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1. 发明申请

WO2005113759A2 MICROBIAL PRODUCTION OF XYLITOL VIA A HEXOSE PHOSPHATE AND A PENTOSE PHOSPHATE INTERMEDIATE 审中-公开
标题翻译：通过磷酸氢铵和磷酸氢盐中间体生产XYLITOL的微生物
公开(公告)号：WO2005113759A2
公开(公告)日：2005-12-01
申请号：PCT/US2005/017550
申请日：2005-05-19
申请人： BIOTECHNOLOGY RESEARCH AND DEVELOPMENT CORPORATION , ARGRICULTURAL RESEARCH SERVICE, UNITED STATES DEPARTEMENT OF AGRICULTURE , FOTHERINGHAM, Ian , TAYLOR, Paul , DEMIRJIAN, David , OSWALD, Nick, Edinburgh
发明人： FOTHERINGHAM, Ian , TAYLOR, Paul , DEMIRJIAN, David , OSWALD, Nick, Edinburgh
IPC分类号： C12N9/00
CPC分类号： C12P19/02 , C12P7/18 , C12P9/00
摘要： The invention provides methods and compositions for production of xylitol, xylose, or combinations thereof from simple sugars from biosynthetic pathway that utilize D-fructose-6-phosphate and D-xylulose-5-phosphate as pathway intermediates.
摘要翻译：本发明提供了从利用D-果糖-6-磷酸和D-木酮糖-5-磷酸作为途径中间体的生物合成途径的简单糖生产木糖醇，木糖或其组合的方法和组合物。

2. 发明申请

WO0118217A3 THERMUS PROMOTERS FOR GENE EXPRESSION 审中-公开
标题翻译：用于基因表达的THERMUS启动子
公开(公告)号：WO0118217A3
公开(公告)日：2001-12-06
申请号：PCT/US0024430
申请日：2000-09-06
申请人： THERMOGEN INC , PEREDELTCHOUK MIKHAIL , VONSTEIN VERONIKA , DEMIRJIAN DAVID
发明人： PEREDELTCHOUK MIKHAIL , VONSTEIN VERONIKA , DEMIRJIAN DAVID
IPC分类号： C12N1/21 , C12N15/74 , C12N15/11 , C12Q1/68
CPC分类号： C12N15/74
摘要： The present invention relates to a system for identifying, isolating and utilizing promoter elements useful for expression of nucleotide sequences and the proteins encoded thereby in a thermophile. In one embodiment, a recombinant DNA molecule is provided, and comprises a reporter sequence, a putative thermophile promoter, a selectable marker sequence, and a 3' and a 5' DNA targeting sequence that are together capable of causing integration of at least a portion of said DNA molecule into the genome of a thermophile. Further, within the recombinant DNA, the reporter sequence is under the transcriptional control of a promoter which functions in a thermophile to form a promoter/reporter cassette, the promoter/reporter cassette is flanked by said 3' and said 5'DNA targeting sequences, and the promoter/reporter cassette is positioned in the opposite orientation of the DNA targeting sequences.
摘要翻译：本发明涉及用于鉴定，分离和利用在嗜热菌中用于表达核苷酸序列和由其编码的蛋白质的启动子元件的系统。在一个实施方案中，提供了重组DNA分子，其包含报道序列，推定的嗜热性启动子，选择标记序列和3'和5'DNA靶向序列，它们一起能够引起至少一部分所述DNA分子进入嗜热菌基因组。此外，在重组DNA中，报道子序列处于启动子的转录控制下，所述启动子在嗜热生物中起作用形成启动子/报道子盒，启动子/报道子盒侧接所述3'和所述5'DNA靶向序列，并且启动子/报道子盒定位在DNA靶向序列的相反方向。

3. 发明申请

WO2012024678A3 ACTIVATED SUGARS 审中-公开
公开(公告)号：WO2012024678A3
公开(公告)日：2012-02-23
申请号：PCT/US2011/048642
申请日：2011-08-22
申请人： ZUCHEM, INC. , WOODYER, Ryan , TAYLOR, Paul , DEMIRJIAN, David
发明人： WOODYER, Ryan , TAYLOR, Paul , DEMIRJIAN, David
IPC分类号： C12N9/12
摘要： Kinase and nucleotidyltransferase enzymes for the production of activated sugars have been developed. These enzymes have improved stability for industrial application and relaxed specificity towards a variety of sugars. These enzymes are useful in, for example, the production of diverse NDP-sugars for glycosylation of aglycones of interest, production of oligosaccharides, production of other important glycoylated sugars, and in drug discovery applications.

