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1. 发明申请

WO2005106037A1 A METHOD FOR IDENTIFYING ACTIVATORS OF GENE TRANSCRIPTION 审中-公开
标题翻译：鉴定基因转录激活子的方法
公开(公告)号：WO2005106037A1
公开(公告)日：2005-11-10
申请号：PCT/US2005/012286
申请日：2005-04-11
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , DUVICK, Jonathan, P. , FEDOROVA, Maria
发明人： DUVICK, Jonathan, P. , FEDOROVA, Maria
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6897 , C12N15/1086
摘要： Methods and compositions for identifying a nucleotide sequence that encodes a polypeptide that directly or indirectly modulates the activity of a transcriptional regulatory region are provided. The methods permit the efficient screening of a large number of candidate nucleotide sequences for potential modulators of transcription. In particular, a population of polynucleotide constructs comprising a common transcriptional regulatory region operably linked to a candidate sequence is introduced into a population of host cells, and each candidate sequence is screened for modulation in its expression level, for example, using methods that employ hybridization to a complementary DNA microarray. The expression level of the candidate sequence correlates to the activity of the transcriptional regulatory region. Thus, alterations in the expression level of a candidate sequence indicate that the sequence encodes a polypeptide that modulates the activity of the transcriptional regulatory region. Compositions for practicing the invention are further provided.
摘要翻译：提供了用于鉴定编码直接或间接调节转录调节区活性的多肽的核苷酸序列的方法和组合物。该方法允许有效筛选潜在的转录调控因子的大量候选核苷酸序列。特别地，将包含可操作地连接到候选序列的常见转录调节区的多核苷酸构建体的群体引入宿主细胞群体中，并且筛选每个候选序列以其表达水平进行调节，例如使用采用杂交的方法到互补的DNA微阵列。候选序列的表达水平与转录调控区的活性相关。因此，候选序列的表达水平的改变表明序列编码调节转录调节区活性的多肽。进一步提供实施本发明的组合物。

2. 发明申请

WO0078983A3 SUNFLOWER ANTI-PATHOGENIC PROTEINS AND GENES AND THEIR USES 审中-公开
标题翻译： SUNFLOWER抗病原蛋白和基因及其用途
公开(公告)号：WO0078983A3
公开(公告)日：2001-08-16
申请号：PCT/US0017090
申请日：2000-06-21
申请人： PIONEER HI BRED INT , CURAGEN CORP , BIDNEY DENNIS L , CRASTA OSWALD R , DUVICK JONATHAN P , HU XU , LU GUIHUA
发明人： BIDNEY DENNIS L , CRASTA OSWALD R , DUVICK JONATHAN P , HU XU , LU GUIHUA
IPC分类号： C07K14/415 , C12N9/06 , C12N15/29 , C12N15/53 , C12N15/82 , A01H5/00 , A01N65/00
CPC分类号： C12N9/0032 , C07K14/415 , C12N15/8282
摘要： Compositions and methods to aid in protecting plants from invading pathogenic organisms are provided. The compositions of the invention comprise anti-pathogenic genes, including 5' regulatory sequences in the promoter, and proteins encoded by the anti-pathogenic genes. The compositions find use in methods for reducing or eliminating damage to plants caused by plant pathogens. Transformed plants, plant cells, tissues, and seed having enhanced disease resistance are also provided.
摘要翻译：提供了有助于保护植物免受入侵病原生物体的组合物和方法。本发明的组合物包含抗致病基因，包括启动子中的5'调节序列，以及由抗病原基因编码的蛋白质。该组合物用于减少或消除由植物病原体引起的植物损伤的方法。还提供了具有增强的抗病性的转化植物，植物细胞，组织和种子。

3. 发明申请

WO2000028012A3 THE USE OF β-GLUCOSIDASE TO ENHANCE DISEASE RESISTANCE AND RESISTANCE TO INSECTS IN CROP PLANTS 审中-公开
公开(公告)号：WO2000028012A3
公开(公告)日：2000-05-18
申请号：PCT/US1999/025776
申请日：1999-11-02
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , CURAGEN CORPORATION , DUVICK, Jonathan, P. , SIMMONS, Carl, R. , CRASTA, Oswald, R. , FOLKERTS, Otto
发明人： DUVICK, Jonathan, P. , SIMMONS, Carl, R. , CRASTA, Oswald, R. , FOLKERTS, Otto
IPC分类号： C12N15/82
摘要： Methods for the enhancement of plant disease resistance are provided. The methods comprise transforming said plant with a gene encoding β-glucosidase and increasing the expression said gene above wild-type levels. Transformed plants, plant cells and seeds are provided. Disease resistant transformed plants, plant cells and seeds are also provided.

