. Gaughan , Dwight Stephen Tabb
发明人： Ricky L. Gaughan , Dwight Stephen Tabb
IPC分类号： A01C15/00 , A01C19/00
CPC分类号： A01C3/06
摘要： An improved manure-grinding fertilizer spreader having at least two progressively bladed cylinders 17 at a bottom of a funneled mobile hopper 11 with a grinder base 12 proximate a discharge aperture 13. The mobile funneled hopper is hand-cart sized to be hand-pulled or small-tractor-pulled with at least two progressively bladed cylinders 17 being rotated by detachable engagement of wheels 15 with at least two cutter axles 10 and 56 on which the at least two progressively bladed cylinders 17 are situated axially. An optional agitator 47 may be used in conjunction with the improved manure-grinding fertilizer spreader so as to prevent manure build-up.
摘要翻译：一种改进的粪肥研磨肥料撒布机，其具有在漏斗的可移动料斗11的底部处的至少两个逐渐叶片的气缸17，研磨机基座12靠近排放孔13.移动漏斗的料斗是手推车的大小以手拉或小拖拉机拉动，其中至少两个逐渐叶片的气缸17通过轮15与至少两个渐进叶片气缸17轴向位于其上的至少两个切割轴10和56的可拆卸接合来旋转。任选的搅拌器47可以与改进的肥料 - 研磨肥料撒肥器一起使用，以防止粪便积聚。

4. 发明申请

US20080023504A1 Manure-grinding fertilizer spreader 失效
标题翻译：肥料撒肥机
公开(公告)号：US20080023504A1
公开(公告)日：2008-01-31
申请号：US11487895
申请日：2006-07-17
申请人： Ricky L. Gaughan , Dwight Stephen Tabb
发明人： Ricky L. Gaughan , Dwight Stephen Tabb
IPC分类号： A01C15/16
CPC分类号： A01C3/06
摘要： An improved manure-grinding fertilizer spreader having at least two progressively bladed cylinders 17 at a bottom of a funneled mobile hopper 11 with a grinder base 12 proximate a discharge aperture 13. The mobile funneled hopper is hand-cart sized to be hand-pulled or small-tractor-pulled with at least two progressively bladed cylinders 17 being rotated by detachable engagement of wheels 15 with at least two cutter axles 10 and 56 on which the at least two progressively bladed cylinders 17 are situated axially. An optional agitator 47 may be used in conjunction with the improved manure-grinding fertilizer spreader so as to prevent manure build-up.
摘要翻译：一种改进的粪肥研磨肥料撒布机，其具有在漏斗的可移动料斗11的底部处的至少两个逐渐叶片的气缸17，研磨机基座12靠近排放孔13.移动漏斗的料斗是手推车的大小以手拉或小拖拉机拉动，其中至少两个逐渐叶片的气缸17通过轮15与至少两个渐进叶片气缸17轴向位于其上的至少两个切割轴10和56的可拆卸接合来旋转。任选的搅拌器47可以与改进的肥料 - 研磨肥料撒肥器一起使用，以防止粪便积聚。

5. 发明授权

US06321954B1 Manure grinding fertilizer spreader 有权
标题翻译：肥料撒肥机
公开(公告)号：US06321954B1
公开(公告)日：2001-11-27
申请号：US09768960
申请日：2001-01-24
申请人： Dwight Stephen Tabb
发明人： Dwight Stephen Tabb
IPC分类号： A10C306
CPC分类号： A01C3/06
摘要： A manure-grinding fertilizer spreader has a spiral-bladed cylinder (1) as a preferred progressively bladed cylinder (17) at a bottom of a funneled mobile hopper (11) with a grinder base (12) proximate a discharge aperture (13). In a small, few-animal embodiment, the mobile funneled hopper is hand-cart sized to be hand-pulled or small-tractor-pulled with the spiral-bladed cylinder being rotated by detachable engagement of wheels (15) with a cutter axle (10) on which the spiral-bladed cylinder is situated axially. In a powered embodiment for all sizes, the spiral-bladed cylinder is rotated by an engine (35) in close proximity to a grinder blade (22) on the grinder base to cut stable-bedding straw and hard manure for pulverized spreading. In large commercial sizes, the funneled hopper can be at an end of a container bed (27) from which manure is belt-conveyed to the mobile hopper for grinding and spreading.
摘要翻译：肥料研磨肥料撒布机在漏斗式移动料斗（11）的底部具有螺旋叶片圆筒（1）作为优选的渐进叶片圆筒（17），其具有靠近排出孔（13）的研磨机基座（12）。在一个小而少动物的实施例中，移动漏斗的料斗是手推车的大小，以手拉或小拖拉机拉动，螺旋叶片的圆筒通过轮（15）与切割轴（15）的可拆卸接合而旋转，螺旋叶片圆柱体在其上轴向位于其上。在用于所有尺寸的动力实施例中，螺旋叶片缸由位于研磨机基座上的研磨刀片（22）附近的发动机（35）旋转，以切割稳定的床垫秸秆和用于粉碎铺展的硬粪肥。在大型商业尺寸中，漏斗式料斗可以在容器床（27）的末端，粪便被带式传送到移动料斗以进行研磨和铺展。

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