4. 发明申请

WO2005113759A3 MICROBIAL PRODUCTION OF XYLITOL VIA A HEXOSE PHOSPHATE AND A PENTOSE PHOSPHATE INTERMEDIATE 审中-公开
标题翻译：通过磷酸氢铵和磷酸氢盐中间体生产XYLITOL的微生物
公开(公告)号：WO2005113759A3
公开(公告)日：2006-12-21
申请号：PCT/US2005017550
申请日：2005-05-19
申请人： BIOTECHNOLOGY RES & DEV , ARGRICULTURAL RES SERVICE UNIT , FOTHERINGHAM IAN , TAYLOR PAUL , DEMIRJIAN DAVID , OSWALD NICK EDINBURGH
发明人： FOTHERINGHAM IAN , TAYLOR PAUL , DEMIRJIAN DAVID , OSWALD NICK EDINBURGH
IPC分类号： C12N1/21 , C12N9/00 , C12N9/04 , C12N9/10 , C12N9/12 , C12N9/92 , C12P7/18 , C12P9/00 , C12P19/02
CPC分类号： C12P19/02 , C12P7/18 , C12P9/00
摘要： The invention provides methods and compositions for production of xylitol, xylose, or combinations thereof from simple sugars from biosynthetic pathway that utilize D-fructose-6-phosphate and D-xylulose-5-phosphate as pathway intermediates.
摘要翻译：本发明提供了从利用D-果糖-6-磷酸和D-木酮糖-5-磷酸作为途径中间体的生物合成途径的简单糖生产木糖醇，木糖或其组合的方法和组合物。

5. 发明申请

WO2005113759A8 MICROBIAL PRODUCTION OF XYLITOL VIA A HEXOSE PHOSPHATE AND A PENTOSE PHOSPHATE INTERMEDIATE 审中-公开
标题翻译：通过磷酸一钙和磷酸戊糖酯微生物生产木糖醇
公开(公告)号：WO2005113759A8
公开(公告)日：2006-06-08
申请号：PCT/US2005017550
申请日：2005-05-19
申请人： BIOTECHNOLOGY RES & DEV , RTEMENT OF AGRICULTURE ARGRICU , FOTHERINGHAM IAN , TAYLOR PAUL , DEMIRJIAN DAVID , OSWALD NICK EDINBURGH
发明人： FOTHERINGHAM IAN , TAYLOR PAUL , DEMIRJIAN DAVID , OSWALD NICK EDINBURGH
IPC分类号： C12N9/00 , C12P7/18 , C12P9/00 , C12P19/02
CPC分类号： C12P19/02 , C12P7/18 , C12P9/00
摘要： The invention provides methods and compositions for production of xylitol, xylose, or combinations thereof from simple sugars from biosynthetic pathway that utilize D-fructose-6-phosphate and D-xylulose-5-phosphate as pathway intermediates.
摘要翻译：本发明提供了从利用D-果糖-6-磷酸和D-木酮糖-5-磷酸作为途径中间体的来自生物合成途径的单糖生产木糖醇，木糖或其组合的方法和组合物。

6. 发明申请

WO9921971A9 THERMOSTABLE ALCOHOL DEHYDROGENASES 审中-公开
标题翻译：耐热酒精脱水剂
公开(公告)号：WO9921971A9
公开(公告)日：1999-08-19
申请号：PCT/US9822607
申请日：1998-10-26
申请人： THERMOGEN INC , ALLEN LARRY , BRIKUN IGOR , ALKENS JOHN H , DEMIRJIAN DAVID C , MATUR RAMESH , NIKOLSKY YURI , ROZZELL DAVID
发明人： ALLEN LARRY , BRIKUN IGOR , ALKENS JOHN H , DEMIRJIAN DAVID C , MATUR RAMESH , NIKOLSKY YURI , ROZZELL DAVID
IPC分类号： C12N9/04
CPC分类号： C12N9/0006 , C12Y101/01001
摘要： The present invention provides proteins having alcohol dehydrogenase activity. In a particular embodiment, proteins having chiral alcohol selective alcohol dehydrogenase activity. In another embodiment, proteins having thermostable alcohol dehydrogenase activity are provided.
摘要翻译：本发明提供具有醇脱氢酶活性的蛋白质。在具体实施方案中，具有手性醇选择性醇脱氢酶活性的蛋白质。在另一个实施方案中，提供了具有耐热醇脱氢酶活性的蛋白质。

7. 发明申请

WO2012024678A9 ACTIVATED SUGARS 审中-公开
标题翻译：活性糖
公开(公告)号：WO2012024678A9
公开(公告)日：2013-03-21
申请号：PCT/US2011048642
申请日：2011-08-22
申请人： ZUCHEM INC , WOODYER RYAN , TAYLOR PAUL , DEMIRJIAN DAVID
发明人： WOODYER RYAN , TAYLOR PAUL , DEMIRJIAN DAVID
IPC分类号： C12P19/30
CPC分类号： C12N9/1241 , C12N9/1205 , C12P19/02 , C12P19/18 , C12P19/38 , C12Y207/07
摘要： Kinase and nucleotidyltransferase enzymes for the production of activated sugars have been developed. These enzymes have improved stability for industrial application and relaxed specificity towards a variety of sugars. These enzymes are useful in, for example, the production of diverse NDP-sugars for glycosylation of aglycones of interest, production of oligosaccharides, production of other important glycoylated sugars, and in drug discovery applications.
摘要翻译：已经开发了用于生产活化糖的激酶和核苷酸转移酶。这些酶在工业应用中具有改善的稳定性并且对各种糖具有宽松的特异性。这些酶可用于例如用于感兴趣的糖苷配基的糖基化，寡糖的生产，其他重要的糖基化糖的生产以及药物发现应用中的各种NDP-糖的生产。