4. 发明申请

WO0028012A2 THE USE OF beta -GLUCOSIDASE TO ENHANCE DISEASE RESISTANCE AND RESISTANCE TO INSECTS IN CROP PLANTS 审中-公开
标题翻译： β-葡萄糖苷酶对植物抗病性和耐药性的使用
公开(公告)号：WO0028012A2
公开(公告)日：2000-05-18
申请号：PCT/US9925776
申请日：1999-11-02
申请人： PIONEER HI BRED INT , CURAGEN CORP , DUVICK JONATHAN P , SIMMONS CARL R , CRASTA OSWALD R , FOLKERTS OTTO
发明人： DUVICK JONATHAN P , SIMMONS CARL R , CRASTA OSWALD R , FOLKERTS OTTO
IPC分类号： C12N15/56 , C12N15/82 , C12N15/00
CPC分类号： C12N15/8286 , C12N15/8282 , Y02A40/162
摘要： Methods for the enhancement of plant disease resistance are provided. The methods comprise transforming said plant with a gene encoding beta -glucosidase and increasing the expression said gene above wild-type levels. Transformed plants, plant cells and seeds are provided. Disease resistant transformed plants, plant cells and seeds are also provided.
摘要翻译：提供了提高植物抗病性的方法。所述方法包括用编码β-葡糖苷酶的基因转化所述植物，并将所述基因的表达增加到野生型水平以上。提供了转化的植物，植物细胞和种子。还提供了抗病性转化的植物，植物细胞和种子。

5. 发明申请

WO2002070723A3 MAIZE PEROXIDASE GENES AND THEIR USE FOR IMPROVING PLANT DISEASE RESISTANCE AND STALK STRENGTH 审中-公开
公开(公告)号：WO2002070723A3
公开(公告)日：2002-09-12
申请号：PCT/US2002/001454
申请日：2002-01-17
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , DUVICK, Jonathan, P. , MADDOX, Joyce, R. , NAVARRO ACEVEDO, Pedro, A. , SIMMONS, Carl, R.
发明人： DUVICK, Jonathan, P. , MADDOX, Joyce, R. , NAVARRO ACEVEDO, Pedro, A. , SIMMONS, Carl, R.
IPC分类号： C12N9/08
摘要： Methods and compositions for modulating the plant defense response are provided. Nucleotide and amino acid sequences for maize peroxidases are provided. These sequences can be used in expression cassettes for modulating plant defense response, increasing plant disease resistance and increasing plant stalk strenght. These sequences can also be used in methods of selecting or breeding for plants with increased disease resistance. Transformed plants, plant cells, tissues, and seed are also provided.

6. 发明申请

WO0028012A9 THE USE OF beta -GLUCOSIDASE TO ENHANCE DISEASE RESISTANCE AND RESISTANCE TO INSECTS IN CROP PLANTS 审中-公开
标题翻译： β-葡萄糖苷酶对植物抗病性和耐药性的使用
公开(公告)号：WO0028012A9
公开(公告)日：2002-08-29
申请号：PCT/US9925776
申请日：1999-11-02
申请人： PIONEER HI BRED INT , CURAGEN CORP , DUVICK JONATHAN P , SIMMONS CARL R , CRASTA OSWALD R , FOLKERTS OTTO
发明人： DUVICK JONATHAN P , SIMMONS CARL R , CRASTA OSWALD R , FOLKERTS OTTO
IPC分类号： C12N15/56 , C12N15/82 , C12N9/24
CPC分类号： C12N15/8286 , C12N15/8282 , Y02A40/162
摘要： Methods for the enhancement of plant disease resistance are provided. The methods comprise transforming said plant with a gene encoding beta -glucosidase and increasing the expression said gene above wild-type levels. Transformed plants, plant cells and seeds are provided. Disease resistant transformed plants, plant cells and seeds are also provided.
摘要翻译：提供了提高植物抗病性的方法。所述方法包括用编码β-葡糖苷酶的基因转化所述植物，并将所述基因的表达增加到野生型水平以上。提供了转化的植物，植物细胞和种子。还提供了抗病性转化的植物，植物细胞和种子。

7. 发明申请

WO0004158A3 COMPOSITIONS AND METHODS FOR FUMONISIN DETOXIFICATION 审中-公开
标题翻译：组合物和方法用于FUMONISIN脱氧
公开(公告)号：WO0004158A3
公开(公告)日：2000-05-18
申请号：PCT/US9915837
申请日：1999-07-14
申请人： PIONEER HI BRED INT , DUVICK JONATHAN P , MADDOX JOYCE , GILLIAM JACOB
发明人： DUVICK JONATHAN P , MADDOX JOYCE , GILLIAM JACOB
IPC分类号： A23K1/00 , A23K1/165 , A23K3/00 , A23K3/03 , C12N1/21 , C12N5/02 , C12N15/52 , C12N15/82 , A01H5/00 , A01N63/02 , A23L1/015 , C12N5/10
CPC分类号： C12N15/8282 , A23K10/14 , A23K10/16 , A23K10/18 , A23K20/189 , A23K30/00 , A23K30/18 , C12N15/52 , C12N15/8242
摘要： Compositions and methods for the complete detoxification of fumonisin and fumonisin degradation products are provided. Particularly, nucleotide sequences corresponding to the detoxification enzymes are provided. The sequences find use in preparing expression cassettes for the transformation of a broad variety of host cells and organisms.
摘要翻译：提供了伏马菌素和伏马菌素降解产物完全解毒的组合物和方法。特别地，提供了与解毒酶相对应的核苷酸序列。这些序列用于制备用于转化各种宿主细胞和生物体的表达盒。

