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1. 发明申请

WO2003006994A1 FLUORESCENCE POLARIZATION-BASED HOMOGENOUS ASSAY FOR AFLATOXINS 审中-公开
标题翻译：基于偏振的基于偏磷酸的均质测定法
公开(公告)号：WO2003006994A1
公开(公告)日：2003-01-23
申请号：PCT/US2002/021696
申请日：2002-07-10
申请人： DIACHEMIX LLC
发明人： NASIR, Mohammad, Sarwar , JOLLEY, Michael, E.
IPC分类号： G01N33/58
CPC分类号： G01N33/542 , G01N33/582
摘要： A homogenous assay for determining the aflatoxin content in agricultural products uses the technique of fluorescence polarization. A solvent is used to extract aflatoxins from a sample of the agricultural product. A mixture is prepared by combining the extract with a tracer and with a monoclonal antibody specific for aflatoxin. The tracer is able to bind to the monoclonal antibody to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating an aflatoxin oxime to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The aflatoxin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of aflatoxin solutions of known concentration.
摘要翻译：用于确定农产品中黄曲霉毒素含量的均匀测定法使用荧光偏振技术。使用溶剂从农产品样品中提取黄曲霉毒素。通过将提取物与示踪剂和特异于黄曲霉毒素的单克隆抗体组合来制备混合物。示踪剂能够结合单克隆抗体以产生可检测的荧光偏振变化。通过将黄曲霉毒素与合适的荧光团缀合来制备示踪剂。测量混合物的荧光偏振。可以使用通过测量已知浓度的一系列黄曲霉毒素溶液的荧光偏振获得的标准曲线来计算混合物的黄曲霉毒素浓度。

2. 发明专利

PT1320755E ENSAIO HOMOGÉNEO BASEADO EM POLARIZAÇÃO DE FLUORESCÊNCIA PARA A DETERMINAÇÃO DE DESOXINIVALENOL EM CEREAIS 未知
公开(公告)号：PT1320755E
公开(公告)日：2008-02-18
申请号：PT01975741
申请日：2001-09-10
申请人： DIACHEMIX LLC
发明人： JOLLEY MICHAEL E , NASIR MOHAMMAD SARWAR
IPC分类号： G01N33/569 , G01N33/542

3. 发明专利

DE60222423D1 未知
公开(公告)号：DE60222423D1
公开(公告)日：2007-10-25
申请号：DE60222423
申请日：2002-07-10
申请人： DIACHEMIX LLC
发明人： NASIR MOHAMMAD SARWAR , JOLLEY MICHAEL E
IPC分类号： G01N33/58 , G01N33/533 , G01N33/542 , G01N33/92
摘要： A homogeneous assay for determining the aflatoxin content in agricultural products uses the technique of fluorescence polarization. A solvent is used to extract aflatoxins from a sample of the agricultural product. A mixture is prepared by combining the extract with a tracer and with a monoclonal antibody specific for aflatoxin. The tracer is able to bind to the monoclonal antibody to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating an aflatoxin oxime to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The aflatoxin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of aflatoxin solutions of known concentration.

4. 发明专利

NZ533170A A peptide-based fluorescence polarization assay for detection of antibodies to Mycobacterium bovis 未知
公开(公告)号：NZ533170A
公开(公告)日：2007-01-26
申请号：NZ53317002
申请日：2002-10-31
申请人： DIACHEMIX LLC
发明人： SURUJBALLI OM P , ROMANOWSKA ANNA , JOLLEY MICHAEL E , NASIR MOHAMMAD SARWAR
IPC分类号： C07K14/35 , G01N33/542 , G01N33/569 , G01N33/52 , G01N33/68
摘要： A method for detecting Mycobacterium bovis-infected animals comprises: adding a tracer to a sample from an animal to form a mixture, wherein the tracer is formed by labelling a polypeptide of M. bovis protein MPB70 with a fluorophore, wherein the polypeptide has more than 10 but less than 100 amino acids; measuring the fluorescence polarization of the mixture; and detecting the presence of M. bovis antibodies in the animal from the measured fluorescence polarization of the mixture. Also described is a tracer for detecting M. bovis antibodies in a fluorescence polarization assay, wherein the tracer is formed by labelling a polypeptide of M. bovis protein MPB70 with a fluorophore, wherein the polypeptide has more than 10 but less than 100 amino acids, such that the tracer binds to antibodies specific for M. bovis to produce a detectable change in fluorescence polarization.

5. 发明专利

DE60116118D1 未知
公开(公告)号：DE60116118D1
公开(公告)日：2006-01-26
申请号：DE60116118
申请日：2001-09-10
申请人： DIACHEMIX LLC MILWAUKEE
发明人： NASIR SARWAR , JOLLEY E
IPC分类号： G01N21/64 , G01N33/53 , G01N33/542 , G01N33/569
摘要： A homogeneous assay for determining the fumonisin content in grains uses the technique of fluorescence polarization. A grain extract is prepared by shaking a crushed grain sample with a solvent. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to fumonisin. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating fumonisin to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The fumonisin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of fumonisin solutions of known concentration.

6. 发明专利

ES2215197T3 未知
公开(公告)号：ES2215197T3
公开(公告)日：2004-10-01
申请号：ES96933797
申请日：1996-08-28
申请人： DIACHEMIX LLC
发明人： JOLLEY MICHAEL E , NIELSEN KLAUS H
IPC分类号： G01N33/569 , G01N33/533
摘要： In a homogeneous immunoassay, fluorophore-conjugated lipopolysaccharide derived bacterial antigens are reacted with antibodies specific for the antigens. Quantitative detection of the formation of an immune complex is obtained by measuring the change in fluorescence polarization after complex formation. The reaction occurs quickly (less than two minutes to equilibrium), and involves the addition of only one reagent to a diluted serum specimen. The absence of a solid phase separation step eliminates false positive results and increases throughput.

