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    • 1. 发明申请
    • METHOD FOR CLASSIFYING THE PRESENCE OR ABSENCE OF A MICROORGANISM IN A BIOLOGICAL MEDIUM
    • 分类生物介质中MICROORGANISM的存在或不存在的方法
    • WO2013001465A3
    • 2013-03-28
    • PCT/IB2012053249
    • 2012-06-27
    • COMMISSARIAT ENERGIE ATOMIQUECENTRE NAT RECH SCIENTMARCOUX PIERREDUPOY MATHIEUGUILLEMOT LAURE-HELENETRAN-THI THU-HOA
    • MARCOUX PIERREDUPOY MATHIEUGUILLEMOT LAURE-HELENETRAN-THI THU-HOA
    • C12Q1/04
    • C12Q1/18C12Q1/04C12Q2334/10C12Q2334/30
    • The present invention relates to a method for determining the presence or absence of a microorganism, said method including the steps of: 1) providing an enclosure containing a liquid or semi-solid phase consisting of a biological medium capable of containing a living form of said microorganism, nutritional elements, and an enzymatic substrate which is specific to said microorganism and which can be metabolised into at least one VOC metabolite, and a gaseous phase adjacent to said liquid or semi-solid phase; 2) exposing at least said liquid or semi-solid phase to conditions that are propitious for said microorganism to metabolise said enzymatic substrate into a molecule of said VOC metabolite; and 3) determining, by optical transduction, the presence or absence of said VOC metabolite, characterised in that the latter interacts with a nanoporous matrix, said matrix being implemented in a form that is separate from said enzymatic substrate, and in that the detection, by optical transduction, of a change in the optical properties of said matrix indicates an interaction of said matrix with said metabolite.
    • 本发明涉及一种用于确定微生物存在或不存在的方法,所述方法包括以下步骤:1)提供包含液体或半固体相的外壳,所述液体或半固相由能够容纳所述 微生物,营养元素和对所述微生物特异性并且可以代谢成至少一种VOC代谢物的酶底物和与所述液体或半固体相邻近的气相; 2)将至少所述液体或半固体相暴露于有利于所述微生物将所述酶底物代谢成所述VOC代谢物分子的条件; 3)通过光学转导来确定所述VOC代谢物的存在或不存在,其特征在于后者与纳米多孔基质相互作用,所述基质以与所述酶底物分开的形式实施,并且所述检测, 通过光学转导,所述基质的光学性质的变化表示所述基质与所述代谢物的相互作用。