专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

2. 发明申请

WO0037619A3 MUTANT EPOXIDE HYDROLASES 审中-公开
标题翻译：突变环氧化物水解物
公开(公告)号：WO0037619A3
公开(公告)日：2000-08-10
申请号：PCT/NL9900780
申请日：1999-12-16
申请人： DSM NV , RINK RICK , LUTJE SPELBERG JEFFREY HARALD , NARDINI MARCO , DIJKSTRA BAUKE WIEPKE , KELLOGG RICHARD MORRISON , JANSSEN DIRK BAREND
发明人： RINK RICK , LUTJE SPELBERG JEFFREY HARALD , NARDINI MARCO , DIJKSTRA BAUKE WIEPKE , KELLOGG RICHARD MORRISON , JANSSEN DIRK BAREND
IPC分类号： C12N1/21 , C12N9/14 , C12N15/55 , C12P41/00 , C12N15/70
CPC分类号： C12P41/00 , C12N9/14
摘要： Mutant epoxide hydrolases having an amino acid substitution of a tyrosine residue in the cap domain of the wild type epoxide hydrolase, in particular mutant epoxide hydrolases having an amino acid substitution of the tyrosine residue corresponding to Tyr or Tyr in an epoxide hydrolase with an aminoacid sequence according to SEQ ID No. 1. Preferably the Tyr is substituted by Phe. The invention also relates to nucleic acid sequences encoding such mutant epoxide hydrolases expression constructs wherein such nucleic acid sequence according is operably linked to a regulatory region capable of directing the expression of the mutant epoxide hydrolase in a suitable expression host, vectors capable of transforming a host cell characterized in that the vector containing such expression construct, transformed host cells transformed with such a vector, processes for the preparation of such mutant epoxide hydrolases and processes for the preparation of optically active epoxides and/or diols using such mutant epoxide hydrolases.
摘要翻译：在野生型环氧化物水解酶的帽结构域中具有酪氨酸残基的氨基酸取代的突变型环氧化物水解酶，特别是具有对应于Tyr 152或Tyr <215的酪氨酸残基的氨基酸取代的突变型环氧化物水解酶在具有根据SEQ ID No.1的氨基酸序列的环氧化物水解酶。优选地，Tyr被Phe取代。本发明还涉及编码这种突变型环氧化物水解酶表达构建体的核酸序列，其中这样的核酸序列可操作地连接到能够引导突变型环氧化物水解酶在合适表达宿主中的表达的调控区，能够转化宿主的载体细胞特征在于含有这种表达构建体的载体，用这种载体转化的转化的宿主细胞，用于制备这种突变型环氧化物水解酶，以及使用这种突变型环氧化物水解酶制备光学活性环氧化物和/或二醇的方法。

IPRDB

热门服务

关于我们

友情链接

联系方式