7. 发明专利

BRPI0211116B1 ensaio homogêneo e kit de ensaio para a determinação de aflatoxinas em produtos agrícolas 未知
公开(公告)号：BRPI0211116B1
公开(公告)日：2015-10-13
申请号：BR0211116
申请日：2002-07-10
申请人： DIACHEMIX LLC
发明人： JOLLEY MICHAEL E , NASIR MOHAMMAD SARWAR
IPC分类号： G01N33/533 , G01N33/542 , G01N33/58 , G01N33/92
摘要： "ensaio homogêneo e kit de ensaio para a determinação de aflatoxinas em produtos agrícolas". um ensaio homogêneo para determinar o teor de aflatoxina em produtos agrícolas usa a técnica de polarização de fluorescência. um solvente é usado para extrair aflatoxinas de uma amostra do produto agrícola. uma mistura é preparada combinando-se o extrato com um rastreador e com um anticorpo monoclonal especifico para aflatoxina. o rastreador é capaz de ligar ao anticorpo monoclonal para produzir uma mudança detectável em polarização de fluorescência. o rastreador é preparado conjugando-se uma oxima de aflatoxina a um fluoroforo adequado. a polarização de fluorescência da mistura é medida. a concentração de aflatoxina da mistura pode ser calculada usando uma curva padrão obtida medindo-se a polarização de fluorescência de uma série de soluções de aflatoxina de concentração conhecida.

8. 发明专利

ES2379774T3 Análisis de polarización por fluorescencia basado en péptidos para la detección de anticuerpos contra Mycobacterium bovis 未知
公开(公告)号：ES2379774T3
公开(公告)日：2012-05-03
申请号：ES02802505
申请日：2002-10-31
申请人： DIACHEMIX LLC
发明人： SURUJBALLI OM , ROMANOWSKA ANNA , JOLLEY MICHAEL , NASIR MOHAMMAD
IPC分类号： A61K39/00 , A61K39/04 , A61K39/40 , C07K14/35 , C12N1/12 , G01N33/53 , G01N33/531 , G01N33/533 , G01N33/542 , G01N33/566 , G01N33/569
摘要： Procedimiento para detectar animales infectados con M. bovis, comprendiendo el procedimiento: añadir un trazador a una muestra de un animal para formar una mezcla, en el que el trazador es un péptido de la proteína MPB70 de M. bovis conjugada con un fluoróforo y en el que el péptido está constituido por una secuencia de aminoácidos seleccionada de entre el grupo constituido por la SEC. ID. nº 2 y SEC. ID. nº 6; medir la polarización por fluorescencia de la mezcla; y detectar la presencia de anticuerpos contra M. Bovis en el animal procedente de la polarización por fluorescencia medida de la mezcla.

9. 发明专利

CA2422089C FLUORESCENCE POLARIZATION-BASED HOMOGENEOUS ASSAY FOR DEOXYNIVALENOL DETERMINATION IN GRAINS 未知
公开(公告)号：CA2422089C
公开(公告)日：2009-03-24
申请号：CA2422089
申请日：2001-09-10
申请人： DIACHEMIX LLC
发明人： JOLLEY MICHAEL E , NASIR MOHAMMAD SARWAR
IPC分类号： G01N33/542 , G01N33/10 , G01N33/52 , G01N33/569
摘要： a homogeneous assay for determining the deoxynivanlenol (DON) content in grains uses the technique of fluorescence polarization. A grain extract is prepared by shaking a crushed grain sample with water. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to DON. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating DON to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The DON concentration of the mixtur e may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of DON solutions of known concentratio n.

10. 发明专利

ES2292787T3 ENSAYO HOMOGENEO BASADO EN LA POLIMERIZACION DE FLUORESCENCIA PARA LAS AFLATOXINAS. 未知
公开(公告)号：ES2292787T3
公开(公告)日：2008-03-16
申请号：ES02752236
申请日：2002-07-10
申请人： DIACHEMIX LLC
发明人： NASIR MOHAMMAD SARWAR , JOLLEY MICHAEL E
IPC分类号： G01N33/58 , G01N33/533 , G01N33/542 , G01N33/92
摘要： Ensayo homogéneo para la determinación de las aflatoxinas en productos agrícolas, comprendiendo dicho ensayo homogéneo las etapas que consisten en: combinar un extracto de aflatoxina con un trazador y un anticuerpo para proporcionar una mezcla, siendo dicho anticuerpo específico para la aflatoxina, comprendiendo dicho trazador una oxima de aflatoxina conjugada a un fluoróforo seleccionado de entre el grupo constituido por fluoresceinamina, 5-aminoacetil-amidofluoresceína, y 5-(5-aminopentil)-tioureidil fluoresceína, pudiendo dicho trazador unirse a dicho anticuerpo para producir un cambio detectable en la polarización de fluorescencia; medir la polarización de fluorescencia de dicha mezcla para obtener una medida de la polarización de fluorescencia medida; y comparar dicha polarización de fluorescencia medida con un valor de la polarización de fluorescencia caracterizado, correspondiendo dicho valor de la polarización de fluorescencia caracterizado a una concentración de la aflatoxina conocida.

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