8. 发明申请

WO2012024678A2 ACTIVATED SUGARS 审中-公开
标题翻译：活性糖
公开(公告)号：WO2012024678A2
公开(公告)日：2012-02-23
申请号：PCT/US2011048642
申请日：2011-08-22
申请人： ZUCHEM INC , WOODYER RYAN , TAYLOR PAUL , DEMIRJIAN DAVID
发明人： WOODYER RYAN , TAYLOR PAUL , DEMIRJIAN DAVID
IPC分类号： C12P19/30
CPC分类号： C12N9/1241 , C12N9/1205 , C12P19/02 , C12P19/18 , C12P19/38 , C12Y207/07
摘要： Kinase and nucleotidyltransferase enzymes for the production of activated sugars have been developed. These enzymes have improved stability for industrial application and relaxed specificity towards a variety of sugars. These enzymes are useful in, for example, the production of diverse NDP-sugars for glycosylation of aglycones of interest, production of oligosaccharides, production of other important glycoylated sugars, and in drug discovery applications.
摘要翻译：已经开发了用于生产活性糖的激酶和核苷酸转移酶。这些酶具有改善的工业应用稳定性和对各种糖的放宽特异性。这些酶可用于例如生产用于目标糖苷配基糖基化的多种NDP-糖，寡糖的产生，其它重要的糖基化糖的生产以及药物发现应用中。

9. 发明申请

WO2005113774A3 METHODS FOR PRODUCTION OF XYLITOL IN MICROORGANISMS 审中-公开
公开(公告)号：WO2005113774A3
公开(公告)日：2005-12-01
申请号：PCT/US2005/017567
申请日：2005-05-19
申请人： BIOTECHNOLOGY RESEARCH AND DEVELOPMENT CORPORATION , AGRICULTURAL RESEARCH SERVICE, UNITED STATES DEPARTMENT OF AGRICULTURE , TAYLOR, Paul , FOTHERINGHAM, Ian , WYMER, Nathan , SAHA, Badal , DEMIRJIAN, David , SAKAKIBARA, Yoshikiyo , RACINE, Francis, Michael
发明人： TAYLOR, Paul , FOTHERINGHAM, Ian , WYMER, Nathan , SAHA, Badal , DEMIRJIAN, David , SAKAKIBARA, Yoshikiyo , RACINE, Francis, Michael
IPC分类号： C12N1/21 , C12N9/04 , C12N9/12 , C12N9/92 , C12P7/18 , C12P19/02 , C07K14/245
摘要： The invention provides biosynthetic routes to xylitol production that do not require pure D-xylose for synthesis and that can utilize inexpensive substrates such as hemicellulose hydrolysates.

10. 发明申请

WO2005113774A2 METHODS FOR PRODUCTION OF XYLITOL IN MICROORGANISMS 审中-公开
标题翻译：在微生物中生产XYLITOL的方法
公开(公告)号：WO2005113774A2
公开(公告)日：2005-12-01
申请号：PCT/US2005017567
申请日：2005-05-19
申请人： BIOTECHNOLOGY RES & DEV , AGRICULTURAL RES SERVICE UNITE , TAYLOR PAUL , FOTHERINGHAM IAN , WYMER NATHAN , SAHA BADAL , DEMIRJIAN DAVID , SAKAKIBARA YOSHIKIYO , RACINE FRANCIS MICHAEL
发明人： TAYLOR PAUL , FOTHERINGHAM IAN , WYMER NATHAN , SAHA BADAL , DEMIRJIAN DAVID , SAKAKIBARA YOSHIKIYO , RACINE FRANCIS MICHAEL
IPC分类号： C12N9/00 , C12N9/04 , C12N9/10 , C12N9/12 , C12N9/92 , C12N15/52 , C12P7/18 , C12P19/02
CPC分类号： C12N9/1223 , C12N9/0006 , C12N9/1022 , C12N9/1205 , C12N9/92 , C12P7/18 , C12P19/02 , C12Y101/01009 , C12Y202/01001 , C12Y207/01017 , C12Y207/03009 , C12Y503/01005
摘要： The invention provides biosynthetic routes to xylitol production that do not require pure D-xylose for synthesis and that can utilize inexpensive substrates such as hemicellulose hydrolysates.
摘要翻译：本发明提供了生产木糖醇生产的途径，其不需要纯D-木糖用于合成，并且可以利用廉价的底物如半纤维素水解产物。

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