8. 发明申请

WO0028012A8 THE USE OF beta -GLUCOSIDASE TO ENHANCE DISEASE RESISTANCE AND RESISTANCE TO INSECTS IN CROP PLANTS 审中-公开
标题翻译： β-葡萄糖苷酶对植物抗病性和耐药性的使用
公开(公告)号：WO0028012A8
公开(公告)日：2003-11-06
申请号：PCT/US9925776
申请日：1999-11-02
申请人： PIONEER HI BRED INT , CURAGEN CORP , DUVICK JONATHAN P , SIMMONS CARL R , CRASTA OSWALD R , FOLKERTS OTTO
发明人： DUVICK JONATHAN P , SIMMONS CARL R , CRASTA OSWALD R , FOLKERTS OTTO
IPC分类号： C12N15/56 , C12N15/82 , C12N9/24
CPC分类号： C12N15/8286 , C12N15/8282 , Y02A40/162
摘要： Methods for the enhancement of plant disease resistance are provided. The methods comprise transforming said plant with a gene encoding beta -glucosidase and increasing the expression said gene above wild-type levels. Transformed plants, plant cells and seeds are provided. Disease resistant transformed plants, plant cells and seeds are also provided.
摘要翻译：提供了提高植物抗病性的方法。所述方法包括用编码β-葡糖苷酶的基因转化所述植物，并将所述基因的表达增加到野生型水平以上。提供了转化的植物，植物细胞和种子。还提供了抗病性转化的植物，植物细胞和种子。

9. 发明申请

WO02070723A2 MAIZE PEROXIDASE GENES AND THEIR USE FOR IMPROVING PLANT DISEASE RESISTANCE AND STALK STRENGTH 审中-公开
标题翻译：玉米过氧化物酶基因及其用于改善植物抗病性和耐力的用途
公开(公告)号：WO02070723A2
公开(公告)日：2002-09-12
申请号：PCT/US0201454
申请日：2002-01-17
申请人： PIONEER HI BRED INT , DUVICK JONATHAN P , MADDOX JOYCE R , NAVARRO ACEVEDO PEDRO A , SIMMONS CARL R
发明人： DUVICK JONATHAN P , MADDOX JOYCE R , NAVARRO ACEVEDO PEDRO A , SIMMONS CARL R
IPC分类号： C12N9/08 , C12N15/82
CPC分类号： C12N9/0065 , C12N15/8271 , C12N15/8279
摘要： Methods and compositions for modulating the plant defense response are provided. Nucleotide and amino acid sequences for maize peroxidases are provided. These sequences can be used in expression cassettes for modulating plant defense response, increasing plant disease resistance and increasing plant stalk strenght. These sequences can also be used in methods of selecting or breeding for plants with increased disease resistance. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译：提供了调节植物防御反应的方法和组合物。提供玉米过氧化物酶的核苷酸和氨基酸序列。这些序列可用于调控植物防御反应，提高植物病害抗性和提高植物茎强度的表达盒中。这些序列也可用于选择或育种具有增加的抗病性的植物的方法。还提供了转化的植物，植物细胞，组织和种子。

10. 发明申请

WO2002062964A3 AP1 AMINE OXIDASE VARIANTS 审中-公开
公开(公告)号：WO2002062964A3
公开(公告)日：2002-08-15
申请号：PCT/US2002/003883
申请日：2002-02-06
申请人： MAXYGEN, INC. , PIONEER HI-BRED INTERNATIONAL, INC. , CHATTERJEE, Ranjini , DUVICK, Jonathan, P. , ENGLISH, James
发明人： CHATTERJEE, Ranjini , DUVICK, Jonathan, P. , ENGLISH, James
IPC分类号： C12N9/02 , C12N1/20 , C12N15/00 , C12Q1/00 , C12Q1/34 , C12Q1/68 , C12P21/06 , C12P21/04 , A61K38/44 , C07H21/04 , C07K1/00 , C07K16/00
摘要： New fumonisin detoxifying or fumonisin-derivative detoxifying homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, antibodies to the new homologues, and methods of using the homologues are also provided.

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